Article
Neurosciences
Takahiro Fujimoto, Kirsten Stam, Takeshi Yaoi, Kenta Nakano, Tetsuya Arai, Tadashi Okamura, Kyoko Itoh
Summary: This study aimed to investigate the expression profile of Dp71 in the cerebellum and found its presence in glial cells, Bergmann glial cells, and astrocytes, while Dp427 was exclusively expressed in inhibitory postsynapses within cerebellar Purkinje cells. Additionally, the study revealed biochemical associations of Dp71 with AQP4 and Kir4.1 in both the cerebellum and cerebrum, and partial co-localization of Dp71 with AQP4 and Kir4.1 in glial cells. The results suggest that different cell types in the cerebellum express different dystrophin molecular complexes, which may play a role in pathological and physiological processes through the regulation of water/ion channels and inhibitory postsynapses.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Neurosciences
Vasiliki Tellios, Matthew J. E. Maksoud, Wei-Yang Lu
Summary: This study is the first to characterize BG morphology and GLAST expression during development in nNOS(-/-) mice using immunohistochemistry and western blotting. The results showed that BG in nNOS(-/-) mice exhibited abnormal morphology and decreased GLAST expression compared with wildtype (WT) mice across postnatal development. It was also found that nNOS/NO signaling regulates BG development through a PKG-mediated mechanism.
Article
Neurosciences
Angelica Salinas-Birt, Xiangyu Zhu, Eunice Y. Lim, Aryana J. Cruz J. Santory, Liang Ye, Martin Paukert
Summary: Behavioral state plays an important role in astroglia Ca2+ signaling, with locomotion-induced vigilance triggering norepinephrine-dependent Ca2+ elevations. The speed of locomotion has little effect on Ca2+ signals, but prolonged locomotion events result in steady-state Ca2+ elevation. Coordinated activity among noradrenergic terminals determines Bergmann glia Ca2+ activity.
Article
Biochemistry & Molecular Biology
Karthik Subramanian Krishnan, Brian Billups
Summary: D-serine is a crucial signaling molecule that works in tandem with glutamate to activate NMDA receptors. Astrocytes are hypothesized to control extracellular levels of D-serine by removing it from the synaptic space, suggesting that astrocytes play a significant role in regulating synaptic plasticity and memory.
Article
Biochemistry & Molecular Biology
America Vera-Montecinos, Jordi Galiano-Landeira, Monica Roldan, Francisco Vidal-Domenech, Enrique Claro, Belen Ramos
Summary: METTL7A is mainly located in the endoplasmic reticulum and lipid droplets, with limited cellular expression in the brain. Reduced protein levels of METTL7A have been found in schizophrenia. Our study shows that METTL7A is highly expressed in Bergmann glia and has contacts with Purkinje neurons. The localization of METTL7A may play a role in maintaining cerebellar homeostasis and modulating cerebellar circuits.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Neurosciences
Bumwhee Lee, Laura Beuhler, Hye Young Lee
Summary: The study revealed a reduction in the number of primary cilia and Sonic hedgehog (Shh) signaling in cerebellar Bergmann glia of Fmr1 KO mice, leading to reduced Granule Neuron Precursor (GNP) proliferation and thickness of the External Germinal Layer (EGL). This suggests that deficits in primary cilia of Bergmann glia may contribute to cerebellar developmental phenotypes in Fragile X Syndrome (FXS).
Article
Neurosciences
Ella Borgenheimer, Katherine Hamel, Carrie Sheeler, Francisco Labrada Moncada, Kaelin Sbrocco, Ying Zhang, Marija Cvetanovic
Summary: In the early stages of SCA1 in mice, there were no changes in the proportions of neurons and glial cells in the cerebellum, but Bergmann glia, velate astrocytes, and oligodendrocytes showed profound non-cell autonomous and potentially neuroprotective reactive gene and pathway alterations in response to Purkinje cell dysfunction.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Immunology
Chandrakanth Reddy Edamakanti, Vishwa Mohan, Puneet Opal
Summary: Using human SCA autopsy samples, researchers discovered inflammatory JNK-dependent c-Jun phosphorylation in Bergmann glia, and inhibiting the JNK pathway reduced Bergmann glia inflammation and improved the SCA1 phenotype both behaviorally and pathologically, suggesting a causal role for Bergmann glia inflammation in SCA1 and a potential therapeutic strategy.
