Article
Chemistry, Applied
Aline Pereira de Oliveira, Juliana Naozuka, Julio Alberto Landero-Figueroa
Summary: This study investigates the potential of Pleurotus ostreatus and Pleurotus djamor mushrooms to absorb and accumulate selenium in organic substrate. The results show that Pleurotus djamor grown on Se(IV)-supplemented substrate has the greatest potential for selenium uptake and accumulation. Various selenium species, including selenomethionine, selenocystine, and Se-methylselenocysteine, were identified in the mushrooms. In vitro digestion assays showed high bioaccessibility of selenium. The bioaccessible selenium species were found to be mainly associated with low molecular weight proteins in the mushrooms.
Review
Chemistry, Applied
Kangjia Sheng, Cuiling Wang, Bitao Chen, Meijuan Kang, Minchang Wang, Ke Liu, Ming Wang
Summary: Lentinus edodes, commonly known as shiitake mushroom, is a widely cultivated edible mushroom in East Asia with rich bioactive polysaccharides, especially beta-glucans. Research on the polysaccharides from L. edodes has shown various remarkable biological activities such as antioxidant, anti-tumor, and immunomodulatory effects, drawing attention from scholars worldwide. Recent studies on the isolation methods, structural features, bioactivities, and mechanisms of L. edodes polysaccharides provide valuable research insights for their further application as therapeutic agents and functional foods.
Article
Biochemistry & Molecular Biology
Xinling Song, Wenxin Cai, Zhenzhen Ren, Le Jia, Jianjun Zhang
Summary: The study shows that acidic-hydrolysis Lentinus edodes residue polysaccharides (Ac-LRP) have antioxidant and hepatoprotective effects on lipopolysaccharide-induced liver injury in mice, reducing the incidence of liver necrosis and potentially serving as a treatment for liver injury.
INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS
(2021)
Article
Horticulture
Xuemei Zhao, Yudi Wang, Zhiyong Zhang, Libin Sun, Yunyun Wei, Xiujing Bao, Guang Xin
Summary: The study found that short-time partial dehydration (STPD) treatment, especially STPD-2, can effectively preserve the quality of shiitake mushrooms, including maintaining firmness, delaying browning, and preserving umami components.
SCIENTIA HORTICULTURAE
(2021)
Article
Biochemistry & Molecular Biology
Chidube Anthony Alagbaoso, Masashi Mizuno
Summary: The polysaccharides from Lentinus edodes suppressed colitis in mice and inhibited necroptotic cell death in Caco-2 cells in a dose-dependent manner. The polysaccharide extract exerted a remarkable inhibitory effect on the receptor-interacting protein kinase RIPK1-RIPK3-MLKL necroptosis signaling cascade in the colon of mice with colitis. The anti-inflammatory and antinecroptotic activities of the polysaccharide sample were found to be dependent on the carbohydrate-rich fraction of the polysaccharides.
INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS
(2021)
Article
Food Science & Technology
Rongrong Xia, Xuemei Zhao, Guang Xin, Libin Sun, Heran Xu, Zhenshan Hou, Yunting Li, Yafei Wang
Summary: This study investigates the molecular mechanisms of energy status related to the umami taste of postharvest shiitake mushrooms during spore release. The results suggest that energy levels are strongly correlated with umami taste, and downregulation of related gene expression in the energy metabolism pathway was observed.
FOOD SCIENCE AND HUMAN WELLNESS
(2023)
Article
Chemistry, Applied
Aleksandra Sknepnek, Sergej Tomic, Dunja Miletic, Steva Levic, Miodrag Colic, Viktor Nedovic, Miomir Niksic
Summary: The study investigated the preparation of a novel kombucha beverage using medicinal mushrooms, with Coriolus versicolor extract showing more complex polysaccharides and higher contents of total polysaccharides, phenols, and flavonoids compared to Lentinus edodes extract. The extracts were not cytotoxic for PBMC in vitro, but exhibited immunomodulatory effects dependent on their chemical compositions, particularly in reducing Th2 cytokines and IL-10 in PBMC cultures. Novel kombucha products could be recommended as functional beverages or nutraceuticals with potentially beneficial immunomodulatory effects in allergies.
