4.7 Article

Combination of essential oil compounds and phenolic acids against Escherichia coli O157:H7 in vitro and in dry-fermented sausage production

Journal

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
Volume 260, Issue -, Pages 59-64

Publisher

ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijfoodmicro.2017.08.010

Keywords

Food safety; EHEC; allyl isothiocyanate; o-coumaric acid

Funding

  1. CNPq (Brazilian National Council for Scientific and Technological Development) [454294/2014-0]
  2. PUCPR
  3. University of Manitoba

Ask authors/readers for more resources

Escherichia coli O157:H7 is a foodborne pathogen that causes hemorrhagic colitis and hemolytic uremic syndrome. The low dose of infection and severity of the disease represent a concern to public health. Natural compounds have been widely applied as food additives to replace synthetic preservatives. The aim of this study was to determine the efficiency of essential oil compounds (EOCs) in combination with phenolic acids (PA) in vitro and in dry-fermented sausage production. Minimum Inhibitory Concentration (MIC) and Fractional Inhibitory Concentration index (FICindex) were determined for a 5-strain mixture of E. coli O157:H7. Batches of sausage tainted with E. coil O157:H7 were produced using Pediococcus pentosaceus UM 116P and Staphylococcus carnosus UM 123M as starter cultures. The best combination of EOCs and PAs found in vitro was used as an additive. Chemical-physical and microbiological analyses were evaluated weekly from day 0 to 35 after production. Sensory evaluation (texture, odor, flavor, appearance and overall evaluation) of E. coil-free sausages was conducted using a 9-point hedonic scale with 56 untrained volunteers. The MIC values of allyl isothiocyanate (AITC), carvacrol (CAR), ferulic acid (FA), o-coumaric acid (CA) and p-hydroxybenzoic acid (AHB) were, respectively, 0.25; 1.3; 5.12; 18.27; and 37 mM. AITC combined with CA had a synergistic effect (FICindex = 0.25) and together they were applied in the production of dry fermented sausage at concentrations of 10 x FIC and 20 x FIC. A(w) had no significant difference among treatments, whereas the pH of 10 x FIC and 20 x FIC were higher than the control. E. colt O157:H7 was reduced by > 5 log CFU/g with 20 x FIC after 21 d, and by 2.8 log CFU/g with 10 x FIC after 35 d. Sensory analysis showed that the combination of AITC and o-c oumaric acid in both treatments presented lower scores in the 5 categories when compared to the control, but none of the parameters received a negative score. This study demonstrated that the combination of AITC and o-coumaric acid at 20 x FIC reduced E. coli O157:H7 in compliance with the North American legislation, but adjustments in the dose are necessary to improve the sensory characteristics of the final product.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

Review Food Science & Technology

Non-conventional cultures and metabolism-derived compounds for bioprotection of meat and meat products: a review

Gabriela Maia Danielski, Alberto Goncalves Evangelista, Fernando Bittencourt Luciano, Renata Ernlund Freitas de Macedo

Summary: Meat and meat products are susceptible to pathogenic or spoilage bacteria, causing economic losses and public health threats. Non-conventional microorganisms and biometabolites have potential for bio protection, improving the quality and safety of meat products.

CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION (2022)

Review Food Science & Technology

The impact of essential oils on antibiotic use in animal production regarding antimicrobial resistance - a review

Alberto Goncalves Evangelista, Jessica Audrey Feijo Correa, Anne Caroline Schoch Marques Pinto, Fernando Bittencourt Luciano

Summary: Population growth affects global food supply by demanding higher production efficiency without expanding farmland due to limited land resources and biodiversity loss. The intense use of antibiotic growth promoters in animal production has been linked to antimicrobial resistance rise, leading to the emergence of multidrug-resistant pathogens and limiting treatment options. Essential oils and essential oil components have been studied as potential alternatives to antibiotics in animal production, showing antimicrobial effectiveness and potential for improving zootechnical indexes and modulating genes involved in resistance mechanisms.

CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION (2022)

Article Biotechnology & Applied Microbiology

Bacteriophages as tools for biofilm biocontrol in different fields

Camila Mendes Figueiredo, Marilia Silva Malvezzi Karwowski, Romeu Cassiano Pucci da Silva Ramos, Nicoly Subtil de Oliveira, Lorena Caroline Pena, Everdan Carneiro, Renata Ernlund Freitas de Macedo, Edvaldo Antonio Ribeiro Rosa

Summary: Microbial biofilms are challenging to control due to limited accessibility of antimicrobial drugs. This review discusses the advantages, recommendations, and limitations of using bacteriophages as 'biofilm predators', and the feasibility of using phages to control biofilms in different environments.

BIOFOULING (2021)

Article Food Science & Technology

Garlic essential oil as an antifungal and anti-mycotoxin agent in stored corn

Karla Paiva Bocate, Alberto Goncalves Evangelista, Fernando Bittencourt Luciano

Summary: The study confirmed the effectiveness of garlic essential oil in inhibiting fungal growth and mycotoxin contamination in stored corn kernels. Although garlic essential oil showed potential in reducing mycotoxin production by fungi, it was unable to directly react with mycotoxins during simulated digestion.

LWT-FOOD SCIENCE AND TECHNOLOGY (2021)

Article Food Science & Technology

Combined application of phenolic acids and essential oil components against Salmonella Enteritidis and Listeria monocytogenes in vitro and in ready-to-eat cooked ham

Jessica Audrey Feijo Correa, Joao Vitor Garcia dos Santos, Alberto Goncalves Evangelista, Anne Caroline Schoch Marques Pinto, Renata Ernlund Freitas de Macedo, Fernando Bittencourt Luciano

Summary: This study found that combinations of EOCs and PAs can have antimicrobial effects in food and significantly reduce the amount of EOC required. In experiments with sliced ham, it was discovered that CINN and HCA are the most effective combination for inhibiting the growth of foodborne pathogens.

LWT-FOOD SCIENCE AND TECHNOLOGY (2021)

Article Parasitology

Can orange essential oil reduce the severity of parasitic infection in sheep?

Maria Theresa Scheffer Pereira da Silva, Matheus Borges de Carvalho, Diogenes Adriano Duarte Santana, Sthefany Kamile dos Santos, Rudiger Daniel Ollhoff, Fernando Bittencourt Luciano, Cristina Santos Sotomaior

Summary: This study evaluated the effectiveness of orange essential oil to reduce parasitic infection severity in lambs naturally infected with gastrointestinal parasites. The results showed a significant decrease in egg count per gram of feces in the OEO-treated groups, especially in the second stage of the experiment. However, while OEO was able to reduce EPG by more than 96% on day 3, the effect did not persist in subsequent evaluations, indicating the necessity of further research to optimize the use of OEO in conjunction with other control methods.

VETERINARY PARASITOLOGY- REGIONAL STUDIES AND REPORTS (2021)

Article Agriculture, Dairy & Animal Science

Simultaneous feeding of calcium butyrate and tannin extract decreased the incidence of diarrhea and proinflammatory markers in weaned piglets

Camila Demarco Maito, Antonio Diego Brandao Melo, Angela Cristina da Fonseca de Oliveira, Jansller Luiz Genova, Jair Rodini Engracia Filho, Renata Ernlund Freitas de Macedo, Kelly Mazutti Monteiro, Saulo Henrique Weber, Astrid Koppenol, Leandro Batista Costa

Summary: This study investigated the effect of combining calcium butyrate with tannin extract on the growth performance, incidence of diarrhea, intestinal histology, and immune-expression in piglets. The results showed that the combination of calcium butyrate and tannin extract significantly reduced the incidence of diarrhea and inflammatory processes in the duodenum of piglets. Therefore, this combination could be used as an alternative to reduce the use of antimicrobials in the diet of weaned piglets.

ANIMAL BIOSCIENCE (2022)

Review Food Science & Technology

Carnobacterium as a bioprotective and potential probiotic culture to improve food quality, food safety, and human health - a scoping review

Alberto Goncalves Evangelista, Gabriela Maia Danielski, Jessica Audrey Feijo Correa, Caroline Maria de Andrade Cavalari, Isabelle Ramos Souza, Fernando Bittencourt Luciano, Renata Ernlund Freitas de Macedo

Summary: Carnobacterium bacteria have shown potential as probiotics and bioprotective agents, particularly in animal production and the food industry. However, research on human health applications is still in the early stages and there is a lack of in vivo studies, limiting its widespread use.

CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION (2023)

Article Microbiology

Cell-free supernatants produced by lactic acid bacteria reduce Salmonella population in vitro

Alberto Goncalves Evangelista, Jessica Audrey Feijo Correa, Joao Vitor Garcia dos Santos, Eduardo Henrique Custodio Matte, Monica Moura Milek, Gabrieli Camila Biauki, Leandro Batista Costa, Fernando Bittencourt Luciano

Summary: The study evaluated the use of lactic acid bacteria (LAB) cell-free supernatants (CFS) against Salmonella, showing that lyophilized CFS had significant antimicrobial activity against Salmonella both in vitro and in simulated animal digestion. The findings suggest that CFS could be a promising feed additive to control Salmonella in animal production as an alternative to antibiotics, especially in light of concerns about antimicrobial resistance.

MICROBIOLOGY-SGM (2021)

Correction Agriculture, Multidisciplinary

Fumigation of Brazil nuts with allyl isothiocyanate to inhibit the growth of Aspergillus parasiticus and aflatoxin production (vol 98, pg 792, 2018)

Lucas F. Lopes, Keliani Bordin, Gabriel H. C. de Lara, Federica Saladino, Juan M. Quiles, Giuseppe Meca, Fernando B. Luciano

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2022)

Article Agriculture, Dairy & Animal Science

New findings of intestinal alkaline phosphatase: effects on intestinal and organ health of piglets challenged with ETEC F4 (K88)

Jansller Luiz Genova, Antonio Diego Brandao Melo, Paulo Evaristo Rupolo, Renata Ernlund Freitas de Macedo, Jair Rodini Engracia, Silvana Teixeira Carvalho, Luigi Faucitano, Leandro Batista Costa, Paulo Levi de Oliveira Carvalho

Summary: This study aimed to assess the effects of adding intestinal alkaline phosphatase to diets on piglets challenged with Escherichia coli. The results showed that feeding 30 mg IAP inhibited Enterobacteriaceae population and suggested a potential effect in mitigating intestinal injuries.

REVISTA BRASILEIRA DE ZOOTECNIA-BRAZILIAN JOURNAL OF ANIMAL SCIENCE (2022)

Article Biology

Microbial Biodiversity in Honey and Pollen Pots Produced by Tetragonisca angustula (Jatai)

Marcia Regina Beux, Suelen Avila, Monica Surek, Keliani Bordin, Jaqueline Leobet, Fabiana Barbieri, Sila Mary Rodrigues Ferreira, Edvaldo Antonio Ribeiro Rosa

Summary: This study analyzed the microbial community in the nest pots of Tetragonisca angustula bees and found various bacteria and yeast species. Bacillus was the dominant bacteria in honey and pollen, while Z. bailli was the dominant yeast in pollen and S. meliponinorum was the dominant yeast in honey.

BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY (2022)

Article Agronomy

Nisin and ε-poly-L-lysine as natural antimicrobials towards spoilage-associated Lactobacillus plantarum

Fernanda Cristina Kandalski Bortolotto, Maria Helena da Rosa Farfan, Nathalia Cristina Kleinke Jede, Gabriela Maia Danielski, Renata Ernlund Freitas de Macedo

Summary: The study aimed to isolate lactic acid bacteria from spoiled commercial sausages and challenge them in vitro with natural antimicrobials. Results showed the potential of the combined use of nisin and epsilon-poly-L-lysine to control sausage spoilage.

CIENCIA RURAL (2021)

Article Food Science & Technology

Influence of sampling location and processing on the assembly and network of Polygoni Multiflori Radix surface microbiome

Guangfei Wei, Yichuan Liang, Guozhuang Zhang, Zhaoyu Zhang, Yongqing Zhang, Shilin Chen, Linlin Dong

Summary: This study investigated the presence of fungi on the surface of Polygonum multiflorum Thunb and found that it is easily contaminated by toxigenic fungi and mycotoxins, posing a health risk for consumers. The results also showed that sampling location and processing can influence the assembly and network complexity of the surface microbiome of Polygonum multiflorum Thunb.

