Article
Biochemistry & Molecular Biology
Hao Li, Luqi Li, Xun Qiu, Jing Zhang, Zichun Hua
Summary: CFLAR interacts with p130Cas to promote tumor growth and migration, and their high expressions are associated with poor prognosis in lung cancer patients.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2023)
Review
Developmental Biology
Naoya Yamaguchi, Holger Knaut
Summary: Cell-extracellular matrix interactions are essential for cell anchoring and migration, with focal adhesions (FAs) playing a key role. However, the role of FAs in vivo is still not well understood.
Article
Microbiology
Courtney M. Klappenbach, Nicholas M. Negretti, Jesse Aaron, Teng-Leong Chew, Michael E. Konkel
Summary: Our study reveals that C. jejuni infection alters the size and topology of focal adhesions in epithelial cells, leading to changes in cell adhesion strength and motility. The increased phosphorylation of paxillin and altered turnover at focal adhesions may contribute to the effects on cell behavior. Additionally, inhibiting protein synthesis or using specific mutants of C. jejuni can partially reverse these effects, suggesting a potential mechanism for the observed restricted intestinal repair in infected animals.
Article
Multidisciplinary Sciences
Jana Slovakova, Mateusz Sikora, Feyza Nur Arslan, Silvia Caballero-Mancebo, S. F. Gabriel Krens, Walter A. Kaufmann, Jack Merrin, Carl-Philipp Heisenberg
Summary: The tension of the actomyosin cell cortex is crucial in determining the growth and size of cell-cell contact. This relationship increases proportionally within a certain range of tension, but inversely scales above a critical threshold. This switch is caused by cortical tension promoting the anchoring of E-cadherin to the actomyosin cytoskeleton, thereby increasing clustering and stability of E-cadherin at the contact.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Yu-Shan Lin, Yi-Hsin Lin, MyHang Nguyen Thi, Shih-Chuan Hsiao, Wen-Tai Chiu
Summary: The dysregulation of store-operated Ca2+ entry (SOCE) is associated with cancer progression. Upregulation of stromal interaction molecule 1 (STIM1) promotes cancer cell migration, invasion, and metastasis, while excessive Ca2+ can have a negative impact on cancer metastasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Materials Science, Biomaterials
Carolin Grandy, Fabian Port, Jonas Pfeil, Mariana Azevedo Gonzalez Oliva, Massimo Vassalli, Kay-Eberhard Gottschalk
Summary: Cells are connected to the extracellular matrix through the focal adhesion complex, which also acts as a force sensor. However, the effect of tension on the structure of focal adhesions is still poorly understood. In this study, the location of key focal adhesion proteins, including vinculin, paxillin, and actin, was analyzed under tension using micropatterning and metal-induced energy transfer measurements. The results show that actin is particularly affected by the force balance, and blocking mechanosensitive ion channels significantly alters the architecture of focal adhesions and the distribution of these proteins.
BIOMATERIALS ADVANCES
(2023)
Article
Cell Biology
Xinyi Ren, Xiaopu Guo, Zihan Liang, Renxian Guo, Shaohui Liang, Han Liu
Summary: Cell migration is a complex process that involves the coordination of various cellular components. In this study, we discovered a novel interaction between IQGAP1 and Hax1, which plays a significant role in focal adhesion turnover and directional cell migration. Our findings suggest that Hax1 acts as a link between dynamic focal adhesions and IQGAP1, facilitating efficient cell migration. Perturbation of the IQGAP1/Hax1 interaction impairs focal adhesion turnover and inhibits directional cell migration.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Oncology
Alexander Y. Deneka, Meghan C. Kopp, Anna S. Nikonova, Anna Gaponova, Anna A. Kiseleva, Harvey H. Hensley, Douglas B. Flieder, Ilya G. Serebriiskii, Erica A. Golemis
Summary: This study reveals a new role for the scaffolding protein NEDD9 in regulating LKB1-AMPK signaling in early stage NSCLC, suppressing autophagy and tumor growth.
