Article
Biochemistry & Molecular Biology
Annemiek Dickhout, Dawid M. Kaczor, Alexandra C. A. Heinzmann, Sanne L. N. Brouns, Johan W. M. Heemskerk, Marc A. M. J. van Zandvoort, Rory R. Koenen
Summary: After deposition on endothelial cells, CCL5 and CXCL4 are rapidly and actively internalized by the cells, primarily depending on dynamin, clathrin, and G protein-coupled receptors. The chemokines seem to be directed towards the nucleus, with internalization process not impacting leukocyte arrest.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Martha Gschwandtner, Anis N. Gammage, Claire Deligne, Linda F. M. Mies, Alissa Domaingo, Devardarssen Murdamoothoo, Thomas Loustau, Anja Schwenzer, Rupert Derler, Raphael Carapito, Manuel Koch, Matthias Moergelin, Gertraud Orend, Andreas J. Kungl, Kim S. Midwood
Summary: Bidirectional dialogue between cellular and non-cellular components of the tumor microenvironment (TME) drives cancer survival. The interplay between the matrix molecule tenascin-C (TNC) and chemokine CCL2 in HER2+ breast cancer was studied, revealing their distinct roles in subverting anti-tumoral immunity. TNC supported sustained CCL2 synthesis and facilitated an immune suppressive macrophage phenotype, while CCL2 did not impact macrophage survival/activation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Wanying Tang, Ming Zhang, Yu Wang, Dan Ma, Mi Hu, Yangkai Zhang, Huiling Lin, Weiwei Jiang, Yuxin Ouyang, Liping Jiang, Pingping He, Guojun Zhao, Xinping Ouyang
Summary: This study demonstrates the protective effect of IGF-1 on fatty acid-induced mitochondrial apoptosis in macrophages, providing a potential therapeutic strategy for the treatment of lipotoxicity.
MOLECULAR MEDICINE REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Bhumika Rajoria, Xihong Zhang, Douglas Yee
Summary: The Insulin-like Growth Factor (IGF) system has long been studied in breast cancer progression, but targeting it has not been successful due to the complexity and homology of its receptors. This study examined the metabolic phenotype of breast cancer cells upon stimulation with IGF-1 and insulin. The results showed that IGF-1R, but not IR, regulates ATP production, providing evidence of the relationship between metabolic dysfunction, cancer, and the IGF axis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Immunology
Teizo Yoshimura, Chunning Li, Yuze Wang, Akihiro Matsukawa
Summary: Breast cancer is a common cancer worldwide, and metastasis is the main cause of cancer-related death. Human monocyte chemoattractant protein-1 (MCP-1/CCL2) was identified as a chemotactic factor for monocytes, and its role in cancer development and progression was investigated. Studies showed positive correlations between MCP-1 production in tumors and TAM infiltration, as well as cancer progression. MCP-1 was found to promote breast cancer metastasis to the lung and brain, but not bone. The mechanisms of MCP-1 production in the breast cancer microenvironment were also reported.
CELLULAR & MOLECULAR IMMUNOLOGY
(2023)
Article
Hematology
Benoit Manfroi, Maria De Grandis, Jerome Moreaux, Sebastien Tabruyn, Jean-Francois Mayol, Melanie Quintero, Christian Righini, Nathalie Sturm, Michel Aurrand-Lions, Bertrand Huard
Summary: Tumor invasion induces inflammatory response, impacting tumorigenesis. DLBCL tumor cells express CCL5, recruiting blood monocytes. TAMs in DLBCL have a noncanonical gene signature, potentially explaining the lack of impact on DLBCL development.
Article
Materials Science, Biomaterials
Hui-Jie Zhang, Fu-Shu Li, Feng Wang, Han Wang, Tong-Chuan He, Russell R. Reid, Bai-Cheng He, Qingyou Xia
Summary: Silk sericin-based hydrogel, derived from transgenic platelet-derived growth factor (PDGF)-BB, effectively enhances osteogenesis and inhibits adipogenic differentiation. The cross-talk between Smad and Stat3 pathways is involved in the synergistic induction of osteogenic differentiation by BMP9 and PDGF-BB in mesenchymal stem cells (MSCs).
