Article
Biotechnology & Applied Microbiology
Dolly Rani, Visarut Buranasudja, Khwanlada Kobtrakul, Wanchai De-Eknamkul, Sornkanok Vimolmangkang
Summary: This study investigated the effects of salicylic acid, chitosan, and methyl jasmonate on bioactive compounds in suspension cells of Pueraria candollei var. mirifica, with salicylic acid showing the most promising results. The extracts from salicylic acid-elicited cells exhibited the highest antioxidant activity and promoted fibroblast proliferation, indicating potential antiaging activities of Pueraria candollei var. mirifica extracts.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Agricultural Engineering
Dolly Rani, Khwanlada Kobtrakul, Wanchai De-Eknamkul, Sornkanok Vimolmangkang
Summary: The study evaluated the impact of magnetized water on the growth and secondary metabolite production of Pueraria candollei var. mirifica (PM) suspension cells. The results showed that magnetized water treatment increased biomass accumulation, altered the accumulation pattern of isoflavonoids, and enhanced antioxidant activity in the cells.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Chemistry, Applied
Gorawit Yusakul, Wipawee Juengsanguanpornsuk, Boonchoo Sritularak, Suppalak Phaisan, Thaweesak Juengwatanatrakul, Waraporn Putalun
Summary: (+)-7-O-Methylisomiroestrol (MeI), a novel chromene found in the Pueraria candollei var. mirifica (PM) root, has a higher estrogenic potency compared to isomiroestrol but lower than deoxymiroestrol and miroestrol. MeI serves as a new estrogenic biomarker for the chemical standardization of PM extract for health product development.
NATURAL PRODUCT RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Fonthip Makkliang, Wipawee Juengsanguanpornsuk, Suppalak Phaisan, Attapon Sakdamas, Waraporn Putalun, Seiichi Sakamoto, Gorawit Yusakul
Summary: The study successfully enhanced the estrogenic activity of Pueraria candollei var. mirifica (PM) extract by treating it with immobilized beta-glucosidase, demonstrating potential industrial application.
Article
Biochemical Research Methods
Natthapon Chuphol, Nuttikarn Nokkaew, Fonthip Makkliang, Worapol Sae-Foo, Suppalak Phaisan, Waraporn Putalun, Seiichi Sakamoto, Gorawit Yusakul
Summary: This study developed and validated an ICA with a monoclonal antibody exhibiting similar reactivity against Mi and Dmi. The ICA showed a LOD at 1 and 16 mu g/mL for Mi and Dmi, respectively. The cross-reactivity of the ICA with Dmi was lower than that observed with the icELISA, and the cross-reactivity of ICA against other compounds of the PM was correlated with those of icELISA.
PHYTOCHEMICAL ANALYSIS
(2023)
Article
Biochemistry & Molecular Biology
Yaowared Chulikhit, Wichitsak Sukhano, Supawadee Daodee, Waraporn Putalun, Rakvajee Wongpradit, Charinya Khamphukdee, Kaoru Umehara, Hiroshi Noguchi, Kinzo Matsumoto, Orawan Monthakantirat
Summary: The phytoestrogen-enriched plant Pueraria mirifica extract was found to improve cognitive impairment and hippocampal oxidative stress induced by ovariectomy in mice. The treatment attenuated oxidative brain damage and dysfunctions in the hippocampal antioxidant system while preventing neuroinflammation, thereby suggesting potential benefits for cognitive deficits associated with menopause/ovariectomy.
Article
Biochemical Research Methods
Wipawee Juengsanguanpornsuk, Nut Poopanee, Supaluk Krittanai, Seiichi Sakamoto, Hiroyuki Tanaka, Waraporn Putalun, Gorawit Yusakul
Summary: Two immunoaffinity separation approaches using active fragment antigen-binding (Fab) antibodies were developed to selectively separate miroestrol and deoxymiroestrol from Pueraria candollei var. mirifica. The methods were successful in purifying the compounds from the plant extract.