JOURNAL OF NEUROINFLAMMATION
(2023)
Article
Neurosciences
Shelanah Salih, Zubair Ahmed Nizamudeen, Nigel De Melo, Lisa Chakrabarti, Virginie Sottile
Summary: Recent observations suggest that Bergmann glia in the cerebellum may play a role in tissue repair due to their expression of neural stem cell markers, although the physiological relevance of this overlap remains unclear in the absence of established in vivo evidence of tissue regeneration in the adult cerebellum.
EXPERIMENTAL NEUROLOGY
(2022)
Article
Neurosciences
Carmen Nanclares, Jose Antonio Noriega-Prieto, Francisco E. Labrada-Moncada, Marija Cvetanovic, Alfonso Araque, Paulo Kofuji
Summary: Spinocerebellar ataxia type 1 (SCA1) is a neurodegenerative disease characterized by progressive cerebellar ataxia. This study found that the intrinsic electrical properties of Purkinje cells (PCs) in SCA1 mice were altered, and these alterations were associated with the hyperexcitability of Bergmann glia (BG). Preventing BG hyperexcitability in SCA1 mice restored the normal function of PCs.
NEUROBIOLOGY OF DISEASE
(2023)
Article
Neurosciences
Vanessa L. Hull, Yan Wang, Travis Burns, Sarah Sternbach, Shuaishuai Gong, Jennifer McDonough, Fuzheng Guo, Laura N. Borodinsky, David Pleasure
Summary: Canavan disease is a pediatric leukodystrophy caused by mutations in the ASPA gene, resulting in a deficiency of the enzyme aspartoacylase. This leads to increased levels of N-acetyl-L-aspartate (NAA) in the brain and various neurological symptoms. In a mouse model of Canavan disease, researchers found that Bergmann glia (BG) exhibited significant morphological alterations and dysfunction, which preceded cerebellar degeneration. However, treatment with an antisense oligonucleotide targeting Nat8l, which reduces NAA production, was able to repair the BG and improve motor function. This suggests that restoring BG integrity may be a potential therapeutic strategy for Canavan disease.
Article
Biochemistry & Molecular Biology
Xia Li, Romain Helleringer, Lora L. Martucci, Glenn Dallerac, Jose-Manuel Cancela, Micaela Galante
Summary: Cerebral ischemia, which is caused by a reduction or interruption in blood supply to the brain, leads to deprivation of oxygen and glucose. The consequences of cerebral ischemia include loss of metabolic ATP, accumulation of K+ and glutamate in the extracellular space, electrolyte imbalance, and brain edema formation. This study focuses on the neuroprotective role of hypothermia in a mouse cerebellar slice model of ischemia, and found that lowering the temperature delays the increase of K+ and tissue swelling. In addition, hypothermia hinders the morphological changes and membrane depolarizations of Bergmann glial cells, suggesting that it reduces harmful homeostatic changes in cerebellar ischemia.
Review
Cell Biology
Daniel Z. Radecki, Jayshree Samanta
Summary: Oligodendrogenesis in the adult mammalian brain varies in different regions, with the subventricular zone and the subgranular zone being the primary regions of adult neurogenesis.
Article
Multidisciplinary Sciences
Lech Kaczmarczyk, Nicole Reichenbach, Nelli Blank, Maria Jonson, Lars Dittrich, Gabor C. Petzold, Walker S. Jackson
Summary: Genetic variation is a key factor in determining phenotypic diversity, which can be minimized through inbreeding. While most disease models and transgenic tools are in C57Bl/6, caution must be exercised when generalizing results obtained with inbred strains, especially in studies involving complex phenotypes and disease models.
SCIENTIFIC REPORTS
(2021)
Article
Neurosciences
Anton N. Shuvaev, Olga S. Belozor, Oleg Mozhei, Dariya A. Yakovleva, Ilya V. Potapenko, Andrey N. Shuvaev, Marina V. Smolnikova, Vladimir V. Salmin, Alla B. Salmina, Hirokazu Hirai, Anja G. Teschemacher, Sergey Kasparov
Summary: In cerebellar neurodegenerative diseases such as SCA1, reactive BG can negatively impact neuronal function and survival through compromised glutamate uptake. Excessive glutamate signaling appears to be a common feature in SCA1 pathology, contributing to cerebellar neurodegeneration.