REGENERATIVE BIOMATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Jun-Way Chang, Shan-Chi Liu, Yen-You Lin, Xiu-Yuan He, Yi-Syuan Wu, Chen-Ming Su, Chun-Hao Tsai, Hsien-Te Chen, Yi-Ching Fong, Sung-Lin Hu, Chien-Chung Huang, Chih-Hsin Tang
Summary: This study found that nesfatin-1 expression is increased in the synovial tissue of RA patients, and it promotes inflammation by regulating CCL2 expression and monocyte migration. Nesfatin-1 also promotes M1 macrophage polarization and the expression of proinflammatory cytokines.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2023)
Review
Chemistry, Medicinal
Fatemeh Meshkini, Nahid Ramezani-Jolfaie, Saman Sargazi, Cain C. T. Clark, Sepideh Soltani
Summary: A systematic review of the effects of lycopene on circulating IGF-1 and IGFBPs in adults found no consistent modifying effect of lycopene supplementation on IGF-1 and IGFBP levels. More research is needed to explore the effect of lycopene on the IGF-1 system.
PHYTOTHERAPY RESEARCH
(2022)
Review
Cell Biology
Sipin Zhu, Mei Liu, Samuel Bennett, Ziyi Wang, Kevin D. G. Pfleger, Jiake Xu
Summary: CCL2 is an inflammatory mediator that recruits immune cells and plays a crucial role in skeletal biology and diseases. Its interaction with CCR2 activates multiple signaling cascades affecting the skeletal system.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Oncology
Baoyue Pan, Ting Wan, Yun Zhou, Shuting Huang, Linjing Yuan, Yinan Jiang, Xiaojing Zheng, Pingping Liu, Huiling Xiang, Mingxiu Ju, Rongzhen Luo, Weihua Jia, Chunyan Lan, Jundong Li, Min Zheng
Summary: MYBL2/CCL2 axis plays a crucial role in TAMs recruitment and M2-like polarization in ovarian cancer, leading to immune evasion and anti-PD-1 resistance.
CANCER CELL INTERNATIONAL
(2023)
Article
Medical Laboratory Technology
Jason K. Y. Lee, Kendall Cradic, Ravinder J. Singh, JoAnna Jones, Jieli Li
Summary: This study compared the measurement of IGF-1 on four different platforms and found significant variations between them. These variations should be considered when managing patients with pituitary disorders.
CLINICA CHIMICA ACTA
(2023)
Review
Endocrinology & Metabolism
Wentao Zhong, Xueqing Wang, Yufei Wang, Guoqian Sun, Jia Zhang, Zhuo Li
Summary: Obesity is a global epidemic and a risk factor for endocrine-related cancers. It affects insulin, IGF-1, sex hormones, IGFBPs, and adipokines, with IGF-1 playing a crucial role in obesity-related endocrine cancers. This review discusses the impact of obesity on prostate, breast, and pancreatic cancers, focusing on the mechanism of IGF-1 and its interaction with estrogen and adipokines. Additionally, the current status of IGF-1R inhibitors in clinical practice and their potential combination with other anticancer drugs are briefly introduced.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Oncology
Konan J. W. Kanga, Patricia Mendonca, Karam F. A. Soliman, Dominique T. Ferguson, Selina F. Darling-Reed
Summary: Diallyl trisulfide (DATS) has shown inhibition of triple negative breast cancer (TNBC) cells by reducing expression of CCL2, indicating its potential role in therapy and prevention of TNBC.
ANTICANCER RESEARCH
(2021)
Article
Medicine, General & Internal
Barbara Wlodarczyk, Lukasz Durko, Przemyslaw Wlodarczyk, Renata Talar-Wojnarowska, Ewa Malecka-Wojciesko
Summary: Differentiating between pancreatic ductal adenocarcinoma (PDAC) and chronic pancreatitis (CP) poses a challenge. This study assessed the usability of IGF-1, IGFBP-2, and IGF-1/IGFBP-2 ratio in differentiating PDAC and CP. The results indicate that CA 19-9 demonstrates high potential as a marker for PDAC and CP differentiation.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Orthopedics
Rit Apinyankul, Katherine Hwang, Nicole Alexandriadria Segovia, Derek F. Amanatullah, James I. Huddleston, William J. Maloney, Stuart B. Goodman
Summary: Revision of both femoral and tibial components of a total knee arthroplasty (TKA) for aseptic loosening has favorable outcomes. Revision of only one loose component with retention of others has shorter operative time and lower cost; however, the implant survivorship and clinical outcomes of these different operations are unclear.