PHYTOCHEMICAL ANALYSIS
(2023)
Article
Pharmacology & Pharmacy
Napaphak Jaipakdee, Kanokwan Jarukamjorn, Waraporn Putalun, Ekapol Limpongsa
Summary: The study evaluated the permeation behaviors and chemical stability of miroestrol and deoxymiroestrol from Pueraria candollei var. mirifica (PM) in transdermal gels. Results indicated that hydroalcoholic gel exhibited the best permeation of miroestrol and deoxymiroestrol, with the highest skin permeability coefficient. Long-term stability study showed that miroestrol contents remained constant while deoxymiroestrol decreased significantly after 30 days of storage, regardless of conditions.
PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY
(2021)
Article
Integrative & Complementary Medicine
Wipawee Juengsanguanpornsuk, Gorawit Yusakul, Witsarut Kraithong, Waraporn Putalun
Summary: The study focused on extracting high phytoestrogen content from Pueraria candollei var. mirifica root cortex, with the most efficient extraction achieved using 10% ethanol. It was found that potent phytoestrogens and isoflavonoids were mainly distributed in the soluble extract, while less polar unidentified compounds were concentrated in the insoluble extract. The estrogenic activity of the soluble extract was confirmed through cell proliferation assays, with deoxymiroestrol identified as the key contributor to the proliferative effects of PM.
JOURNAL OF HERBAL MEDICINE
(2021)
Article
Chemistry, Analytical
Wipawee Juengsanguanpornsuk, Gorawit Yusakul, Tharita Kitisripanya, Supaluk Krittanai, Thaweesak Juengwatanatrakul, Seiichi Sakamoto, Waraporn Putalun
Summary: The developed ELISA method is effective in detecting trace amounts of methylisomiroestrol in Pueraria candollei, which is crucial for standardization of the products and improving the quality of plant-derived products.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Biotechnology & Applied Microbiology
Dolly Rani, Khwanlada Kobtrakul, Jittima Amie Luckanagul, Wuttinont Thaweesest, Pornchai Rojsitthisak, Wanchai De-Eknamkul, Sornkanok Vimolmangkang
Summary: The age of Pueraria candollei var. mirifica callus significantly affects its chemical profiles and bioactivities, with different growth stages exhibiting varied gene expression and chemical compositions. This study provides important data for further research on the biosynthetic pathway of isoflavonoids.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Plant Sciences
Chao Xi, Mingyue Zhang, Bingtao Li, Xiaowei Meng, Shangcheng Xu, Hong Du, Xinyi Wang, Jingping Xu, Hong Ke, Yanru Cui, Fei Qu
Summary: The anti-inflammatory effects of Pueraria lobata and Pueraria thomsonii have been confirmed, and their mechanism of action involves regulating arachidonic acid metabolism and glutathione metabolism. These findings provide a scientific basis for the development of new drugs for inflammation-related diseases and lay a foundation for the utilization of herbal resources such as Pueraria lobata and Pueraria thomsonii.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Chemistry, Applied
Worapol Sae-Foo, Supaluk Krittanai, Wipawee Juengsanguanpornsuk, Gorawit Yusakul, Tharita Kitisripanya, Seiichi Sakamoto, Waraporn Putalun
Summary: A study successfully established an ELISA method for quantifying deoxymiroestrol in Pueraria candollei, demonstrating accurate detection and recovery of the original content. The developed ELISA method showed consistent results with traditional ELISA method, which can be applied for quality control of deoxymiroestrol in Pueraria candollei.
FOOD AND AGRICULTURAL IMMUNOLOGY
(2021)
Article
Plant Sciences
Xufeng Xiao, Yuanfeng Hu, Ming Zhang, Shucheng Si, Haonan Zhou, Weifeng Zhu, Fei Ge, Caijun Wu, Shuying Fan
Summary: In this study, the expansion mechanism of Pueraria tuberous roots at six developmental stages was explored. The P3 stage was identified as a critical period for expansion, characterized by thickening diameter and rapid yield gain of tuberous roots, followed by longitudinal elongation. Transcriptome sequencing revealed 17,441 differentially expressed genes, with 386 genes shared across all six stages. KEGG pathway analysis showed that these shared genes were mainly involved in pathways related to cell wall and cell cycle, plant hormone signal transduction, sucrose and starch metabolism, and transcription factor regulation. Several transcription factors, such as bHLHs, AP2s, ERFs, MYBs, WRKYs, and bZIPs, were found to be involved in cell differentiation, division, and expansion, potentially contributing to tuberous root expansion. Six candidate genes involved in tuberous root expansion were identified, with CDC48, ARF, and EXP genes upregulated and INV, EXT, and XTH genes downregulated.