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY (2024)

Article Food Science & Technology

The wooden shelf surface and cheese rind mutually exchange microbiota during the traditional ripening process

Gabriele Busetta, Giuliana Garofalo, Salvatore Claps, Maria Teresa Sardina, Elena Franciosi, Antonio Alfonzo, Nicola Francesca, Giancarlo Moschetti, Luca Settanni, Raimondo Gaglio

Summary: This study evaluates the microbial diversity of wooden shelves used for the ripening of PDO Pecorino di Filiano and PGI Canestrato di Moliterno cheeses. The results show a strict correlation between the microbiota of wooden shelves and cheese rinds, indicating the positive role of wooden shelves in the cheese ripening process.

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY (2024)

Article Food Science & Technology

Reduction of PDO Pecorino Siciliano cheese making duration: Microbial dynamics and quality attributes deriving from replacing whey permeate with hot water during cooking

Massimo Todaro, Giuliana Garofalo, Gabriele Busetta, Riccardo Gannuscio, Ambra Rita Di Rosa, Maria Luisa Scatassa, Cinzia Cardamone, Isabella Mancuso, Elena Franciosi, Fatima Rando, Monica Agnolucci, Vincenzo Chiofalo, Raimondo Gaglio, Luca Settanni

Summary: This study aimed to reduce the production time of PDO Pecorino Siciliano cheese by comparing two cooking methods and assessing their impact on microbiological composition and lactic acid bacteria populations. The results showed that the different cooking methods did not affect the physicochemical characteristics of the cheese, and sensory evaluation confirmed that the modified production protocol did not significantly alter the product characteristics and overall acceptance. Therefore, cooking under hot water proved to be an effective way to reduce the transformation duration while maintaining the typicality of PDO Pecorino Siciliano cheese.

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY (2024)

Article Food Science & Technology

Effects of ohmic heating with different voltages on the quality and microbial diversity of cow milk during thermal treatment and subsequent cold storage

Yingying Sun, Yana Liu, Weiwei Zhou, Lele Shao, Han Wang, Yijie Zhao, Bo Zou, Xingmin Li, Ruitong Dai

Summary: The study evaluates the effect of Ohmic heating (OH) at various voltage gradients and water bath on microbial inactivation, physicochemical and sensory properties, and microbial flora of pasteurized milk. Results show that OH with higher voltage effectively inactivates microorganisms, slows down lipid oxidation, and maintains sensory quality and amino acids content of milk. The microbial community in milk treated with OH remains relatively stable during storage.

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY (2024)

Article Food Science & Technology

Bacteriophages PECP14, PECP20, and their endolysins as effective biocontrol agents for Escherichia coli O157:H7 and other foodborne pathogens

Minjin Oh, Ana Cevallos-Urena, Byoung Sik Kim

Summary: In this study, two bacteriophages, PECP14 and PECP20, infecting Escherichia coli O157:H7 were isolated and found to be stable under different environmental conditions. These phages exhibited specificity in targeting different host receptors and demonstrated the ability to infect other foodborne pathogens. The efficacy of phage treatment was demonstrated through rapid adsorption and bacterial lysis. Furthermore, purified endolysins derived from these phages showed remarkable bacteriolytic activity not only against E. coli O157:H7 but also against other pathogens. These findings suggest that these phages and their endolysins can be used for biological control of E. coli O157:H7 in various stages of agricultural product production.

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY (2024)

Review Food Science & Technology

A systematic review on the occurrence of Salmonella in farmed Tenebrio molitor and Acheta domesticus or their derived products

Filippo Marzoli, Michela Bertola, Juliane Pinarelli Fazion, Giulia Cento, Pietro Antonelli, Beatrice Dolzan, Lisa Barco, Simone Belluco

Summary: Insects are a sustainable and protein-rich food source, and Salmonella is a common pathogen in insects. This systematic review aims to collect and evaluate existing studies on the occurrence of Salmonella in crickets and mealworms. Although the number of studies is limited and there are some limitations, this review highlights the importance of obtaining reliable data on the presence of Salmonella in insects, considering the growing market and increased farming scale.