Article
Cell Biology
Elizabeth Riffo, Mario Palma, Matias Hepp, Diego Benitez-Riquelme, Vicente A. Torres, Ariel F. Castro, Roxana Pincheira
Summary: SALL2/Sall2 is a transcription factor associated with development, neuronal differentiation, and cancer. Sall2 promotes cell migration by modulating focal adhesion dynamics and transcriptionally regulating integrin beta 1 expression and FAK autophosphorylation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Elizabeth Riffo, Mario Palma, Matias Hepp, Diego Benitez-Riquelme, Vicente A. Torres, Ariel F. Castro, Roxana Pincheira
Summary: SALL2/Sall2 is a transcription factor that plays a positive role in cell migration, and its deficiency can lead to decreased cell motility and altered focal adhesion dynamics. Sall2 promotes cell migration by regulating integrin beta 1 expression and FAK autophosphorylation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Farnaz Hemmati, Ayuba Akinpelu, Jiyeon Song, Farshad Amiri, Anya McDaniel, Collins McMurray, Alexandros Afthinos, Stelios T. T. Andreadis, Andrew V. V. Aitken, Vinicia C. C. Biancardi, Sharon Gerecht, Panagiotis Mistriotis
Summary: Cell migration through confining 3D topographies can lead to detrimental effects such as loss of nuclear envelope integrity and DNA damage. However, cells exposed to long-term confinement do not usually die. This study utilizes photopatterning and microfluidics to develop a high-throughput device for prolonged culture of single cells in microchannels. The results show that continuous exposure to tight confinement can trigger nuclear envelope rupture events and promote cell apoptosis.
Article
Chemistry, Multidisciplinary
Setareh Sharifi Panah, Robert Grossmann, Valentino Lepro, Carsten Beta
Summary: This study explores the speed of cargo and the resulting drag force as the radius of the spherical cargo particle increases in a biohybrid cell-cargo system driven by amoeboid Dictyostelium discoideum cells. The findings suggest that there is a maximal cargo size beyond which the cell-driven movements will cease. Additionally, the study reveals that the cell-generated forces increase significantly when challenged by an external pulling force, showcasing the mechanoresponsive adaptation of this biohybrid system in complex environments.
Article
Developmental Biology
Hannah M. Olson, Alex V. Nechiporuk
Summary: Collective cell migration involves coordinated migratory behavior of cell cohorts. Protrusive behavior in the zebrafish posterior lateral line primordium (pLLP) is regulated by the Arp2/3 complex, leading to the formation of brush-like protrusions that aid in migration towards the overlying skin. Disruption of focal adhesion complexes surrounding the pLLP impairs migration, with Erk signaling playing a key role in the proper formation of protrusions and pLLP migration.
DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Jingduo Li, Xiupeng Zhang, Zaiyu Hou, Siqi Cai, Yingxue Guo, Limei Sun, Ailin Li, Qingchang Li, Enhua Wang, Yuan Miao
Summary: This study reveals the important role of P130cas in FAK-YAP axis-mediated radioresistance in non-small cell lung cancer (NSCLC). P130cas promotes cell proliferation, alters the cell cycle profile, and enhances tumor growth. Furthermore, the study demonstrates that P130cas interacts with YAP and promotes its activation and nuclear translocation.
CELL DEATH & DISEASE
(2022)
Review
Biochemistry & Molecular Biology
Jaime A. Espina, Cristian L. Marchant, Elias H. Barriga
Summary: Directed cell migration is crucial in physiological and pathological processes, influenced by both mechanical and chemical signals. Recent focus has been on the mechanical guidance of cell migration via durotaxis, with studies showing how cells sense and respond to environmental mechanics, leading to biased migration. Advances in understanding durotaxis in vivo and its integration with chemotaxis for haptotaxis in native environments have also been discussed.
