Thiol redox proteomics: Characterization of thiol‐based post‐translational modifications

Commonly Used Alkylating Agents Limit Persulfide Detection by Converting Protein Persulfides into Thioethers

(2022) Danny Schilling et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A deep redox proteome profiling workflow and its application to skeletal muscle of a Duchenne Muscular Dystrophy model

(2022) Nicholas J. Day et al.   FREE RADICAL BIOLOGY AND MEDICINE

In Search of a Universal Method: A Comparative Survey of Bottom-Up Proteomics Sample Preparation Methods

(2022) Gina Varnavides et al.   JOURNAL OF PROTEOME RESEARCH

Regulation of hyperoxia-induced neonatal lung injury via post-translational cysteine redox modifications

(2022) Tong Zhang et al.   Redox Biology

SP3‐FAIMS chemoproteomics for high coverage profiling of the human cysteinome

(2021) Tianyang Yan et al.   CHEMBIOCHEM

Redox proteomics reveals an interdependence of redox modification and location of adhesome proteins in NGF-treated PC12 cells

(2021) Juliane Meißner et al.   FREE RADICAL BIOLOGY AND MEDICINE

Redox changes in obesity, metabolic syndrome, and diabetes

(2021) Bato Korac et al.   Redox Biology

Pinpointing cysteine oxidation sites by high-resolution proteomics reveals a mechanism of redox-dependent inhibition of human STING

(2021) Natalia Zamorano Cuervo et al.   Science Signaling

Stoichiometric Thiol Redox Proteomics for Quantifying Cellular Responses to Perturbations

(2021) Nicholas J. Day et al.   Antioxidants

Fluorescent Probes and Mass Spectrometry-Based Methods to Quantify Thiols in Biological Systems

(2021) Lingfei Wang et al.   ANTIOXIDANTS & REDOX SIGNALING

Cancer‐induced muscle atrophy is determined by intrinsic muscle oxidative capacity

(2021) Christiano R. R. Alves et al.   FASEB JOURNAL

Organic mercury solid phase chemoselective capture for proteomic identification of S-nitrosated proteins and peptides

(2021) Paschalis-Thomas Doulias et al.   NITRIC OXIDE-BIOLOGY AND CHEMISTRY

AlphaFold Protein Structure Database: massively expanding the structural coverage of protein-sequence space with high-accuracy models

(2021) Mihaly Varadi et al.   NUCLEIC ACIDS RESEARCH

Mass spectrometry-based direct detection of multiple types of protein thiol modifications in pancreatic beta cells under endoplasmic reticulum stress

(2021) Xiaolu Li et al.   Redox Biology

A Quantitative Tissue-Specific Landscape of Protein Redox Regulation during Aging

(2020) Haopeng Xiao et al.   CELL

Discovery of a redox thiol switch: implications for cellular energy metabolism

(2020) XingHuang Gao et al.   MOLECULAR & CELLULAR PROTEOMICS

Automated sample preparation with SP 3 for low‐input clinical proteomics

(2020) Torsten Müller et al.   Molecular Systems Biology

Spatial and temporal alterations in protein structure by EGF regulate cryptic cysteine oxidation

(2020) Jessica B. Behring et al.   Science Signaling

High-Field Asymmetric Waveform Ion Mobility Spectrometry and Native Mass Spectrometry: Analysis of Intact Protein Assemblies and Protein Complexes

(2020) Oliver J. Hale et al.   ANALYTICAL CHEMISTRY

Increased Protein S-Glutathionylation in Leber’s Hereditary Optic Neuropathy (LHON)

(2020) Lei Zhou et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

The Proteomic Landscape of Cysteine Oxidation That Underpins Retinoic Acid-Induced Neuronal Differentiation

(2020) Katrin Brenig et al.   JOURNAL OF PROTEOME RESEARCH

Measuring Reactive Sulfur Species and Thiol Oxidation States: Challenges and Cautions in Relation to Alkylation-Based Protocols

