Article
Biochemistry & Molecular Biology
Neha S. Dole, Jihee Yoon, David A. Monteiro, Jason Yang, Courtney M. Mazur, Serra Kaya, Cassandra D. Belair, Tamara Alliston
Summary: This study investigated a microRNA-dependent mechanism mediating the crosstalk between TGF-β and Wnt signaling in osteocytes exposed to fluid shear stress, revealing that miR-100 integrates the functions of these two essential pathways by antagonizing Wnt signaling.
Article
Biotechnology & Applied Microbiology
Mustafa Nile, Matthias Folwaczny, Andrea Wichelhaus, Uwe Baumert, Mila Janjic Rankovic
Summary: This study provides a comprehensive overview and discussion of the methodological technical details regarding fluid flow application in 2D cell culture in vitro experimental conditions. It provides valuable information about cellular molecular events and their quantitative and qualitative analysis, while also confirming the reproducibility of previously published results.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Semra Zuhal Birol, Rana Fucucuoglu, Sertac Cadirci, Ayca Sayi-Yazgan, Levent Trabzon
Summary: This study utilized microfluidic channels with cavitations to mimic the shape of atherosclerotic blood vessels and investigated the changes in inflammatory molecules ICAM-1 and IL-8 in THP-1 cells under different shear stress levels. The results showed that 15 Pa shear stress significantly increased the expression of ICAM-1 gene and IL-8 release in THP-1 cells, while decreasing the adhesion between THP-1 cells and endothelial cells.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Pei-Yu Chu, Han-Yu Hsieh, Pei-Shan Chung, Pai-We Wang, Ming-Chung Wu, Yin-Quan Chen, Jean-Chen Kuo, Yu-Jui Fan
Summary: The objective of this study is to develop a device that mimics the microfluidic system of human arterial blood vessels. The device combines fluid shear stress (FSS) and cyclic stretch (CS) resulting from blood flow and pressure. It enables real-time observation of dynamic morphological changes in cells under different flow fields and stretch. By understanding the effects of FSS and CS on endothelial cells (ECs), including alignment of cytoskeleton proteins with fluid flow and redistribution of paxillin, this study can contribute to the prevention and improvement of cardiovascular diseases.
Article
Biochemistry & Molecular Biology
Bai Zhang, Xueyi Li, Kai Tang, Ying Xin, Guanshuo Hu, Yufan Zheng, Keming Li, Cunyu Zhang, Youhua Tan
Summary: Tumor cells with the ability to adhere to specific organ endothelium exhibit enhanced metastatic tropism to that target organ.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
N. Lara-Castillo, L. Brotto, J. A. Vallejo, K. Javid, M. J. Wacker, L. F. Bonewald, M. L. Johnson
Summary: Skeletal muscle and bone interact mechanically, but also have molecular/biochemical coupling. This study investigated the essential role of muscle and muscle-derived factors in the osteocyte response to mechanical loading. The results suggested that active muscle contraction produces factors necessary for the osteocyte's ability to respond to load. Additionally, specific muscles and myotubes were found to produce factors that alter important signaling pathways involved in the response of osteocytes to mechanical load, indicating a molecular coupling between muscle and bone.
Article
Biochemical Research Methods
Zacchari Ben Meriem, Tiphaine Mateo, Julien Faccini, Celine Denais, Romane Dusfour-Castan, Catherine Guynet, Tatiana Merle, Magali Suzanne, Mickael Di-Luoffo, Julie Guillermet-Guibert, Baptiste Alric, Sylvain Landiech, Laurent Malaquin, Fabien Mesnilgrente, Adrian Laborde, Laurent Mazenq, Remi Courson, Morgan Delarue
Summary: Conventional culture conditions lack dynamic control over the microenvironment, hindering the study of tissues and organisms. In this study, a microfluidic device with sliding elements is presented, allowing for the control of both chemical and mechanical compressive forces. By confining micro-tissues, the impact of growth-induced pressure and macromolecular crowding was studied, shedding light on an understudied type of mechanical stress.
Article
Biochemical Research Methods
Zacchari Ben Meriem, Tiphaine Mateo, Julien Faccini, Celine Denais, Romane Dusfour-Castan, Catherine Guynet, Tatiana Merle, Magali Suzanne, Mickael Di-Luoffo, Julie Guillermet-Guibert, Baptiste Alric, Sylvain Landiech, Laurent Malaquin, Fabien Mesnilgrente, Adrian Laborde, Laurent Mazenq, Remi Courson, Morgan Delarue
Summary: Conventional culture conditions lack dynamic chemical and mechanical control over the microenvironment, making them insufficient for studying tissues, organisms, or 3D multicellular assemblies. This study presents a microfluidic device that allows control of both chemical and mechanical compressive forces, enabling the study of biological samples under specific conditions and the impact of mechanical compression. The device utilizes sliding elements to create reconfigurable closed culture chambers for the study of whole organisms or model micro-tissues.
