Article
Virology
Jonathan L. Sanchez, Niloofar Ghadirian, Nancy C. Horton
Summary: This paper presents two new structures of the N-terminal domain of the main replication protein, NS1, from human parvovirus B19 (B19V). The NS1-nuc domain plays a crucial role in the rolling hairpin replication of the B19V DNA genome by recognizing origin of replication sequences in double-stranded DNA and cleaving single-stranded DNA at the terminal resolution site (trs). The high-resolution structures of NS1-nuc provide insights into DNA recognition and cleavage by B19V NS1-nuc and predict the important residues involved in DNA cleavage and sequence-specific recognition at the viral origin of replication.
JOURNAL OF VIROLOGY
(2022)
Article
Virology
Kang Ning, Anuradha Roy, Fang Cheng, Peng Xu, Steve Kleiboeker, Carlos R. Escalante, Jingxin Wang, Jianming Qiu
Summary: This study developed a high-throughput screening assay for identifying antivirals against B19V replication and discovered a purine derivative that exhibited significant antiviral activity against B19V infection.
JOURNAL OF VIROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yixi Zhang, Zhiwei Shao, Yanqing Gao, Boming Fan, Jie Yang, Xi Chen, Xin Zhao, Qiyuan Shao, Weizhen Zhang, Chulei Cao, Hehua Liu, Jianhua Gan
Summary: Infection of human parvovirus B19 can lead to various diseases, and the NS1_Nuc protein plays a crucial role in viral DNA replication through its unique binding mode with single-stranded DNA.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Oncology
Li Gong, Jing Tian, Yan Zhang, Zheng Feng, Qiannan Wang, Yan Wang, Fuqin Zhang, Wei Zhang, Gaosheng Huang
Summary: The presence of germinal centers (GCs) in thymomas is closely associated with the pathogenesis of myasthenia gravis (MG), and infection by human parvovirus B19 (B19V) may contribute to the formation of ectopic GCs in thymoma. This study provides new insights into the etiopathogenic mechanism of MG and the role of B19V in thymoma-associated MG.
ANNALS OF SURGICAL ONCOLOGY
(2023)
Article
Virology
Massimo De Paschale, Claudia Pavia, Teresa Cerulli, Debora Cagnin, Maria T. Manco, Luisa Belvisi, Alessia Paganini, Laura Pogliani, Elena Ceriani, Alessandro Porta, Luciana Parola, Paola Mirri, Bianca Osnaghi, Laura Vismara, Pierangelo Clerici
Summary: Parvovirus B19 (B19V) infection in pregnancy is common in Italy, with higher IgG seroprevalence among Italian women compared to non-Italian women. The prevalence of viremia is low and does not lead to significant fetal damage at birth.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Virology
Alessandro Reggiani, Andrea Avati, Francesca Valenti, Erika Fasano, Gloria Bua, Elisabetta Manaresi, Giorgio Gallinella
Summary: In this study, a derived B19V minigenome was designed and produced, which was capable of replication, transcription, and production of NS1 protein in cells, and could complement defective genomes. This provides a valuable tool for studying viral expression and genetics.
Article
Virology
Liting Shao, Kang Ning, Jianke Wang, Fang Cheng, Shengqi Wang, Jianming Qiu
Summary: In this study, we discovered a direct interaction between the large nonstructural protein NS1 of parvovirus and Ku70, which is critical for HBoV1 replication.
JOURNAL OF VIROLOGY
(2022)
Article
Immunology
Yedong Yu, Chunchun Wei, Junhao Lyu, Xiaoliang Wu, Rending Wang, Hongfeng Huang, Jianyong Wu, Jianghua Chen, Wenhan Peng
Summary: This retrospective study evaluated donor-derived B19V infections in kidney transplantations from January 2016 to December 2020. Results showed 0.4% and 1.5% incidence rates in living and deceased donor kidney transplantations, respectively. While some recipients developed infections, they were effectively controlled with treatment, indicating that routine screening for B19V in donors may not be necessary and kidneys from donors with B19V infection can be accepted for transplantation.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Virology
Weiran Shen, Zekun Wang, Kang Ning, Fang Cheng, John F. Engelhardt, Ziying Yan, Jianming Qiu
Summary: Our study demonstrates that productive parvovirus DNA replication can occur independently of hairpin transfer. Through experiments with human bocavirus 1 (HBoV1) duplex genomes, we show that viruses can be generated and infect human airway epithelium without the need for hairpin sequences. This discovery may have implications in the development of parvovirus-based viral vectors with alternative properties.
JOURNAL OF VIROLOGY
(2021)
Review
Immunology
Xi Hu, Chen Jia, Jianyong Wu, Jian Zhang, Zhijie Jiang, Kuifen Ma
Summary: This review discusses the clinical manifestations and current treatment options for Parvovirus B19 (B19V) infection. It highlights the lack of specific therapeutic methods for B19V and emphasizes the importance of identifying potential targets by understanding the virus's life cycle. The review also presents antiviral agents that block viral invasion and inhibit enzymes or regulatory proteins involved in DNA synthesis, and outlines the applications of nanotechnology in B19V treatment.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Infectious Diseases
Trine H. Mogensen, Morten Kelder Skouboe, Ingolf Molle
Summary: Human infection with parvovirus B19 can lead to various clinical manifestations, and treatment can be challenging. This case report presents a 43-year-old man with persistent parvovirus B19 infection and anaemia. The patient showed resistance to standard treatments but responded well to pegylated interferon alfa-2a. This highlights the potential of interferon alfa-2a as a treatment option for persistent parvovirus B19 infection.
