Article
Cardiac & Cardiovascular Systems
Pia Sjoberg, Petru Liuba, Hakan Arheden, Einar Heiberg, Marcus Carlsson
Summary: This study compared hemodynamic parameters obtained from PV loops in Fontan circulation patients with controls, revealing significant differences in certain parameters.
BMC CARDIOVASCULAR DISORDERS
(2022)
Article
Cardiac & Cardiovascular Systems
Manuel J. Richter, Athiththan Yogeswaran, Faeq Husain-Syed, Istvan Vadasz, Zvonimir Rako, Emad Mohajerani, Hossein A. Ghofrani, Robert Naeije, Werner Seeger, Ulrike Herberg, Andreas Rieth, Ryan J. Tedford, Friedrich Grimminger, Henning Gall, Khodr Tello
Summary: The study assessed the feasibility of constructing RV pressure-volume loops solely by echocardiography, showing promising results in terms of correlation between echocardiographic and conductance-derived PV loop parameters. Further validation is warranted to support the use of this novel echocardiographic method in assessing RV contractility and myocardial work.
EUROPEAN HEART JOURNAL-CARDIOVASCULAR IMAGING
(2022)
Article
Cardiac & Cardiovascular Systems
Lukas Opatril, Roman Panovsky, Jan Machal, Tomas Holecek, Lucia Masarova, Vera Feitova, Vladimir Kincl, Marek Hodejovsky, Lenka Spinarova
Summary: In this study, a synthetic haematocrit formula was generated based on native relaxation time values and the reciprocal model was found to be the most precise in estimating haematocrit. Adding post-contrast values to the calculation significantly improved the precision of the formula.
BMC CARDIOVASCULAR DISORDERS
(2021)
Article
Geriatrics & Gerontology
Serena Marcozzi, Giorgia Bigossi, Maria Elisa Giuliani, Robertina Giacconi, Maurizio Cardelli, Francesco Piacenza, Fiorenza Orlando, Agnese Segala, Alessandra Valerio, Enzo Nisoli, Dario Brunetti, Annibale Puca, Federico Boschi, Carlo Gaetano, Alessia Mongelli, Fabrizia Lattanzio, Mauro Provinciali, Marco Malavolta
Summary: Frailty is an age-related condition characterized by functional decline, vulnerability to stressors, and adverse health outcomes. Two validated measurements, frailty phenotype (FP) and clinical frailty index (CFI), have limitations in identifying frail mice. In this study, a physical function score (PFS) was developed as a continuous variable integrating the criteria of FP to reduce misclassification of frailty. The PFS, along with CFI, was used to calculate a vitality score (VS) in aging mice, which showed higher association with mortality and correlated with biomarkers linked to senescent cells and the epigenetic clock. This non-invasive assessment and the VS may improve frailty identification, reduce sample size, and evaluate interventions for age-related diseases in geriatric animals.
Article
Multidisciplinary Sciences
Sam D. Hutchings, Jim Watchorn, Rory McDonald, Su Jeffreys, Mark Bates, Sarah Watts, Emrys Kirkman
Summary: Comparing the stroke volume measured by TEB and SSD devices against the reference standard of TTE, the study found potential measurement errors in detecting hypovolaemia, cautioning against their interpretation in clinical practice.
Article
Physiology
Pia Sjoberg, Felicia Seemann, Hakan Arheden, Einar Heiberg
Summary: The study improved and validated a method for non-invasive analysis of PV loops, providing a more accurate diagnostic tool for cardiovascular diseases in a wider range of study cohorts, not only in resting state but also under stress conditions.
CLINICAL PHYSIOLOGY AND FUNCTIONAL IMAGING
(2021)
Article
Physiology
Tsung-Yu Ko, Chia-Chuan Chuang, Mao-Shin Lin, Yi-Chang Chen, Ying-Hsien Chen, Ching-Chang Huang, Chih-Fan Yeh, Ming-Jiuh Wang, Kuo-Chu Chang, Yi-Lwun Ho, Hsien-Li Kao
Summary: This study proposed a new minimally invasive method for pressure-volume analysis in patients with severe aortic stenosis receiving transcatheter aortic valve implantation (TAVI). The results showed that ventriculoarterial coupling and left ventricle mechanics improved immediately after TAVI.
