Review
Nutrition & Dietetics
Izabela Nawrot-Hadzik, Adam Matkowski, Jakub Hadzik, Barbara Dobrowolska-Czopor, Cyprian Olchowy, Marzena Dominiak, Pawel Kubasiewicz-Ross
Summary: Flavan-3-ols and proanthocyanidins are polyphenolic compounds with antioxidant, anti-inflammatory, and antimicrobial properties that play a crucial role in preventing and treating periodontitis.
Article
Biochemistry & Molecular Biology
Javier I. Ottaviani, Reedmond Y. Fong, Gina Borges, Jennifer Kimball, Jodi L. Ensunsa, Valentina Medici, L. Kirsty Pourshahidi, Emma Kane, Karen Ward, Rebecca Durkan, Sara Dobani, Roger Lawther, Gloria O'Connor, Chris I. R. Gill, Hagen Schroeter, Alan Crozier
Summary: This study aimed to elucidate the interactions between flavan-3-ols and methylxanthines by evaluating the specific contributions of theobromine and caffeine on flavan-3-ol bioavailability. The results demonstrated a differential effect of caffeine and theobromine on modulating flavan-3-ol bioavailability when co-ingested with bioactives. These findings are important for optimizing current dietary recommendations for flavan-3-ol intake.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Food Science & Technology
Junfang Wang, Rui Zhang, Jinzhu Jiang, Wei Duan, Peige Fan, Shaohua Li, Lijun Wang
Summary: An orthogonal L(16)4(3) x 2(2) test design was used to optimize the extraction of flavan-3-ols in grape seeds. The optimized HPLC method separated and identified fourteen flavan-3-ols, showing that the new wine grape - Beihong, had higher flavan-3-ols content and polymerization compared to classic wine grapes like Cabernet Sauvignon, Merlot, Semillon, and Riesling.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Agriculture, Multidisciplinary
Haibo Pan, Yi Wang, Xinlei Xu, Ziqi Qian, Huan Cheng, Xingqian Ye, Shiguo Chen
Summary: Biorefineries of polyphenols from plant leaves aim to maximize their commercial value, but the presence of condensed tannins (CTs) hinders their bioavailability. A one-step method using acid catalysis was developed to extract polyphenols and depolymerize CTs, leading to improved bioavailability. Purification with specific polymeric resins, such as Amberlite XAD-2, showed high efficiency in enhancing the bioavailability and activity of polyphenols from plant leaves.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Review
Nutrition & Dietetics
Izabela Nawrot-Hadzik, Adam Matkowski, Pawel Kubasiewicz-Ross, Jakub Hadzik
Summary: Phytochemicals have bidirectional activity in inhibiting bacterial proliferation and proinflammatory signaling, providing substantial alleviation of periodontal disease cause and symptoms.
Article
Food Science & Technology
Tony Y. Y. Momma, Gunter G. C. Kuhnle, Reedmond Y. Y. Fong, Jodi L. L. Ensunsa, Alan Crozier, Hagen Schroeter, Javier I. I. Ottaviani
Summary: This study discovers that a type of human protein called PON is involved in the metabolism of flavan-3-ols. It is found that PON can rapidly convert one metabolite, & gamma;VL, into its corresponding metabolite, & gamma;VA, and can also react with other flavan-3-ol metabolites. The study also reveals that common PON gene polymorphisms have a minor contribution to the level differences of & gamma;VL metabolites, but do not affect their use as nutritional biomarkers.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Article
Food Science & Technology
Paulo Wender P. Gomes, Emilli Roberta S. Gomes, Alice R. V. Carvalho, Helena Mannochio-Russo, Tiago F. Leao, Jose Diogo E. Reis, Maria Rosilda V. de Sarges, Horrana A. Mardegan, Sonia das G. S. R. Pamplona, Consuelo Yumiko Y. Esilva, Milton N. da Silva
Summary: This study explores the presence of high content of catechin/ (epi)catechin derivatives in Stryphnodendron pulcherrimum, a plant found in the Brazilian Amazon. The discovery of condensed tannins and their potential benefits to gut microbiome metabolism is highlighted. The study also identifies a new tetrameric condensed tannin and suggests its potential role in preventing diseases and maintaining gut well-function.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Article
Nutrition & Dietetics
Yuko Ishii, Orie Muta, Tomohiro Teshima, Nayuta Hirasima, Minayu Odaka, Taiki Fushimi, Yasuyuki Fujii, Naomi Osakabe
Summary: The study demonstrated that flavan 3-ol (FLs) may induce browning of adipose tissue in mice by activating the sympathetic nervous system (SNS), leading to increased expression of uncoupling protein (Ucp)-1 and thermogenic transcriptome factors. This effect was confirmed by a marked increase in urinary catecholamine (CA) levels, suggesting enhanced SNS activity.