Article
Geriatrics & Gerontology
Patsira Warinsiriruk, Chananya Tantitham, Wichai Cherdshewasart, S. Abbas Shobeir, Jittima Manonai
Summary: This study compared the effects of 6% Pueraria mirifica vaginal gel with placebo gel on postmenopausal women. The results showed that the Pueraria mirifica group had decreased arterial resistance index, increased vaginal maturation index and vaginal health index compared to the placebo group.
Article
Biochemistry & Molecular Biology
Panitch Boonsnongcheep, Piriyakorn Pichetpongtorn, Phimphut Juntosuth, Tanwarat Yowsong, Pavinee Vansanit, Srisomporn Preeprame, Tripetch Kanchanapoom, Waraporn Putalun
Summary: Verbascoside, an antioxidant compound found in various plants, was successfully produced in vitro from Thai medicinal plants Clerodendrum indicum and Acanthus ebracteatus by culturing under blue LEDs and elicitation with methyl jasmonate. The production was significantly enhanced, with a 1.6-1.8 fold increase in verbascoside content. This study provides a sustainable and efficient alternative for verbascoside production.
JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hathairat Buraphaka, Watthika Puttha, Waraporn Putalun
Summary: Ardisia elliptica extracts demonstrated potent antioxidant and anti-inflammatory properties, with the root extract showing the highest phenolic and flavonoid content and the most effective free radical scavenging activity. The extracts also suppressed mRNA expression of inducible nitric oxide synthase, resulting in inhibition of nitric oxide production in stimulated macrophages. The bioactive compounds in AE extracts have potential applications in food and pharmaceutical industries.
CHEMISTRY & BIODIVERSITY
(2022)
Review
Chemistry, Medicinal
Poomraphie Nuntawong, Waraporn Putalun, Hiroyuki Tanaka, Satoshi Morimoto, Seiichi Sakamoto
Summary: Phytoproducts play important roles in various industries, and small-molecule organic compounds can be used to determine the quality and safety of plant samples. The lateral flow immunoassay (LFA) is a simple, portable, and rapid method for detecting and analyzing phytoproducts.
JOURNAL OF NATURAL MEDICINES
(2022)
Article
Plant Sciences
Akihiro Ochi, Makoto Yoritate, Tomofumi Miyamoto, Kazuteru Usui, Gorawit Yusakul, Waraporn Putalun, Hiroyuki Tanaka, Go Hirai, Satoshi Morimoto, Seiichi Sakamoto
Summary: Harringtonine (HT), derived from Cephalotaxus species, displays potent antiproliferative activity against myeloid leukemia cells. By replacing the C-5' methyl group with different hydrocarbon chains, the derivative 1f showed the highest cytotoxicity among the HT ester derivatives produced, exhibiting strong antiproliferative activity against HL-60 and HeLa cells.
JOURNAL OF NATURAL PRODUCTS
(2022)
Article
Biochemistry & Molecular Biology
Rattanathorn Choonong, Jiranan Chaingam, Ruttanaporn Chantakul, Sirikan Mukda, Prapapan Temkitthawon, Kornkanok Ingkaninan, Thaweesak Juengwatanatrakul, Gorawit Yusakul, Tripetch Kanchanapoom, Waraporn Putalun
Summary: This study investigated the PDE-5 inhibitory activity of Eurycoma longifolia and Eurycoma harmandiana plants, with canthin-6-one alkaloids showing strong inhibitory effects. In vitro root cultures of EH have higher concentrations of canthin-6-one alkaloids, making them a potential replacement for intact plants and a promising focus for further research and development as health supplements.
