Article
Energy & Fuels
Zakiullah Zaidi, Laxmi Gayatri Sorokhaibam
Summary: A novel chemical-biological approach using molybdenum and Rhodococcus erythropolis for liquid fuel desulfurization was investigated. The results show that Rh-Mo@FAT can significantly reduce the concentration of organosulfur compounds in fuel oil.
Article
Engineering, Chemical
George Prasoulas, Konstantinos Dimos, Panayiotis Glekas, Styliani Kalantzi, Stamatis Sarris, Chrysovalantis Templis, Konstantinos Vavitsas, Dimitris G. Hatzinikolaou, Nikolaos Papayannakos, Dimitris Kekos, Diomi Mamma
Summary: BDS using resting cells of Rhodococcus erythropolis IGTS8 was investigated in a bubble column bioreactor with two-phase media, finding that biocatalyst performance was best at an OFP of 50% v/v, and biocatalysts from the exponential growth phase exhibited the highest biodesulfurization efficiency. A new semi-empirical model based on Michaelis-Menten kinetics was developed to describe the biphasic BDS process.
Review
Biotechnology & Applied Microbiology
Jie Hou, Hong-Kuan Deng, Zi-Xin Liu, Ping Xu, Li-Juan Wang
Summary: This article introduces the sulfur metabolism mechanism of the well-known anaerobic bacteria Rhodococcus, including sulfur absorption, reduction, and assimilation, as well as the regulation mechanism and optimization strategies of the 4S pathway. The influence of sulfur metabolism on the efficiency of biodesulfurization is discussed, along with the latest genetic engineering strategies in Rhodococcus. A better understanding of the relationship between sulfur metabolism and desulfurization can promote the industrial application of biodesulfurization.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Energy & Fuels
Toru Matsui
Summary: Thianthrene can be degraded by Gordonia sp. TM414 and Rhodococcus erythropolis KA2-5-1, but not by R. jostii T09. The degradation pathway is similar to the 4S pathway with DBT. Thianthrene desulfurization is possible using Rhodococcus sp., which is closely related to Gordonia spp.
JOURNAL OF THE JAPAN PETROLEUM INSTITUTE
(2022)
Review
Biotechnology & Applied Microbiology
Abrar Ahmad, Mazin A. Zamzami, Varish Ahmad, Salwa Al-Thawadi, Mohammad Salman Akhtar, Mohd Jahir Khan
Summary: Deep hydrodesulfurization is costly and environmentally harmful, and fails to completely separate sulfur from heterocyclic poly-aromatic compounds. Various microorganisms have shown potential in removing sulfur from petroleum fractions via the 4S pathway, but activity levels up to 100 mu M/g dry cell weights are needed for effective desulfurization. This review discusses the desulfurization capabilities of different microorganisms and the potential for commercial application in the petroleum industry.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Valentine E. Anyanwu, Stephen J. Hall, Gill Stephens, Anca Pordea
Summary: This study demonstrates the potential of putrescine oxidase from Rhodococcus erythropolis for the bioproduction of N-heterocycles from cadaverine. The enzyme showed good activity under optimal temperature and pH conditions, with complete biotransformation of cadaverine observed in whole cells. A direct assay of enzyme activity for cadaverine oxidation was successfully implemented, confirming the efficiency of the enzyme for this biotechnological application.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Zachary J. Butz, Alexander Hendricks, Kanda Borgognoni, Christopher J. Ackerson
Summary: A Rhodococcus erythropolis bacterium was identified to tolerate normally lethal concentrations of various metal ions, with a mycothione reductase (Mtr) showing remarkable selectivity for TeO32- reduction. The selectivity for TeO32- is attributed to aromatic residues near the active site of the enzyme, resulting in a Te-0 enzymatic product resembling Te nanowires.
FEMS MICROBIOLOGY ECOLOGY
(2021)
Article
Environmental Sciences
Shu Ma, Shiwei Huang, Yun Tian, Xiangyang Lu
Summary: In this study, a strain of Rhodococcus erythropolis Y10 capable of aerobic denitrification was screened from surface flow constructed wetlands. The strain removed ammonium through heterotrophic ammonium assimilation and exhibited improved aerobic denitrification efficiency when ammonium was added. The findings shed light on the role of ammonium assimilation in microbial ammonium removal.
