Article
Biochemistry & Molecular Biology
Peter S. Harris, Courtney D. McGinnis, Cole R. Michel, John O. Marentette, Richard Reisdorph, James R. Roede, Kristofer S. Fritz
Summary: In the U.S., alcohol-associated liver disease (ALD) is a significant healthcare burden, but the molecular mechanisms underlying ethanol hepatotoxicity are not fully understood. The metabolism of ethanol leads to disruptions in metabolic processes and oxidative stress. The reduction of cysteine residues and the impact on various pathways and protein targets reveals the importance of studying the proteome and post-translational modifications to develop therapeutic agents for ALD.
Article
Chemistry, Multidisciplinary
Leona L. Rodrigues, Aaron S. Micallef, Michael C. Pfrunder, Vinh X. Truong, John C. McMurtrie, Tim R. Dargaville, Anja S. Goldmann, Florian Feist, Christopher Barner-Kowollik
Summary: A highly efficient ligation system based on visible light-induced rearrangement was introduced, generating a thiophenol for rapid thiol-Michael additions without the need for additives. The self-catalyzed ligation through pyridine mediated deprotonation of the photochemically generated thiophenol was described, leading to additive-free thiol-Michael reactions. The study explored various reaction conditions, solvents, and substrates, showcasing excellent end group fidelity in polymer modification.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Review
Biochemistry & Molecular Biology
Nicholas J. Day, Matthew J. Gaffrey, Wei-Jun Qian
Summary: The review highlights how mass spectrometry-based redox proteomics has enabled accurate quantification of reversible oxidative modifications on specific cysteine residues of proteins, emphasizing the significance and application of such measurements in the field of redox biology.
Review
Biochemical Research Methods
Xiaolu Li, Austin Gluth, Tong Zhang, Wei-Jun Qian
Summary: Redox post-translational modifications greatly impact protein structure and function, allowing for the regulation of biological processes. Redox proteomics approaches use mass spectrometry to study these modifications and have contributed to our understanding of redox signaling and regulation. Various strategies, including blocking free thiols and using selective reduction and enrichment techniques, are employed to analyze redox PTMs. This review discusses the principles and recent advances in redox proteomics and highlights its applications in generating novel biological insights.
Article
Chemistry, Physical
Guoneng Chen, Riyan Lin, Yanshi Lei, Peng Cai, Yifu Huang, Hefeng Zhang
Summary: In this study, a new spiropyran-containing polyurethane ionogel with photochromic, photomechanical, and photoconductive properties was successfully prepared through thiol-ene chemistry. It was found that the participation of the ring-opened spiropyran in the thiol-ene reaction affected its reverse photochromic behavior.
Review
Biochemistry & Molecular Biology
Lisa R. Knoke, Lars I. Leichert
Summary: Due to its nucleophilicity, the thiol group of cysteine plays an important role in protein chemistry. The nucleophilicity of the thiol group makes cysteine susceptible to oxidative modifications, which can alter protein function and are reversible in vivo. Therefore, studying oxidative posttranslational modifications of cysteine is crucial for understanding redox biology.
CURRENT OPINION IN CHEMICAL BIOLOGY
(2023)
Article
Chemistry, Physical
Belma Gjergjizi Nallbani, Memet Vezir Kahraman, Isa Degirmenci
Summary: Computational approaches were used to elucidate radical-mediated thiol-epoxy reactions and solve the overlap problem in thiol-ene/thiol-epoxy systems. The study evaluated nine epoxy model molecules to mimic industrial epoxy molecules and proposed a thiol-ene-like mechanism for radical-mediated thiol-epoxide reactions. The findings highlighted the importance of temperature control and the use of specific thiols to avoid curing step overlap.
JOURNAL OF PHYSICAL CHEMISTRY A
(2023)
Article
Chemistry, Multidisciplinary
Kaiqing Ma, He Yang, Tianruo Shen, Yongkang Yue, Lingling Zhao, Xiaogang Liu, Fangjun Huo, Caixia Yin
Summary: Researchers developed a new probe CM-Mit for accurate detection of thiols in mitochondria. The probe has fast response and protein labeling ability, preventing diffusion out of the mitochondria, and can be used for drug screening and studying ferroptosis.