NEUROBIOLOGY OF DISEASE
(2021)
Article
Neurosciences
Xiang-Dong Sun, Lei Li, Fang Liu, Zhi-Hui Huang, Jonathan C. Bean, Hui-Feng Jiao, Arnab Barik, Seon-Myung Kim, Haitao Wu, Chengyong Shen, Yun Tian, Thiri W. Lin, Ryan Bates, Anupama Sathyamurthy, Yong-Jun Chen, Dong-Min Yin, Lei Xiong, Hui-Ping Lin, Jin-Xia Hu, Bao-Ming Li, Tian-Ming Gao, Wen-Cheng Xiong, Lin Mei
NATURE NEUROSCIENCE
(2016)
Article
Medicine, Research & Experimental
Chengyong Shen, Yisheng Lu, Bin Zhang, Dwight Figueiredo, Jonathan Bean, Jiung Jung, Haitao Wu, Arnab Barik, Dong-Min Yin, Wen-Cheng Xiong, Lin Mei
JOURNAL OF CLINICAL INVESTIGATION
(2013)
Article
Neurosciences
Jonathan C. Bean, Thiri W. Lin, Anupama Sathyamurthy, Fang Liu, Dong-Min Yin, Wen-Cheng Xiong, Lin Mei
JOURNAL OF NEUROSCIENCE
(2014)
Article
Neurosciences
Yisheng Lu, Xiang-Dong Sun, Feng-Qing Hou, Lin-Lin Bi, Dong-Min Yin, Fang Liu, Yong-Jun Chen, Jonathan C. Bean, Hui-Feng Jiao, Xihui Liu, Bao-Ming Li, Wen-Cheng Xiong, Tian-Ming Gao, Lin Mei
Article
Multidisciplinary Sciences
Sashaina E. Fanibunda, Sukrita Deb, Babukrishna Maniyadath, Praachi Tiwari, Utkarsha Ghai, Samir Gupta, Dwight Figueiredo, Noelia Weisstaub, Jay A. Gingrich, Ashok D. B. Vaidya, Ullas Kolthur-Seetharam, Vidita A. Vaidya
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Immunology
Radika Soysa, Jonathan C. Bean, Xia Wu, Sarah Lampert, Sebastian Yuen, Ian N. Crispe
Summary: The study revealed that early-derived subsets of cardiac macrophages and Kupffer cells exhibit resilience in the face of acute stress by temporarily losing adaptation to local tissue-specific niches while reasserting their generic myeloid identity.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Longlong Tu, Nan Zhang, Kristine M. Conde, Jonathan C. Bean, Chunmei Wang, Yong Xu
Summary: This paper presents a reliable workflow for immunohistochemical staining of mouse brains, with detailed steps and how to modify procedures to meet individual researchers' needs. The protocol's reliability and efficiency were demonstrated through staining experiments.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Cell Biology
Emily J. Koller, Melissa Comstock, Jonathan C. Bean, Gabriel Escobedo, Kyung-Won Park, Joanna L. Jankowsky
Summary: This paper describes two strategies for controlling disease-associated amyloid precursor protein (APP) expression in modeling Alzheimer's amyloid pathology using Cre and CreER mouse lines. The first strategy combines a Cre driver with a tetracycline-transactivator (tTA)-dependent APP responder to achieve spatial and temporal control over APP expression. The second strategy involves using an intervening lox-stop-lox cassette to directly control APP expression through Cre recombinase, which can be paired with a CreER driver for spatial and temporal control.
DISEASE MODELS & MECHANISMS
(2022)
Article
Biochemistry & Molecular Biology
Meng Yu, Jonathan C. Bean, Hailan Liu, Yang He, Yongjie Yang, Xing Cai, Kaifan Yu, Zhou Pei, Hesong Liu, Longlong Tu, Kristine M. Conde, Mengjie Wang, Yongxiang Li, Na Yin, Nan Zhang, Junying Han, Nikolas A. Scarcelli, Pingwen Xu, Yanlin He, Yong Xu, Chunmei Wang
Summary: This study revealed the sexually dimorphic role of SK3 in POMC neurons in both energy and glucose homeostasis, independent of body weight control. The deficiency of SK3 specifically affected glucose balance in female mice but not in male mice. The results provided evidence for the sex difference in the function of SK3 in POMC neurons.
