Article
Gastroenterology & Hepatology
Diana Raquel Rodriguez-Rodriguez, Sonia Amelia Lozano-Sepulveda, Cecilia Delgado-Montemayor, Noemi Waksman, Paula Cordero-Perez, Ana Maria Rivas-Estilla
Summary: The study investigated the mechanism of hepatic fibrosis modulation by a methanolic extract of Turnera diffusa on HSC cells. Results showed that the extract reduced profibrotic and mitochondrial markers while inducing apoptosis through SNAI1 expression.
ANNALS OF HEPATOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ingrid Wilhelmsen, Mikel Amirola Martinez, Justyna Stokowiec, Chencheng Wang, Aleksandra Aizenshtadt, Stefan Krauss
Summary: This study investigates the phenotypic and functional development of pluripotent stem cell-derived hepatic stellate cells (scHSCs) and liver sinusoidal endothelial cells (scLSECs) during extended in vitro culture. It is crucial to consider the choice of pluripotent stem cell line and experimental timeframe in designing in vitro platforms involving scHSCs and scLSECs. Notch inhibition and co-culturing scHSCs and scLSECs have shown potential in enhancing the stability and maturity of these cells.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemical Research Methods
Julia Vallverdu, Raquel A. Martinez Garcia de la Torre, Inge Mannaerts, Stefaan Verhulst, Ayla Smout, Mar Coll, Silvia Arino, Teresa Rubio-Tomas, Beatriz Aguilar-Bravo, Celia Martinez-Sanchez, Delia Blaya, Catherine M. Verfaillie, Leo A. van Grunsven, Pau Sancho-Bru
Summary: Human iPSCs are differentiated into HSCs with growth factors for in vitro modeling. The protocol yields iPSC-HSCs with phenotypic and functional characteristics of primary HSCs, suitable for high-throughput in vitro studies. Coculturing iPSC-HSCs with hepatocytes allows for the formation of 3D hepatic spheroids, enabling modeling and drug screening studies.
Article
Cell Biology
Alexandre Balaphas, Jeremy Meyer, Cecile Gameiro, Aurelien Frobert, Marie-Noelle Giraud, Bernhard Egger, Leo H. Buhler, Carmen Gonelle-Gispert
Summary: The aim of this study was to optimize the isolation of hepatic stellate cells (HSCs) from mouse livers and obtain highly pure HSC populations. Through a two-step collagenase digestion and Nycodenz gradient separation followed by CD11b-negative sorting, we successfully isolated HSCs with high yield, viability, and purity. Flow cytometry analysis showed that CD38 may be a reliable marker for HSCs.
Article
Multidisciplinary Sciences
Steffen K. Meurer, David A. Brenner, Ralf Weiskirchen
Summary: Misidentification, cross-contamination, and genetic drift of continuous animal cell lines are common problems in biomedical research, which can lead to erroneous results and inconsistent or invalidated studies. This study established a multi-loci short tandem repeat signature for the immortalized hepatic stellate cell line Col-GFP HSC, allowing for unique cell line authentication.
Article
Cell Biology
Indrajit Nanda, Claus Steinlein, Thomas Haaf, Eva M. Buhl, Domink G. Grimm, Scott L. Friedman, Steffen K. Meurer, Sarah K. Schroeder, Ralf Weiskirchen
Summary: Immortalized hepatic stellate cells (HSCs) are valuable tools for liver biology research, but their use has some drawbacks, such as genetic drift and cell misidentification. This study characterizes the genetic features of the rat HSC line HSC-T6 and proposes cell line authentication.
Article
Biochemistry & Molecular Biology
Koichi Fujisawa, Yuto Nishimura, Akino Sakuragi, Jolien Duponselle, Toshihiko Matsumoto, Naoki Yamamoto, Tomoaki Murata, Isao Sakaida, Taro Takami
Summary: Research has been conducted to develop new medical treatments by simulating environments existing in space, such as zero-gravity. In this study, cell proliferation and gene expression of activated primary human hepatic stellate cells (HHSteCs) under simulated microgravity (SMG) were evaluated. The findings showed slower cell proliferation under SMG conditions, as well as changes in gene expression and oxidative stress. Upstream regulators were activated and cell-permeable inhibitors were inhibited, and cytoskeletal changes were observed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Toxicology
Jinmao Liao, Zheng Zhang, Qi Yuan, Qiong Liu, Jia Kuang, Yuan Fang, Xiaoxuan Hu
Summary: The study identified a lncRNA Gpr137b-ps/miR-200a-3p/CXCL14 axis that regulates HSC activation and may have an impact on the pathogenesis of liver fibrosis. The silencing of Gpr137b-ps or CXCL14 alone improved fibrotic changes induced by CCl4 in mice liver tissue and HSCs in vivo and in vitro. MiR-200a-3p directly targeted Gpr137b-ps and CXCL14, indicating their role in mediating hepatic fibrosis.