Article
Chemistry, Multidisciplinary
Maraylla I. Moraes, Cesar Iglesias, Iris S. Teixeira, Humberto M. S. Milagre, Sonia Rodriguez Giordano, Cintia D. F. Milagre
Summary: We report the cloning and expression of Rhodococcus erythropolis ATCC 4277 nitrile hydratase (alpha-and beta-subunits) and the correspondent activator gene. Furthermore, substrate scope with whole cells of recombinant E. coli demonstrates that this Fe-type NHase could hydrate a wide range of aliphatic and aromatic nitrile with high conversion rates and moderate enantiomeric excess.
RESULTS IN CHEMISTRY
(2023)
Article
Microbiology
Soon Dong Lee, In Seop Kim
Summary: These two novel actinobacterial strains isolated from soil samples of a cave in Jeju Island, Republic of Korea, were classified as Rhodococcus spelaei sp. nov. based on a combination of morphological, physiological, and genomic analyses. Furthermore, genome analysis revealed the heterotypic synonyms relationships within the genus Rhodococcus.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Edina Garai, Anita Risa, Emese Varga, Matyas Cserhati, Balazs Kriszt, Bela Urbanyi, Zsolt Csenki
Summary: The NI1 strain was able to degrade various mycotoxins and their mixtures in different proportions, reducing the toxic effects and transforming them into non-toxic degradation products. However, some mixtures, such as the AFB1+T-2 mixture, were degraded into more toxic products. Single exposures to mycotoxins were highly toxic, while combined exposures showed synergistic or antagonistic effects, depending on the specific mixture.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Energy & Fuels
Roselle Y. Mamuad, Angelo Earvin Sy Choi
Summary: The presence of elevated sulfur levels in diesel oil poses significant risks to human health, animals, the environment, vehicles, and infrastructure. Sulfur is a major contributor to particulate matter and emissions, and regulations regarding sulfur content in diesel fuel have become more stringent. Eco-friendly desulfurization techniques like biodesulfurization (BDS) are being explored, but further research is needed to fully understand the mechanism and make it more competitive in industrial applications. Combining BDS with other desulfurization technologies can potentially reduce costs and improve resource allocation.
Article
Microbiology
Selina Engelhart-Straub, Martina Haack, Dania Awad, Thomas Brueck, Norbert Mehlmer
Summary: This study investigates the substrate adaptability of the oleaginous bacterium Rhodococcus erythropolis and determines the optimum carbon and nitrogen concentrations for achieving the highest biomass, lipid, and carotenoid yields. These results provide insights for optimizing media composition in the production of valuable oleochemicals and pigments.
Article
Food Science & Technology
Vindya Thathsaranee Weligama Thuppahige, Lalehvash Moghaddam, Zachary G. Welsh, Tony Wang, Hong-Wei Xiao, Azharul Karim
Summary: This study investigated the potential of extracting starch from cassava peel and bagasse as non-edible sources. The study developed an extraction method to maximize starch extraction and compared the properties of the extracted starch with commercial cassava starch. The results showed that cassava peel starch had a significantly higher yield than bagasse starch, and the extracted starch from both sources exhibited similar morphology and functional groups.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Martin Peng, Dominik L. Siebert, Martin K. M. Engqvist, Christof M. Niemeyer, Kersten S. Rabe
Summary: A novel thermostable benzaldehyde lyase ReBAL was discovered, showing structural differences from PfBAL but with higher thermostability and different activity in rational design-generated variants. Using the most stable variant, (R)-benzoin was successfully synthesized, providing new insights for the production of alpha-hydroxy ketones and APIs.
Article
Business
Ana Julia Dal Forno, Walakis Vieira Bataglini, Fernanda Steffens, Antonio Augusto Ulson de Souza
Summary: This paper presents a diagnostic instrument for assessing the maturity of Industry 4.0 technologies in the textile and clothing sector. The methodology includes a literature review and the development of a questionnaire. The findings suggest that the industry is still in the early stages of implementing Industry 4.0. The developed diagnostic tool can be used for internal benchmarking and improving the company's Industry 4.0 maturity level.