Article
Medicine, Research & Experimental
Loretta M. S. Lau, Chelsea Mayoh, Jinhan Xie, Paulette Barahona, Karen L. MacKenzie, Marie Wong, Alvin Kamili, Maria Tsoli, Tim W. Failes, Amit Kumar, Emily V. A. Mould, Andrew Gifford, Shu-Oi Chow, Mark Pinese, Jamie Fletcher, Greg M. Arndt, Dong-Anh Khuong-Quang, Carol Wadham, Georgina Eden, Peter Trebilcock, Swapna Joshi, Stephanie Alfred, Anjana Gopalakrishnan, Aaminah Khan, Dylan Grebert Wade, Patrick A. Strong, Elodie Manouvrier, Lisa T. Morgan, Roxanne Cadiz, Caitlin Ung, David M. Thomas, Katherine M. Tucker, Meera Warby, Geoffrey B. McCowage, Luciano Dalla-Pozza, Jennifer A. Byrne, Federica Saletta, Andrew Fellowes, Stephen B. Fox, Murray D. Norris, Vanessa Tyrrell, Toby N. Trahair, Richard B. Lock, Mark J. Cowley, Paul G. Ekert, Michelle Haber, David S. Ziegler, Glenn M. Marshall
Summary: The study developed a precision medicine platform involving rapid high-throughput drug screening and patient-derived xenografting for high-risk pediatric cancer patients. Results showed that 70% of patients across the entire testing platform were able to find treatment options, with some patients experiencing clinical benefits after changes in treatment plans.
EMBO MOLECULAR MEDICINE
(2022)
Article
Cell Biology
Magdalena Prechova, Zuzana Adamova, Anna-Lena Schweizer, Miloslava Maninova, Andreas Bauer, Delf Kah, Samuel M. Meier-Menches, Gerhard Wiche, Ben Fabry, Martin Gregor
Summary: This study reveals the crucial role of plectin in supporting intercellular junctions through organizing cortical cytoskeletal networks in epithelial sheets. Deficiency of plectin leads to abnormal cytoarchitecture, elevated intracellular tension, and reduced intercellular cohesion, resulting in destabilization of tissues under mechanical stress.
JOURNAL OF CELL BIOLOGY
(2022)
Meeting Abstract
Oncology
Jordan Staunton, Pamela Ajuyah Robertson, Angela Harris, Chelsea Mayoh, Marie Wong, Megan Rumford, Noemi Fuentes-Bolanos, Mark Cowley, Loretta Lau, David S. Ziegler, Paulette Barahona, Neevika Manoharan
Article
Multidisciplinary Sciences
Kazunori Sunadome, Alek G. G. Erickson, Delf Kah, Ben Fabry, Csaba Adori, Polina Kameneva, Louis Faure, Shigeaki Kanatani, Marketa Kaucka, Ivar Dehnisch Ellstroem, Marketa Tesarova, Tomas Zikmund, Jozef Kaiser, Steven Edwards, Koichiro Maki, Taiji Adachi, Takuya Yamamoto, Kaj Fried, Igor Adameyko
Summary: The developing skeleton produces mechanical tension that guides the directional outgrowth of skeletal muscles. Formation of oriented myofibrils is crucial in musculoskeletal development. The mechanisms that control myocyte orientation and fusion for muscle directionality in adults are still unknown.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Pamela Ajuyah, Chelsea Mayoh, Loretta M. S. Lau, Paulette Barahona, Marie Wong, Hazel Chambers, Fatima Valdes-Mora, Akanksha Senapati, Andrew J. Gifford, Colleen D'Arcy, Jordan R. Hansford, Neevika Manoharan, Wayne Nicholls, Molly M. Williams, Paul J. Wood, Mark J. Cowley, Vanessa Tyrrell, Michelle Haber, Paul G. Ekert, David S. Ziegler, Dong-Anh Khuong-Quang
Summary: This study reports a new subtype of midline gliomas with similar features to DMG but lacking the H3K27M mutation. These tumors have recurrent mutations in ACVR1 or EGFR and high expression of EZHIP. They have a poor prognosis similar to H3K27M DMG and exhibit distinct transcriptome and methylome profiles. The study provides new insights into the mechanism and pathway regulation of these tumors, potentially opening new therapeutic avenues.