Article
Nanoscience & Nanotechnology
Kezi Cheng, Alex Chortos, Jennifer A. Lewis, David R. Clarke
Summary: Covalent adaptive networks combine the advantages of cross-linked elastomers and dynamic bonding in a single system. A simple one-pot method to prepare thiol-ene elastomers that exhibit reversible photoinduced switching from an elastomeric gel to fluid state is demonstrated. This behavior can be generalized to different composition and chemistries and allows for self-healing, remolding, and reversible adhesion.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Polymer Science
Hong Gao, Yingchun Sun, Miaomiao Wang, Bo Wu, Guoqiang Han, Ling Jin, Kui Zhang, Youyi Xia
Summary: This article introduces a novel acrylate-based elastomer with exchangeable disulfide crosslinks for self-healing and reprocessing capabilities. The use of a base system as a catalyst proves to be highly efficient, enabling the material to quickly restore over 90% of its mechanical properties within 5 hours, showing promising application potential as recoverable adhesives.
Article
Polymer Science
Baoliang Wang, Lili Xing, Tieling Xing, Guoqiang Chen
Summary: A superhydrophobic fabric was synthesized by modifying the fiber's surface with dopamine-containing hydroxyl functional groups. This fabric has a compact and regular micro-nano rough structure based on POSS and mercaptans, providing stable fastness and durability, as well as high resistance to various environmental factors. It can be used for self-cleaning, oil-water separation, and has wide applications in the coating industry.
Article
Chemistry, Physical
Wenjia Huang, Yong Li, Maoshuang Chen, Jinlin Chen, Jun Liu, Shuangfei Xiang, Feiya Fu, Xiangdong Liu, Ni Li
Summary: This study presents a new method for preparing antibacterial cotton fabrics by incorporating eugenol and L-Cysteine onto cotton fibers. The modified fabrics show high bacteriostatic reduction rates against Escherichia coli and Staphylococcus aureus and exhibit excellent antibacterial durability.
APPLIED SURFACE SCIENCE
(2024)
Article
Multidisciplinary Sciences
Yuwen Wang, Patrick Czabala, Monika Raj
Summary: In this study, the authors report a one-pot multicomponent Furan-Thiol-Amine reaction inspired by enzymes, which can generate stable pyrrole heterocycles independent of diverse functionalities. The reaction has wide applicability, including selective and irreversible labeling of peptides, synthesis of macrocyclic and stapled peptides, selective modification of different proteins with varying payloads, and labeling of lysine and cysteine in a complex human proteome.
NATURE COMMUNICATIONS
(2023)
Article
Polymer Science
Michael Zanon, Laura Montalvillo-Jimenez, Paula Bosch, Raquel Cue-Lopez, Enrique Martinez-Campos, Marco Sangermano, Annalisa Chiappone
Summary: Surgical interventions play a crucial role in modern medicine, but the rehabilitation process is essential for patients with post-treatment consequences. This study proposes a sodium alginate hydrogel, synthesized using a thiol-yne reaction, which demonstrates good self-standing properties and desirable mechanical, rheological, and topographical behavior. The hydrogels are also cytocompatible and have the potential to be used in tissue engineering and regenerative medicine.
Article
Chemistry, Applied
Youguo Qi, Ji Fan, Yuqi Chang, Yanjie Li, Bingwei Bao, Bingfei Yan, Hongqi Li, Peihong Cong
Summary: A novel smart photochromic fabric was successfully manufactured in this study, with dynamic optical control achieved through thiol-ene click chemistry principle. The fabric exhibits fast light response speed and good fine images under UV irradiation, and retains photochromic properties and reusability after multiple reversible color-changing cycles.
