Article
Biochemistry & Molecular Biology
M. C. Linju, M. R. Rekha
Summary: The present study developed a L-proline conjugated chitosan scaffold for wound healing. The scaffold was characterized by various tests and showed no cytotoxicity. In vitro experiments demonstrated that the modified scaffold promoted wound closure and collagen deposition. These findings suggest that the L-proline conjugated scaffold has potential as a wound dressing to improve wound healing.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Joanna Wisniewska, Magda Slyszewska, Karolina Stalanowska, Katarzyna Walendzik, Marta Kopcewicz, Sylwia Machcinska, Barbara Gawronska-Kozak
Summary: The study found that exposure of pASCs to hypoxia increased levels of VEGF in the conditioned media, which promoted the migratory ability of skin cells and affected the expression of markers related to wound healing. Hypoxia-conditioned media enhanced skin cell functions, highlighting the potential of using ASCs' paracrine activity to modulate wound healing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Yunchuan Wang, Yue Zhang, Ting Li, Kuo Shen, Ke Jia Wang, Chenyang Tian, Dahai Hu
Summary: Engineered skin substitutes derived from human skin reduce inflammatory reactions and are easier to use in clinics. Type I collagen and platelet-rich plasma are mixed to form a stable scaffold, while adipose mesenchymal stem cell derived exosomes improve the performance of the engineered skin. This method provides a new therapeutic strategy and theoretical basis for tissue regeneration and wound repair.
ADVANCED MATERIALS
(2023)
Article
Cell & Tissue Engineering
Yang Zhou, Bo Zhao, Xin-Liao Zhang, Yi-jun Lu, Shou-Tao Lu, Jian Cheng, Yu Fu, Lin Lin, Ning-Yan Zhang, Pei-Xin Li, Jing Zhang, Jun Zhang
Summary: This study investigated the potential clinical application roles of human adipose-derived mesenchymal stem cells (hADSC) and hADSC-derived exosomes (hADSC-Exo) in cutaneous wound healing. The combined administration of local hADSC-Exo smearing and hADSC/hADSC-Exo intravenous administration showed additional benefits in promoting wound healing, accelerating re-epithelialization, reducing scar widths, and enhancing angiogenesis and collagen synthesis.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Dermatology
Tian Yuan, Li Meijia, Chen Xinyao, Chen Xinyue, Hao Lijun
Summary: Exosomes derived from human adipose-derived stem cells (ADSCs) have shown excellent capability in improving wound healing quality, but their efficacy in clinical applications is still uncertain. This study conducted a systematic review of preclinical studies comparing exosomes from human ADSCs with placebo in animal models of wound closure. The results showed that administration of exosomes derived from human ADSCs significantly improved wound closure compared to controls, with the same effect as ADSCs. The therapeutic effect was further enhanced by modified ADSC-EV. This suggests that exosomes derived from human ADSCs, especially after enrichment for specific non-coding RNA, have promising potential to improve healing efficiency.
INTERNATIONAL WOUND JOURNAL
(2023)
Article
Pharmacology & Pharmacy
Hsiang-Hao Hsu, Aline Yen Ling Wang, Charles Yuen Yung Loh, Ashwin Alke Pai, Huang-Kai Kao
Summary: This study found that diabetic ASC-Exo enhance cutaneous wound healing by stimulating dermal cell proliferation, keratinocyte proliferation, and angiogenesis. The diabetic ASC-Exo also stimulate resident monocytes/macrophages to secrete more TGF-beta 1 and activate the TGF-beta/Smad3 signaling pathway.
Article
Nanoscience & Nanotechnology
Honghao Yu, Yiping Wu, Boyu Zhang, Mingchen Xiong, Yi Yi, Qi Zhang, Min Wu
Summary: This study demonstrated that E2F1-deficient ADSC-Exos promote wound healing by enhancing collagen formation and angiogenesis, and by regulating miRNA to enhance fibroblast activities. This finding provides a novel strategy of gene-engineered stem cell exosomes for accelerating wound healing.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Engineering, Biomedical
Nan Hu, Zhengwei Cai, Xudong Jiang, Chuang Wang, Tao Tang, Tianze Xu, Hong Chen, Xiaoqiang Li, Xiaolong Du, Wenguo Cui
Summary: Adipose-derived stem cell (ADSC)-originating exosomes (ADSC-Exos) have potential in diabetic wound repair, but their disadvantages limit their clinical application. This study developed hypoxia-pretreated ADSC-Exo (ADSC-HExo)-embedded GelMA hydrogels (GelMA-HExo) and demonstrated their therapeutic effects in promoting diabetic wound healing. These findings suggest a new therapeutic strategy for diabetic ulcers.
