Article
Multidisciplinary Sciences
Veronica Gatti, Claudia Fierro, Mirco Compagnone, Veronica La Banca, Alessandro Mauriello, Manuela Montanaro, Stefano Scalera, Francesca De Nicola, Eleonora Candi, Francesco Ricci, Luca Fania, Gerry Melino, Angelo Peschiaroli
Summary: Delta Np63, a master regulator of epithelial biology, interacts with Senataxin (SETX) to sustain the expression of epidermal differentiation genes and regulate the differentiation of keratinocytes. SETX physically binds the p63 DNA-binding motifs present in early epidermal differentiation genes KRT1 and ZNF750, facilitating R-loop removal and promoting gene expression.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Marco Napoli, Sarah J. Wu, Bethanie L. Gore, Hussein Abbas, Kyubum Lee, Rahul Checker, Shilpa Dhar, Kimal Rajapakshe, Aik Choon Tan, Min Gyu Lee, Cristian Coarfa, Elsa R. Flores
Summary: Distinct lung stem cells regulated by increment Np63 play a role in the development of both lung adenocarcinoma and squamous cell carcinoma through the regulation of a common landscape of enhancer-associated genes, including BCL9L.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
K. Venkatesan Iyer, Anna Taubenberger, Salma Ahmed Zeidan, Natalie A. Dye, Suzanne Eaton, Frank Juelicher
Summary: The study found that in epithelial tissues, the levels of Lamin A/C change with apico-basal cell compression independently of tissue stiffness. Nuclear deformation and phosphorylation levels of Lamin A/C are key factors in regulating Lamin A/C levels.
NATURE COMMUNICATIONS
(2021)
Article
Plant Sciences
Yachen Wang, Hantai Yin, Zongxing Long, Wenjuan Zhu, Junmei Yin, Xiqiang Song, Chonghui Li
Summary: In this study, the regulatory roles of transcription factors DhMYB2 and DhbHLH1 in anthocyanin biosynthesis in Phalaenopsis-type Dendrobium were systematically investigated. The results showed that DhMYB2 and DhbHLH1 are specifically expressed in flowers with anthocyanin accumulation, and their expression patterns are similar to those of genes in the anthocyanin biosynthesis pathway. The study also revealed that DhMYB2 and DhbHLH1 can bind to the promoter regions of structural genes, indicating their role as transcriptional activators.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Tsubasa Shoji, Kazuki Saito
Summary: This study provides insights into the molecular mechanism of SGA biosynthesis and its regulation in eggplant, offering a theoretical basis for further understanding of the anti-nutritional defense in eggplant plants.
Article
Chemistry, Multidisciplinary
William W. Du, Javeria Qadir, Kevin Y. Du, Yu Chen, Nan Wu, Burton B. Yang
Summary: Current studies have shown that nuclear actin plays a significant role in the regulation of epithelial-mesenchymal transition (EMT). Different binding partners for nuclear F-actin and G-actin have been discovered, which respectively modulate EMT-promoting and EMT-repressing transcriptional events. Mechanistically, nuclear F-actin enhances the expression and stability of proteins involved in EMT promotion, while nuclear G-actin increases the expression and stability of proteins involved in EMT repression. The association between nuclear actin and EMT suggests that targeting nuclear actin dynamics for dysregulated EMT is a promising area for further study.
Article
Dermatology
Subhashree Nayak, Kan Jiang, Emma Hope, Michael Cross, Andrew Overmiller, Faiza Naz, Stephen Worrell, Deepti Bajpai, Kowser Hasneen, Stephen R. Brooks, Stefania Dell'Orso, Maria I. Morasso
Summary: Chromatin landscape and regulatory networks play crucial roles in lineage specification and differentiation. This study explored the temporal and spatial differentiation axis in murine epidermal cells and revealed the regulatory role of the Dlx/Klf/Grhl network and superenhancers in epidermal differentiation.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2023)
Review
Plant Sciences
Chieko Goto, Ikuko Hara-Nishimura, Kentaro Tamura
Summary: The shape of plant nuclei varies among different species, tissues, and cell types, regulated by several nuclear envelope proteins. These proteins not only influence nuclear shape but also participate in nuclear and cellular functions such as maintenance of chromatin structure, gene expression, and plant growth.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biology
Emanuele Panatta, Alessio Butera, Ivana Celardo, Marcel Leist, Gerry Melino, Ivano Amelio
Summary: Nuclear organization and architecture are crucial for genomic integrity, epigenetic regulation, and gene expression. This study reveals that loss of p53 is associated with increased expression of nuclear pore complex and nuclear lamina members, and directly regulates the transcription of Lamin B1.