Review
Plant Sciences
Jiangtao Hu, Zheng Wang, Li Zhang, Jie Peng, Tao Huang, Xiao Yang, Byoung Ryong Jeong, Qichang Yang
Summary: Seleno-amino acids are safe and beneficial compounds for human health, and research has focused on their forms and metabolism in vegetables. To ensure safe intake of selenium, it is important to address the issues of improving seleno-amino acid accumulation and controlling total selenium and seleno-amino acid contents in vegetables. The use of plant factories with artificial lighting and various analytical technologies may help resolve these issues. Additionally, a proposed production system could allow for the precise control of selenium content in vegetables, producing specified amounts of selenium and high proportions of seleno-amino acids.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Yiwen Zhang, Yanfei Cui, Yanbo Feng, Fengping Jiao, Le Jia
Summary: Acute lung injury (ALI) is a lung disease characterized by acute dyspnea, pulmonary inflammation, respiratory distress, and non-cardiogenic pulmonary edema, accompanied by the mid- and end-stage characteristics of COVID-19. Lentinus edodes polysaccharides (PLE) have the potential to alleviate ALI by inhibiting oxidative stress and inflammation and enhancing antioxidant enzyme activity in the lung.
Article
Food Science & Technology
Fei Xia, Shiling Cao, Mengwen Wang, Yujiao Sun
Summary: This study comprehensively evaluated the extraction, structural characterization, and hypoglycemic activity of L. edodes polysaccharides. Optimal extraction conditions were determined, and the polysaccharide component LNT was found to have a three-stranded helical structure with a molecular weight of 3.95 x 105 Da and a composition of glucose, galactose, mannose, and arabinose. LNT exhibited significant in vitro hypoglycemic activity and improved insulin resistance by regulating the expression of PI3K and P-AKT proteins in the PI3K/AKT signaling pathway.
Article
Chemistry, Medicinal
Wei Hou, Hewei Dong, Ying Yao, Kangyin Pan, Guang Yang, Peixiang Ma, Hongtao Xu
Summary: This research proposes the development of clickable selenylation reactions for the synthesis of Se-containing compounds, aiming to boost the development of seleno-medicinal chemistry.
Article
Biochemistry & Molecular Biology
Yuxi Guo, Xuefeng Chen, Pin Gong, Jing Guo, Dan Deng, Guanglian He, Chenglong Ji, Ruotong Wang, Hui Long, Jiating Wang, Wenbo Yao, Wenjuan Yang, Fuxin Chen
Summary: Treatment with polysaccharides isolated from Lentinula edodes stalks significantly improved the qualities of fresh mushrooms during storage, such as reduced weight loss, retention of hardness and springiness, and improved soluble protein content. The polysaccharides also enhanced the antioxidant activities and inhibited browning-related enzymes to prolong the shelf life.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Applied
Jiapei Xi, Xiao Chen, Jiaxin Du, Lei Zhong, Qiuhui Hu, Liyan Zhao
Summary: This study investigated the biosynthesis and changes of organic sulfide in shiitake mushrooms during hot-air drying. The study monitored aw, moisture migration, taste and flavor profiles, organic sulfide content, and enzyme activities during three drying procedures (CT/ST1/ST2). The results showed that drying rate was influenced by moisture migration. Key enzymes involved in the process were heat-resistant proteases, except for C-Dase which had low thermal stability. A total of 17 organic sulfides were identified, with 6 cyclic polysulfides formed by C-Dase desulfurization and 5 thioethers generated through thermal cleavage and Maillard reaction. ST2 drying procedures positively influenced the formation of cyclic polysulfides and the development of premium flavor qualities.
Article
Biochemistry & Molecular Biology
Yuxi Guo, Xuefeng Chen, Pin Gong, Hui Long, Jiating Wang, Zhenfang Deng, Ruotong Wang, Aoyang Han, Zhuoya Qi, Wenbo Yao, Wenjuan Yang, Jing Wang, Nan Li, Fuxin Chen
Summary: The aim of this study was to prepare a film based on shiitake (Lentinus edodes) stalk polysaccharides (LEP) for mushroom preservation. The effects of different LEP concentrations on physical, mechanical, antioxidant, and antimicrobial properties of the prepared film were evaluated. The addition of 1.5 % LEP resulted in homogeneous distribution in the prepared film, as well as greatly improved its antimicrobial properties. Moreover, LEP film resulted in superior mushroom preservation by regulating enzyme activities related to mushroom browning and softening, thereby decaying these processes. The prepared film maintained mushroom quality by reducing the accumulation of H2O2 and activating the regulatory system against oxidative stress. The findings highlight the potential benefits of LEP films as a strategy to improve mushroom quality and prevent post-harvest spoilage.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Oncology
Yuanyuan Wang, Yanqiong Zeng, Linyu Zhu, Jiajia Wan, Ningjing Lei, Xiaohan Yao, Xixi Duan, Yana Zhang, Yanru Cheng, Ning Tao, Zhihai Qin
Summary: Polysaccharides from Lentinus edodes have been shown to inhibit lymphatic metastasis of colorectal cancer by reducing the secretion of VEGF-C by cancer associated fibroblasts. This effect is mediated through the TLR4/JNK signaling pathway, suggesting a potential new strategy for the comprehensive treatment of CRC.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Marzenna Klimaszewska, Sabina Gorska, Grzegorz Lapienis, Beata Kaleta, Sandra Gorska, Marta Kaszowska, Maciej Dawidowski, Andrzej Gamian, Radoslaw Zagozdzon, Andrzej Gorski, Jadwiga Turlo
Summary: This study investigated the immunosuppressive activity of a selenium-containing polysaccharide fraction isolated from Lentinula edodes, identifying four polysaccharide components with varying levels of biological activity. While all components displayed inhibitory effects on lymphocyte proliferation, the isolated polysaccharides showed weaker but more selective activity compared to the crude fraction.