CHEMISTRY & BIODIVERSITY
(2022)
Article
Chemistry, Applied
Supaluk Krittanai, Piriyakorn Pichetpongtorn, Seiichi Sakamoto, Waraporn Putalun
Summary: Licochalcone A (LicoA), a compound found in Chinese licorice, has been approved for the treatment of rosacea and acne due to its antimicrobial and anti-inflammatory properties. A monoclonal antibody against LicoA was developed using a mannich reaction hapten conjugate and hybridoma technique. An ELISA method was then developed to quantitatively determine the concentration of LicoA. This method showed high specificity and can be used for quality control of licorice and its derived products.
FOOD AND AGRICULTURAL IMMUNOLOGY
(2022)
Article
Chemistry, Applied
Jiranan Chaingam, Rattanathorn Choonong, Thaweesak Juengwatanatrakul, Tripetch Kanchanapoom, Waraporn Putalun, Gorawit Yusakul
Summary: The anti-inflammatory effects of root and callus cultures of Eurycoma longifolia Jack (EL) and Eurycoma harmandiana Pierre (EH) on inflammatory cells were evaluated in this study. The tissue cultures produced compounds that exhibited anti-inflammatory effects, suggesting they could be a valuable and sustainable source of active ingredients.
FOOD AND AGRICULTURAL IMMUNOLOGY
(2022)
Article
Chemistry, Applied
Warangkana Traiyasuk, Jukrapun Komaikul, Natthinee Anantachoke, Tharita Kitisripanya
Summary: This study aims to establish the in vitro culture of A. mahidolae and investigate the effects of elicitors on their phenolic and flavonoid production. The findings demonstrate that cell suspension cultures of A. mahidolae treated with salicylic acid or yeast extract exhibit significantly higher levels of phenolic and flavonoid content and antioxidant activities.
NATURAL PRODUCT RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Worapol Sae-Foo, Gorawit Yusakul, Tharita Kitisripanya, Poomraphie Nuntawong, Seiichi Sakamoto, Waraporn Putalun
Summary: In this study, recombinant Fab antibodies against miroestrol were developed and applied for the quality control of Pueraria candollei. The Fab antibodies showed higher stability and better detection ability compared to ScFv antibodies.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemical Research Methods
Natthapon Chuphol, Nuttikarn Nokkaew, Fonthip Makkliang, Worapol Sae-Foo, Suppalak Phaisan, Waraporn Putalun, Seiichi Sakamoto, Gorawit Yusakul
Summary: This study developed and validated an ICA with a monoclonal antibody exhibiting similar reactivity against Mi and Dmi. The ICA showed a LOD at 1 and 16 mu g/mL for Mi and Dmi, respectively. The cross-reactivity of the ICA with Dmi was lower than that observed with the icELISA, and the cross-reactivity of ICA against other compounds of the PM was correlated with those of icELISA.
PHYTOCHEMICAL ANALYSIS
(2023)
Article
Biochemical Research Methods
Supaluk Krittanai, Rattanathorn Choonong, Khunkhang Butdapheng, Jakkrit Jabsanthia, Seiichi Sakamoto, Waraporn Putalun
Summary: This study aimed to develop an enzyme-linked immunosorbent assay (ELISA) using a glabridin-specific antibody. The glabridin-bovine serum albumin conjugates were injected into mice to produce hybridomas, and a highly specific antibody against glabridin was produced. The developed ELISA method showed high sensitivity and specificity for quantifying glabridin in plant materials and human serum samples.
PHYTOCHEMICAL ANALYSIS
(2023)
Article
Plant Sciences
Jukrapun Komaikul, Sasiporn Ruangdachsuwan, Duangnapa Wanlayaporn, Somnuek Palabodeewat, Surat Punyahathaikul, Theeraporn Churod, Rattanathorn Choonong, Tharita Kitisripanya
Summary: This study evaluated the anti-HCoV-OC43 effect of andrographolide and A. paniculata extracts, and the correlation between andrographolide concentration and the anti-HCoV-OC43 activity. The results showed that both andrographolide and A. paniculata extracts exhibited antiviral activity against HCoV-OC43, but the antiviral activity of the extracts did not have a linear relationship with andrographolide concentration.