SCIENTIFIC REPORTS
(2023)
Article
Medicine, General & Internal
Noemi Auxiliadora Fuentes Bolanos, Bhavna Padhye, Macabe Daley, Jacqueline Hunter, Kate Hetherington, Meera Warby, Eliza Courtney, Judy Kirk, Sarah Josephi-Taylor, Yuyan Chen, Frank Alvaro, Kristine Barlow-Stewart, Marie Wong-Erasmus, Paulette Barahona, Pamela Ajuyah, Ann-Kristin Altekoester, Vanessa J. Tyrrell, Loretta M. S. Lau, Claire Wakefield, Dianne Sylvester, Katherine Tucker, Mark Pinese, Luciano Dalla Pozza, Tracey A. O'Brien
Summary: The PREDICT study aims to identify germline cancer predisposition (CP) in children with cancer through trio whole-genome sequencing (trio WGS) and evaluate its clinical utility and psychosocial impact. The study will assess heritability by analyzing biological parents and examine the psychosocial impact of trio sequencing. This research will help inform the integration of comprehensive testing into the standard care of childhood cancer patients at diagnosis.
Article
Genetics & Heredity
Chelsea Mayoh, Andrew J. Gifford, Rachael Terry, Loretta M. S. Lau, Marie Wong, Padmashree Rao, Tyler Shai-Hee, Federica Saletta, Dong-Anh Khuong-Quang, Vicky Qin, Marion K. Mateos, Deborah Meyran, Katherine E. Miller, Aysen Yuksel, Emily V. A. Mould, Rachel Bowen-James, Dinisha Govender, Akanksha Senapati, Nataliya Zhukova, Natacha Omer, Hetal Dholaria, Frank Alvaro, Heather Tapp, Yonatan Diamond, Luciano Dalla Pozza, Andrew S. Moore, Wayne Nicholls, Nicholas G. Gottardo, Geoffrey McCowage, Jordan R. Hansford, Seong-Lin Khaw, Paul J. Wood, Daniel Catchpoole, Catherine E. Cottrell, Elaine R. Mardis, Glenn M. Marshall, Vanessa Tyrrell, Michelle Haber, David S. Ziegler, Orazio Vittorio, Joseph A. Trapani, Mark J. Cowley, Paul J. Neeson, Paul G. Ekert
Summary: By combining immunohistochemistry, RNA sequencing, and whole-genome sequencing, we identified a novel 15-gene immune signature, IPASS, associated with CD8(+) T-cell infiltration in high-risk paediatric cancers. Using this signature, we estimated that up to 31% of high-risk cancers exhibit infiltrating T-cells. Furthermore, we found that PD-L1 protein expression is poorly correlated with PD-L1 RNA expression, and neoantigen load and TMB are not predictive of T-cell infiltration in paediatric cancers.
Article
Physics, Applied
Sebastian J. Mueller, Ben Fabry, Stephan Gekle
Summary: Bioprinting of living cells can lead to shape deformations, affecting cell survival and functionality. Extensional stresses occurring when cells leave the nozzle have been ignored. Lattice Boltzmann simulations and a finite-element based cell model were used to study cell deformation inside the nozzle and at its exit. Two simple methods were developed to predict maximum cell stress and approximate cell strains based on printing parameters.
PHYSICAL REVIEW APPLIED
(2023)
Review
Engineering, Biomedical
Daniela Lazaro-Pacheco, Mina Mohseni, Samuel Rudd, Justin Cooper-White, Timothy Patrick Holsgrove
Summary: Low back pain is a major cause of disability worldwide and puts a significant socioeconomic burden on healthcare systems. Intervertebral disc degeneration is a primary cause of lower back pain, and despite the development of regenerative therapies, there are currently no commercially available devices or therapies for disc regeneration. Various models for mechanical stimulation and preclinical evaluation have been developed, but challenges and compromises remain.
