Review
Biochemistry & Molecular Biology
Takashi Iyanagi
Summary: This article compares the roles of FNR-Fd and FNR-Fld complex pairs in plants with the diflavin reductase (FAD-FMN) family and discusses the evolutionary aspects of NAD(P)H-dependent multi-domain electron transfer systems.
Article
Biochemistry & Molecular Biology
Yoko Kimata-Ariga, Rena Morihisa
Summary: This study investigated the effects of DHA on the NADPH/FNR/ferredoxin redox cascade system and found that DHA can partially inhibit the activity of FNR but does not significantly affect the electron transfer activity from FNR to wild-type or D97Y mutant ferredoxin. Computer docking analysis suggested that DHA may bind to the binding cavity of FNR. The mutation in ferredoxin may be involved in the protective response against the oxidative stress caused by artemisinin.
Article
Plant Sciences
Magda Grabsztunowicz, Marjaana Rantala, Aiste Ivanauskaite, Tiina Blomster, Minna M. Koskela, Katariina Vuorinen, Esa Tyystjarvi, Meike Burow, Kirk Overmyer, Ari P. Mahonen, Paula Mulo
Summary: The study reveals the distinct expression patterns and accumulation locations of RFNR1 and RFNR2 in response to different stresses in Arabidopsis, indicating their essential roles in plant development and survival. Although the functions of RFNR1 and RFNR2 are partially redundant, both proteins are crucial for plant growth and adaptation to environmental challenges.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Biochemistry & Molecular Biology
Yutaro Chikuma, Masayuki Miyata, Young-Ho Lee, Toshiharu Hase, Yoko Kimata-Ariga
Summary: This study investigated the molecular mechanism of negative cooperativity between FNR and Fd in plants, caused by the allosteric binding of NADP(H) on FNR. It identified a salt bridge between Asp60 of Fd and Lys33 of FNR as crucial for this negative cooperativity. Site-specific mutants of FdD60 and FNRK33 confirmed the involvement of this salt bridge in downregulating the interaction between FNR and Fd by NADPH.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)
Article
Microbiology
Neil T. Miller, Ghada Ajlani, Robert L. Burnap
Summary: Ferredoxin:NADP-oxidoreductase (FNR) plays a crucial role in photosynthetic electron transfer and respiration. The cyanobacterium Synechocystis sp. PCC6803 possesses two isoforms of FNR: FNRL and FNRS. The study found that under stressful conditions, the FNRS isoform contributes to increased cyclic electron flow and proton pumping.
Article
Biochemistry & Molecular Biology
Paula Monchietti, Arleth S. Lopez Rivero, Eduardo A. Ceccarelli, Daniela L. Catalano-Dupuy
Summary: FNRs are ubiquitous flavoenzymes involved in redox metabolisms, catalyzing electron transfer between NADP(H) and ferredoxin or flavodoxin. Plant-type FNRs are divided into plastidic and bacterial classes, with plastidic FNRs showing higher turnover numbers. Research on bacterial FNRs revealed a new model of NADP(+) binding and catalysis, highlighting its essential role in redox homeostasis.
Article
Agronomy
Yingchao Shen, Adhimoolam Karthikeyan, Yunhua Yang, Na Ma, Jinlong Yin, Yuan Yuan, Liqun Wang, Haijian Zhi
Summary: This study investigated the functional response of the soybean FNR gene during soybean mosaic virus (SMV) infection by silencing the gene in resistant and susceptible lines. The silencing of GmFNR resulted in decreased photosynthetic capacity and catalase activity in both lines, with a significant increase in H2O2 content in the susceptible line. SMV infection in the susceptible line caused serious morphological changes and increased SMV NIa-protease transcript accumulation; however, this impact was not observed in the resistant line.Analysis revealed that GmFNR interacts with EF1A and coincides with increased SMV accumulation, providing novel insights into the SMV resistance mechanism.