PHYSIOLOGICAL REPORTS
(2023)
Article
Cell Biology
Byung Ho Lee, Irene Seijo-Barandiaran, Anne Grapin-Botton
Summary: Epithelial organoids can mimic morphogenetic processes in vivo, such as lumen and multilayer formation, folding, branching, delamination, and elongation. This article elaborates on the signaling molecules controlling morphogenesis, including their emergence as signaling centers in the organoids. It also discusses how mechanical cues and environmental material properties influence organoid shape.
CURRENT OPINION IN GENETICS & DEVELOPMENT
(2022)
Article
Optics
Julien Gasser, Daryl Warpelin, Felix Bussieres, Jerome Extermann, Enrico Pomarico
Summary: We present a Raman distributed temperature sensor based on standard telecom single mode fibers and efficient polarization-independent superconducting nanowire single photon detectors. The sensor achieves a resolution of 3 cm and 1.5 degrees C on a 5 m fiber, and the temperature resolution is affected by the drop in laser repetition rate for long fibers. With 3 minutes integration on a 500 m fiber, a trade-off of 10 cm and 8 degrees C resolution is achieved.
Editorial Material
Cell & Tissue Engineering
Anne Grapin-Botton, Barbara Ludwig
Summary: Du et al. conducted the first transplantation of 0 cells derived from pluripotent stem cells in diabetic monkeys, as a step towards clinical translation. They observed gradual benefits over months, but also noted immune rejection of the grafts at 5-6 months.
Article
Optics
Yan Liu, Jonathan Dong, Cedric Schmidt, Aleix Boquet-Pujadas, Jerome Extermann, Michael Unser
Summary: Optical projection tomography (OPT) is a powerful tool for 3D imaging of mesoscopic samples. This study focuses on addressing the issue of refractive-index mismatch and proposes a fast and efficient reconstruction method to correct the induced artifacts.
Review
Cell Biology
Tzer Han Tan, Jifeng Liu, Anne Grapin-Botton
Summary: The functional relevance of an organoid depends on its differentiation, morphology, cell arrangement, and biophysical properties, which collectively define its state. Transcriptomics and high-content image analysis are the most effective methods for characterizing the state variables of an organoid or the cells that compose it, and comparing them to their in vivo counterparts. Organoids explore a wider state space than in vivo organs due to the lack of niche signaling and the variability of boundary conditions in vitro. By using data-driven state inference and in silico modeling, phase diagrams can be constructed to systematically sort organoids based on biochemical or biophysical axes, providing strategies for modulating their state by manipulating the biochemical and biophysical environment and the cells used for seeding.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Enrico Pomarico, Cedric Schmidt, Florian Chays, David Nguyen, Arielle Planchette, Audrey Tissot, Adrien Roux, Stephane Pages, Laura Batti, Christoph Clausen, Theo Lasser, Aleksandra Radenovic, Bruno Sanguinetti, Jerome Extermann
Summary: The extensive use of supervised learning models on compressed datasets has become possible due to the growth of data throughput in optical microscopy. This study quantifies the statistical distortions induced by compression and compares them to the raw predictive uncertainty. The results show that image compression can significantly alter predictions and that higher compression ratios lead to larger distortions.
SCIENTIFIC REPORTS
(2022)
Article
Cell & Tissue Engineering
Akiko Nakamura, Yan Fung Wong, Andrea Venturato, Magali Michaut, Seshasailam Venkateswaran, Mithun Santra, Carla Goncalves, Michael Larsen, Marit Leuschner, Yung Hae Kim, Joshua Brickman, Mark Bradley, Anne Grapin-Botton
Summary: In this study, a protocol for long-term expansion of human pancreatic progenitors in a defined medium was developed, and a polymer that can replace fibronectin was identified. The expanded progenitors showed more efficient differentiation into glucose-responsive 13 cells and produced fewer glucagon-expressing cells compared to directly differentiated progenitors. This method provides flexibility in research and therapeutic production by allowing the expansion and cryopreservation of progenitors under defined conditions.
Review
Cell Biology
Rashmiparvathi Keshara, Yung Hae Kim, Anne Grapin-Botton
Summary: Organoids are simplified and miniaturized versions of organs produced in vitro from stem or progenitor cells. They are useful for studying development, homeostasis, regeneration, and disease. Imaging methods play a pivotal role in visualizing and understanding organoids' self-organization, growth, differentiation, and function.