Article
Materials Science, Multidisciplinary
M. Jariyaboon, P. Masrinoul, J. Komaikul, K. Muenkaew, S. Juntarapornchai, K. Ketsuwan, E. Rodpai, S. Ruangdachsuwan, S. Palabodeewat, C. Chitichotpanya
Summary: Self-virucidal Ag-TiO2 nanocomposites at low toxicity dose have the potential to reduce the risks of SARS-CoV-2 infection and excessive disinfectant usage. The nanocomposites show high virucidal efficacy at high doses, damaging the viral genome within 1 minute, and significant reduction of viral infectivity and cytotoxicity at low doses. The results suggest that Ag-TiO2 nanocomposites can be a more eco-friendly and safer disinfectant option with broad spectrum action against SARS-CoV-2 variants.
EMERGENT MATERIALS
(2023)
Correction
Chemistry, Multidisciplinary
Attapon Sakdamas, Fonthip Makkliang, Waraporn Putalun, Thaweesak Juengwatanatrakul, Tripetch Kanchanapoom, Seiichi Sakamoto, Gorawit Yusakul
Article
Chemistry, Multidisciplinary
Attapon Sakdamas, Fonthip Makliang, Waraporn Putalun, Thaweesak Juengwatanatrakul, Tripetch Kanchanapoom, Seiichi Sakamoto, Gorawit Yusakul
Summary: Micellar liquid chromatography (MLC) was used to analyze low concentration canthin-6-one alkaloids in extracts of Eurycoma longifolia Jack (EL) and Eurycoma harmandiana Pierre (EH). The MLC method showed comparable sensitivity, precision, accuracy, and acetonitrile consumption to the conventional high-performance liquid chromatography (HPLC) method. However, the HPLC method exhibited better performance for samples with low analyte concentrations and varying sample matrices, especially EL and EH samples. Overall, the MLC analysis of canthin-6-one alkaloids was limited to trace analytes due to interference by the matrix.
Article
Chemistry, Analytical
Bingbing Liu, Jie Cao, Lu Liu, Min Zeng, Hongli Yu, Hao Wu
Summary: This study investigated the effect of processing with Chebulae Fructus on the chemical composition of Euphorbia pekinensis (EP) and found that it could alter the composition and reduce toxicity, providing a scientific basis for optimizing processing technology and handling other ethnomedicines with toxic properties.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Renuka P. Rathnasekara, Abu M. Rustum
Summary: A simple and robust HPLC method was developed for simultaneous determination of four active pharmaceutical ingredients and their degradation products. The method successfully separated the peaks of interest and demonstrated stability-indicating capability by separating the degradation products in stressed samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A. Fox, Patricia Ellison, Robert O. Ryan, Paul J. Brett, David Aucoin, Xiaoshan Zhu
Summary: In this study, a new type of Mn-doped nanocrystals was developed for detecting a biomarker of a fatal disease. The sensitivity of the detection was enhanced by surface treatment and time-resolved reading, achieving a detection limit comparable to enzyme-based immunoassay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xiaoling Liu, Ting Yang, Lu Chen, Lili Lan, Guoxiang Sun, Ping Guo
Summary: In this study, the quality of Yiqing tablet was comprehensively evaluated using multi-component quantification, multi-dimensional fingerprint construction, and antioxidant activity analysis. The results showed that the sample had 25 components with antioxidant activity according to the HPLC fingerprint and most bands of FTIR also showed antioxidant activity. Based on the evaluation results of HPLC and FTIR fingerprint, all samples were classified as first level, demonstrating the consistency of sample quality. This study provides a new idea for achieving comprehensive quality evaluation of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Siqi Li, Hao Guo, Yan Gao, Wen Tian, Shan Wang, Chen Shen, Lili Xu, Hailong Liu, Jinliang Zhang, Yingwu Wang
Summary: Using competitive ELISA to detect free cytokines is limited in accurately determining their real state and quantity. In this study, a novel cytokine immunoassay was developed using Rosetta molecular docking prediction technique to establish a method that can detect the true concentration of free cytokines without disrupting the dynamic equilibrium.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Changqing Yu, Tiantian Xie, Haiyan Liu, Ligai Bai
Summary: A phenyl-hybrid monolithic adsorbent with porous structure and multiple functional groups was prepared. It was used for the online extraction and quantification of coumarins in mouse plasma, showing good removal ability and selectivity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Joao B. Pereira Jr, Vanessa S. Carvalho, William Q. Ferreira, Rennan G. O. Araujo, Sergio L. C. Ferreira
