Review
Biochemistry & Molecular Biology
Jian Qin, Jian Xu
Summary: Arginine methylation plays a critical role in regulating EMT, affecting signaling, transcription, and splicing regulation.
Article
Gastroenterology & Hepatology
Ming Zhang, Can Chen, Zequn Lu, Yimin Cai, Yanmin Li, Fuwei Zhang, Yizhuo Liu, Shuoni Chen, Heng Zhang, Shuhui Yang, Hui Gen, Yuan Jiang, Caibo Ning, Jinyu Huang, Wenzhuo Wang, Linyun Fan, Yi Zhang, Meng Jin, Jinxin Han, Zhen Xiong, Ming Cai, Jiuyang Liu, Chaoqun Huang, Xiaojun Yang, Bin Xu, Heng Li, Bin Li, Xu Zhu, Yongchang Wei, Ying Zhu, Jianbo Tian, Xiaoping Miao
Summary: This study provides a comprehensive functional analysis of splicing quantitative trait loci (sQTLs) in cancer and specifically in colorectal cancer (CRC). The researchers identified genetic variants that control messenger RNA splicing and examined their association with CRC risk. They also conducted biological experiments to investigate the potential mechanisms of these sQTLs and target genes. The study highlights the role of sQTLs in cancer susceptibility and suggests their potential as biomarkers and therapeutic targets.
Article
Biochemistry & Molecular Biology
Qi Chen, Meng Gu, Zhi-kang Cai, Hu Zhao, Shi-cheng Sun, Chong Liu, Ming Zhan, Yan-bo Chen, Zhong Wang
Summary: CD44 serves as a marker of cancer stem cells in many tumors, with alternative splicing generating various isoforms. This study reveals a novel mechanism where TGF-beta 1 induces CD44 alternative splicing, promoting epithelial-to-mesenchymal transition and stemness in prostate cancer cells. The findings demonstrate the important roles of CD44 isoforms and TGF-beta 1 in tumor progression.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Review
Anatomy & Morphology
Jingyi Lyu, Chonghui Cheng
Summary: Alternative splicing is a critical mechanism of gene regulation that contributes to protein diversity in higher eukaryotes. Recent studies have demonstrated its role in regulating epithelial-mesenchymal transition (EMT) and associated activities. This review summarizes the functional impact of alternative splicing in EMT and discusses the regulatory mechanisms controlling splicing changes during EMT.
CELLS TISSUES ORGANS
(2022)
Review
Genetics & Heredity
Ling Li, Jinxia Zheng, Sebastian Oltean
Summary: This article discusses the role of alternative splicing (AS) in the regulation of epithelial-mesenchymal transition (EMT) and suggests that reversing or inhibiting EMT may provide new therapeutic strategies for suppressing tumor cell migration and metastasis.
Article
Multidisciplinary Sciences
Wen-juan Li, Yao-hui He, Jing-jing Yang, Guo-sheng Hu, Yi-an Lin, Ting Ran, Bing-ling Peng, Bing-lan Xie, Ming-feng Huang, Xiang Gao, Hai-hua Huang, Helen He Zhu, Feng Ye, Wen Liu
Summary: The study identified numerous substrates for Type I and II arginine methyltransferases and revealed features of PRMT7-regulated methylation, showing it primarily methylates a specific glycine and arginine motif. The study also demonstrated the role of PRMT4/5/7 in cancer-associated splicing regulation and potential as targets for cancer therapy. Pharmacological inhibition of PRMT4/5/7 was shown to suppress cancer cell growth with synergistic effects when co-inhibited.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Nguyen-Tuong Ho, Shu-Wei Lin, Yi-Rong Lee, Chii-Ruey Tzeng, Shu-Huei Kao
Summary: OPNb and OPNc are overexpressed in endometriosis and adenomyosis, and CD44v and the epithelial-mesenchymal transition (EMT) process are also upregulated. Overexpression of OPNb and OPNc splice variants leads to morphological changes, actin remodeling, cell proliferation, cell migration, and EMT through binding with CD44 and alpha v beta 3, subsequently activating the PI3K and NF-kappa B pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Iori Motoo, Sohachi Nanjo, Takayuki Ando, Satoshi Yamashita, Toshikazu Ushijima, Ichiro Yasuda
Summary: The study reveals that in diffuse-type gastric cancers, ULK2 is methylated and knockdown of ULK2 can induce autophagy, cell migration, and EMT. mRNA microarray analysis showed that knockdown of ULK2 altered the expressions of oncogenic genes associated with cell migration and EMT.
