Article
Biochemistry & Molecular Biology
Neil H. J. Cunningham, Imene B. Bouhlel, Paul T. Conduit
Summary: Centrosomes play a crucial role in organizing microtubules in animal cells. The process of centriole duplication, which is important for maintaining centrosome numbers, is still not fully understood. This study suggests that non-centrosomal cues influence the establishment of a single duplication site, as daughter centrioles preferentially assemble on the side of the mother centriole facing the nuclear envelope in Drosophila embryos.
Article
Biology
Andre Bras Goncalves, Sarah Kirstine Hasselbalch, Beinta Biskopsto Joensen, Sebastian Patzke, Pernille Martens, Signe Krogh Ohlsen, Mathieu Quinodoz, Konstantinos Nikopoulos, Reem Suleiman, Magnus Per Damso Jeppesen, Catja Weiss, Soren Tvorup Christensen, Carlo Rivolta, Jens S. Andersen, Pietro Farinelli, Lotte Bang Pedersen
Summary: CEP78 is a centrosomal protein involved in ciliogenesis and ciliary length control. Through interaction with other proteins like CEP350 and EDD1, CEP78 negatively regulates CP110 level to promote ciliogenesis downstream of CEP350. This discovery provides important insights into the role of CEP78 in cellular ciliogenesis.
Article
Cell Biology
Nina Schweizer, Jens Luders
Summary: Centrioles are microtubule-based structures that play crucial roles in centrosome assembly and cilia formation. Recent studies have shown that the gamma TuRC complex, traditionally known for microtubule nucleation, may also be involved in microtubule anchoring and centriole stabilization. These insights into the diverse activities of gamma TuRC at centrioles provide a better understanding of their functions.
JOURNAL OF CELL SCIENCE
(2021)
Article
Biology
Emma A. Hall, Dhivya Kumar, Suzanna L. Prosser, Patricia L. Yeyati, Vicente Herranz-Perez, Jose Manuel Garcia-Verdugo, Lorraine Rose, Lisa McKie, Daniel O. Dodd, Peter A. Tennant, Roly Megaw, Laura C. Murphy, Marisa F. Ferreira, Graeme Grimes, Lucy Williams, Tooba Quidwai, Laurence Pelletier, Jeremy F. Reiter, Pleasantine Mill
Summary: By generating mice lacking PCM1, this study reveals the important role of PCM1 and centriolar satellites in ciliogenesis, specifically in motile cilia on ependymal cells. The findings demonstrate that PCM1 is involved in efficient protein trafficking to and from centrioles, including the removal of CP110 and CEP97 to initiate ciliogenesis, and the threshold for ciliogenesis differs between cell types.
Article
Cell Biology
Ingrid Hoffmann
Summary: This review summarizes and discusses developments in understanding the first steps of centriole duplication and their regulation.
Article
Cell Biology
Tomer Avidor-Reiss, Luke Achinger, Rustem Uzbekov
Summary: Centrioles are subcellular organelles that are crucial for normal cell function and development. They are found in most animal cell types but are absent in egg cells and exhibit diverse characteristics in sperm cells. The precise role of centrioles in fertility and early embryo development remains unclear.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Marta Lovera, Jens Luders
Summary: Mutations in genes encoding centriolar or ciliary proteins cause ciliopathies. There are numerous disorders with clinical features similar to ciliopathies listed in the Human Phenotype Ontology database, but they do not involve defects in the centriole-cilium proteome. Defects in different cellular compartments may indirectly impair cilia and cause additional, nonciliopathy phenotypes.
TRENDS IN CELL BIOLOGY
(2021)
Review
Genetics & Heredity
Tomer Avidor-Reiss, Rustem Uzbekov
Summary: It has been found that human spermatozoon may provide a second atypical centriole to the zygote, challenging the previous assumption that only one centriole is provided. Detailed ultrastructural analysis of bovine early embryos has identified the existence of atypical centrioles, raising doubts about the single-centriole model. Therefore, a reassessment of the role of paternal centrioles in human development is necessary.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2023)
Review
Cell Biology
Thao P. Phan, Andrew J. Holland
Summary: This review explores different models on how centrosome dysfunction affects cortical development, emphasizing that centrosome defects reduce cell proliferation in the developing cortex by prolonging mitosis. It also discusses microcephaly mutations unrelated to centrosomes, such as those involved in DNA replication and repair.