Article
Chemistry, Multidisciplinary
Christine M. Lewis, Justin D. Flory, Thomas A. Moore, Ana L. Moore, Bruce E. Rittmann, Wim F. J. Vermaas, Cesar Torres, Petra Fromme
Summary: A novel microbial electro-photosynthetic system (MEPS) is presented, which uses redox mediators and electrodes to drive electron transport in live cells. The results show that light-dependent current can be generated at high light intensities, and the electron delivery rate exceeds that of wildtype photosystem II (PSII). Furthermore, electron delivery occurs before the cytochrome b(6)f complex, enabling the production of both NADPH and ATP.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
Yoko Kimata-Ariga, Karen Fukuta, Masayuki Miyata
Summary: In the chloroplast stroma, changes in pH affect the electron transfer activity between ferredoxin-NADP(+) reductase (FNR) and ferredoxin (Fd) isoproteins in maize leaves. Increasing pH leads to increased activity between FNR and photosynthetic-type Fd (Fd1), while activity with nonphotosynthetic type Fd (Fd3) decreases. The His78 amino acid residue in Fd1 plays a regulatory role in its interaction with FNR, as demonstrated by mutation studies.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2022)
Article
Microbiology
Wanwipa Ittarat, Takeshi Sato, Masaharu Kitashima, Hidehiro Sakurai, Kazuhito Inoue, Daisuke Seo
Summary: Through studying the oxidoreductases in the genome of the green sulfur bacterium, it was found that rubredoxin (Rd) and a putative flavodiiron protein (FDP) may participate in the reduction of dioxygen, and the ferredoxin-NAD(P)(+) oxidoreductase (FNR) can promote the reduction of Rd.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Biophysics
Stanislav D. Zakharov, Sergei Savikhin, Yuko Misumi, Genji Kurisu, William A. Cramer
Summary: This study confirms the absence of a significant interaction between FNR and the b(6)f complex through thermodynamic analysis. It also provides a precedent for considering protein-detergent interactions in ITC analyses.
BIOPHYSICAL JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Hitesh Medipally, Marvin Mann, Carsten Koetting, Willem J. H. van Berkel, Marc M. Nowaczyk
Summary: Photosynthetic organisms use light for the regeneration of NADPH. In this study, a clickable fusion system was established to regenerate NADPH with PSI-Fd and PSI-Fd-FNR. Immunity protein 7 was fused to the PSI-PsaE subunit, and colicin DNase E7 fusion chimeras of Fd and FNR were expressed. The complexes showed the ability to regenerate NADPH, demonstrating the applicability of this strategy.
Article
Biochemistry & Molecular Biology
H. Doerpholz, S. Subramanian, A. Zouni, F. Lisdat
Summary: In this study, photobioelectrodes based on a ferredoxin-modified photosystem I (PSI-Fd) from Thermosynechococcus vestitus were prepared and characterized. The direct electron transfer between PSI-Fd and the electrode was investigated. The results showed that the modified PSI-Fd photobioelectrode had a lower photocurrent and a lower onset potential of the cathodic photocurrent compared to native PSI, mainly due to the different adsorption behavior of the PSI-Fd construct onto the 3D ITO electrode. However, the overall behavior was similar to PSI. First experiments demonstrated the usage of the PSI-Fd photobioelectrode for enzyme-driven NADPH generation.