JOURNAL OF FASHION MARKETING AND MANAGEMENT
(2023)
Review
Agriculture, Multidisciplinary
Renato Dias Matosinhos, Karina Cesca, Bruno Augusto Mattar Carciofi, Debora de Oliveira, Cristiano Jose de Andrade
Summary: This article critically discusses the current status and future trends of mannosylerythritol lipids as green pesticides and biostimulants for seed germination and plant growth. It highlights their potential to partially replace synthetic pesticides, stimulate plant growth, and reduce phytopathogen infections.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Biotechnology & Applied Microbiology
Fernanda Raquel Wust Schmitz, Karina Cesca, Alexsandra Valerio, Debora de Oliveira, Dachamir Hotza
Summary: Novel rapid methodologies involving colorimetric biosensors and gold nanoparticles have been developed for the detection of bacteria, specifically Pseudomonas aeruginosa. The biosensor relies on the color change of gold nanoparticles induced by NaCl after incubation with bacteria and aptamer binding. The optimized conditions for detection were found to be 5 μM aptamer concentration and 200 mM of NaCl. The detection limit for P. aeruginosa was 10(5) CFU mL(-1) by the naked eye and 10(4) CFU mL(-1) by UV-Vis spectrometry.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Mirian Cristina Feiten, Iasmin Morigi, Marco Di Luccio, Jose Vladimir Oliveira
Summary: The aim of this study was to investigate the treatment of Candida antarctica Lipase B (CalB) in supercritical carbon dioxide (SC-CO2) and liquefied petroleum gas (LPG), as well as its activity after high-pressure treatment. The results showed that CalB treated with SC-CO2 at 35 ℃, 75 bar for 1 hour and LPG at 65 ℃, 30 bar for 1 hour had residual specific hydrolytic activities of 132% and 142%, respectively. The enzyme treated at high pressure still maintained an activity above 100% after 20 days of storage at low temperatures. There was no significant difference in the residual activity loss of CalB treated with LPG and stored at different temperatures over time. However, a greater difference was observed between CalB treated with CO2 and flash-frozen in liquid nitrogen (-196 ℃) followed by storage in a freezer (-10 ℃) compared to CalB stored in a freezer at -10 ℃. These findings suggest the need for further studies on CalB and other enzymes under different pressurized fluids for industrial applications.
BIOTECHNOLOGY LETTERS
(2023)
Article
Engineering, Chemical
Gabriela N. Pereira, Karina Cesca, Maria Angelica F. Pereira, Carla Roana Monteiro Rudke, Otilia Monica Alves Borges, Anelise Leal Vieira Cubas, Eduardo Zanella, Boris U. Stambuk, Patricia Poletto, Debora de Oliveira
Summary: This study explored the use of non-thermal plasma as an alternative pretreatment technique for different lignocellulosic residues. The results showed that non-thermal plasma can effectively delignify the residues within a short period of time, with minimal impact on the morphology and chemical characteristics of the residues.
JOURNAL OF FOOD PROCESS ENGINEERING
(2023)
Review
Biotechnology & Applied Microbiology
Vanessa Kristine de Oliveira Schmidt, Grazielly Maria Didier de Vasconscelos, Renata Vicente, Jackelyne de Souza Carvalho, Isabela Karina Della-Flora, Lucas Degang, Debora de Oliveira, Cristiano Jose de Andrade
Summary: The global production of cassava is approximately 303 million tons, which results in the generation of 0.65 kg of solid residue and 25.3 l of wastewater per kg of processed cassava root. The composition of the wastewater varies depending on its source, with cassava flour production exhibiting higher levels of organic load and total cyanide compared to starch processing. The presence of cyanide in cassava residues poses a toxicity risk to humans and animals. Developing eco-friendly methods for the treatment or reuse of cassava wastewater is a promising yet challenging endeavor. This study aims to explore the production of biosurfactants using cassava wastewater as an alternative culture medium.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Vanessa Kristine de Oliveira Schmidt, Paulo Alexandre Durant Moraes, Karina Cesca, Luiz Paulo Soares Pereira, Lidiane Maria de Andrade, Maria Anita Mendes, Debora de Oliveira, Cristiano Jose de Andrade
Summary: This study investigated the effect of hydrophobic inducers on surfactin production by B. subtilis ATCC 6633 using cassava wastewater as a low-cost culture medium. The results showed that hydrophobic inducers can enhance the production of surfactin and induce the production of new surfactin homologues.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Food Science & Technology
Edipo da Silva Almeida, Mateus Antonio Knapp, Juliana De Gregori da Rocha, Dachamir Hotza, Debora de Oliveira
Summary: The study aimed to evaluate the effects of a multi-stage block freeze concentration (BFC) on the physicochemical properties of Morinda citrifolia leaf extract. The results showed that the BFC process resulted in significantly higher concentrations of phenolic compounds and increased antioxidant activity. The findings suggest that this non-thermal, sustainable, and low-cost processing method can produce concentrated fractions with potential applications in functional food development from agroindustrial wastes.