Article
Biology
Tetsuya Yamamoto, Takahiro Asanuma, Yota Murakami
Summary: In this study, a kinetic model was used to evaluate the relationship between heterochromatin assembly, small RNA production, and histone methylation in fission yeast. The theory of heterochromatin assembly was proposed by considering the connectivity of tandemly repeated genes along the chromatin in the kinetic equations of small RNA production and histone methylation. The theory predicts the steady production of small RNAs due to the polymeric nature of tandemly repeated genes and the compaction of these genes suppresses the production of small RNAs, which is consistent with recent experiments. This theory can be applied to small RNA-dependent gene silencing in higher organisms.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Paola Fortugno, Rosanna Monetta, Manuel Belli, Elisabetta Botti, Francesco Angelucci, Maria Grazia Palmerini, Nottola Stefania Annarita, Chiara De Luca, Marina Ceccarini, Marco Salvatore, Luca Bianchi, Pompeo Macioce, Massimo Teson, Francesco Ricci, Italian Undiagnosed Diseases Network, Guido Macchiarelli, Biagio Didona, Antonio Costanzo, Daniele Castiglia, Francesco Brancati
Summary: This study identified two novel RIPK4 gene mutations associated with ED and observed clinical overlap with ED caused by mutations in transcription factors and adhesion molecules. Mutations in the RIPK4 gene result in problems in epithelial cell differentiation and cell adhesion.
HUMAN MOLECULAR GENETICS
(2022)
Review
Cell Biology
Dana L. Woodstock, Morgan A. Sammons, Martin Fischer
Summary: p53 and p63, as transcription factors, regulate both shared and unique target genes to exert their tumor suppressive or oncogenic effects. The previous notion of strict competition between the two siblings needs to be reevaluated, as they can also collaborate towards a common goal.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Engineering, Biomedical
Jian Sun, Junwei Chen, Kshitij Amar, Yanyan Wu, Mingxing Jiang, Ning Wang
Summary: There is increasing evidence that force affects cells and tissues in various physiological and pathological processes, including gene regulation. However, the molecular pathway of force transmission from the nuclear lamina to the chromatin remains unclear. This study reveals that the nuclear protein LAP2 beta mediates force transmission from the nuclear lamina to the chromatin, and this pathway plays a crucial role in gene regulation.
ACTA BIOMATERIALIA
(2023)
Article
Plant Sciences
Jie Guo, Hanxiao Liu, Keli Dai, Xiangyang Yuan, Pingyi Guo, Weiping Shi, Meixue Zhou
Summary: B3 transcription factors play important roles in plant development and growth. We identified 69 B3 transcription factors in Brachypodium distachyon and characterized their sequence characteristics and expression profiles. Our study provides insights into the functions of B3 genes.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Yanchong Yu, Yanan Qi, Jinpeng Xu, Xuehuan Dai, Jiacai Chen, Chun-Hai Dong, Fengning Xiang
Summary: The study has demonstrated that WRKY71 acts as a positive regulator of leaf senescence in Arabidopsis, directly regulating senescence-associated genes and mediating multiple signals to accelerate leaf senescence. Additionally, WRKY71 was found to mediate ethylene signaling and synthesis, providing new insights into the molecular mechanisms of leaf senescence regulation.
Article
Biology
Katarzyna Murat, Bjoern Gruening, Paulina Wiktoria Poterlowicz, Gillian Westgate, Desmond J. Tobin, Krzysztof Poterlowicz
Article
Critical Care Medicine
Kirsty Smith, Krzystof Poterlowicz, Stephen Southern, Ajay L. Mahajan, Sharmila Jivan
Summary: The accuracy of measuring burn size on a static image was assessed with the inclusion of a standard object, resulting in an overall overestimation of burn size. Despite using a standard object, small areas of the forearm were better estimated, while the back areas were most overestimated.