ACTA BIOMATERIALIA
(2023)
Review
Cell Biology
Yang An, Shuyan Lin, Xiaojie Tan, Shiou Zhu, Fangfei Nie, Yonghuan Zhen, Luosha Gu, Chunlei Zhang, Baicheng Wang, Wei Wei, Dong Li, Junhao Wu
Summary: ADSCs-EXOs modulate immune responses and inflammation, promote angiogenesis, accelerate proliferation and re-epithelization of skin cells, and regulate collagen remodelling to inhibit scar hyperplasia in wounds. Compared with ADSCs therapeutics, ADSCs-EXOs have high stability, easy storage, immune system non-rejection, homing effect, and easily controlled dosage. In patients with diabetes mellitus, ADSCs-EXOs can improve fat grafting and promote wound healing.
CELL PROLIFERATION
(2021)
Review
Immunology
Qingyi Jia, Hanxing Zhao, Yixi Wang, Ying Cen, Zhenyu Zhang
Summary: Wound healing is a complex process that involves multiple phases, including inflammation, proliferation, and remodeling. Abnormal inflammation can impede wound healing under certain pathological conditions. Adipose-derived stem cells (ADSCs) and their secreted extracellular vesicles (ADSC-EVs) have shown potential in promoting wound healing by inhibiting abnormal inflammation responses.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Physiology
Peng Zhou, Vanessa Chrepa, Ioannis Karoussis, Michael A. Pikos, Georgios A. Kotsakis
Summary: This study found that an herbal extract compound-based oral rinse was cytocompatible to cells critical to oral wound healing and promoted fibroblast migration and differentiation, contrary to existing antiseptic rinses that lack selective cytotoxicity.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Cell Biology
Mingming Wang, Hongyan Zhan, Jianhua Wang, Hua Song, Jianhua Sun, Gang Zhao
Summary: This study demonstrates that CS can enhance the proliferation of ADSCs and improve their migration ability through inhibiting apoptosis and oxidative stress. CS also promotes the expression of CXCR4 in ADSCs and enhances their migration and angiogenic capacity. Importantly, CS improves the efficacy of ADSCs in wound healing both in vivo and in vitro, providing potential materials for the treatment of wound healing.
Article
Cell & Tissue Engineering
Stefan Balko, Evan Kerr, Ed Buchel, Sarvesh Logsetti, Afshin Raouf
Summary: Stromal vascular fraction (SVF) cells and keratinocytes engage in a paracrine crosstalk during wound closure, resulting in a new cytokine profile that can be used to accelerate wound healing and initiate re-epithelialization.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Cell & Tissue Engineering
Yang Zhou, Xing-Liao Zhang, Shou-Tao Lu, Ning-Yan Zhang, Hai-Jun Zhang, Jing Zhang, Jun Zhang
Summary: This study explores the potential clinical application of hADSCs-Exos encapsulated in PF-127 hydrogel for wound healing. The results showed that PF-127/hADSCs-Exos complexes enhanced skin wound healing, promoted re-epithelialization, increased collagen synthesis and expression of skin barrier proteins, and reduced inflammation. Using PF-127/hADSCs-Exos complexes allows for lower doses and frequency of exosome administration while maintaining therapeutic effects.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell Biology
Jiachao Xiong, Hong Qiang, Ting Li, Jiayi Zhao, Ziyu Wang, Fei Li, Jianwen Xu
Summary: The study demonstrates that human adipose-derived stem cells can promote the vascularization of seawater-treated wounds by differentiating into vascular endothelial cells and promoting their proliferation and migration. Bioinformatics analysis identified genes that may regulate wound healing mechanisms such as extracellular matrix remodeling, programmed cell death, inflammation, and vascularization.
Article
Cell Biology
Qiuyue Peng, Guoqiang Ren, Zongzhe Xuan, Martyna Duda, Cristian Pablo Pennisi, Simone Riis Porsborg, Trine Fink, Vladimir Zachar
Summary: This study investigated the evolution of different surface marker profiles in in vitro expanded adipose-derived stem cells (ASCs) and found that specific subpopulations exhibited better growth and therapeutic capabilities. These surface epitopes may serve as tools to enhance specific functionality and improve therapeutic outcomes.