APL BIOENGINEERING
(2023)
Article
Oncology
Claire E. Wakefield, Kate Hetherington, Eden G. Robertson, Mark W. Donoghoe, Jacqueline D. Hunter, Janine Vetsch, Jonathan M. Marron, Katherine M. Tucker, Glenn M. Marshall, Alexander Broom, Michelle Haber, Vanessa Tyrrell, David Malkin, Loretta Lau, Marion K. Mateos, Tracey A. O'Brien, David S. Ziegler
Summary: Parents and patients rarely regretted participating in a childhood cancer precision medicine trial, even when their hopes were not fulfilled. Most participants found the trial beneficial and expressed high satisfaction.
BRITISH JOURNAL OF CANCER
(2023)
Article
Oncology
Jessica M. Gereis, Kate Hetherington, Eden G. Robertson, Rebecca Daly, Mark W. Donoghoe, David S. Ziegler, Glenn M. Marshall, Loretta M. S. Lau, Jonathan M. Marron, Claire E. Wakefield
Summary: This study investigated the understanding of parents and adolescent patients participating in a precision medicine clinical trial for childhood cancer. The majority of parents were satisfied with the information materials but had low actual understanding of precision medicine. The study suggests the need for improving the language and format of the materials and providing targeted information resources to enhance understanding of precision medicine.
Article
Oncology
Chelsea Mayoh, Jie Mao, Jinhan Xie, Gabor Tax, Shu-Oi Chow, Roxanne Cadiz, Karina Pazaky, Paulette Barahona, Pamela Ajuyah, Peter Trebilcock, Angela Malquori, Kate Gunther, Anica Avila, Doo Young Yun, Stephanie Alfred, Anjana Gopalakrishnan, Alvin Kamili, Marie Wong, Mark J. Cowley, Sophie Jessop, Loretta M. S. Lau, Toby N. Trahair, David S. Ziegler, Jamie I. Fletcher, Andrew J. Gifford, Maria Tsoli, Glenn M. Marshall, Michelle Haber, Vanessa Tyrrell, Timothy W. Failes, Greg M. Arndt, Richard B. Lock, Paul G. Ekert, M. Emmy M. Dolman
Summary: One-third of pediatric cancer patients enrolled in precision medicine programs do not receive therapeutic recommendations from molecular profiling. In order to find potential strategies for treating high-risk pediatric patients, a study conducted in vitro screening of 125 patient-derived samples against a library of 126 anticancer drugs. The results showed that high-throughput drug screening (HTS) can identify effective biomarker-driven therapeutic strategies for high-risk pediatric cancers.
Article
Health Care Sciences & Services
Rebecca Daly, Kate Hetherington, Emily Hazell, Bethany R. Wadling, Vanessa Tyrrell, Katherine M. Tucker, Glenn M. Marshall, David S. Ziegler, Loretta Lau, Toby N. Trahair, Tracey A. O'Brien, Kiri Collins, Andrew J. Gifford, Michelle Haber, Mark Pinese, David Malkin, Mark J. Cowley, Jonathan Karpelowsky, Donna Drew, Chris Jacobs, Claire E. Wakefield
Summary: Precision medicine programs aim to identify personalized treatments for children with cancer, but the various professional groups involved in delivering these programs are understudied. This study explored the experiences of professionals involved in Australia's first precision medicine trial for children with poor-prognosis cancer. The professionals expressed both complexity in their roles and positive views about the impact of precision medicine on their profession.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Meeting Abstract
Oncology
Nadine Azzam, Nicole Melong, Lissandra Tuzi, Lisa Pinto, Jamie I. Fletcher, Alvin Kamili, Biljana Dumevska, Loretta Lau, Jennifer A. Chan, Donna L. Senger, Stephanie A. Grover, Michelle Haber, David Malkin, Jason N. Berman
Meeting Abstract
Oncology
Lara Berklite, Andrew Gifford, Derrick Lian, Loretta Lau, Adam Nelson, Robert Lorsbach, Somak Roy, Ashish Kumar, Jennifer Picarsic
PEDIATRIC BLOOD & CANCER
(2023)