Article
Agriculture, Multidisciplinary
Chengcheng Sun, Guangjian Li, Hongbiao Li, Yunbin Lyu, Shiqin Yu, Jingwen Zhou
Summary: This study successfully achieved de novo production of two basic flavan-3-ols by engineering Saccharomyces cerevisiae, demonstrating the potential of engineering strategies in increasing yield.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Agronomy
Changmei Liang, Jianyong Guo, Mingxiang Chen, Xuehui Zhang, Guorong Zhang, Pengfei Zhang, Jinjun Liang, Pengfei Wen
Summary: The study found that VvMYBPA2 regulates the biosynthesis of flavan-3-ol monomers in grapes. It interacts with VvWDR1 and VvMYC2 to form a complex, jointly regulating the expression of genes VvANR and VvLAR1, affecting the enzyme activities of ANR and LAR, and ultimately regulating the contents of flavan-3-ols.
Article
Agriculture, Multidisciplinary
Gabriel R. Martins, Mariana M. G. Mattos, Fabiane Marques Nascimento, Felipe L. Brum, Ronaldo Mohana-Borges, Natalia Guimaraes Figueiredo, Domingos F. M. Neto, Gilberto Barbosa Domont, Fabio Cesar Sousa Nogueira, Francisco de Assis de Paiva Campos, Ayla Sant'Ana da Silva
Summary: Changes in the phenolic profile and antioxidant properties were investigated in developing acai seeds. Earlier stages displayed higher antioxidant activity and polyphenols content, with procyanidins identified as the major components. Extracted procyanidins decreased in length along seed development, with (-)-epicatechin being the predominant unit and (+)-catechin increasing in presence. This variation was correlated to the abundance of key enzymes for procyanidin biosynthesis.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Organic
Shu-Xi Jing, Daneel Ferreira, Pankaj Pandey, Larry L. Klein, Amar Gopal Chittiboyina, James B. Mcalpine, David C. Lankin, Yvette Alania, Mariana Reis-Havlat, Ana K. Bedran-Russo, Shao-Nong Chen, Guido F. Pauli
Summary: This study discovered novel trimeric and tetrameric proanthocyanidins (PACs) with unprecedented benzoquinone motifs. Comprehensive spectroscopic analysis, including NMR and ECD, provided vital stereochemical information. This is the first indication that PACs may achieve structural diversity through oxidative oligomerization and post-oligomerizational modifications.
JOURNAL OF ORGANIC CHEMISTRY
(2023)
Article
Agronomy
Tabasum Ara, Sabeeha Shafi, Mohammed Ghazwani, Javid Iqbal Mir, Abdul Haseeb Shah, Raies A. Qadri, Abdulrahim R. Hakami, Mohammad Khalid, Umme Hani, Shadma Wahab
Summary: Walnut leaves are rich in nutrients, minerals, and antioxidants, and have high antioxidant, anticancer, and antifungal activities.
Review
Food Science & Technology
Xuwei Liu, Carine Le Bourvellec, Sylvain Guyot, Catherine M. G. C. Renard
Summary: Flavanols, a subgroup of polyphenols with antioxidant properties, are naturally produced in plants and have significant effects on human health. However, their changes during physical processing, such as oxidation, polymerization, degradation, and epimerization, are often underestimated due to analytical challenges. Research on the effects of physical processing techniques on flavanol content and structural changes is important for improving nutritional value, but further studies are needed to better understand the role of flavanols in food processing.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2021)
Article
Nutrition & Dietetics
Kristi M. Crowe-White, Levi W. Evans, Gunter G. C. Kuhnle, Dragan Milenkovic, Kim Stote, Taylor Wallace, Deepa Handu, Katelyn E. Senkus
Summary: Guideline recommendation for the intake of plant bioactive such as flavan-3-ols is based on evidence of health improvement rather than nutritional deficiencies. Moderate evidence suggests that consuming 400-600 mg/d of flavan-3-ols can provide cardiometabolic protection and improve blood pressure, cholesterol concentrations, and blood sugar. This food-based guideline is important for informing multiple stakeholders and guiding future research.