Article
Cell Biology
Kuanxing Gao, Man Cheng, Xinxin Zuo, Jinzhong Lin, Kurt Hoogewijs, Michael P. Murphy, Xiang-Dong Fu, Xiaorong Zhang
Summary: RNA interference (RNAi) is a gene-silencing pathway in most eukaryotic cells to protect the genome from retrotransposition. Recent studies show that transfected siRNAs can enter mitochondria and function there to specifically silence targeted mitochondrial transcripts, providing a new tool to study mitochondrial biology.
Article
Nutrition & Dietetics
Sih Min Tan, Runa S. J. Lindblom, Mark Ziemann, Adrienne Laskowski, Cesare Granata, Matthew Snelson, Vicki Thallas-Bonke, Assam El-Osta, Carlos D. Baeza-Garza, Stuart T. Caldwell, Richard C. Hartley, Thomas Krieg, Mark E. Cooper, Michael P. Murphy, Melinda T. Coughlan
Summary: The study investigated the use of MitoGamide to target methylglyoxal within the mitochondria for treating diabetic kidney disease (DKD), but found that it did not substantially improve the renal phenotype in an experimental model of diabetes. The findings suggest that targeting methylglyoxal within the mitochondria using MitoGamide may not be a valid therapeutic approach for DKD, and that other mitochondrial targets or processes upstream should be the focus of therapy.
Article
Cell Biology
Helmut Sies, Vsevolod V. Belousov, Navdeep S. Chandel, Michael J. Davies, Dean P. Jones, Giovanni E. Mann, Michael P. Murphy, Masayuki Yamamoto, Christine Winterbourn
Summary: This article discusses the key issues associated with identifying the physiological roles of oxidants, with a focus on H2O2 and O-2(.-). The article emphasizes the need for more precise measurement of oxidants and specific identification of their signaling targets. It also highlights the importance of inter-organellar communication and the contribution of environmental exposures. Development of tools for real-time detection and quantification of individual oxidants is crucial for achieving these goals.
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2022)
Article
Clinical Neurology
Tessa Kliest, Ruben P. A. Van Eijk, Ammar Al-Chalabi, Alberto Albanese, Peter M. Andersen, Maria Del Mar Amador, Geir BrAthen, Veronique Brunaud-Danel, Lev Brylev, William Camu, Mamede De Carvalho, Cristina Cereda, Hakan Cetin, Delia Chaverri, Adriano Chio, Philippe Corcia, Philippe Couratier, Fabiola De Marchi, Claude Desnuelle, Michael A. Van Es, JesUs Esteban, Massimiliano Filosto, Alberto GarcIa Redondo, Julian Grosskreutz, Clemens O. Hanemann, Trygve Holmoy, Helle Hoyer, Caroline Ingre, Blaz Koritnik, Magdalena Kuzma-Kozakiewicz, Thomas Lambert, Peter N. Leigh, Christian Lunetta, Jessica Mandrioli, Christopher J. Mcdermott, Thomas Meyer, Jesus S. Mora, Susanne Petri, MOnica Povedano, Evy Reviers, Nilo Riva, Kit C. B. Roes, Miguel A. Rubio, FranCois Salachas, Stayko Sarafov, Gianni SorarU, Zorica Stevic, Kirsten Svenstrup, Anette Torvin Moller, Martin R. Turner, Philip Van Damme, Lucie A. G. Van Leeuwen, Luis Varona, Juan F. VAzquez Costa, Markus Weber, Orla Hardiman, Leonard H. Van den Berg
Summary: In Europe, conducting clinical studies on the effect of therapy in children with ALS is not feasible. Pediatric ALS has a very low prevalence and recruiting pediatric patients may involve lengthy periods, high costs, ethical/legal implications, challenges in trial design, and limited information.
AMYOTROPHIC LATERAL SCLEROSIS AND FRONTOTEMPORAL DEGENERATION
(2022)
Review
Biochemistry & Molecular Biology
Michael P. Murphy, Edward T. Chouchani
Summary: Succinate, as a signaling modulator, plays multiple roles in biology due to its ability to reflect the redox state of the CoQ pool. Its equilibrium with the CoQ pool enables the communication of the mitochondrial status to the rest of the cell and other cells, explaining the emerging roles of succinate in biology.