Review
Biotechnology & Applied Microbiology
Suzana F. F. Bazoti, Charline Bonatto, Thamarys Scapini, Aline Frumi Camargo, Helen Treichel, Debora de Oliveira
Summary: Over the years, numerous studies have focused on exploring lignocellulosic biomass to achieve high yields of various bioproducts and develop economically viable industrial processes. Levulinic acid (LA) has emerged as a top platform product derived from biomass due to its growing market potential, which attracts investor attention. LA is considered a versatile building block with applications in various industries such as fuels, pharmaceuticals, cosmetics, food additives, and solvents. The primary route for LA synthesis is the acid-catalyzed degradation of cellulose. Sustainable production of LA from lignocellulosic biomass has been extensively researched, along with challenges in the synthesis process and the utilization of residual biomass. This comprehensive review proposes strategies to optimize LA production, such as process intensification and the use of fruit residues.
BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR
(2023)
Article
Polymer Science
Yago Araujo Vieira, Rosana Oliveira Henriques, Danyelle Gurgel, Diana Hartmann, Ricardo Antonio Francisco Machado, Debora de Oliveira, Bruno Francisco Oechsler, Agenor Furigo Jr
Summary: This study evaluates the potential of polyacrylonitrile particles (PAN) as a support for laccase immobilization. The immobilization yield of laccase was significantly improved using chemically modified PAN particles. The PAN/laccase derivative showed higher activity and stability at low pH and appropriate temperature, indicating the potential of PAN as a new support for laccase immobilization.
JOURNAL OF APPLIED POLYMER SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Bruna P. Soares, Ana M. Ferreira, Marina Justi, Luiz Gustavo Goncalves Rodrigues, J. Vladimir Oliveira, Simao P. Pinho, Joao A. P. Coutinho
Summary: Anthocyanins from jucara fruits were extracted using biobased solvents, 1,2-alkanediols and glycerol ethers, through either pressurized liquid extraction (PLE) or ultrasound-assisted extraction (UAE). The optimized PLE conditions resulted in 23.1 mg(anthocyanins)center dot g(dry biomass)(-1), while the UAE conditions with 1,2-propanediol yielded 50 mg(anthocyanins)center dot g(dry biomass)(-1). The biobased solvent solutions exhibited higher anthocyanin yields and antioxidant capacity compared to water and ethanolic extracts.
Article
Chemistry, Analytical
Lucas Veras, Florencio Diniz-Sousa, Giorjines Boppre, Vitor Devezas, Hugo Santos-Sousa, John Preto, Joao Paulo Vilas-Boas, Leandro Machado, Jose Oliveira, Helder Fonseca
Summary: The purpose of this study was to develop prediction equations for peak ground reaction force (pGRF) and peak loading rate (pLR) in high-impact activities for adults with various body masses. Regression equations were developed using accelerometry data, with body mass and peak acceleration or acceleration rate as predictors. The equations had high accuracy for predicting pGRF, with a coefficient of determination (R-2) of at least 0.83 and a mean absolute percentage error (MAPE) below 14.5%. However, the accuracy of the pLR prediction equations was lower than that of the pGRF equations.
Article
Environmental Sciences
Ruan F. F. Vieira, Eduardo B. B. Sydney, Karina G. G. Fiametti, Joao H. C. Wancura, J. Vladimir Oliveira
Summary: This study investigated the production of volatile fatty acids (VFAs) by a consortium of microorganisms obtained from whey. The effectiveness of chemical hydrolysis and supplementation with yeast extract and peptone was evaluated to determine the process that provides the highest VFAs production. The results showed that chemical hydrolysis did not significantly affect the production of compounds, while supplementation played a crucial role in increasing VFAs production. Butyric acid was the compound with the highest amount produced, and it had better results with supplemented hydrolyzed whey.
WASTE AND BIOMASS VALORIZATION
(2023)
Article
Engineering, Environmental
Felipe Matheus Muller, Debora de Oliveira, Camila Michels
Summary: The consumption of products of animal origin (PAO) has increased significantly in the past 50 years, leading to a rise in the generation of animal by-products' residues during processing. The rendering industry is the main method for adding value to these residues, resulting in a greater amount of raw material and the generation of gaseous effluents and wastewater. However, there is currently a lack of literature on rendering wastewater treatments and valorisation processes.
JOURNAL OF WATER PROCESS ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Suzana F. Bazoti, Aline Frumi Camargo, Charline Bonatto, Simone Kubeneck, Helen Treichel, Debora de Oliveira
Summary: This study proposes the synthesis of levulinic acid from watermelon residues, adding value to a residual biomass. The experiments were optimized using acid hydrolysis and a central composite design, with microwave-assisted synthesis showing higher yields. This study contributes to an energy-saving strategy and a circular carbon economy based on agricultural waste.
BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR
(2023)