ADVANCES IN NUTRITION
(2022)
Article
Agriculture, Multidisciplinary
Jose M. Villalgordo, Laura Trulli, Rocio Garcia-Villalba, Victor Garcia, Yusuf Althobaiti, Francisco A. Tomas-Barberan
Summary: Urolithins are human gut microbiota metabolites produced from ellagic acid. They are better absorbed than ellagic acid and have biological activities that may be responsible for the health effects observed after consuming foods containing ellagitannins and ellagic acid. This study successfully synthesized urolithin glucuronide conjugates and characterized their properties.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Review
Food Science & Technology
Ryan Sweet, Paul A. Kroon, Mark A. Webber
Summary: The risk of bacterial foodborne infection to human health is currently controlled by the addition of antimicrobial preservatives to food. However, the use of chemical preservatives poses health risks, making the development of improved preservatives a priority for the food industry. Plant-derived phytochemicals, particularly polyphenols, are a promising source of novel antimicrobial compounds. While there has been significant study of phytochemicals, there remains uncertainty regarding their relative efficacy, mechanisms of action, and potential for bacterial resistance.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Biochemical Research Methods
Amy E. Leaney, Jenna Heath, Emma Midforth, Paul Beck, Paul Brown, Deborah H. Mawson
Summary: This study investigated the content of Higenamine in food and supplements, and found a link between beetroot and this banned substance listed by WADA. The results demonstrated that Higenamine can arise within beetroot extracts through heating. The risk of inadvertent doping violation by consuming these products is low, but athletes should consider beetroot as a source of Higenamine.
DRUG TESTING AND ANALYSIS
(2023)
Article
Biophysics
Claudia Pigliacelli, Peter Belton, Peter Wilde, Francesca Baldelli Bombelli, Paul A. Kroon, Mark S. Winterbone, Sheng Qi
Summary: Formulating poorly soluble drugs with polymers as solid dispersions is a common method for improving drug dissolution. This study found that endogenous surface-active species in the gut, such as bile salts and lecithin, play a key role in enhancing the solubilization of lipids and poorly soluble drugs. The addition of a model bile salt, sodium taurocholate (NaTC), to spray dried solid dispersions containing piroxicam and Hydroxypropyl Methylcellulose (HPMC) further improved drug solubilization and resulted in the formation of NaTC-HPMC complexes and other mixed colloidal species. Cellular level absorption studies confirmed that the combination of solid dispersion delivery, bile salts, and lecithin significantly enhanced drug absorption.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Agriculture, Multidisciplinary
Ana M. Ares, Laura Toribio, Rocio Garcia-Villalba, Jose M. Villalgordo, Yusuf Althobaiti, Francisco A. Tomas-Barberan, Jose Bernal
Summary: Urolithins are metabolites produced in humans after consuming foods with ellagitannins and ellagic acid. This study reports a novel method for separating different isomers of urolithin glucuronides using supercritical fluid chromatography. The proposed method successfully analyzed these metabolites in urine samples from volunteers of different metabotypes.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Gabriela L. Salazar-Orbea, Rocio Garcia-Villalba, Maria J. Bernal, Alberto Hernandez-Jimenez, Jose A. Egea, Francisco A. Tomas-Barberan, Luis M. Sanchez-Siles
Summary: This research examined the impact of different storage conditions on the bioactive phenolic compounds, color, and sensory attributes of strawberry and apple purees produced with various industrial technologies. The study found that storage conditions had a stronger influence on phenolic compound levels in strawberry puree, particularly anthocyanins, while the initial processing techniques of apple puree had a greater impact than storage conditions. Proanthocyanidins were the most stable phenolic group during storage, while anthocyanins were the most affected. The stability of polyphenols varied between fruits during storage, and both processing and storage could have detrimental or beneficial effects.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Nutrition & Dietetics
Maja Bensa, Irena Vovk, Vesna Glavnik
Summary: The food supplement market is growing due to consumers' desire to supplement their nutrient intake. However, despite regulations in place, irregularities in the industry, particularly in internet sales, are still common. An evaluation of 20 resveratrol food supplement products sold in Slovenia showed that 95% had different contents than declared, with 55% containing higher contents. Additionally, there were errors and misleading practices observed in the labeling of these supplements.
Article
Biochemistry & Molecular Biology
Ana Pozo-Rodriguez, Juan A. A. Mendez-Liter, Rocio Garcia-Villalba, David Beltran, Eva Calvino, Andres G. Santana, Laura I. I. De Eugenio, Francisco Javier Canada, Alicia Prieto, Jorge Barriuso, Francisco A. A. Tomas-Barberan, Maria Jesus Martinez
Summary: Resveratrol was glycosylated by using rXynSOS-E236G glycosynthase, resulting in the production of 3-O-beta-D-xylobiosyl resveratrol as the major product. Response surface methodology was used to optimize the reaction, yielding 35% of 3-O-beta-D-xylobiosyl resveratrol. Xylobiosylation decreased the antioxidant capacity of resveratrol, but significantly improved its solubility for improved delivery and transit to the colon.