Summary: This work presents a simple, cost-effective, and environmentally friendly method for determining the content of multiple elements in medicinal herbs using ICP OES. The optimized experimental parameters result in low dissolved organic carbon content and residual acidity concentration. The accuracy of the method is confirmed through the analysis of certified reference materials, and its environmental friendliness is validated using the AGREE metric. Principal component analysis is used to classify the medicinal herbs based on their inorganic constituent concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Orwa Siddig, Chenxi Liu, Mohamed Abdulbagi, Min Song, Yu-ting Lu, Tai-jun Hang
Summary: In this study, a reliable LC-QTOF-MS method was developed to separate and characterize process-related substances and degradation products of Lurasidone hydrochloride. The results showed that Lurasidone hydrochloride exhibited instability under certain stress conditions, and several previously unidentified compounds were discovered. The study emphasizes the importance of monitoring and controlling related substances in the manufacturing processes to optimize the process and ensure quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Jiaxi Fang, Luping Wang, Di Zhang, Yan Liang, Shouxin Li, Jingkui Tian, Qiang He, Juan Jin, Wei Zhu
Summary: This study reports that a novel compound called CPS-A exhibits therapeutic effects on cisplatin-induced acute kidney injury (AKI) and explores its potential mechanisms through transcriptomics and metabolomics. CPS-A improves cell viability, reduces inflammatory markers, and attenuates cisplatin-induced kidney injury. The study also identifies potential targets related to amino acid metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ricardo Conde, Nadia Oliveira, Elisabete Morais, Ana Paula Amaral, Adriana Sousa, Goncalo Graca, Ignacio Verde
Summary: Decreased levels of tyrosine, glutamate, valine, leucine, and isoleucine are associated with worsening pathological cognitive decline. Decreased levels of isobutyrate, histidine, acetone, and unknown-1 metabolite are potential biomarkers with neuroprotective and antioxidant actions for cognitive decline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
A. L. Santunione, F. Palazzoli, P. Verri, D. Vandelli, V. Castagnetti, C. Profeta, E. Silingardi
Summary: This study describes two fatal suicide cases involving four cardiovascular drugs, and presents the concentrations and distribution of these drugs in different post-mortem specimens. The study developed a fast, sensitive, and simple method for drug detection suitable for forensic purposes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Meiyun Shi, Xinyue Zheng, Yuncheng Ge, Ning Zhang, Luyao Yu, Xujian Duan, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin
Summary: This study investigated the cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in MCF-7 cells using UPLC-MS/MS and CID techniques. The results showed that PEG600, PEG2K, PEG4K and PEG10K exhibited low toxicity and had limited cellular entry at concentrations of 5-1200 μg/mL. Additionally, the environmental friendliness of the developed analytical methods was evaluated.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ruijin Geng, Mengjie Yu, Jinbiao Xu, Yuanwang Wei, Qiong Wang, Junguo Chen, Fei Sun, Kun Xu, Han Xu, Xiaohui Liu, Juhua Xiao, Xianchao Zhang, Baogang Xie
Summary: This study validated serum methionine as a potential biomarker for Kawasaki disease (KD), and found that its alteration is associated with the activation of DNMT1 in KD patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Eman Shawky, Rasha A. Nassra, Aliaa M. T. El-Alkamy, Shaimaa M. Sallam, Samah M. El Sohafy
Summary: This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders and the potential therapeutic effects of fenugreek constituents. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. Animal model study further supported the presence of these bioactive compounds and their effects on body weight, serum prolactin levels, serum estradiol levels, and ovarian histological structure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xuelong Zhao, Ruixue Dai, Jing Wang, Liangliang Cao, Peidong Chen, Weifeng Yao, Fangfang Cheng, Beihua Bao, Li Zhang
Summary: This study identified the skin-permeable and skin-retainable components of Cayratia japonica ointment and found that flavonoids are the main active components that play a crucial role in the inflammatory and proliferative phases of wound healing.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