NATURE CHEMICAL BIOLOGY
(2022)
Article
Oncology
Tania Capeloa, Joanna Krzystyniak, Donatienne D'Hose, Amanda Canas Rodriguez, Valery L. Payen, Luca X. Zampieri, Justine A. Van de Velde, Zohra Benyahia, Erica Pranzini, Thibaut Vazeille, Maude Fransolet, Caroline Bouzin, Davide Brusa, Carine Michiels, Bernard Gallez, Michael P. Murphy, Paolo E. Porporato, Pierre Sonveaux
Summary: This study investigates the potential of the mitochondria-targeted antioxidant MitoQ in inhibiting breast cancer metastasis. Using human breast cancer cells as models, the researchers demonstrate that MitoQ can inhibit cancer cell migration, invasion, clonogenicity, sphere formation, and spheroid stability. The findings of this study are important for future clinical research and evaluation of MitoQ for the prevention of breast cancer metastasis.
Article
Oncology
Tania Capeloa, Joanna Krzystyniak, Amanda Canas Rodriguez, Valery L. Payen, Luca X. Zampieri, Erica Pranzini, Francoise Derouane, Thibaut Vazeille, Caroline Bouzin, Francois P. Duhoux, Michael P. Murphy, Paolo E. Porporato, Pierre Sonveaux
Summary: Entry into the metastatic phase is devastating for cancer patients. Clinical studies have found that the mitochondria-targeted antioxidant MitoQ can prevent breast cancer metastasis and recurrence.
Article
Multidisciplinary Sciences
Francisco J. Roca, Laura J. Whitworth, Hiran A. Prag, Michael P. Murphy, Lalita Ramakrishnan
Summary: Excess TNF in mycobacterium-infected macrophages increases mitochondrial reactive oxygen species (mROS), causing susceptibility to tuberculosis. The study identifies the mechanism of TNF-induced mROS through reverse electron transport. The use of the drug metformin prevents TNF-induced mROS and necrosis in tuberculosis.
Article
Biochemistry & Molecular Biology
Fay M. Allen, Ana S. H. Costa, Anja Gruszczyk, Georgina R. Bates, Hiran A. Prag, Efterpi Nikitopoulou, Carlo Viscomi, Christian Frezza, Andrew M. James, Michael P. Murphy
Summary: The study developed a rapid fractionation procedure to stabilize the distribution of metabolites between mitochondria and the cytosol. The procedure revealed the compartmentation of mitochondrial metabolites in vivo and allows for the assessment of metabolite distribution between the cytosol and mitochondria in various situations.
Article
Oncology
Tania Capeloa, Justine A. Van de Velde, Donatienne D'Hose, Sara G. Lipari, Francoise Derouane, Loic Hamelin, Marie Bedin, Thibaut Vazeille, Francois P. Duhoux, Michael P. Murphy, Paolo E. Porporato, Bernard Gallez, Pierre Sonveaux
Summary: This study suggests that inhibiting mitochondrial redox signaling could be a potential therapeutic option to prevent metastatic progression of pancreatic cancer. The mitochondria-targeted antioxidant, MitoQ, selectively represses the respiration of mesenchymal pancreatic cancer cells and reduces the production of harmful substances. Additionally, MitoQ inhibits cancer cell migration, invasion, and the expression of stem cell markers. Combination treatments with chemotherapy are also feasible.
Article
Multidisciplinary Sciences
Gabriel Sturm, Anna S. Monzel, Kalpita R. Karan, Jeremy Michelson, Sarah A. Ware, Andres Cardenas, Jue Lin, Celine Bris, Balaji Santhanam, Michael P. Murphy, Morgan E. Levine, Steve Horvath, Daniel W. Belsky, Shuang Wang, Vincent Procaccio, Brett A. Kaufman, Michio Hirano, Martin Picard
Summary: This study reports a multi-omics longitudinal dataset for analyzing biological changes during aging. The dataset includes various measurements of cultured primary human fibroblasts sourced from healthy donors and individuals with mitochondrial disease. These measurements include cytological, bioenergetic, DNA methylation, gene expression, and other data. The dataset helps bridge the gap between mechanistic processes and descriptive features of aging, such as epigenetic age clocks.