Article
Immunology
Jennifer Barrila, Jiseon Yang, Karla P. Franco P. Melendez, Shanshan Yang, Kristina Buss, Trenton J. Davis, Bruce J. Aronow, Heather D. Bean, Richard R. Davis, Rebecca J. Forsyth, C. Mark Ott, Sandhya Gangaraju, Bianca Y. Kang, Brian Hanratty, Seth D. Nydam, Eric A. Nauman, Wei Kong, Jason Steel, Cheryl A. Nickerson
Summary: This study investigates the impact of Low Shear Modeled Microgravity (LSMMG) culture on the colonization of Salmonella Typhimurium and human intestinal diseases. The results show that LSMMG culture enhances the colonization ability of Salmonella and that the colonization ability of the Delta hfq mutant strain is higher under LSMMG conditions compared to conventional shaking cultures. In addition, infection by Salmonella induces the expression of genes related to inflammation, tissue remodeling, and wound healing in host cells. This study expands our understanding of how physical forces can affect the early stages of human enteric salmonellosis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Nesrine Bouhrira, Brandon J. DeOre, Peter A. Galie
Summary: This study aims to mimic physiological flow using a low-cost method and investigate the impact of flow separation on velocity and shear stress profiles in a three-dimensional structure. Experimental and computational simulations reveal periodic changes in shear stress and flow separation zones in the flow dynamics.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Chemistry, Multidisciplinary
Xuexia Lin, Jianlong Su, Shufeng Zhou
Summary: The concentration gradient and fluid shear stress in the cell microenvironment were investigated using microfluidic technology. The microfluidic chip was designed using the Darcy-Weisbach equation and computational fluid dynamics modeling, and the distribution of fluid shear stress on the cell model with different micro-channels was studied. The diffusion-convection equation and incompressible laminar flow model were used to simulate the concentration gradient profiles. The experimental data showed agreement with the model simulations, and the method was applied to study single cells, tumor development, and therapeutic applications.
CHINESE CHEMICAL LETTERS
(2022)
Article
Cell Biology
Alper D. Ozkan, Tina Gettas, Audrey Sogata, Wynn Phaychanpheng, Miou Zhou, Jerome J. Lacroix
Summary: The study developed a genetically encoded fluorescent reporter, iGlow, by inserting a fluorescent protein into the third intracellular loop of GPR68, which responds to physiological GPR68 activators and synthetic agonists with specificity. iGlow activation is unaffected by pharmacological modulation of downstream G-protein signaling or disruption of actin filaments, providing a new tool for investigating GPR68-dependent signaling in health and disease.
JOURNAL OF CELL SCIENCE
(2021)
Article
Biochemical Research Methods
Hao Yang, Tao Chen, Yichong Hu, Fuzhou Niu, Xinyu Zheng, Haizhen Sun, Liang Cheng, Lining Sun
Summary: The function of vascular endothelial cells (ECs) is regulated by biochemical cues, cell-cell interactions, and fluid shear stress. A multi-layer microfluidic chip was developed to study the effects of flow rate and TNF-alpha on the mechanical properties of ECs. The results showed the importance of hemodynamics and the adverse impact of inflammation on the vascular endothelium.
Article
Biochemistry & Molecular Biology
Anushree Dwivedi, Patrick A. Kiely, David A. Hoey
Summary: Bone is a common site for breast cancer metastasis, with mechanically stimulated osteocytes releasing factors that enhance cancer cell proliferation and migration. The secretome of mechanically activated osteocytes contains specific cytokines that potentially drive breast cancer metastasis to bone.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Niaz Oliazadeh, Kristen F. Gorman, Mohamed Elbakry, Alain Moreau
Summary: This study characterizes the mechanosensory defect in adolescent idiopathic scoliosis (AIS) osteoblasts by examining cilia length dynamics, actin filament rearrangement, cell migration, and secretion of VEGF-A and PGE2. The findings suggest that there are abnormalities in cellular mechanotransduction in AIS, in addition to observed primary cilium defects. This altered mechanotransduction may play a role in the pathomechanism of AIS and should be considered in the development of more effective bracing treatments.