BIOELECTROCHEMISTRY
(2023)
Article
Chemistry, Physical
Ayshat M. Bozieva, Makhmadyusuf Kh. Khasimov, Roman A. Voloshin, Maria A. Sinetova, Elena V. Kupriyanova, Sergey K. Zharmukhamedov, Dmitry O. Dunikov, Anatoly A. Tsygankov, Tatsuya Tomo, Suleyman I. Allakhverdiev
Summary: This study aims to investigate the H2-releasing ability of new strains of cyanobacteria and evaluate the effects of the photosystem II inhibitor DCMU on H2 production under light and dark conditions. The results demonstrate that cultures treated with DCMU produced several times more H2 than untreated cells. The highest H2 photoproduction rate of 4.24 mmol H2 (mg Chl a h)-1 was observed in Dolichospermum sp. IPPAS B.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2023)
Article
Chemistry, Multidisciplinary
Yuxi Lin, So-Hyeon Park, Eugene Bok, Yunseok Heo, Seong-Bin Yang, Yoon-Sun Yi, Jun-Hyuck Lee, Donghyun Seo, Eunae Jo, Sungsu Lim, Yun Kyung Kim, Jozsef Kardos, Kyoung-Seok Ryu, Jaekwang Kim, Jooho Park, Young-Ho Lee
Summary: This study synthesized a novel amphiphilic sub-nanosized material, RR-BA, which showed specific binding to α SN through electrostatic interactions and induced amyloid fibrillation. RR-BA had no noticeable effect on the amyloid formation of K18 and Aβ42 due to weak and non-specific interactions.
Article
Biophysics
Led Yered Jafet Garcia Montes de Oca, Tecilli Cabellos Avelar, Gerardo Ignacio Picon Garrido, Alicia Chagoya-Lopez, Luis Gonzalez de la Vara, Norma Laura Delgado Buenrostro, Yolanda Irasema Chirino-Lopez, Carlos Gomez-Lojero, Emma Berta Gutierrez-Cirlos
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
(2016)
Article
Plant Sciences
Erika Mellado-Mojica, Luis E. Gonzalez de la Vara, Mercedes G. Lopez
Article
Plant Sciences
Barbara Lino, Alicia Chagolla, Luis E. Gonzalez de la Vara
Article
Biochemistry & Molecular Biology
Martin Vargas-Suarez, Alina Castro-Sanchez, Gabriela Toledo-Ortiz, Luis E. Gonzalez de la Vara, Elpidio Garcia, Herminia Loza-Tavera
Article
Plant Sciences
Flor C. Alcantar-Aguirre, Alicia Chagolla, Axel Tiessen, John Paul Delano, Luis Eugenio Gonzalez de la Vara
Article
Multidisciplinary Sciences
Gabriela Casique-Arroyo, Norma Martinez-Gallardo, Luis Gonzalez de la Vara, John P. Delano-Frier
Article
Cell Biology
Gerson N. Hernandez-Acevedo, Oscar H. Lopez-Portales, Darely Y. Gutierrez-Reyna, Erick Cuevas-Fernandez, Linda A. Kempis-Calanis, Rosario G. Labastida-Conde, Oscar B. Aguilar-Luviano, Oscar Ramirez-Pliego, Salvatore Spicuglia, Barbara Lino-Alfaro, Alicia Chagolla-Lopez, Luis E. Gonzalez-de la Vara, Maria Angelica Santana
JOURNAL OF CELLULAR PHYSIOLOGY
(2019)
Article
Entomology
A. N. Lule-Chavez, E. E. Avila, L. E. Gonzalez-de-la-Vara, M. A. Salas-Marina, J. E. Ibarra
NEOTROPICAL ENTOMOLOGY
(2019)
Article
Multidisciplinary Sciences
Jose E. Guzman-Flores, Lidia Steinemann-Hernandez, Luis E. Gonzalez de la Vara, Marina Gavilanes-Ruiz, Tony Romeo, Adrian F. Alvarez, Dimitris Georgellis
Article
Agronomy
Angel R. Flores-Sosa, Diana Soto-Magana, Luis E. Gonzalez-de la Vara, Lino Sanchez-Segura, Moustapha Bah, Dulce M. Rivera-Pastrana, Gerardo M. Nava, Edmundo M. Mercado-Silva
Summary: Research suggests that blackberry color reversion may be related to a reduction in anthocyanin content and fruit vibration-induced damage, leading to changes in anthocyanin intermolecular interactions in reverted fruit. Further studies are needed to validate this hypothesis.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Diego Andres Castro-Lopez, Luis E. Gonzalez de la Vara, Moises Santillan, Agustino Martinez-Antonio
Summary: This study proposes a new biotechnological working model for tryptophan production, which incorporates the activity of tryptophan secretion systems and genetic modifications made in two E. coli strains. The resulting deterministic model is able to simulate the tryptophan production levels achieved by genetically engineered E. coli strains in experiments.
