Structural differences between yeast and mammalian microtubules revealed by cryo-EM

Insights into the Distinct Mechanisms of Action of Taxane and Non-Taxane Microtubule Stabilizers from Cryo-EM Structures

(2017) Elizabeth H. Kellogg et al.   JOURNAL OF MOLECULAR BIOLOGY

A Force-Induced Directional Switch of a Molecular Motor Enables Parallel Microtubule Bundle Formation

(2016) Maxim I. Molodtsov et al.   CELL

Graded Control of Microtubule Severing by Tubulin Glutamylation

(2016) Max L. Valenstein et al.   CELL

Mutations in Human Tubulin Proximal to the Kinesin-Binding Site Alter Dynamic Instability at Microtubule Plus- and Minus-Ends

(2016) Shih-Chieh Ti et al.   DEVELOPMENTAL CELL

Structural comparison of the Caenorhabditis elegans and human Ndc80 complexes bound to microtubules reveals distinct binding behavior

(2016) Elizabeth M. Wilson-Kubalek et al.   MOLECULAR BIOLOGY OF THE CELL

Mechanistic Origin of Microtubule Dynamic Instability and Its Modulation by EB Proteins

(2015) Rui Zhang et al.   CELL

Thirty years of search and capture: The complex simplicity of mitotic spindle assembly

(2015) Rebecca Heald et al.   JOURNAL OF CELL BIOLOGY

CTFFIND4: Fast and accurate defocus estimation from electron micrographs

(2015) Alexis Rohou et al.   JOURNAL OF STRUCTURAL BIOLOGY

A new protocol to accurately determine microtubule lattice seam location

(2015) Rui Zhang et al.   JOURNAL OF STRUCTURAL BIOLOGY

Atomic-accuracy models from 4.5-Å cryo-electron microscopy data with density-guided iterative local refinement

(2015) Frank DiMaio et al.   NATURE METHODS

Ring closure activates yeast γTuRC for species-specific microtubule nucleation

(2015) Justin M Kollman et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Ring closure activates yeast γTuRC for species-specific microtubule nucleation

(2015) Justin M Kollman et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

A mutation uncouples the tubulin conformational and GTPase cycles, revealing allosteric control of microtubule dynamics

(2015) Elisabeth A Geyer et al.   eLife

Tools for macromolecular model building and refinement into electron cryo-microscopy reconstructions

(2015) Alan Brown et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

High-Resolution Microtubule Structures Reveal the Structural Transitions in αβ-Tubulin upon GTP Hydrolysis

(2014) Gregory M. Alushin et al.   CELL

Asymmetric Friction of Nonmotor MAPs Can Lead to Their Directional Motion in Active Microtubule Networks

(2014) Scott Forth et al.   CELL

EB1 Accelerates Two Conformational Transitions Important for Microtubule Maturation and Dynamics

(2014) Sebastian P. Maurer et al.   CURRENT BIOLOGY

Stu2, the Budding Yeast XMAP215/Dis1 Homolog, Promotes Assembly of Yeast Microtubules by Increasing Growth Rate and Decreasing Catastrophe Frequency

(2014) Marija Podolski et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Mechanical design principles of a mitotic spindle

(2014) Jonathan J Ward et al.   eLife

Overexpression, purification, and functional analysis of recombinant human tubulin dimer

(2013) Itsushi Minoura et al.   FEBS LETTERS

Likelihood-based classification of cryo-EM images using FREALIGN

(2013) Dmitry Lyumkis et al.   JOURNAL OF STRUCTURAL BIOLOGY

Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM

(2013) Xueming Li et al.   NATURE METHODS

EBs Recognize a Nucleotide-Dependent Structural Cap at Growing Microtubule Ends

(2012) Sebastian P. Maurer et al.   CELL

One-step purification of assembly-competent tubulin from diverse eukaryotic sources

(2012) Per O. Widlund et al.   MOLECULAR BIOLOGY OF THE CELL

A TOG:  -tubulin Complex Structure Reveals Conformation-Based Mechanisms for a Microtubule Polymerase

(2012) P. Ayaz et al.   SCIENCE

+TIPs: SxIPping along microtubule ends

(2012) Praveen Kumar et al.   TRENDS IN CELL BIOLOGY

Design, Overexpression, and Purification of Polymerization-Blocked Yeast αβ-Tubulin Mutants

(2011) Vinu Johnson et al.   BIOCHEMISTRY

The Phenix software for automated determination of macromolecular structures

(2011) Paul D. Adams et al.   METHODS

GTP S microtubules mimic the growing microtubule end structure recognized by end-binding proteins (EBs)

(2011) S. P. Maurer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Features and development ofCoot

(2010) P. Emsley et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

The Ndc80 kinetochore complex forms oligomeric arrays along microtubules

(2010) Gregory M. Alushin et al.   NATURE

Structural Basis of Interprotofilament Interaction and Lateral Deformation of Microtubules

(2010) Haixin Sui et al.   STRUCTURE

Phosphoregulation of the budding yeast EB1 homologue Bim1p by Aurora/Ipl1p

(2009) Tomasz Zimniak et al.   JOURNAL OF CELL BIOLOGY

Appion: An integrated, database-driven pipeline to facilitate EM image processing

(2009) Gabriel C. Lander et al.   JOURNAL OF STRUCTURAL BIOLOGY

Microtubule assembly, organization and dynamics in axons and dendrites

(2009) Cecilia Conde et al.   NATURE REVIEWS NEUROSCIENCE

Tracking the ends: a dynamic protein network controls the fate of microtubule tips

(2008) Anna Akhmanova et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Mal3, the Schizosaccharomyces pombe homolog of EB1, changes the microtubule lattice

(2008) Amédée des Georges et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Detection of GTP-Tubulin Conformation in Vivo Reveals a Role for GTP Remnants in Microtubule Rescues

(2008) A. Dimitrov et al.   SCIENCE