Article
Biochemical Research Methods
Yushan Qiu, Wai-Ki Ching, Quan Zou
Summary: The study introduces a novel method called WDFSMF for predicting RBP-AS target associations, which shows higher accuracy compared with previous methods. The method can select and integrate data sources, identify significant RBP complexes regulating AS events, and eliminate noise and outliers from the data.
BRIEFINGS IN BIOINFORMATICS
(2021)
Article
Oncology
Cheemala Ashok, Neha Ahuja, Subhashis Natua, Jharna Mishra, Atul Samaiya, Sanjeev Shukla
Summary: ESRP1 plays a significant role in cancer progression, with elevated expression in carcinoma in situ and reduced expression in hypoxic cancer cells. The transcription factor E2F1 and CpG hydroxymethylation are key factors in upregulating ESRP1 expression, while hypoxia-induced TET3 downregulation leads to E2F1 repression, contributing to diminished ESRP1 expression in hypoxic conditions.
Article
Multidisciplinary Sciences
Phuong Thao Ly, Shaohai Xu, Melissa Wirawan, Dahai Luo, Xavier Roca
Summary: The study demonstrates that different isoforms of ZAP play overlapping yet different roles in antiviral defense and other cellular processes. The regulation of host mRNA by ZAP is mostly unknown, but splice-switching antisense oligonucleotides can modulate the balance between ZAP isoforms. The study also identifies new cellular transcripts and processes regulated by ZAP isoforms, such as unfolded protein response, epithelial-mesenchymal transition, and innate immunity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Horticulture
Ding-Ding Zuo, Guang-Qi He, Hao-Ting Sun, Da-Long Guo
Summary: Hydrogen peroxide, methylation and alternative splicing have important regulatory roles in fruit development. This study analyzed the alternative splicing events during grape berry development under hydrogen peroxide treatment and control conditions. It was found that there were stage-specific regulations of alternative splicing and more events occurred at the early stages of berry development. Additionally, there were significant differences in alternative splicing events between the treatment and control, with some genes associated with methylation modifications.
SCIENTIA HORTICULTURAE
(2023)
Article
Chemistry, Multidisciplinary
Shangbiao Li, Yanwen Chen, Yuxin Xie, Hongchao Zhan, Yu Zeng, Kunlin Zeng, Li Wang, Ziling Zhan, Cuiying Li, Liqian Zhao, Xiaoxia Chen, Yujing Tan, Zhongyong Wang, Junguo Bu, Ye Song, Fan Deng, Aidong Zhou
Summary: The study reveals that FBXO7 confers mesenchymal properties and chemoresistance in glioblastoma. FBXO7 upregulation in mesenchymal glioblastoma stabilizes Rbfox2 through K63-linked ubiquitination after its arginine dimethylation by PRMT5, and thus controls Rbfox2-mediated splicing of mesenchymal genes, resulting in glioblastoma stem cell self-renewal, mesenchymal transformation, and chemoresistance. Targeting the FBXO7-Rbfox2 axis represents a potential strategy for glioblastoma treatment.
