Review
Oncology
Giuseppe Schepisi, Chiara Casadei, Ilaria Toma, Giulia Poti, Maria Laura Iaia, Alberto Farolfi, Vincenza Conteduca, Cristian Lolli, Giorgia Ravaglia, Nicole Brighi, Amelia Altavilla, Giovanni Martinelli, Ugo De Giorgi
Summary: This review discusses the current state of research in the field of immunotherapy for gynecological cancers, with a focus on CAR-T cell therapy development. Gynecological tumors have high incidence and mortality rates, with limited efficacy of conventional chemotherapy, but immunotherapy and CAR technology offer new treatment possibilities for these cancer types.
Review
Immunology
Bu-Fan Xiao, Jing-Tao Zhang, Yu-Ge Zhu, Xin-Run Cui, Zhe-Ming Lu, Ben-Tong Yu, Nan Wu
Summary: CAR-T cell therapy has shown significant clinical responses in hematological malignancies and is now being evaluated for treating solid tumors. Challenges in using CAR-T cells for lung cancer treatment include on-target, off-tumor toxicity, scarcity of tumor-specific antigen targets, T cell exhaustion, and low immune cell infiltration levels. Advances in tumor immunology and cell product manufacturing are driving the clinical translation of CAR-T cell therapy for lung cancer.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Oncology
Wenwen Wei, Dong Yang, Xi Chen, Dandan Liang, Liqun Zou, Xudong Zhao
Summary: This review summarizes the characteristics of non-B-cell acute leukemia and the efficacy and challenges of CAR-T cell therapy in treating this type of leukemia.
FRONTIERS IN ONCOLOGY
(2022)
Review
Oncology
Elien De Bousser, Nico Callewaert, Nele Festjens
Summary: T cell-engaging immunotherapy aims to activate cytotoxic T cells to destroy cancer cells, while CAR T cell therapy redirects immune cells to recognize tumor antigens. Despite success, challenges such as toxicities and limited efficacy need to be addressed for the broad use of CAR T cell therapy. Research is ongoing to develop more powerful CAR T cells.
Review
Immunology
Hao Zhang, Shuangli Zhu, Wanjun Deng, Rui Li, Haiting Zhou, Huihua Xiong
Summary: CAR-T cell therapy is a revolutionary adoptive cell therapy that has shown unprecedented progress in hematological tumors and has the potential to be an effective strategy for solid cancers. However, the therapy faces challenges such as the immunosuppressive tumor microenvironment and heterogeneous tumor antigens when used against solid cancers.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ruediger Klapdor, Shuo Wang, Michael A. Morgan, Katharina Zimmermann, Jens Hachenberg, Hildegard Buening, Thilo Doerk, Peter Hillemanns, Axel Schambach
Summary: Ovarian cancer stem cells with chemoresistance contribute to tumor recurrence, leading to poor survival rates. Targeting CD44 using CAR immunotherapy demonstrates potent cytotoxic activity against CD44-positive ovarian cancer cells. Furthermore, combined treatment of CD44-targeted therapy and cisplatin shows higher anti-tumor activity compared to sequential treatment.
Review
Oncology
Sara Toulouie, Gary Johanning, Yihui Shi
Summary: CAR T-cell therapy is a promising immunotherapy, most effective in patients with intact immune systems. ACT using engineered T-cells has shown significant efficacy, especially in anti-CD19 CAR T-cell therapy for B-cell lymphoma.
Review
Immunology
Peng Zhang, Yang Zhang, Nan Ji
Summary: Glioblastoma (GBM) is a deadly brain cancer with limited efficacy of standard treatments, necessitating the development of new therapies. Chimeric antigen receptor T (CAR-T) cell immunotherapy has shown success in hematological malignancies, but has not yet yielded promising results in GBM. CAR-T cell therapy for GBM faces challenges including tumor heterogeneity, immunosuppressive microenvironment, and cell persistence.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Stacie Shiqi Wang, Kylie Luong, Fiona Margaret Gracey, Shereen Jabar, Brad McColl, Ryan Stanley Cross, Misty Rayna Jenkins
Summary: CAR T cell therapy re-engineers patient-derived T cells to express a specific receptor targeting tumor antigens. Studies have shown that pMHCI-specific CARs can effectively recognize and kill tumor cells, forming a TCR-like immune synapse that enhances killing kinetics.