FERMENTATION-BASEL
(2022)
Meeting Abstract
Immunology
G. N. Hernndez Acevedo, O. H. Lopez Portales, D. Y. Gutierrez Reyna, E. Cuevas Fernandez, L. Kempis Calanis, R. G. Labastida Conde, O. B. Aguilar Luviano, O. Ramirez Pliego, S. Spicuglia, B. Lino-Alfaro, A. Chagolla Lopez, L. E. Gonzlez de la Vara, M. A. Santana
EUROPEAN JOURNAL OF IMMUNOLOGY
(2019)
Article
Multidisciplinary Sciences
Jesus Barrera-Rojas, Luis Gonzalez de la Vara, Emmanuel Rios-Castro, Lourdes E. Leyva-Castillo, Carlos Gomez-Lojero
Article
Biotechnology & Applied Microbiology
Rafael Sentandreu, Antonio Caminero, Itzel Renteria, Claudia Leon-Ramirez, Luis Gonzalez-de-la-Vara, Eulogio Valentin-Gomez, Jose Ruiz-Herrera
FEMS YEAST RESEARCH
(2018)
Article
Biochemistry & Molecular Biology
Kun He, Mengyi Xie, Weifeng Hong, Yonghe Li, Yaolin Yin, Xiaojin Gao, Yi He, Yu Chen, Chuan You, Jingdong Li
Summary: Centromere protein L (CENPL) is overexpressed in hepatocellular carcinoma (HCC) and is associated with poor patient prognosis. Upregulation of CENPL promotes tumor proliferation and glycolysis in HCC cells by activating the MEK1/2-ERK1/2 signaling pathway.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Yingzi Wang, Haozhong Huang, Huimin Weng, Chunsen Jia, Bin Liao, Yang Long, Fengxu Yu, Yongmei Nie
Summary: Talin protein plays a crucial role in transmitting mechanical forces by connecting the extracellular matrix to the cytoskeleton. It converts mechanical signals into biochemical signals and serves as diagnostic, therapeutic, and prognostic indicators in diseases.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Review
Biochemistry & Molecular Biology
Hema Saranya Ilamathi, Marc Germain
Summary: Mitochondria are the central metabolic hubs in cells, relying on proteins encoded by nuclear DNA and mitochondrial DNA (mtDNA) for their function. The maintenance and distribution of mtDNA are crucial for proper mitochondrial function and are associated with mitochondrial diseases. mtDNA is organized into nucleoids that dynamically move and interact with each other. The replication and distribution of mtDNA nucleoids are regulated by the complex interplay of mitochondrial dynamics, ER-mitochondria contact sites, and cytoskeletal networks.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
K. K. Sruthi, Sirisha Natani, Ramesh Ummanni
Summary: The overexpression of TPD52 is associated with the emergence of neuroendocrine prostate cancer (NEPC). TPD52 activates the NF-kappa B - STAT3 axis to induce neuroendocrine differentiation (NED) of prostate cancer cells. Therapeutic targeting of TPD52 is important for the treatment of prostate cancer.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Cui Li, Feifan Xiang, Yuqi Gong, Yi Fu, Ge Chen, Zhi Wang, Zhong Li, Daiqing Wei
Summary: Our study demonstrates the significant role of tumor-derived microparticles (T-MPs) in osteosarcoma metastasis and immune response. T-MPs promote macrophage polarization and stimulate migration and chemoresistance of osteosarcoma cells, thereby affecting the progression of osteosarcoma.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)