JOURNAL OF ARTHROPLASTY
(2023)
Article
Orthopedics
Junichi Kushioka, Masakazu Toya, Huaishuang Shen, Hirohito Hirata, Ning Zhang, Ejun Huang, Masanori Tsubosaka, Qi Gao, Victoria Teissier, Xueping Li, Takeshi Utsunomiya, Stuart B. Goodman
Summary: This study investigated the therapeutic effect of NF-kappa B decoy oligodeoxynucleotides (ODN), mesenchymal stem cells (MSCs), and genetically-modified NF-kappa B sensing interleukin-4 over-secreting MSCs (IL4-MSCs) on chronic inflammation in aged mice. The results demonstrated that MSCs and IL4-MSCs were effective in reducing osteoclast-like cells and increasing the M2/M1 ratio, with IL4-MSCs being more effective in mitigating inflammation. This study suggests potential therapeutic strategies for treating wear particle-associated inflammatory osteolysis after arthroplasty in the elderly.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2023)
Review
Biotechnology & Applied Microbiology
Zhong Alan Li, Shilpa Sant, Sung Kwon Cho, Stuart B. Goodman, Bruce A. Bunnell, Rocky S. Tuan, Michael S. Gold, Hang Lin
Summary: Synovial joint disorders like osteoarthritis and rheumatoid arthritis are common worldwide, but current clinical management does not fully restore joint function. Organ-on-chip (OoC) technology, which can culture multiple human cell-derived tissues/organs, shows potential in developing better therapies for joint diseases.
TRENDS IN BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Meagan J. Makarcyzk, Zhong Alan Li, Ilhan Yu, Haruyo Yagi, Xiurui Zhang, Lauren Yocum, Eileen Li, Madalyn R. Fritch, Qi Gao, Bruce A. Bunnell, Stuart B. Goodman, Rocky S. Tuan, Peter G. Alexander, Hang Lin
Summary: The study established a miniature synovial joint-mimicking microphysiological system called miniJoint, which comprises adipose, fibrous, and osteochondral tissue components derived from human mesenchymal stem cells. This system can be used for studying disease mechanisms and testing drugs, providing an efficient in vitro platform for disease mechanism research and personalized medicine development.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Review
Endocrinology & Metabolism
Luke A. Lopas, Huaishuang Shen, Ning Zhang, Yohan Jang, Vivianne L. Tawfik, Stuart B. Goodman, Roman M. Natoli
Summary: The purpose of this review is to summarize the clinical and basic science methods used to assess fracture healing and propose a framework to improve the translational possibilities. Mainstays of fracture healing assessment include clinical examination, various imaging modalities, and assessment of function. Pre-clinical studies have yielded insight into biomechanical progression as well as the genetic, molecular, and cellular processes of fracture healing. Efforts are emerging to identify early markers to predict impaired healing and possibly early intervention to alter these processes.
CURRENT OSTEOPOROSIS REPORTS
(2023)
Article
Orthopedics
Harsh N. Shah, Andrew A. Barrett, Andrea K. Finlay, Prerna Arora, Michael J. Bellino, Julius A. Bishop, Michael J. Gardner, Matthew D. Miller, James I. Huddleston, William J. Maloney, Stuart B. Goodman, Derek F. Amanatullah
Summary: The aim of this study was to compare the restoration of hip biomechanics between non-arthroplasty surgeons (NAS) and elective arthroplasty surgeons (EAS) through lateral offset, leg length, and acetabular component position. Retrospective review of 131 patients who underwent THA for femoral neck fracture by 7 EAS and 20 NAS was conducted. Results showed that NAS under-restored lateral femoral offset compared to the uninjured side, indicating a risk for under-restoration. However, there was no association found between surgeon type and other biomechanical factors. This study highlights the importance of considering lateral femoral offset in THA for femoral neck fractures, especially when performed by surgeons without regular experience in elective THA.
Article
Orthopedics
Rit Apinyankul, Alexander Y. Hui, Katherine Hwang, Nicole Alexandriadria Segovia, Derek F. Amanatullah, James I. Huddleston, William J. Maloney, Stuart B. Goodman
Summary: Conversion total knee arthroplasty (TKA) with retained periarticular hardware in patients with end-stage knee osteoarthritis can achieve excellent outcomes, but there are unique challenges. The evidence supporting retention versus removal of hardware during TKA is controversial.