Review
Cell Biology
Tahereh Soltantoyeh, Behnia Akbari, Amirali Karimi, Ghanbar Mahmoodi Chalbatani, Navid Ghahri-Saremi, Jamshid Hadjati, Michael R. Hamblin, Hamid Reza Mirzaei
Summary: Metastatic melanoma is a highly aggressive and difficult to treat type of skin cancer, but new therapies like CAR T cell therapy offer hope for patients. However, challenges such as off-target toxicity and therapy resistance need to be addressed through combination approaches.
Review
Biochemical Research Methods
Cuilin Zhang, Qiuyu Zhuang, Jingfeng Liu, Xiaolong Liu
Summary: Synthetic biology is an interdisciplinary research area that uses engineering principles to design and construct biological systems for practical applications. Chimeric antigen receptor (CAR) T cells, as one of the most successful clinical applications of synthetic biology, have shown tremendous success in treating blood malignancies. However, there are still limitations to CAR T cell therapy, hence the need for innovative CAR design becomes urgent.
ACS SYNTHETIC BIOLOGY
(2022)
Review
Medicine, General & Internal
Zhihuan Yang, Ying Wang
Summary: Cellular therapies, particularly CAR-T cell therapy, have revolutionized the treatment of hematological malignancies. Both China and the United States have made significant contributions to the development of clinical trials for CAR-T cell therapy. However, there are still limitations such as high relapse rates and adverse side effects. Different approaches are being explored in clinical trials to address these issues and promising breakthroughs have been made.
CHINESE MEDICAL JOURNAL
(2023)
Article
Oncology
Allyson C. Banville, Maartje C. A. Wouters, Ann L. Oberg, Krista M. Goergen, Matthew J. Maurer, Katy Milne, Jahanshah Ashkani, Emma Field, Chanel Ghesquiere, Steven J. M. Jones, Matthew S. Block, Brad H. Nelson
Summary: In the majority of cases of HGSC, CA125, MSLN, and FOLRA are overexpressed at the transcriptional level, but MSLN and FOLRA also show significant expression in healthy tissues. At the protein level, CA125 has the highest expression proportion, followed by MSLN and FOLRA. The combination of CA125 and/or MSLN is the most promising, with over 90% of tumor cells expressing one or both antigens in 51.9% of cases.
GYNECOLOGIC ONCOLOGY
(2021)
Review
Pharmacology & Pharmacy
Taewoong Choi, Yubin Kang
Summary: Although treatment outcomes for multiple myeloma patients have greatly improved in the past two decades, the disease remains incurable. New immunotherapies, including monoclonal antibodies, antibody-drug conjugates, bispecific antibodies, and chimeric antigen receptor (CAR) T cell therapy, have emerged to treat multiple myeloma. This article provides a comprehensive review of the clinical efficacy, safety, and potential resistance mechanisms of current myeloma CAR-T therapies, with a focus on B Cell Maturation Antigen (BCMA) as the most successful target. The article also discusses novel strategies to enhance the effectiveness of myeloma CAR-T therapy.