SCIENTIFIC REPORTS
(2022)
Article
Materials Science, Biomaterials
Shashi Malladi, David Miranda-Nieves, Lian Leng, Stephanie J. Grainger, Constantine Tarabanis, Alexander P. Nesmith, Revanth Kosaraju, Carolyn A. Haller, Kevin Kit Parker, Elliot L. Chaikof, Axel Guenther
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2020)
Article
Multidisciplinary Sciences
Onaizah Onaizah, Liangcheng Xu, Kevin Middleton, Lidan You, Eric Diller
Article
Endocrinology & Metabolism
Shubo Wang, Shaopeng Pei, Murtaza Wasi, Ashutosh Parajuli, Albert Yee, Lidan You, Liyun Wang
Summary: Moderate mechanical loading can suppress tumor-induced bone destruction in breast cancer, but higher magnitude loading can accelerate bone destruction. Different exercise regimens have varying effects on bone cells and bone marrow. Optimization of exercise parameters is needed to harness the skeletal benefits of exercise in metastatic breast cancer.
Article
Materials Science, Biomaterials
Zhe Zheng, Jianchao Qi, Liqiu Hu, Dongfang Ouyang, Huizhen Wang, Qili Sun, Lijun Lin, Lidan You, Bin Tang
Summary: In this study, a hydrogel dressing containing cannabidiol was designed and fabricated. The hydrogel exhibited good biocompatibility, antibacterial activity, and angiogenesis properties. It also showed the ability to scavenge free radicals and reduce inflammatory responses. In vivo studies demonstrated that the hydrogel significantly facilitated the wound healing process.
BIOMATERIALS ADVANCES
(2022)
Article
Oncology
Chun-Yu Lin, Xin Song, Yaji Ke, Arjun Raha, Yuning Wu, Murtaza Wasi, Liyun Wang, Fei Geng, Lidan You
Summary: The combined treatment of Yoda1 and low-magnitude high-frequency vibration can effectively reduce bone loss caused by bone metastasis, inhibit osteoclast formation, and suppress breast cancer cell migration.
Review
Endocrinology & Metabolism
Chun-Yu Lin, Xin Song, Kimberly Seaman, Lidan You
Summary: This review summarizes the recent microfluidic studies on mechanically stimulated osteocytes in regulating other cell types. Microfluidic platforms allow researchers to mimic multicellular environments and study the regulation of osteocytes under mechanical stimulation. These studies contribute to the development of treatment approaches targeting osteocytes under mechanical stimulation.
CURRENT OSTEOPOROSIS REPORTS
(2022)
Article
Multidisciplinary Sciences
Xin Song, Chun-Yu Lin, Xueting Mei, Liyun Wang, Lidan You
Summary: LMHF vibration can inhibit cancer extravasation in bones by activating osteocytes, suggesting its potential to suppress bone metastasis in breast cancer.
Review
Engineering, Mechanical
Xin Song, Amel Sassi, Kimberly Seaman, Chun-Yu Lin, Lidan You
Summary: Patients with cancer and metastatic bone lesions often experience skeletal-related events (SREs) that greatly impact their quality of life. Whole body vibration (WBV) has shown promising results in improving bone health and reducing cancer progression. WBV not only improves bone mineral density and volume, but also reduces tumor burden and promotes interactions between bone cells and cancer cells to inhibit metastasis. WBV could potentially serve as a new intervention or adjuvant treatment for cancer patients.
Article
Nanoscience & Nanotechnology
Dongfang Ouyang, Hanqing Zhang, Bin Tang, Jaewon Park, Lina Hu, Jenny Hirst, Lidan You, Yonghua Li
Summary: Circulating clonal plasma cells have emerged as an important biomarker for evaluating minimal residual disease in multiple myeloma. Microfluidic techniques have been successfully used to develop a specific chip for detecting these cells, showing promising clinical utility with good agreement to existing methods.
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2022)
Meeting Abstract
Endocrinology & Metabolism
Chun-Yu Lin, Xin Song, Yaji Ke, Liyun Wang, Lidan You
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Meeting Abstract
Endocrinology & Metabolism
Xin Song, Chun-Yu Lin, Arjun Raha, Yaji Ke, Liyun Wang, Fei Geng, Lidan You
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Meeting Abstract
Endocrinology & Metabolism
Murtaza Wasi, Shubo Wang, Jinhu Xiong, Fei Geng, Lidan You, Liyun Wang
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Article
Multidisciplinary Sciences
Wei Chen, Shihyun Park, Chrishma Patel, Yuxin Bai, Karim Henary, Arjun Raha, Saeed Mohammadi, Lidan You, Fei Geng
Summary: Matrix stiffness influences the proliferation and migration ability of breast cancer cells, with YAP playing a significant role in this process. As matrix stiffness increases, highly metastatic breast cancer cells exhibit a different migratory potential compared to non-metastatic cells, and this process is YAP-dependent.