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY (2024)

Review Food Science & Technology

Comparison of Listeria monocytogenes alternative detection methods for food microbiology official controls in Europe

Lucas Chikhi, Melanie Mancier, Hubert Brugere, Bertrand Lombard, Lyazrhi Faouzi, Laurent Guillier, Nathalie Gnanou Besse

Summary: Listeriosis is a severe foodborne disease with a high mortality rate, mainly caused by L. monocytogenes. The EU regulation sets qualitative or quantitative food safety criteria for L. monocytogenes and recommends the use of EN ISO 11290-1 as the reference detection method. Our study compares alternative certified methods for detecting L. monocytogenes, highlighting the importance of diversifying available detection methods.

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY (2024)

Article Food Science & Technology

Ultralow temperature high pressure processing enhances inactivation of norovirus surrogates

Christina A. M. DeWitt, Kevin A. Nelson, Hyung Joo Kim, David H. Kingsley

Summary: High pressure processing (HPP) is a non-thermal method that can effectively inactivate pathogens. Research has shown that applying high pressure to thawed samples in an ultra-low temperature environment enhances the inactivation of norovirus.

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY (2024)

Article Food Science & Technology

Comparison of molecular techniques for the detection of Toxoplasma gondii in raw bovine milk from small rural properties in Brazil

Suzane Manzini, Thaina Valente Bertozzo, Isabella Neves Aires, Nassarah Jabur Lot Rodrigues, Amanda Bezerra Bertolini, Marcela Alexandrino, Jackieline Sampaio Steinle, Renata Pimentel Bandeira de Melo, Rinaldo Aparecido Mota, Maria Izabel Merino de Medeiros, Virginia Bodelao Richini-Pereira, Vera Claudia Lorenzetti Magalhaes Curci, Simone Baldini Lucheis

Summary: This study detected Toxoplasma gondii DNA in raw bovine milk samples from expansion tanks in small properties located in different cities of the Midwest region of Sa similar to o Paulo, Brazil using PCR. The results showed that the TgNP1 and TgNP2 genes were more efficient in detecting T. gondii DNA, highlighting the importance of raw bovine milk as a potential source of human infections caused by this parasite, and emphasizing the need for proper pasteurization and further understanding of the epidemiology of this protozoan.

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY (2024)

Article Food Science & Technology

Salmonella in horses at slaughter and public health effects in Italy

Luca Bolzoni, Mauro Conter, Luca Lamperti, Erika Scaltriti, Marina Morganti, Antonio Poeta, Marco Vecchi, Silvia Paglioli, Alessandra Rampini, Paolo Ramoni, Daniela De Vita, Cristina Bacci, Martina Rega, Laura Andriani, Stefano Pongolini, Silvia Bonardi

Summary: This study assessed the role of equids as carriers of Salmonella and the occurrence of contaminated equid carcasses during the slaughter process. Salmonella was detected in a small percentage of equids' caecal contents, but not in carcass samples. Additionally, a genetic match was found between Salmonella strains from horse caecal contents and human cases, suggesting a link between horse meat consumption and salmonellosis.

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY (2024)

Article Food Science & Technology

Effect of multiple nonthermal plasma treatments of filamentous fungi on cellular phenotypic changes and phytopathogenicity

Daria Nowinski, Tomasz Czapka, Irena Maliszewska

Summary: The effect of multiple sublethal doses of non-thermal plasma treatments on phenotypical changes and the reduction in phytopathogenicity of three species of fungi was investigated. The plasma damaged the surface of the fungal hyphae, decreased their dry biomass, and affected the activity of pectinase and xylanase. Furthermore, the plasma treatments increased the germination rate of artificially infected cucumber seeds and resulted in plants with higher biomass and longer roots and stems. However, the reduction in phytopathogenicity was not complete after the multiple plasma treatments.

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY (2024)