PHARMACOLOGY & THERAPEUTICS
(2022)
Article
Medicine, Research & Experimental
Yizhao Chen, Xiangling Zhu, Hanze Liu, Cunzhi Wang, Yu Chen, Huihui Wang, Yilong Fang, Xuming Wu, Yuting Xu, Chunhua Li, Xinyue Lv, Jinghua Huang, Xintong Han, Ruilin Li, Wenming Hong, Zhiying Yu, Wei Wei, Jiajie Tu
Summary: This study constructed CAR-Ms targeting HER2 and CD47, demonstrating their ability to phagocytose ovarian cancer cells and activate CD8+ cytotoxic T lymphocytes. In vivo models showed that CAR-Ms enhanced CD8+ T cell activation and led to tumor regression by affecting the tumor microenvironment.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Oncology
Xiao-Jun Xu, De-Gang Song, Mathilde Poussin, Qunrui Ye, Prannda Sharma, Alba Rodriguez-Garcia, Yong-Min Tang, Daniel J. Powell
Article
Oncology
Sarah B. Gitto, Hyoung Kim, Stavros Rafail, Dalia K. Omran, Sergey Medvedev, Yasuto Kinose, Alba Rodriguez-Garcia, Ahron J. Flowers, Haineng Xu, Lauren E. Schwartz, Daniel J. Powell, Fiona Simpkins
GYNECOLOGIC ONCOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Alba Rodriguez-Garcia, Prannda Sharma, Mathilde Poussin, Alina C. Boesteanu, Nicholas G. Minutolo, Sarah B. Gitto, Dalia K. Omran, Matthew K. Robinson, Gregory P. Adams, Fiona Simpkins, Daniel J. Powell
Article
Chemistry, Multidisciplinary
Nicholas G. Minutolo, Prannda Sharma, Mathilde Poussin, Lauren C. Shaw, Daniel P. Brown, Erin E. Hollander, Anze Smole, Alba Rodriguez-Garcia, James Z. Hui, Fabiana Zappala, Andrew Tsourkas, Daniel J. Powell
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2020)
Review
Immunology
Alba Rodriguez-Garcia, Asis Palazon, Estela Noguera-Ortega, Daniel J. Powell, Sonia Guedan
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Multidisciplinary Sciences
Alba Rodriguez-Garcia, Rachel C. Lynn, Mathilde Poussin, Monika A. Eiva, Lauren C. Shaw, Roddy S. O'Connor, Nicholas G. Minutolo, Victoria Casado-Medrano, Gonzalo Lopez, Takami Matsuyama, Daniel J. Powell
Summary: The study demonstrates that targeting FR beta-expressing TAMs with CAR-T cells can enhance antitumor immune responses and improve the efficacy of adoptive T-cell therapy in pre-clinical models.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Allison G. Roy, J. Michael Robinson, Prannda Sharma, Alba Rodriguez-Garcia, Mathilde A. Poussin, Cheryl Nickerson-Nutter, Daniel J. Powell
Summary: FR beta is a potential target for cancer therapy, as demonstrated by high expression levels on malignant blasts in AML and M2 polarized TAMs in solid tumors. The monoclonal antibody m909 is effective in causing ADCC against FR beta-expressing cells, leading to tumor growth inhibition in AML mouse models and cytotoxicity to TAMs in ovarian cancer microenvironment. Further investigation of m909 and its derivatives as therapeutic agents for FR beta-expressing cancers is warranted.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Adam J. Wolpaw, Liron D. Grossmann, Jessica L. Dessau, May M. Dong, Bailey J. Aaron, Patricia A. Brafford, Darya Volgina, Guillem Pascual-Pasto, Alba Rodriguez-Garcia, Yasin Uzun, Marie Arsenian-Henriksson, Daniel J. Powell, Kristopher R. Bosse, Andrew Kossenkov, Kai Tan, Michael D. Hogarty, John M. Maris, Chi V. Dang
Summary: By investigating inflammatory signaling pathways in neuroblastoma, researchers have identified heterogeneity in dsRNA sensing and intratumoral inflammatory signaling, which has significant implications for immunotherapeutic strategies in this aggressive childhood cancer.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Oncology
Miriam Bazan-Peregrino, Rocio Garcia-Carbonero, Berta Laquente, Rafael Alvarez, Ana Mato-Berciano, Marta Gimenez-Alejandre, Sara Morgado, Alba Rodriguez-Garcia, Maria Maliandi, M. Carmen Riesco, Rafael Moreno, Mireia M. Ginesta, Mercedes Perez-Carreras, Joan B. Gornals, Susana Prados, Sofia Perea, Gabriel Capella, Ramon Alemany, Ramon Salazar, Emma Blasi, Carmen Blasco, Manel Cascallo, Manuel Hidalgo