TOXICOLOGY LETTERS
(2021)
Article
Immunology
Fangming Yang, Heng Li, Yanmin Li, Yaokun Hao, Chenxiao Wang, Pan Jia, Xinju Chen, Suping Ma, Zhun Xiao
Summary: Hepatic fibrosis is a dynamic pathological process characterized by the accumulation of extra cellular matrix in chronic liver diseases, with activation of hepatic stellate cells (HSCs) playing a central role. Communication between hepatocytes and surrounding cells influences the activation of HSCs and the progression of fibrosis, highlighting the complexity and plasticity of HSC activation.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Maximilian Schinagl, Tamara Tomin, Juergen Gindlhuber, Sophie Honeder, Raphael Pfleger, Matthias Schittmayer, Michael Trauner, Ruth Birner-Gruenberger
Summary: This study investigated the activation of hepatic stellate cells (HSC) through an increase in growth medium serum concentration, revealing cellular processes involved in HSC transformation. Activated HSC showed increased production of ribosomal proteins and proteins related to migration and cell cycle control, along with a decrease in cholesterol and fatty acid biosynthesis proteins. The findings provide insights into HSC activation characteristics and present a accessible model for studying HSC activation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agriculture, Multidisciplinary
Yu Chen, Lizhen Lin, Chunhong Yang, Tao Li, Yuan Li, Jian Wang, Yanling Wu, Yuqing Zhao, Guangyue Su
Summary: The study found that ginsenoside 25-hydroxy protopanaxadiol (AD-2) from ginseng has anti-hepatic fibrosis effects. It inhibits cell activation and improves liver injury and fibrosis. AD-2 also regulates the expression of related genes and the vitamin D/receptor axis.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Cell Biology
Brandon T. Wesley, Alexander D. B. Ross, Daniele Muraro, Zhichao Miao, Sarah Saxton, Rute A. Tomaz, Carola M. Morell, Katherine Ridley, Ekaterini D. Zacharis, Sandra Petrus-Reurer, Judith Kraiczy, Krishnaa T. Mahbubani, Stephanie Brown, Jose Garcia-Bernardo, Clara Alsinet, Daniel Gaffney, Dave Horsfall, Olivia C. Tysoe, Rachel A. Botting, Emily Stephenson, Dorin-Mirel Popescu, Sonya MacParland, Gary Bader, Ian D. McGilvray, Daniel Ortmann, Fotios Sampaziotis, Kourosh Saeb-Parsy, Muzlifah Haniffa, Kelly R. Stevens, Matthias Zilbauer, Sarah A. Teichmann, Ludovic Vallier
Summary: In this study, the developmental trajectories of human fetal liver cell types were described at single-cell resolution, and bipotential hepatoblast organoids were generated. This provides a platform for investigating human liver development and producing cell types for clinical applications.
NATURE CELL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Hany Ezzat Khalil, Hairul-Islam Mohamed Ibrahim, Kareem Ahmed El-Fass, Sabah H. Akrawi, Mohamed A. Morsy
Summary: Orientin has anti-inflammatory effects and can reduce the risk of liver inflammation and fibrosis. It protects against liver injury by inhibiting fibrotic markers and reducing pro-inflammatory mediators.
APPLIED SCIENCES-BASEL
(2022)
Review
Neurosciences
Dakota R. Kamm, Kyle S. McCommis
Summary: Hepatic stellate cells (HSCs) play critical roles in the normal liver and in response to injury. They are activated during liver injury and produce extracellular matrix in liver fibrosis. In the absence of injury, HSCs are in a quiescent state and store retinoids or vitamin A-containing metabolites. They also enhance the inflammatory response and express growth factors crucial for liver development and regeneration. Recent studies have identified diverse phenotypic alterations and unique subpopulations of HSCs.