Article
Biochemistry & Molecular Biology
Shinpei Uno, Alexander H. Harkiss, Roy Chowdhury, Stuart T. Caldwell, Tracy A. Prime, Andrew M. James, Brendan Gallagher, Julien Prudent, Richard C. Hartley, Michael P. Murphy
Summary: The targeting of bioactive molecules and probes to mitochondria can be achieved by coupling to the lipophilic triphenyl phosphonium (TPP) cation, which accumulates within mitochondria in response to the mitochondrial membrane potential. In this study, we investigated the effects of using a polyethylene glycol (PEG) linker instead of a simple alkane linker to enhance the water solubility of mitochondria-targeted compounds. We found that the use of PEG greatly improved the uptake of compounds by mitochondria and reduced adsorption to the mitochondrial inner membrane.
Letter
Multidisciplinary Sciences
Valerie B. O'Donnell, Nils H. Schebb, Ginger L. Milne, Michael P. Murphy, Christopher P. Thomas, Dieter Steinhilber, Stacy L. Gelhaus, Hartmut Kuehn, Michael H. Gelb, Per-Johan Jakobsson, Ian A. Blair, Robert C. Murphy, Bruce A. Freeman, Alan R. Brash, Garret A. Fitzgerald
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Andrew M. James, Abigail A. I. Norman, Jack W. Houghton, Hiran A. Prag, Angela Logan, Robin Antrobus, Richard C. Hartley, Michael P. Murphy
Summary: A new mass spectrometry probe, CysTPP, has been developed to sensitively detect major acyl-CoAs in rat tissues, revealing the potential importance of longer-chain fatty acyl-CoAs in modifying protein residues non-enzymatically.
CELL CHEMICAL BIOLOGY
(2022)
Article
Endocrinology & Metabolism
Michael P. Murphy, Hulya Bayir, Vsevolod Belousov, Christopher J. Chang, Kelvin J. A. Davies, Michael J. Davies, Tobias P. Dick, Toren Finkel, Henry J. Forman, Yvonne Janssen-Heininger, David Gems, Valerian E. Kagan, Balaraman Kalyanaraman, Nils-Goran Larsson, Ginger L. Milne, Thomas Nystrom, Henrik E. Poulsen, Rafael Radi, Holly Van Remmen, Paul T. Schumacker, Paul J. Thornalley, Shinya Toyokuni, Christine C. Winterbourn, Huiyong Yin, Barry Halliwell
Summary: This article discusses the important roles of reactive oxygen species (ROS) in health and disease, and proposes guidelines and best practices for the nomenclature and assessment of ROS, oxidative reactions, and oxidative damage in cells, tissues, and in vivo.
Correction
Biochemistry & Molecular Biology
Siew Chin Chan, Chih-Wei Tung, Chia-Wei Lin, Yun-Shiuan Tung, Po-Min Wu, Pei-Hsun Cheng, Chuan-Mu Chen, Shang-Hsun Yang
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Suyuan Liu, Meiling Tan, Jiangxue Cai, Chenxuan Li, Miaoxin Yang, Xiaoxiao Sun, Bin He
Summary: This study reveals that the antibiotic doxycycline effectively inhibits NLRP3 inflammasome activation by targeting mitochondrial translation and mtDNA synthesis, offering potential for the treatment of NLRP3-related diseases.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Hao Liu, Nana Li, Ge Kuang, Xia Gong, Ting Wang, Jun Hu, Hui Du, Minxuan Zhong, Jiashi Guo, Yao Xie, Yang Xiang, Shengwang Wu, Yiling Yuan, Xinru Yin, Jingyuan Wan, Ke Li
Summary: Protectin D1 (PTD1) improves hepatic steatosis, inflammation and fibrosis in a NASH mouse model by inhibiting the activation of TLR4 downstream signaling pathway, possibly through upregulation of IRAK-M expression, suggesting a potential new treatment for NASH.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)