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Developmental Biology
Anne Grapin-Botton, Yung Hae Kim
Summary: Organoids have become a rapidly advancing and widely used model in biological and medical research. This review focuses on the development of pancreas organoids in vitro, covering methods from embryonic, fetal, adult cells, to pluripotent stem cells. The review discusses how these systems have enhanced our understanding of pancreas development, regeneration, and diseases, as well as their limitations and potential for future discoveries.
Review
Endocrinology & Metabolism
Belin Selcen Beydag-Tasoez, Siham Yennek, Anne Grapin-Botton
Summary: This Review assesses the progress in developing pancreatic organoids and bioengineered systems for modeling diabetes mellitus and its complications. The merger between the organoid and bioengineering fields will provide integrative models for studying the multi-organ disease.
NATURE REVIEWS ENDOCRINOLOGY
(2023)
Article
Neurosciences
Celine Loussert-Fonta, Luc Stoppini, Yoan Neuenschwander, Ophelie Righini, Denis Prim, Cedric Schmidt, Marc O. Heuschkel, Loris Gomez Baisac, Milica Jovic, Marc E. Pfeifer, Jerome Extermann, Adrien Roux
Summary: Traumatic brain injury (TBI) is a complex condition with a wide range of pathophysiological changes. Animal models have been used to study the relationship between mechanical damage and neural cell functions, but they have limitations in reproducing human brain trauma. Researchers have therefore developed an in vitro platform using human iPS cells to induce brain injuries, allowing for better understanding of the biological mechanisms involved. By measuring electrophysiological activities, quantifying biomarker release, and using imaging methods, researchers have observed significant changes in tissue function and cell death resulting from TBI.
FRONTIERS IN NEUROSCIENCE
(2023)
Article
Biology
Arielle Louise Planchette, Cedric Schmidt, Olivier Burri, Mercedes Gomez de Agueero, Aleksandra Radenovic, Alessio Mylonas, Jerome Extermann
Summary: Optical projection tomography (OPT) enables the observation and understanding of tissue-wide networks in three dimensions. A multi-modal workflow for characterizing the mouse small intestine is presented, demonstrating its applicability for imaging the intestinal immune compartment and mucosal structures.
COMMUNICATIONS BIOLOGY
(2023)
Article
Cell Biology
Belin Selcen Beydag-Tasoez, Joyson Verner D'Costa, Lena Hersemann, Byung Ho Lee, Federica Luppino, Yung Hae Kim, Christoph Zechner, Anne Grapin-Botton
Summary: This study investigates the dynamics of NEUROG3 gene expression in human pancreatic development. The researchers find that human NEUROG3 expression is approximately 2-fold slower than that of mice. They also observe heterogeneous peak levels of NEUROG3 expression and demonstrate that both low and high peak levels can trigger the differentiation of cells into hormone-expressing cells. By integrating single-cell transcriptome data with live cell imaging, they propose a data-mapping methodology applicable to other contexts and identify a role for KLK12 in motility at the onset of NEUROG3 expression.
DEVELOPMENTAL CELL
(2023)
Review
Biochemistry & Molecular Biology
Jay Gopalakrishnan, Kerstin Feistel, Benjamin M. Friedrich, Anne Grapin-Botton, Nathalie Jurisch-Yaksi, Elvira Mass, David U. Mick, Roman-Ulrich Mueller, Helen May-Simera, Bernhard Schermer, Miriam Schmidts, Peter Walentek, Dagmar Wachten
Summary: Primary cilia are dynamic cellular protrusions that play a crucial role in sensing and processing extracellular signals, controlling cell fate, and function during tissue development.
Article
Cell & Tissue Engineering
Zahra Ghezelayagh, Mahsa Zabihi, Ibrahim Zarkesh, Carla A. C. Goncalves, Michael Larsen, Newsha Hagh-parast, Mohammad Pakzad, Massoud Vosough, Babak Arjmand, Hossein Baharvand, Bagher Larijani, Anne Grapin-Botton, Hamid Reza Aghayan, Yaser Tahamtani
Summary: In this study, the impact of co-culturing human embryonic stem cell-derived pancreatic progenitors with human fetal pancreatic-derived mesenchymal cells on endocrine and beta cell development was evaluated. It was found that pancreatic mesenchyme had an inductive effect on pancreatic progenitors, promoting beta cell maturation. Additionally, scalable cultures combining these cells were investigated for their potential applications in mimicking pancreatic tissue for developmental studies.
STEM CELL REVIEWS AND REPORTS
(2022)