CELL AND BIOSCIENCE
(2022)
Article
Endocrinology & Metabolism
Zhou Pei, Yang He, Jonathan C. Bean, Yongjie Yang, Hailan Liu, Meng Yu, Kaifan Yu, Ilirjana Hyseni, Xing Cai, Hesong Liu, Na Qu, Longlong Tu, Kristine M. Conde, Mengjie Wang, Yongxiang Li, Na Yin, Nan Zhang, Junying Han, Camille HS. Potts, Nikolas A. Scarcelli, Zili Yan, Pingwen Xu, Qi Wu, Yanlin He, Yong Xu, Chunmei Wang
Summary: Pro-opiomelanocortin (POMC) neurons play a crucial role in regulating body weight and glucose balance. In this study, the researchers investigated the role of Gabra5, a subunit of the GABA(A) receptor, in POMC neurons. They found that knocking down Gabra5 in POMC neurons increased firing frequency and resting membrane potential in male mice, leading to improved glucose tolerance. However, this effect was not observed in female mice. These findings suggest a sexually dimorphic role of Gabra5 in POMC neuron activity and glucose balance, independent of body weight control.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yang He, Bas Brouwers, Hesong Liu, Hailan Liu, Katherine Lawler, Edson Mendes de Oliveira, Dong-Kee Lee, Yongjie Yang, Aaron R. Cox, Julia M. Keogh, Elana Henning, Rebecca Bounds, Aliki Perdikari, Vikram Ayinampudi, Chunmei Wang, Meng Yu, Longlong Tu, Nan Zhang, Na Yin, Junying Han, Nikolas A. Scarcelli, Zili Yan, Kristine M. Conde, Camille Potts, Jonathan C. Bean, Mengjie Wang, Sean M. Hartig, Lan Liao, Jianming Xu, Ines Barroso, Jacek Mokrosinski, Yong Xu, I. Sadaf Farooqi
Summary: Serotonin 2C receptor (5-HT2CR) plays a role in the regulation of human appetite, weight and behavior. Loss-of-function variants in the HTR2C gene are associated with obesity and maladaptive behavior. Activation of appetite-suppressing neurons is impaired in mice carrying HTR2C variants.
Article
Medicine, Research & Experimental
Longlong Tu, Jonathan C. Bean, Yang He, Hailan Liu, Meng Yu, Hesong Liu, Nan Zhang, Na Yin, Junying Han, Nikolas A. Scarcelli, Kristine M. Conde, Mengjie Wang, Yongxiang Li, Bing Feng, Peiyu Gao, Zhao-Lin Cai, Makoto Fukuda, Mingshan Xue, Qingchun Tong, Yongjie Yang, Lan Liao, Jianming Xu, Chunmei Wang, Yanlin He, Yong Xu
Summary: Glucose-inhibited neurons paradoxically increase firing activity in low-glucose conditions and decrease in high-glucose conditions. This study reveals that the Ano4 channel mediates the electric responses of these neurons to glucose fluctuations.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Endocrinology & Metabolism
Hailan Liu, Yang He, Juli Bai, Chuanhai Zhang, Feng Zhang, Yongjie Yang, Hairong Luo, Meng Yu, Hesong Liu, Longlong Tu, Nan Zhang, Na Yin, Junying Han, Zili Yan, Nikolas Anthony Scarcelli, Kristine Marie Conde, Mengjie Wang, Jonathan Carter Bean, Camille Hollan Sidell Potts, Chunmei Wang, Fang Hu, Feng Liu, Yong Xu
Summary: A study found that the adaptor protein Grb10 can enhance leptin signaling and reduce appetite through regulating the activity of AgRP and POMC neurons. Overexpression of Grb10 in AgRP neurons can decrease body weight, while in POMC neurons it can attenuate diet-induced obesity. The mechanism may involve enhancing leptin's effects on neurons through regulating KATP and TRP channels.