Article
Chemistry, Multidisciplinary
Renee M. Ripken, Jeffery A. Wood, Stefan Schlautmann, Axel Gunther, Han J. G. E. Gardeniers, Severine Le Gac
Summary: Guiding bubble nucleation in a microfluidic device can optimize the performance of multiphase catalytic microreactors. Experimental results show that the presence of bubbles has a significant impact on transport phenomena and reaction conversion within the reactor.
REACTION CHEMISTRY & ENGINEERING
(2021)
Article
Cell Biology
Liangcheng Xu, Xin Song, Gwennyth Carroll, Lidan You
Summary: Osteocytes are the major mechanosensing cells in bone remodeling, and this project aims to design and fabricate a multi-shear stress, co-culture platform to study the interaction between osteocytes and other bone cells under varying flow conditions. By utilizing microfluidic devices, significant differences in RANKL levels between channels with different shear stress levels were observed, showing that pre-osteoclast differentiation is directly affected by adjacent flow-stimulated osteocytes. The platform provides an optimal tool for bone cell mechanistic studies and potential drug target discovery for clinical treatments of bone-related diseases.
INTEGRATIVE BIOLOGY
(2020)
Article
Biophysics
Nathan D. Camarillo, Rafael Jimenez-Silva, Frances T. Sheehan
Summary: This article discusses the statistical dependence between multiple measurements from the same participant and provides recommendations for using these measurements when they are not independent.
JOURNAL OF BIOMECHANICS
(2024)
Article
Biophysics
J. Huet, A. -S. Boureau, A. Sarcher, C. Cornu, A. Nordez
Summary: Standard compression in freehand 3D ultrasound induces a bias in volume calculations, but minimal compression and gel pad methods have similar results. With a trained examiner and precautions, the bias can be minimized and become acceptable in clinical applications.
JOURNAL OF BIOMECHANICS
(2024)
Article
Biophysics
C. Lariviere, A. H. Eskandari, H. Mecheri, F. Ghezelbash, D. Gagnon, A. Shirazi-Adl
Summary: Recent developments in musculoskeletal modeling have focused on model customization. Personalization of the spine profile may affect estimates of spinal loading and stability. This study investigates the biomechanical consequences of changes in the spinal profile and finds that personalizing the spine profile has medium to large effects on trunk muscle forces and negligible to small effects on spinal loading and stability.
JOURNAL OF BIOMECHANICS
(2024)
Article
Biophysics
Luke T. Mattar, Arash B. Mahboobin, Adam J. Popchak, William J. Anderst, Volker Musahl, James J. Irrgang, Richard E. Debski
Summary: Exercise therapy fails in about 25.0% of cases for individuals with rotator cuff tears, and one reason for this failure may be the inability to strengthen and balance the muscle forces that keep the humeral head in the correct position. This study developed computational musculoskeletal models to compare the net muscle force before and after exercise therapy between successfully and unsuccessfully treated patients. The study found that unsuccessfully treated patients had less inferiorly oriented net muscle forces, which may increase the risk of impingement.
JOURNAL OF BIOMECHANICS
(2024)
Article
Biophysics
Natsuki Sado, Takeshi Edagawa, Toshihide Fujimori, Shogo Hashimoto, Yoshikazu Okamoto, Takahito Nakajima
Summary: The existing methods for predicting hip and lumbosacral joint centres in Japanese adults are biased and differ between sexes. We propose new regression equations that consider soft-tissue thickness, sex differences, and a height-directional measure, and validate them using leave-one-out cross-validation.
JOURNAL OF BIOMECHANICS
(2024)
Article
Biophysics
Peimin Yu, Xuanzhen Cen, Qichang Mei, Alan Wang, Yaodong Gu, Justin Fernandez
Summary: This study aimed to explore the intra-foot biomechanical differences among individuals with chronic ankle instability (CAI), copers, and healthy individuals during dynamic tasks. The study found that copers and CAI individuals had smaller dorsiflexion angles and copers presented a more eversion position compared to healthy participants. Copers also had greater dorsiflexion angles in the metatarsophalangeal joint and more inversion moments in the subtalar joint during certain tasks. These findings can help in designing interventions to restore ankle joint functions in CAI individuals.
JOURNAL OF BIOMECHANICS
(2024)
Article
Biophysics
Jon Skovgaard Jensen, Anders Holsgaard-Larsen, Anders Stengaard Sorensen, Per Aagaard, Jens Bojsen-Moller
Summary: This study investigates the biomechanical effects of robot-assisted body weight unloading (BWU) on gait patterns in healthy young adults. The results show that dynamic robot-assisted BWU enables reduced kinetic requirements without distorting biomechanically normal gait patterns during overground walking.
JOURNAL OF BIOMECHANICS
(2024)