Article
Cell Biology
Agata Barzowska, Barbara Pucelik, Katarzyna Pustelny, Alex Matsuda, Alicja Martyniak, Jacek Stepniewski, Anna Maksymiuk, Maciej Dawidowski, Ulli Rothweiler, Jozef Dulak, Grzegorz Dubin, Anna Czarna
Summary: This study demonstrates the efficacy of a set of small molecule inhibitors of DYRK1A kinase in promoting beta-cell proliferation, enhancing long-term insulin secretion, and balancing glucagon level in human islets. The compounds show significantly more pronounced effects compared to harmine and hold considerable promise for regenerative medicine in the treatment of T1DM and T2DM.
Article
Physiology
Piotr Konopelski, Dawid Chabowski, Marta Aleksandrowicz, Ewa Kozniewska, Piotr Podsadni, Agnieszka Szczepanska, Marcin Ufnal
Summary: Recent evidence suggests that the gut bacteria-derived metabolite indole-3-propionic acid (IPA) may increase blood pressure in rats through cardiac and vascular mechanisms. Additionally, IPA could enhance cardiac contractility and metabolic activity of cardiomyocytes.
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
(2021)
Article
Chemistry, Medicinal
Roberto Fino, Dominik Lenhart, Vishal C. Kalel, Charlotte A. Softley, Valeria Napolitano, Ryan Byrne, Wolfgang Schliebs, Maciej Dawidowski, Ralf Erdmann, Michael Sattler, Gisbert Schneider, Oliver Plettenburg, Grzegorz M. Popowicz
Summary: This study aims to discover and develop new potential compounds to treat trypanosomiasis by targeting the PEX14-PEX5 interaction through the synergy between structural biology, computer-aided drug design, and medicinal chemistry.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2021)
Article
Biochemistry & Molecular Biology
Beata Kaleta, Aleksander Roszczyk, Michal Zych, Monika Kniotek, Radoslaw Zagozdzon, Marzenna Klimaszewska, Eliza Malinowska, Michal Pac, Jadwiga Turlo
Summary: The study revealed the significant immunosuppressive activity of the Lentinula edodes-derived Se-Le-30 fraction on human immune cells, showing inhibition of proliferation in PBMCs, down-regulation of inflammatory cytokine production in T cells, and reduction in cytotoxicity of NK cells.
Article
Environmental Sciences
Agata Sosnicka, Bartosz Kozka, Katerina Makarova, Joanna Giebultowicz, Marzenna Klimaszewska, Jadwiga Turlo
Summary: White-rot fungi have the ability to degrade various environmental pollutants, including pharmaceuticals. However, conventional wastewater treatment methods are not efficient in removing these contaminants. This study aims to find low-cost culture media for fungal cultures to optimize biomass production and enzyme activity. The results show that certain media variants composed of agricultural byproducts can promote fungal growth and enzyme production. Additionally, the study demonstrates the potential of using Pleurotus ostreatus in corn steep liquor medium to remove drug contaminants from water.
Review
Biochemistry & Molecular Biology
Aleksander Roszczyk, Jadwiga Turlo, Radoslaw Zagozdzon, Beata Kaleta
Summary: Shiitake mushroom, also known as Lentinula edodes, is a popular edible fungus that contains valuable polysaccharides with anticancer, antioxidant, antimicrobial, and immunomodulatory properties. The biological activity of these polysaccharides depends on the extraction method.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Medicinal
Valeria Napolitano, Piotr Mroz, Monika Marciniak, Vishal C. Kalel, Charlotte A. Softley, Julian D. Janna Olmos, Bettina G. Tippler, Kenji Schorpp, Sarah Rioton, Tony Froehlich, Oliver Plettenburg, Kamyar Hadian, Ralf Erdmann, Michael Sattler, Grzegorz M. Popowicz, Maciej Dawidowski, Grzegorz Dubin
Summary: Trypanosomiases are neglected tropical diseases caused by Trypanosoma (sub)species. The inhibition of the PEX5-PEX14 protein-protein interaction has shown potential as a lethal treatment for Trypanosoma. Researchers have developed a novel compound scaffold that inhibits PEX5-PEX14 PPI and has demonstrated trypanocidal activity in cell-based assays.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Aleksander Roszczyk, Michal Zych, Katarzyna Zielniok, Natalia Krata, Jadwiga Turlo, Marzenna Klimaszewska, Radoslaw Zagozdzon, Beata Kaleta
Summary: The polysaccharides isolated from Lentinula edodes, particularly the Se-Le-30 fraction, have shown immunomodulatory effects on human T lymphocytes by inhibiting proliferation of CD3-stimulated T cells and enhancing cytokine synthesis. These findings suggest the potential use of Se-Le-30 as a therapeutic compound in immunomodulation research.