Summary: The study showed that VCN-01 can replicate in PDAC models and improve antitumor effects when combined with chemotherapy. The hyaluronidase produced by VCN-01 degrades tumor stroma and facilitates the delivery of various therapeutic agents. Clinically, VCN-01 treatment was well-tolerated and resulted in disease stabilization of injected lesions.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Review
Oncology
Sonia Guedan, Maik Luu, Delphine Ammar, Paula Barbao, Chiara Bonini, Philippe Bousso, Christian J. Buchholz, Monica Casucci, Biagio De Angelis, Emmanuel Donnadieu, David Espie, Beatrice Greco, Richard Groen, Johannes B. Huppa, Chahrazade Kantari-Mimoun, Bruno Laugel, Mary Mantock, Janet L. Markman, Emma Morris, Concetta Quintarelli, Michael Rade, Kristin Reiche, Alba Rodriguez-Garcia, Juan Roberto Rodriguez-Madoz, Eliana Ruggiero, Maria Themeli, Michael Hudecek, Ibtissam Marchiq
Summary: Immunotherapy with gene engineered CAR and TCR transgenic T-cells is a groundbreaking treatment in cancer medicine, and has the potential to be used in common solid tumors. However, in order to improve and accelerate the selection of lead T-cell products for clinical translation, it is necessary to assess preclinical models and develop new models with higher predictive value.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Hematology
Lauren C. Shaw, Mathilde Poussin, Alba Rodriguez-Garcia, Joshua Eggold, Nicholas G. Minutolo, Jie Wang, Alain H. Rook, Stephen J. Schuster, Daniel J. Powell Jr
Summary: Peripheral T-cell lymphomas (PTCLs) are a heterogeneous group of lymphoid malignancies with ineffective treatment options and high relapse rates. Chimeric antigen receptor T-cell (CART) therapy has shown success in certain hematologic malignancies and presents a potential treatment option for PTCLs. Our study focuses on developing a CART platform specific to a given T-cell receptor v beta (TCRv beta) family, aiming to selectively target malignant cells while preserving healthy T cells. Our results demonstrate that TCRv beta-family-specific CARTs effectively kill TCRv beta-expressing target cells and eliminate the dominant malignant clone in patient samples, offering a high-precision treatment option for PTCL with limited healthy T-cell depletion.
Article
Oncology
Anze Smole, Alexander Benton, Mathilde A. Poussin, Monika A. Eiva, Claudia Mezzanotte, Barbara Camisa, Beatrice Greco, Prannda Sharma, Nicholas G. Minutolo, Falon Gray, Adham S. Bear, Miren L. Baroja, Casey Cummins, Chong Xu, Francesca Sanvito, Andrea Lang Goldgewicht, Tatiana Blanchard, Alba Rodriguez-Garcia, Michael Klichinsky, Chiara Bonini, Carl H. June, Avery D. Posey Jr, Gerald P. Linette, Beatriz M. Carreno, Monica Casucci, Daniel J. Powell Jr
Summary: In this study, a genetic platform called Uni-Vect was developed to combine CAR-T cells with the expression of accessory molecules, providing a foundation for a more clinically actionable cellular immunotherapy.
Meeting Abstract
Biotechnology & Applied Microbiology
Alba Rodriguez-Garcia, Prannda Sharma, Mathilde Poussin, Matthew K. Robinson, Sarah B. Gitto, Sergey Medvedev, Gregory P. Adams, Fiona Simpkins, Daniel J. Powell
Meeting Abstract
Biotechnology & Applied Microbiology
Alba Rodriguez-Garcia, Rachel C. Lynn, Takami Matsuyama, Daniel J. Powell
Article
Oncology
Dimitrios Nasioudis, Stefan Gysler, Nawar Latif, Lory Cory, Robert L. Giuntoli II, Sarah H. Kim, Fiona Simpkins, Lainie Martin, Emily M. Ko
Summary: The prevalence of ERBB2 gene amplification was investigated among patients with gynecologic malignancies. The study found that ERBB2 amplification is frequently encountered in uterine serous carcinoma and mucinous ovarian carcinoma, but less common in endometrioid endometrial carcinoma.
GYNECOLOGIC ONCOLOGY
(2024)