GENES & DEVELOPMENT
(2021)
Review
Biochemistry & Molecular Biology
Anton Sergeevich Shakhov, Aleksandra Sergeevna Churkina, Anatoly Alekseevich Kotlobay, Irina Borisovna Alieva
Summary: This review summarizes information about the specific features of the centrosome and its relationship with the cell function of highly specialized cells. The participation of the centrosome in the functional activity of these cells, as well as its role as a universal regulatory and distribution center, controlling the dynamic morphology of animal cells, is discussed. The direct involvement of the centrosome in the performance of functions by specialized cells is also highlighted.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Tomohiko Iwano, Tomoaki Sobajima, Sen Takeda, Akihiro Harada, Shin-ichiro Yoshimura
Summary: Primary cilia are organelles that protrude from the cell surface and function in signal sensing and transduction. EHBP1L1, CD2AP, and CIN85 are involved in controlling ciliary length. EHBP1L1 is required for the localization of CD2AP/CIN85 to the ciliary sheath, and the EHBP1L1-CD2AP/CIN85 interaction is crucial for ciliary length regulation. EHBP1L1 and CD2AP/CIN85 also play a role in actin network remodeling around the basal body.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Review
Cell Biology
Dandan Ma, Fulin Wang, Junlin Teng, Ning Huang, Jianguo Chen
Summary: Centrosomes consist of centrioles surrounded by pericentriolar material. The mother and daughter centrioles in G1 phase can be distinguished by the localization of their appendages. Distal appendages are involved in ciliogenesis, while subdistal appendages have various functions including microtubule anchoring and mitotic spindle regulation. Mutations in genes encoding appendage proteins can lead to ciliopathies and developmental defects. This Review discusses recent advances in understanding the composition, assembly, and roles of centriolar appendages.
JOURNAL OF CELL SCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Fatma Mansour, Felix J. Boivin, Iman B. Shaheed, Markus Schueler, Kai M. Schmidt-Ott
Summary: The primary cilium is crucial for tissue homeostasis and organ development, and defects in cilia development can lead to ciliopathic disorders. DAPs, crucial proteins in primary cilia formation, play a vital role in ciliogenesis, and mutations in genes encoding these proteins can result in NPHP.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Abraham Nguyen, Sarah C. Goetz
Summary: TTBK2 is a key regulator of primary cilia assembly and stability. It plays a role in maintaining the stability of the ciliary axoneme and regulating the composition of centriolar satellites and basal body pools.
MOLECULAR BIOLOGY OF THE CELL
(2023)
Article
Biology
Noemie Gaudin, Paula Martin Gil, Meriem Boumendjel, Dmitry Ershov, Catherine Pioche-Durieu, Manon Bouix, Quentin Delobelle, Lucia Maniscalco, Than Bich Ngan Phan, Vincent Heyer, Bernardo Reina-San-Martin, Juliette Azimzadeh
Summary: Rotationally asymmetric molecular features have been discovered in human centrioles, which are crucial for primary ciliogenesis and ciliary signaling.
Article
Cell Biology
Abdelhalim Loukil, Manuela Zonca, Cosette Rebouissou, Veronique Baldin, Olivier Coux, Martine Biard-Piechaczyk, Jean-Marie Blanchard, Marion Peter
JOURNAL OF CELL SCIENCE
(2014)
Article
Multidisciplinary Sciences
Abdelhalim Loukil, Fanny Izard, Mariya Georgieva, Shaereh Mashayekhan, Jean-Marie Blanchard, Andrea Parmeggiani, Marion Peter
SCIENTIFIC REPORTS
(2016)
Article
Multidisciplinary Sciences
Abdelhalim Loukil, Chloe Barrington, Sarah C. Goetz
Summary: The study identified CSNK2A1 as a key regulator of ciliogenesis, modulating cilia trafficking and stability. Mutations in Csnk2a1 lead to longer cilia, structural defects, and abnormal accumulation of key proteins, associated with human genetic disorders. The findings provide insights into mechanisms involved in ciliary length regulation and shed light on the significance of cilia instability in human disease.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)