Article
Chemistry, Medicinal
Piotr Stepnicki, Katarzyna M. Targowska-Duda, Anton L. Martinez, Agata Zieba, Olga Wronikowska-Denysiuk, Martyna Z. Wrobel, Agata Bartyzel, Alicja Trzpil, Tomasz M. Wrobel, Andrzej Chodkowski, Karolina Mirecka, Tadeusz Karcz, Katarzyna Szczepanska, Maria I. Loza, Barbara Budzynska, Jadwiga Turlo, Jadwiga Handzlik, Emilia Fornal, Ewa Poleszak, Marian Castro, Agnieszka A. Kaczor
Summary: Schizophrenia is a complex mental disorder involving various neurotransmitter systems. Antipsychotics can be divided into classical drugs and atypical antipsychotics, the latter of which have a multi-target profile affecting both dopamine and serotonin receptors. This profile is considered more effective and safer in treating symptoms.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Piotr Stepnicki, Olga Wronikowska-Denysiuk, Agata Zieba, Katarzyna M. Targowska-Duda, Agata Bartyzel, Martyna Z. Wrobel, Tomasz M. Wrobel, Klaudia Szalaj, Andrzej Chodkowski, Karolina Mirecka, Barbara Budzynska, Emilia Fornal, Jadwiga Turlo, Marian Castro, Agnieszka A. Kaczor
Summary: The study aims to find new antipsychotics by optimizing the compound D2AAK3, a multi-target ligand of G-protein-coupled receptors (GPCRs). Compounds 1 and 10 were selected based on their receptor profile and evaluated for their antipsychotic activity, as well as their effects on memory and anxiety processes. Molecular modelling and X-ray study were conducted to investigate the interactions and stable conformation of the compounds.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Medicinal
Monika Marciniak, Piotr Mroz, Valeria Napolitano, Vishal C. Kalel, Roberto Fino, Emilia Pykacz, Wolfgang Schliebs, Oliver Plettenburg, Ralf Erdmann, Michael Sattler, Grzegorz M. Popowicz, Maciej Dawidowski
Summary: Protein-protein interactions (PPIs) are important targets for small molecule drugs, but can be challenging to inhibit. In this study, a new class of peptidomimetic scaffolds was developed to target the PEX5-PEX14 PPI, which plays a critical role in glycosome biogenesis and is a potential target for drugs against Trypanosoma infections. The designed peptidomimetics inhibited the PEX5-TbPEX14 PPI and exhibited cellular activity against T. b. brucei, providing a promising approach for the development of trypanocidal agents and helical mimetics as PPI inhibitors.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Nutrition & Dietetics
Malgorzata Kalucka, Aleksander Roszczyk, Marzenna Klimaszewska, Beata Kaleta, Ewelina Drelich, Anna Blazewicz, Sandra Gorska-Jakubowska, Eliza Malinowska, Marek Krol, Aleksandra Maria Prus, Katarzyna Trzesniowska, Aleksandra Wolczynska, Przemyslaw Dorozynski, Radoslaw Zagozdzon, Jadwiga Turlo
Summary: In this study, mycelial cultures of Lentinula edodes were used to obtain selenium-containing immunomodulatory preparations. The researchers also aimed to investigate the simultaneous accumulation of selenium and zinc in these mycelia. The effects of the selenium and zinc extracts on T cell activation in human PBMCs were studied, and significant changes were observed in the expression of PD-1 and CD25 antigens on CD8+ T cells. The selenium and zinc content in the preparations modified the immunomodulatory activity of mycelial polysaccharides, but the mechanisms of action of the active ingredients in the extracts were found to be different.
Article
Biochemistry & Molecular Biology
Martyna Z. Wrobel, Andrzej Chodkowski, Maciej Dawidowski, Agata Siwek, Katarzyna Stachowicz, Bernadeta Szewczyk, Gabriel Nowak, Grzegorz Satala, Andrzej J. Bojarski, Jadwiga Turlo
Summary: The synthesis and evaluation of new compounds targeting the serotonin 1A receptors and serotonin transporter in the treatment of central nervous system diseases, especially depression, were conducted. Compound 4f emerged as the most promising with good activity, stability, and potential as a therapeutic agent.
BIOORGANIC CHEMISTRY
(2023)