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Article
Cell Biology
Cleverson Moraes de Oliveira, Leo Anderson Meira Martins, Arieli Cruz de Sousa, Ketlen da Silveira Moraes, Bruna Pasqualotto Costa, Moema Queiroz Vieira, Barbara Paranhos Coelho, Radovan Borojevic, Jarbas Rodrigues de Oliveira, Fatima Costa Rodrigues Guma
Summary: Resveratrol did not decrease the activation state of GRX and instead triggered a pro-activation effect at the 50 μM concentration. Despite the increase of TNF-α in culture media, the results on IL-6 and IL-10 secretion were in accordance with the anti-inflammatory role of Resveratrol in the model.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2021)
Article
Gastroenterology & Hepatology
Martin L. Decaris, Kelvin W. Li, Claire L. Emson, Michelle Gatmaitan, Shanshan Liu, Yenny Wang, Edna Nyangau, Marc Colangelo, Thomas E. Angel, Carine Beysen, Jeffrey Cui, Carolyn Hernandez, Len Lazaro, David A. Brenner, Scott M. Turner, Marc K. Hellerstein, Rohit Loomba
Meeting Abstract
Gastroenterology & Hepatology
Hyeok Choon Kwon, Jun Xu, Hsiao-Yen Ma, Linshan Shang, Tatiana Kisseleva, David A. Brenner
Meeting Abstract
Gastroenterology & Hepatology
Yukinori Koyama, Ronglin Hu, Jun Xu, Xiao Liu, Hsiao-Yen Ma, Shuang Liang, David A. Brenner, Tatiana Kisseleva
Meeting Abstract
Gastroenterology & Hepatology
Jun Xu, Shuang Liang, Hsiao-Yen Ma, Mengxi Sun, Yukinori Koyama, Tatiana Kisseleva, David A. Brenner
Article
Gastroenterology & Hepatology
Charlie C. Park, Phirum Nguyen, Carolyn Hernandez, Ricki Bettencourt, Kimberly Ramirez, Lynda Fortney, Jonathan Hooker, Ethan Sy, Michael T. Savides, Mosab H. Alquiraish, Mark A. Valasek, Emily Rizo, Lisa Richards, David Brenner, Claude B. Sirlin, Rohit Loomba
Article
Medicine, Research & Experimental
Min Cong, Weihua Zhao, Tianhui Liu, Ping Wang, Xu Fan, Qingling Zhai, Xiaoli Bao, Dong Zhang, Hong You, Tatiana Kisseleva, David A. Brenner, Jidong Jia, Hui Zhuang
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2017)
Article
Medicine, Research & Experimental
Yukinori Koyama, Ping Wang, Shuang Liang, Keiko Iwaisako, Xiao Liu, Jun Xu, Mingjun Zhang, Mengxi Sun, Min Cong, Daniel Karin, Kojiro Taura, Chris Benner, Sven Heinz, Tapan Bera, David A. Brenner, Tatiana Kisseleva
JOURNAL OF CLINICAL INVESTIGATION
(2017)
Article
Medicine, Research & Experimental
Cyrielle Caussy, Meera Soni, Jeffrey Cui, Ricki Bettencourt, Nicholas Schork, Chi-Hua Chen, Mahdi Al Ikhwan, Shirin Bassirian, Sandra Cepin, Monica P. Gonzalez, Michel Mendler, Yuko Kono, Irine Vodkin, Kristin Mekeel, Jeffrey Haldorson, Alan Hemming, Barbara Andrews, Joanie Salotti, Lisa Richards, David A. Brenner, Claude B. Sirlin, Rohit Loomba
JOURNAL OF CLINICAL INVESTIGATION
(2017)
Article
Medicine, Research & Experimental
Elisabeth H. Vollmann, Lizhi Cao, Aldo Amatucci, Taylor Reynolds, Stefan Hamann, Isin Dalkilic-Liddle, Thomas O. Cameron, Markus Hossbach, Kevin J. Kauffman, Faryal F. Mir, Daniel G. Anderson, Tatiana Novobrantseva, Victor Koteliansky, Tatiana Kisseleva, David Brenner, Jeremy Duffield, Linda C. Burkly
MOLECULAR THERAPY-NUCLEIC ACIDS
(2017)
Article
Gastroenterology & Hepatology
Jun Xu, Hsiao-Yen Ma, Shuang Liang, Mengxi Sun, Gabriel Karin, Yukinori Koyama, Ronglin Hu, Oswald Quehenberger, Nicholas O. Davidson, Edward A. Dennis, Tatiana Kisseleva, David A. Brenner
HEPATOLOGY COMMUNICATIONS
(2017)
Letter
Gastroenterology & Hepatology
Megan E. Reinders, Gabriel Wardi, Ricki Bettencourt, Daniel Bouland, Jessica Bazick, Michel Mendler, Irine Vodkin, Denise Kalmaz, Thomas Savides, David Brenner, Rebecca E. Sell, Rohit Loomba
CLINICAL GASTROENTEROLOGY AND HEPATOLOGY
(2017)
Article
Cell Biology
Rohit Loomba, Victor Seguritan, Weizhong Li, Tao Long, Niels Klitgord, Archana Bhatt, Parambir Singh Dulai, Cyrielle Caussy, Richele Bettencourt, Sarah K. Highlander, Marcus B. Jones, Claude B. Sirlin, Bernd Schnabl, Lauren Brinkac, Nicholas Schork, Chi-Hua Chen, David A. Brenner, William Biggs, Shibu Yooseph, J. Craig Venter, Karen E. Nelson
Letter
Gastroenterology & Hepatology
Niraj S. Patel, Jonathan Hooker, Monica Gonzalez, Archana Bhatt, Phirum Nguyen, Kimberly Ramirez, Lisa Richards, Emily Rizo, Carolyn Hernandez, Tatiana Kisseleva, Bernd Schnabl, David Brenner, Claude B. Sirlin, Rohit Loomba
CLINICAL GASTROENTEROLOGY AND HEPATOLOGY
(2017)
Article
Cell Biology
Mark A. Chapman, Kavitha Mukund, Shankar Subramaniam, David Brenner, Richard L. Lieber
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2017)