Article
Dermatology
Serena Buscone, Andrei N. Mardaryev, Gillian E. Westgate, Natallia E. Uzunbajakava, Natalia V. Botchkareva
Summary: The study demonstrates that 453 nm blue light induces the accumulation of CRY1 in human keratinocytes and hair follicles. CRY1 plays a role in sustaining human hair growth by affecting the expression of genes related to apoptosis and proliferation.
EXPERIMENTAL DERMATOLOGY
(2021)
Letter
Dermatology
Andrei Mardaryev, Max van Lessen, Majid Alam, Francisco Jimenez Acosta, Tamas Biro, Ralf Paus
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2021)
Article
Cell Biology
Eve Hunter-Featherstone, Natalie Young, Kathryn Chamberlain, Pablo Cubillas, Ben Hulette, Xingtao Wei, Jay P. Tiesman, Charles C. Bascom, Adam M. Benham, Martin W. Goldberg, Gabriele Saretzki, Iakowos Karakesisoglou
Summary: The study demonstrates that extracellular mechanics have significant effects on keratinocyte cell biology and should be utilized in skin research to ensure successful production of physiologically relevant skin models.
Article
Genetics & Heredity
Natalie Young, Maria Asif, Matthew Jackson, Daniel Martin Fernandez-Mayoralas, Mar Jimenez de la Pena, Beatriz Calleja-Perez, Sara Alvarez, Eve Hunter-Featherstone, Angelika A. Noegel, Wolfgang Hohne, Peter Nuernberg, Boguslaw Obara, Muhammad Sajid Hussain, Iakowos Karakesisoglou, Alberto Fernandez-Jaen
Summary: The study identified novel variations in SYNE2 in patients with autism spectrum disorder, potentially leading to intellectual disability and ASD. These variants are located in important structural domains of nesprin-2 giant protein, showing significant effects at the protein level, demonstrating the crucial role of nesprin-2.
Article
Biochemistry & Molecular Biology
Alexander E. Ostrovsky, Alexandru Mahmoud, Andrew J. Lonie, Anna Syme, Anne Fouilloux, Anthony Bretaudeau, Anton Nekrutenko, Anup Kumar, Arthur C. Eschenlauer, Assunta D. DeSanto, Aysam Guerler, Beatriz Serrano-Solano, Berenice Batut, Bjoern A. Gruening, Bradley W. Langhorst, Bridget Carr, Bryan A. Raubenolt, Cameron J. Hyde, Catherine J. Bromhead, Christopher B. Barnett, Coline Royaux, Cristobal Gallardo, Daniel Blankenberg, Daniel J. Fornika, Dannon Baker, Dave Bouvier, Dave Clements, David A. de Lima Morais, David Lopez Tabernero, Delphine Lariviere, Engy Nasr, Enis Afgan, Federico Zambelli, Florian Heyl, Fotis Psomopoulos, Frederik Coppens, Gareth R. Price, Gianmauro Cuccuru, Gildas Le Corguille, Greg Von Kuster, Gulsum Gudukbay Akbulut, Helena Rasche, Hotz Hans-Rudolf, Ignacio Eguinoa, Igor Makunin, Isuru J. Ranawaka, James P. Taylor, Jayadev Joshi, Jennifer Hillman-Jackson, Jeremy Goecks, John M. Chilton, Kaivan Kamali, Keith Suderman, Krzysztof Poterlowicz, Le Bras Yvan, Lucille Lopez-Delisle, Luke Sargent, Madeline E. Bassetti, Marco Antonio Tangaro, Marius van den Beek, Martin Cech, Matthias Bernt, Matthias Fahrner, Mehmet Tekman, Melanie C. Foell, Michael C. Schatz, Michael R. Crusoe, Miguel Roncoroni, Natalie Kucher, Nate Coraor, Nicholas Stoler, Nick Rhodes, Nicola Soranzo, Niko Pinter, Nuwan A. Goonasekera, Pablo A. Moreno, Pavankumar Videm, Petera Melanie, Pietro Mandreoli, Pratik D. Jagtap, Qiang Gu, Ralf J. M. Weber, Ross Lazarus, Ruben H. P. Vorderman, Saskia Hiltemann, Sergey Golitsynskiy, Shilpa Garg, Simon A. Bray, Simon L. Gladman, Simone Leo, Subina P. Mehta, Timothy J. Griffin, Vahid Jalili, Vandenbrouck Yves, Victor Wen, Vijay K. Nagampalli, Wendi A. Bacon, Willem de Koning, Wolfgang Maier, Peter J. Briggs