Review
Biochemistry & Molecular Biology
Tomislav Pejcic, Milica Zekovic, Uros Bumbasirevic, Milica Kalaba, Irena Vovk, Maja Bensa, Lazar Popovic, Zivoslav Tesic
Summary: This narrative review provides an overview of the epidemiological studies on breast cancer and prostate cancer, with a focus on the global incidence distribution and diet-related factors. It discusses the potential role of isoflavones in preventing these cancers by examining their biological properties, mechanisms of action, and available data. The review also summarizes studies on the effects of isoflavones in breast and prostate tissue, including their impact on cellular molecular signaling pathways and oxidative stress, as well as the levels of isoflavones in tissues and secretions.
Article
Agriculture, Multidisciplinary
Carlos E. Iglesias-Aguirre, Rocio Garcia-Villalba, David Beltran, Maria Dolores Frutos-Lison, Juan C. Espin, Francisco A. Tomas-Barberan, Maria V. Selma
Summary: We discovered a new bacterium in the feces of a healthy woman that can produce urolithins A and B as well as intermediate metabolites. Additionally, we developed co-culture systems that reproduced the production of urolithins upon fermentation of UM-A and UM-B. This finding demonstrates the potential of these urolithin-producing bacteria for use as probiotics and in the production of bioactive urolithins for various applications.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Microbiology
Malgorzata Wronkowska, Dorota Szawara-Nowak, Mariusz Konrad Piskula, Henryk Zielinski
Summary: The in vitro bioaccessibility of soluble proteins and Maillard reaction products (MRPs) in biscuits made from fermented buckwheat flours was studied. The results showed that the bioaccessibility of soluble proteins increased after digestion, and the content of furosine, a MRP indicator, was lower in the fermented biscuits. The FAST index, indicating the fluorescence of advanced MRPs, was almost twice as high in the biscuits fermented by specific strains of lactic acid bacteria. The study suggests that fermentation by lactic acid bacteria can enhance the bioaccessibility of MRPs in buckwheat products.
Article
Multidisciplinary Sciences
Kjersti Aaby, Mathias Rudolf Amundsen
Summary: The aim of this study was to investigate the effects of sweeteners (sucrose, acesulfame K, or sucralose) and temperature on the stability of phenolic compounds and color of lingonberry juice. The results showed that the addition of sweeteners had no impact on the stability of phenolic compounds or the color of lingonberry juice, while temperature significantly affected the stability of phenolic compounds. In general, after thermal treatment, the lingonberry juice became darker and bluer with lower chromaticity, while after storage, the juice became lighter, more yellow, and had higher chromaticity.
Article
Biochemistry & Molecular Biology
Nisa Beril Sen, Etil Guzelmeric, Irena Vovk, Vesna Glavnik, Hasan Kirmizibekmez, Erdem Yesilada
Summary: This study compared the chemical profiles, phenolic marker compounds, and pharmacological activities of ivy bee pollen samples from Turkey and Slovenia, as well as ivy flower pollen grains. The pollen samples from different locations had similar chemical profiles due to the same botanical origins. Marker compounds common to both bee pollen and flower pollen were isolated and their structures were elucidated. In vitro tests were conducted to evaluate the xanthine oxidase inhibition and antioxidant activity of the marker compounds and extracts.
Article
Microbiology
Ryan Sweet, Catherine Booth, Kathryn Gotts, Stephen F. Grove, Paul A. Kroon, Mark Webber
Summary: Antimicrobial resistance is a common problem in bacterial isolates, necessitating the development of effective novel antimicrobials. Phytochemicals, derived from plants, show promising antimicrobial activity. This study identified nine phytochemical compounds with consistent bioactivity against various pathogens.
Article
Agriculture, Multidisciplinary
Gabriela L. Salazar-Orbea, Rocio Garcia-Villalba, Maria J. Bernal, Alberto Hernandez-Jimenez, Jose A. Egea, Francisco A. Tomas-Barberan, Luis M. Sanchez-Siles
Summary: This research focused on how storage conditions and processing techniques affect the composition of nutrients, bioactive compounds, and sensory attributes in strawberry and apple purees. The study found that storage conditions had a stronger impact on phenolic compounds in strawberry puree, while initial processing techniques had a greater influence on apple puree. The stability of polyphenols varied between fruits during storage, and selecting the optimal storage conditions is crucial for maintaining the polyphenol content in sensitive fruits like strawberries.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)