JOURNAL OF ARTHROPLASTY
(2023)
Review
Orthopedics
Ian Hollyer, David Ivanov, Shanthi Kappagoda, David W. Lowenberg, Stuart B. Goodman, Derek F. Amanatullah
Summary: Prosthetic joint infection (PJI) after total knee arthroplasty (TKA) is a common and challenging problem. Treatment for PJI depends on patient goals, characteristics, and infection timeline. Two-stage surgery with implant removal, debridement, and antibiotic spacer placement is the most common treatment. Static spacers are used for older, less healthy patients, while mobile spacers are used for younger, healthier patients. Customizable spacers with different antibiotics, cement, and designs are available. Understanding these options is important for optimizing treatment.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Meagan J. Makarczyk, Sophie Hines, Haruyo Yagi, Zhong Alan Li, Alyssa M. Aguglia, Justin Zbikowski, Anne-Marie Padget, Qi Gao, Bruce A. Bunnell, Stuart B. Goodman, Hang Lin
Summary: Osteoarthritis (OA) is a common joint disease that causes pain and disability. The miniJoint model, which mimics the nature of OA in humans, was used to explore the potential of co-treatment with anti-inflammation and chondroinducing agents. The study demonstrated that the combined treatment with BMP-7 and NF-kappa B ODNs reduced inflammation and promoted glycosaminoglycan formation in cartilage, indicating the potential for developing disease-modifying OA drugs.
Review
Immunology
Junichi Kushioka, Simon Kwoon-Ho Chow, Masakazu Toya, Masanori Tsubosaka, Huaishuang Shen, Qi Gao, Xueping Li, Ning Zhang, Stuart B. B. Goodman
Summary: The aging population increases the prevalence of osteoporosis and fragility fractures, impacting patient quality of life and healthcare costs. Chronic inflammation in aging impairs bone regeneration and healing. This review focuses on the bone regeneration process and potential immunomodulatory therapies for improved bone healing in inflammaging.
INFLAMMATION AND REGENERATION
(2023)
Review
Engineering, Biomedical
Emmanuel Gibon, Yuya Takakubo, Stefan Zwingenberger, Jiri Gallo, Michiaki Takagi, Stuart B. Goodman
Summary: The use of biomaterials and implants in orthopedic indications has revolutionized patient care by decreasing pain and improving function. Chronic inflammation and the foreign body response have adverse effects on implant integration. Strategies to mitigate these responses can enhance implant longevity and improve pain relief and overall function.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Article
Materials Science, Biomaterials
Chima Maduka, Oluwatosin M. Habeeb, Maxwell M. Kuhnert, Maxwell Hakun, Stuart B. Goodman, Christopher H. Contag
Summary: Total joint arthroplasties (TJAs) are effective in treating end-stage arthritis, but the challenge is to ensure their long-term viability. Altering immune cell metabolism can reduce inflammation and enhance the integration of artificial joints with bone, preventing loosening.
BIOMATERIALS ADVANCES
(2023)
Article
Immunology
Xueping Li, Huaishuang Shen, Mao Zhang, Victoria Teissier, Ejun Elijah Huang, Qi Gao, Masanori Tsubosaka, Masakazu Toya, Junichi Kushioka, Chima V. Maduka, Christopher H. Contag, Simon Kwoon-Ho Chow, Ning Zhang, Stuart B. Goodman
Summary: This study revealed the dysfunctional bioenergetic activity of bone marrow-derived macrophages (Mφ) and mesenchymal stromal cells (MSCs) exposed to contaminated polyethylene particles (cPE), which may impair the immunoregulatory properties of cells in the bone niche. The underlying molecular defect related to disordered mitochondrial function could represent a potential therapeutic target during the resolution of inflammation.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell Biology
Masakazu Toya, Ning Zhang, Masanori Tsubosaka, Junichi Kushioka, Qi Gao, Xueping Li, Simon Kwoon-Ho Chow, Stuart B. Goodman
Summary: Novel minimally invasive strategies are needed for bone healing in complex fractures and bone defects in the elderly. This study investigated the potential use of CCL2 as a therapeutic target for local cell therapy, as MSCs and macrophages play important roles in bone regeneration and inflammation. Acute stimulation with CCL2 was found to facilitate osteogenesis and macrophage migration in co-culture of MSCs and macrophages.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yuxi Sun, Tatiana Boyko, Owen Marecic, Danielle Struck, Randall K. Mann, Tom W. Andrew, Michael Lopez, Xinming Tong, Stuart B. Goodman, Fan Yang, Michael T. Longaker, Charles K. F. Chan, George P. Yang
Summary: Failure to form bone properly or integrate surgical implants can result in complications and additional interventions in orthopedic surgeries. The role of skeletal stem cells (SSCs) in bone formation is understood, but little is known about the regulation of Bone, Cartilage, Stromal, Progenitors (BCSPs). This study demonstrates that Del1 is a key regulator of BCSP expansion and its knockout leads to reduced bone formation. Del1 promotes BCSP proliferation and prevents apoptosis, making it a potential therapeutic target for improving bone formation and implant success.