Summary: Galaxy is a mature and browser accessible workbench for scientific computing, allowing scientists to easily share, analyze, and visualize their data. It has a strong global community and support from national infrastructure providers. Key technical developments of Galaxy include improved user interface, interactive tools for data analysis, and a complete suite of machine learning tools. Important scientific developments enabled by Galaxy include Vertebrate Genome Project (VGP) assembly workflows and global SARS-CoV-2 collaborations.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Multidisciplinary Sciences
Guo-Dong Chen, Iqra Fatima, Qin Xu, Elena Rozhkova, Michael Y. Fessing, Andrei N. Mardaryev, Andrey A. Sharov, Guo-Liang Xu, Vladimir A. Botchkarev
Summary: Loss of Tet2 and Tet3 in skin epithelial cells causes significant changes in hair shape and length, leading to hair loss. Tet2 and Tet3 control the chromatin accessibility and promoter activity of hair shape-related genes (Krt25 and Krt28) through DNA demethylation, as well as the interactions between the Krt28 gene promoter and distal enhancer. In addition, Tet2 and Tet3 also regulate three-dimensional chromatin topology in Keratin type I/II gene loci via DNA methylation-independent mechanisms. These findings highlight the crucial roles of Tet2 and Tet3 in lineage-specific gene expression and hair growth control, and suggest DNA methylation modulation as a potential novel approach for hair growth control.
Article
Dermatology
Max van Lessen, Andrei Mardaryev, David Broadley, Marta Bertolini, Janin Edelkamp, Maximilian Kueckelhaus, Wolfgang Funk, Tamas Biro, Ralf Paus
Summary: In this pilot study, scientists reported that healthy human skin of different age groups undergoes extremely accelerated ageing within only 3 days when organ-cultured in a defined serum-free medium. They found significant changes in ageing-associated biomarkers in the ex vivo phenotype and discovered that caffeine and 2,5-dimethylpyrazine have anti-ageing effects.
EXPERIMENTAL DERMATOLOGY
(2023)
Meeting Abstract
Respiratory System
Laura Smith, Nina Pattabhiraman, Georgina Appleyard, Ian Hutchesson, Katie Jackson, Shavanthi Wadanamby, Alexander Lister, Jamal Bhatti, Scott Hemphill, Krzysztof Poterlowicz, Dinesh Saralaya
EUROPEAN RESPIRATORY JOURNAL
(2021)
Meeting Abstract
Dermatology
A. Mardaryev, M. van Lessen, F. J. Acosta, T. Biro, R. Paus
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2021)
Meeting Abstract
Dermatology
G. Chen, Q. Xu, M. Fessing, A. Mardaryev, A. Sharov, G. Xu, V. A. Botchkarev
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2021)
Meeting Abstract
Dermatology
A. Mardaryev, M. van Lessen, F. Jimenez Acosta, R. Paus, T. Biro
EXPERIMENTAL DERMATOLOGY
(2021)
Proceedings Paper
Agriculture, Multidisciplinary
A. N. Mardaryev, N. Mardaryeva, G. A. Larionov, V. S. Gordova, M. G. Terentyeva
INTERNATIONAL AGROSCIENCE CONFERENCE (AGROSCIENCE-2019)
(2020)
Meeting Abstract
Dermatology
G. Chen, Q. Xu, M. Fessing, A. Mardaryev, A. Sharov, G. Xu, V. A. Botchkarev
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2020)