Review
Biotechnology & Applied Microbiology
Behnaz Bakhshandeh, Nika Ranjbar, Ardeshir Abbasi, Elahe Amiri, Ali Abedi, Mohammad-Reza Mehrabi, Zahra Dehghani, Cristian Pablo Pennisi
Summary: Tissue engineering is a cutting-edge discipline that combines cells, scaffolds, and induction factors to regenerate and repair damaged tissue. Understanding the effects of biochemical and biophysical factors on cell fate is crucial for improving the efficiency of tissue engineering approaches. Biophysical signals provide a robust set of stimuli to manipulate cell signaling pathways during tissue formation.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Cell & Tissue Engineering
Zongzhe Xuan, Qiuyue Peng, Thomas Larsen, Leonid Gurevich, Jesper de Claville Christiansen, Vladimir Zachar, Cristian Pablo Pennisi
Summary: In this study, gelatin methacrylate (GelMA)/alginate hydrogels were optimized for bioprinting three-dimensional (3D) smooth muscle cell (SMC) constructs. The group with the highest concentrations of GelMA and alginate exhibited the highest viscosity and stability, supporting high cell viability, proliferation, and differentiation towards a contractile SMC phenotype. The results suggest that adjusting the composition of GelMA/alginate hydrogels is an effective means of controlling the SMC phenotype and provides a promising approach for studying smooth muscle adaptation and therapies for urethral strictures.
TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2023)
Article
Dentistry, Oral Surgery & Medicine
Thomas Starch-Jensen, Rubens Spin-Neto, Pernille Veiss-Pedersen, Christer Dahlin, Niels Henrik Bruun, Trine Fink
Summary: This study aimed to test the hypothesis that there would be no difference in radiographic outcome between maxillary sinus floor augmentation (MSFA) with allogeneic adipose tissue-derived stem cells (ASCs) seeded on depro-teinized bovine bone mineral (DBBM) and excipient on DBBM. The results showed that the GV was larger in the test group at one month but larger in the control group at four months. BD increased with both treatments, and the control group had higher BD at four months. The study concluded that allogeneic ASCs seeded on DBBM with MSFA did not improve the radiographic outcome compared with DBBM alone.
JOURNAL OF CRANIO-MAXILLOFACIAL SURGERY
(2023)
Correction
Cell Biology
Qiuyue Peng, Guoqiang Ren, Zongzhe Xuan, Martyna Duda, Cristian Pablo Pennisi, Simone Riis Porsborg, Trine Fink, Vladimir Zachar
Review
Materials Science, Multidisciplinary
N. Pien, H. Krzyslak, S. Shastry Kallaje, J. Van Meerssche, D. Mantovani, C. De Schauwer, P. Dubruel, S. Van Vlierberghe, C. P. Pennisi
Summary: Additive manufacturing technologies have revolutionized tissue engineering by enabling the fabrication of complex tissues with intricate geometries. This review discusses recent advances in tissue engineering fabrication methods, with a focus on hierarchically organized anisotropic tissues. The importance of considering anatomical structure and function in the design process is emphasized, along with the factors influencing fabrication strategy and the advantages and limitations of different methods.
APPLIED MATERIALS TODAY
(2023)
Article
Biotechnology & Applied Microbiology
Simone Riis Porsborg, Hubert Krzyslak, Malgorzata K. Pierchala, Vincent Trole, Konstantin Astafiev, Rasmus Lou-Moeller, Cristian Pablo Pennisi
Summary: Excessive skin scarring affects a large number of patients worldwide, and an effective treatment has yet to be found. This study demonstrates the potential of ultrasound for scar reduction by developing a device based on printable piezoelectric material. The device was used to treat human fibroblasts with ultrasound, resulting in a significant reduction in fibroblast growth and extracellular matrix deposition. The overall results suggest that ultrasound treatment could be a beneficial therapy for scar reduction.
BIOENGINEERING-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Zongzhe Xuan, Leonid Gurevich, Jesper de Claville Christiansen, Vladimir Zachar, Cristian Pablo Pennisi
Summary: In this study, the effects of cyclic mechanical stimulation on smooth muscle cell maturation were investigated using three-dimensional constructs. The results showed that methacrylate-modified surfaces supported stable adhesion of the 3D constructs and facilitated cyclic mechanical stimulation, resulting in increased expression of contractile markers. These effects were mediated by activation of the p38 MAPK pathway. These findings provide valuable insights into the role of mechanical signaling in maintaining the contractile phenotype of bladder smooth muscle cells and have important implications for future treatments of LUT diseases.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Review
Oncology
Behnaz Bakhshandeh, Shokufeh Ghasemian Sorboni, Nika Ranjbar, Roham Deyhimfar, Maryam Sadat Abtahi, Mehrnaz Izady, Navid Kazemi, Atefeh Noori, Cristian Pablo Pennisi
Summary: Stem cells are influenced by biochemical and biophysical cues from the extracellular matrix and neighboring cells. Biomechanical forces play a crucial role in regulating stem cell behavior and fate through mechanotransduction. Recent studies have highlighted the importance of biomechanical signals in tissue regeneration and shown the existence of precise control mechanisms in stem cell niches.
EXPERIMENTAL CELL RESEARCH
(2023)