LANCET INFECTIOUS DISEASES
(2023)
Article
Virology
Wei Zou, Kang Ning, Peng Xu, Xuefeng Deng, Fang Cheng, Steve Kleiboeker, Jianming Qiu
Summary: The study revealed that the 5-68 amino acid sequence of VP1 can effectively inhibit B19V infection, independent of dimerization but likely dependent on three predicted alpha-helices. This specific sequence may function as a receptor-binding ligand during virus entry, making it a potential antiviral peptide candidate for combating B19V infection.
JOURNAL OF VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ieva Rinkunaite, Egidijus Simoliunas, Daiva Bironaite, Rasa Rutkiene, Virginija Bukelskiene, Rolandas Meskys, Julius Bogomolovas
Summary: The unique region VP1u plays a crucial role in B19V's tropism for erythroid precursor cells, but its interaction with endothelial cells leads to upregulation of cellular stress signaling pathways, potentially causing endothelial damage during acute B19V infection.
Article
Medicine, General & Internal
Bijun Xu, Weimin Zhang, Ximing Qian, Shiqiang Wang, Fan He
Summary: Patients may develop severe anemia due to B19V infection after heart transplantation, with diagnosis relying on viral DNA detection and successful treatment achieved with intravenous immunoglobulin.
Article
Immunology
Hidehiko Suzuki, Takafumi Noguchi, Keiji Ogawa, Paola Miyazato, Yu Hatakeyama, Eiji Morita, Hirotaka Ebina
Summary: The fusion of RBD with PspA enhances neutralizing antibody production, providing protective immunity against both B19 and Streptococcus pneumoniae infections. T cells specific to PspA support immunoglobulin class switching of both RBD and PspA-specific B cells.
Review
Neurosciences
Yomna Badawi, Hiroshi Nishimune
NEUROSCIENCE RESEARCH
(2018)
Article
Biotechnology & Applied Microbiology
Ziying Yan, Wei Zou, Zehua Feng, Weiran Shen, Soo Yeun Park, Xuefeng Deng, Jianming Qiu, John F. Engelhardt
HUMAN GENE THERAPY
(2019)
Article
Virology
Wei Zou, Min Xiong, Xuefeng Deng, John F. Engelhardt, Ziying Yan, Jianming Qiu
Article
Neurosciences
Yomna Badawi, Hiroshi Nishimune
NEUROSCIENCE LETTERS
(2020)
Article
Genetics & Heredity
Xuefeng Deng, Wei Zou, Ziying Yan, Jianming Qiu
Review
Neurosciences
Yomna Badawi, Hiroshi Nishimune
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2020)
Article
Multidisciplinary Sciences
Sudheer K. Tungtur, Heather M. Wilkins, Robert S. Rogers, Yomna Badawi, Jessica M. Sage, Abdulbaki Agbas, Omar Jawdat, Richard J. Barohn, Russell H. Swerdlow, Hiroshi Nishimune
Summary: Treatment with oxaloacetate improved neuromuscular function and delayed limb paralysis in ALS mice, although it did not significantly impact lifespan. The beneficial effects may be attributed to the regulation of neuroinflammation or bioenergetic stress.
SCIENTIFIC REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Scott P. Ginebaugh, Yomna Badawi, Tyler B. Tarr, Stephen D. Meriney
Summary: The mouse neuromuscular junction (NMJ) has been widely used as a model synapse to study neurotransmission. Understanding the relationships within the highly organized active zones (AZs) is crucial for healthy synapses and the pathophysiology of neuromuscular diseases.
Article
Multidisciplinary Sciences
Kang Ning, Wei Zou, Peng Xu, Fang Cheng, Elizabeth Yan Zhang, Aaron Zhang-Chen, Steve Kleiboeker, Jianming Qiu
Summary: The study identified AXL as a protein receptor for B19V to infect human erythroid progenitor cells. Knocking down the AXL gene significantly reduced B19V internalization and replication. The interaction between B19V VP1u and the extracellular domain of AXL was confirmed, suggesting the importance of AXL as a co-receptor for B19V infection.
Article
Biochemistry & Molecular Biology
Kristine S. Ojala, Cassandra J. Kaufhold, Mykenzie R. Davey, Donggyun Yang, Mary Liang, Peter Wipf, Yomna Badawi, Stephen D. Meriney
Summary: Spinal muscular atrophy (SMA) is a monogenic disease that causes severe muscle weakness due to neurotransmission defects and motoneuron degeneration. In a mouse model of severe SMA, the calcium channel gating modifier GV-58, alone or in combination with the potassium channel antagonist 3,4-diaminopyridine (3,4-DAP), significantly improved neuromuscular function and strength. These findings suggest that GV-58 may be a promising treatment for SMA and that adding 3,4-DAP could enhance its effects.
HUMAN MOLECULAR GENETICS
(2023)
Article
Neurosciences
Scott P. Ginebaugh, Yomna Badawi, Rozita Laghaei, Glenn Mersky, Caleb J. Wallace, Tyler B. Tarr, Cassandra Kaufhold, Stephen Reddel, Stephen D. Meriney
Summary: We used a computational model to investigate the pathophysiology of Lambert-Eaton myasthenic syndrome (LEMS) at the neuromuscular junction. The model suggests that disruptions in presynaptic active zone organization and protein content, particularly synaptotagmin, play an important role in LEMS pathophysiology. These findings indicate that a collection of pathological alterations to active zones at the neuromuscular junction may be responsible for LEMS, rather than a simple loss of calcium channels.
JOURNAL OF NEUROPHYSIOLOGY
(2023)
Article
Urology & Nephrology
Steven D. Funk, Raymond H. Bayer, Karen K. McKee, Kazushi Okada, Hiroshi Nishimune, Peter D. Yurchenco, Jeffrey H. Miner
KIDNEY INTERNATIONAL
(2020)