Article
Biochemistry & Molecular Biology
Tiantian Liu, Pengli Xu, Shaorui Ke, Haoran Dong, Mengmeng Zhan, Qin Hu, Jiansheng Li
Summary: SETDB1 regulates Snai1 expression epigenetically, driving EMT gene reprogramming and ferroptosis in response to TGF-beta. However, the links between epigenetic reprogramming and transcriptional processes in TGF-beta-dependent EMT regulation remain unexplored.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Multidisciplinary Sciences
Lei Huang, Xiao-Ou Zhang, Esteban J. Rozen, Xiaomei Sun, Benjamin Sallis, Odette Verdejo-Torres, Kim Wigglesworth, Daniel Moon, Tingting Huang, John P. Cavaretta, Gang Wang, Lei Zhang, Jason M. Shohet, Mary M. Lee, Qiong Wu
Summary: The protein arginine methyltransferase 5 (PRMT5) regulates the expression of metastasis-related genes by methylating Akt protein, which is required for its phosphorylation and activation, leading to the upregulation of pro-metastatic transcription factors. Moreover, inhibiting PRMT5 can attenuate primary tumor growth and block metastasis in multiple organs, suggesting it may be a potential therapeutic target for high-risk metastatic cancers.
NATURE COMMUNICATIONS
(2022)
Article
Oncology
George Vartholomatos, Haralambos Harissis, Maria Andreou, Vissaria Tatsi, Lamprini Pappa, Sevasti Kamina, Anna Batistatou, Georgios S. Markopoulos, George A. Alexiou
Summary: Evaluating tumor margin status during surgery is critical to prevent tumor recurrence. Intraoperative flow cytometry (iFC) showed greater sensitivity and similar specificity and accuracy compared to cytology in breast-conserving surgeries. The data supports the utility of iFC for rapid evaluation of excision margins, improving the precision of breast-conserving surgeries.
CLINICAL BREAST CANCER
(2021)
Article
Cell Biology
Eugenia Roupakia, Georgios S. Markopoulos, Evangelos Kolettas
Summary: Cellular senescence is a stable and irreversible cell cycle arrest triggered by various stimuli, including telomere shortening and oxidative, genotoxic, or oncogenic stresses. Effector mechanisms such as DNA damage responses, senescence-associated secretory phenotype, and epigenetic changes play important roles in senescence programmes. Despite its role as a cancer barrier, bypassing senescence may lead to the development of malignancies. Functional genetic screens have identified genes, pathways, and mechanisms involved in senescence evasion, including cell cycle regulators, DNA damage response pathways, and noncoding RNAs.
MECHANISMS OF AGEING AND DEVELOPMENT
(2021)
Editorial Material
Oncology
Georgios S. Markopoulos, Haralambos Harissis, Maria Andreou, George A. Alexiou, George Vartholomatos
SURGICAL ONCOLOGY-OXFORD
(2022)
Letter
Oncology
George Vartholomatos, George A. Alexou, Vissaria Tatsi, Haralambos Harissis, Georgios S. Markopoulos
Article
Biochemistry & Molecular Biology
Evrysthenis Vartholomatos, Stefania Mantziou, George A. Alexiou, Diamanto Lazari, Chrissa Sioka, Athanassios Kyritsis, Georgios S. Markopoulos
Summary: The study highlights the importance of natural substances as potential antiglioma agents and their targeting of CD markers on glioma cells. The identified NF-kappa B binding sites and core regulatory network provide potential targets for glioblastoma treatment.
Article
Surgery
K. Vaia Georvasili, S. Georgios Markopoulos, Anna Batistatou, Michael Mitsis, Thomas Messinis, D. Georgios Lianos, George Alexiou, George Vartholomatos, D. Christina Bali
Summary: The study introduces an iFC protocol for colorectal cancer detection, with a high accuracy in predicting the presence of cancer cells based on the tumor-index and good accuracy in tumor margin evaluation for rectal cancer patients.
INTERNATIONAL JOURNAL OF SURGERY
(2022)
Review
Oncology
Michail Mitsis, Panagiota Drosou, Vasileios Tatsis, Georgios S. Markopoulos
Summary: This study summarizes the role of neutrophil extracellular traps (NETs) in the development of pancreatic adenocarcinoma (PDAC). The results suggest that NETs have a dual role in PDAC progression and metastasis, promoting cancer cell proliferation and invasion while also potentially leading to cancer-associated thrombosis. The interplay between inflammation and cancer progression may be involved in these mechanisms.
