Productive common light chain libraries yield diverse panels of high affinity bispecific antibodies

The making of bispecific antibodies

(2017) Ulrich Brinkmann et al.   mAbs

Biophysical properties of the clinical-stage antibody landscape

(2017) Tushar Jain et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Generation of human bispecific common light chain antibodies by combining animal immunization and yeast display

(2017) Simon Krah et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Efficient Generation of Bispecific Murine Antibodies for Pre-Clinical Investigations in Syngeneic Rodent Models

(2017) Aran F. Labrijn et al.   Scientific Reports

Durable antitumor responses to CD47 blockade require adaptive immune stimulation

(2016) Jonathan T. Sockolosky et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Conformation of the Human Immunoglobulin G2 Hinge Imparts Superagonistic Properties to Immunostimulatory Anticancer Antibodies

(2015) Ann L. White et al.   CANCER CELL

Tuning Cytokine Receptor Signaling by Re-orienting Dimer Geometry with Surrogate Ligands

(2015) Ignacio Moraga et al.   CELL

A Novel Antibody Engineering Strategy for Making Monovalent Bispecific Heterodimeric IgG Antibodies by Electrostatic Steering Mechanism

(2015) Zhi Liu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Improving target cell specificity using a novel monovalent bispecific IgG design

(2015) Yariv Mazor et al.   mAbs

Assessing kinetic and epitopic diversity across orthogonal monoclonal antibody generation platforms

(2015) Yasmina Noubia Abdiche et al.   mAbs

Alternative molecular formats and therapeutic applications for bispecific antibodies

(2015) Christoph Spiess et al.   MOLECULAR IMMUNOLOGY

Exploiting light chains for the scalable generation and platform purification of native human bispecific IgG

(2015) Nicolas Fischer et al.   Nature Communications

A novel, native-format bispecific antibody triggering T-cell killing of B-cells is robustly active in mouse tumor models and cynomolgus monkeys

(2015) Eric J. Smith et al.   Scientific Reports

Automated high-throughput protein purification using an ÄKTApurifier and a CETAC autosampler

(2014) Daniel Yoo et al.   JOURNAL OF CHROMATOGRAPHY A

Generation of bispecific IgG antibodies by structure-based design of an orthogonal Fab interface

(2014) Steven M Lewis et al.   NATURE BIOTECHNOLOGY

In-depth determination and analysis of the human paired heavy- and light-chain antibody repertoire

(2014) Brandon J DeKosky et al.   NATURE MEDICINE

Controlled Fab-arm exchange for the generation of stable bispecific IgG1

(2014) Aran F Labrijn et al.   Nature Protocols

A fully synthetic human Fab antibody library based on fixed VH/VL framework pairings with favorable biophysical properties

(2013) Thomas Tiller et al.   mAbs

Bispecific antibodies with natural architecture produced by co-culture of bacteria expressing two distinct half-antibodies

(2013) Christoph Spiess et al.   NATURE BIOTECHNOLOGY

Efficient generation of stable bispecific IgG1 by controlled Fab-arm exchange

(2013) A. F. Labrijn et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Addressing polyspecificity of antibodies selected from an in vitro yeast presentation system: a FACS-based, high-throughput selection and analytical tool

(2013) Y. Xu et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Structural Basis for Eliciting a Cytotoxic Effect in HER2-Overexpressing Cancer Cells via Binding to the Extracellular Domain of HER2

(2013) Christian Jost et al.   STRUCTURE

Targeting of 4-1BB by monoclonal antibody PF-05082566 enhances T-cell function and promotes anti-tumor activity

(2012) Timothy S. Fisher et al.   CANCER IMMUNOLOGY IMMUNOTHERAPY

Generating Bispecific Human IgG1 and IgG2 Antibodies from Any Antibody Pair

(2012) Pavel Strop et al.   JOURNAL OF MOLECULAR BIOLOGY

Antigen-binding site anatomy and somatic mutations in antibodies that recognize different types of antigens

(2012) Gopalan Raghunathan et al.   JOURNAL OF MOLECULAR RECOGNITION

Synthetic antibodies: Concepts, potential and practical considerations

(2012) S. Miersch et al.   METHODS

Antitumor Activity of a Novel Bispecific Antibody That Targets the ErbB2/ErbB3 Oncogenic Unit and Inhibits Heregulin-Induced Activation of ErbB3

(2012) C. F. McDonagh et al.   MOLECULAR CANCER THERAPEUTICS

A bispecific antibody to factors IXa and X restores factor VIII hemostatic activity in a hemophilia A model

(2012) Takehisa Kitazawa et al.   NATURE MEDICINE

Synthetic Antibodies Designed on Natural Sequence Landscapes

(2011) Wenwu Zhai et al.   JOURNAL OF MOLECULAR BIOLOGY

A stable IgG-like bispecific antibody targeting the epidermal growth factor receptor and the type I insulin-like growth factor receptor demonstrates superior anti-tumor activity

(2011) Jianying Dong et al.   mAbs

Multiparameter RNA and Codon Optimization: A Standardized Tool to Assess and Enhance Autologous Mammalian Gene Expression

(2011) Stephan Fath et al.   PLoS One

Boosting Brain Uptake of a Therapeutic Antibody by Reducing Its Affinity for a Transcytosis Target

(2011) Y. J. Yu et al.   Science Translational Medicine

The Use of Surrogate Antibodies to Evaluate the Developmental and Reproductive Toxicity Potential of an Anti-TNFα PEGylated Fab′ Monoclonal Antibody

(2011) Ian Wakefield et al.   TOXICOLOGICAL SCIENCES

Development of a Two-part Strategy to Identify a Therapeutic Human Bispecific Antibody That Inhibits IgE Receptor Signaling

(2010) Janet Jackman et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Gene optimization mechanisms: A multi-gene study reveals a high success rate of full-length human proteins expressed inEscherichia coli

(2010) Barbara Maertens et al.   PROTEIN SCIENCE

Immunotherapy of lymphoma and leukemia with T-cell engaging BiTE antibody blinatumomab

(2009) Dirk Nagorsen et al.   LEUKEMIA & LYMPHOMA

Precise determination of the diversity of a combinatorial antibody library gives insight into the human immunoglobulin repertoire

(2009) J. Glanville et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A novel solid phase technology for high-throughput gene synthesis

(2008) Jan Van den Brulle et al.   BIOTECHNIQUES