Article
Oncology
Spyros Foutadakis, Eugenia Roupakia, Panagiotis Liakopoulos, Petros Kolovos, Evangelos Kolettas
Summary: Transcription Factors (TFs) are key regulators of gene expression, controlling aspects such as cell homeostasis, identity, and fate. NF-kappa B and E2F1 are important TFs involved in inflammation and cell cycle progression, respectively. This study compared their genomic binding profiles and found distinct preferences in the type of DNA elements they bind to. Additionally, NF-kappa B and E2F1 showed limited overlap in their binding sites and tended to bind to active chromatin even before stimulation. Furthermore, NF-kappa B was found to recruit E2F1 near pro-inflammatory genes following immune stimulation.
Article
Biology
Zoi Anastasiadi, Stefania Mantziou, Christos Akrivis, Minas Paschopoulos, Eufemia Balasi, Georgios D. Lianos, George A. Alexiou, Michail Mitsis, George Vartholomatos, Georgios S. Markopoulos
Summary: In this study, the role of intraoperative flow cytometry in gynecological malignancies was investigated. The results confirmed its value in cancer cell identification and suggested the need for further validation in larger multicenter studies. Cell-cycle analysis showed a correlation between aneuploidy and poor prognosis in intraoperative tumor characterization.
Article
Oncology
Athanasios Paliouras, Georgios S. Markopoulos, Stavros Tsampalas, Stefania Mantziou, Ioannis Giannakis, Dimitrios Baltogiannis, Georgios K. Glantzounis, George A. Alexiou, Evangelia Lampri, Nikolaos Sofikitis, George Vartholomatos
Summary: Bladder cancer predominantly affects male patients, with surgical treatment being the primary option. Intraoperative flow cytometry (iFC) is highly specific, sensitive, and accurate in detecting cancer cells, based on cell proliferation and tumor aneuploidy. Further research on iFC as a novel malignancy evaluation tool during transurethral resections is supported.
Article
Biochemistry & Molecular Biology
Maria Ntinopoulou, Dimitrios Cassimos, Eugenia Roupakia, Evangelos Kolettas, Maria Panopoulou, Elpis Mantadakis, Theocharis Konstantinidis, Akrivi Chrysanthopoulou
Summary: Elevated levels of neutrophil extracellular traps (NETs) and interleukin (IL)-17A were found in the sera of children during asthma exacerbation. The stimulation of control neutrophils with these sera resulted in IL-17A-enriched NET formation, which led to fibroblasts acquiring a pre-fibrotic phenotype and promoting fibrosis.
Article
Medicine, Research & Experimental
Georgio S. S. Markopoulos
Summary: This study systematically catalogued the regulatory elements within HERVs and found that they are widely present in the human genome and participate in various functions such as inflammation, chemokine signaling, and response to viral infection.
Meeting Abstract
Transplantation
Anila Duni, Lampros Lakkas, Georgios Markopoulos, Aris Bechlioulis, Vasileios Koutlas, Eirini Tzalavra, Vasileios Tatsis, Ioanna Theodorou, Haralambos Pappas, George Vartholomatos, Michael Mitsis, Katerina Naka, Evangelia Ntounousi
NEPHROLOGY DIALYSIS TRANSPLANTATION
(2022)
Meeting Abstract
Transplantation
Anila Duni, Lampros Lakkas, Aris Bechlioulis, Georgios Markopoulos, Revekka Georgiou, John Alekos, Athanasios Kitsos, Olga Balafa, Ioanna Theodorou, George Vartholomatos, Michael Mitsis, Katerina Naka, Evangelia Ntounousi
NEPHROLOGY DIALYSIS TRANSPLANTATION
(2022)
Meeting Abstract
Transplantation
Anila Duni, Ioannis Mallioras, Georgios Markopoulos, Haralambos Pappas, Efthymios Pappas, Vasileios Koutlas, Eirini Tzalavra, Gerasimos Baxevanos, Silvia Priska, Georgios Katagis, Konstantina Gartzonika, George Vartholomatos, Mixalis Mitsis, Evangelia Ntounousi
NEPHROLOGY DIALYSIS TRANSPLANTATION
(2022)