(2020) Péter Nagy et al.   ANTIOXIDANTS & REDOX SIGNALING

A clickable probe for versatile characterization of S-nitrosothiols

(2020) Jenna L. Clements et al.   Redox Biology

Protein thiol oxidation in the rat lung following e-cigarette exposure

(2020) Juan Wang et al.   Redox Biology

Stochiometric quantification of the thiol redox proteome of macrophages reveals subcellular compartmentalization and susceptibility to oxidative perturbations

(2020) Jicheng Duan et al.   Redox Biology

Large-scale analysis of redox-sensitive conditionally disordered protein regions reveals their widespread nature and key roles in high-level eukaryotic processes

(2019) Gábor Erdős et al.   PROTEOMICS

Isotopic tagging of oxidized and reduced cysteines (iTORC) for detecting and quantifying sulfenic acids, disulfides, and free thiols in cells

(2019) Matthew E. Albertolle et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Oxidative Stress: A Key Modulator in Neurodegenerative Diseases

(2019) Anju Singh et al.   MOLECULES

oxSWATH: An integrative method for a comprehensive redox-centered analysis combined with a generic differential proteomics screening

(2019) Sandra I. Anjo et al.   Redox Biology

Irreversible oxidative post-translational modifications in heart disease

(2019) Tamara Tomin et al.   Expert Review of Proteomics

Direct Proteomic Mapping of Cysteine Persulfidation

(2019) Ling Fu et al.   ANTIOXIDANTS & REDOX SIGNALING

Caution for the routine use of phenol red – It is more than just a pH indicator

(2019) Andrew Morgan et al.   CHEMICO-BIOLOGICAL INTERACTIONS

Activity-Based Sensing for Site-Specific Proteomic Analysis of Cysteine Oxidation

(2019) Yunlong Shi et al.   ACCOUNTS OF CHEMICAL RESEARCH

Selective Persulfide Detection Reveals Evolutionarily Conserved Antiaging Effects of S-Sulfhydration

(2019) Jasmina Zivanovic et al.   Cell Metabolism

Speciation of Reactive Sulfur Species and their Reactions with Alkylating Agents: Do we have any clue about what is present inside the cell?

(2018) Virág Bogdándi et al.   BRITISH JOURNAL OF PHARMACOLOGY

ROS and RNS signalling: adaptive redox switches through oxidative/nitrosative protein modifications

(2018) N. T. Moldogazieva et al.   FREE RADICAL RESEARCH

Reactive Metabolite-induced Protein Glutathionylation: a Potentially Novel Mechanism Underlying Acetaminophen Hepatotoxicity

(2018) Chun Yip CHAN et al.   MOLECULAR & CELLULAR PROTEOMICS

Fatiguing contractions increase protein S-glutathionylation occupancy in mouse skeletal muscle

(2018) Philip A. Kramer et al.   Redox Biology

Quantifying changes in the bacterial thiol redox proteome during host-pathogen interaction

(2018) Kaibo Xie et al.   Redox Biology

Identification and quantification of protein S -nitrosation by nitrite in the mouse heart during ischemia

(2017) Edward T. Chouchani et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Diverse Redoxome Reactivity Profiles of Carbon Nucleophiles

(2017) Vinayak Gupta et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Quantitative proteomic characterization of redox-dependent post-translational modifications on protein cysteines

(2017) Jicheng Duan et al.   Molecular BioSystems

Isotopically-Labeled Iodoacetamide-Alkyne Probes for Quantitative Cysteine-Reactivity Profiling

(2017) Masahiro Abo et al.   MOLECULAR PHARMACEUTICS

Architecture of the human interactome defines protein communities and disease networks

(2017) Edward L. Huttlin et al.   NATURE

Top-down characterization of endogenous protein complexes with native proteomics

(2017) Owen S Skinner et al.   Nature Chemical Biology

Harnessing Redox Cross-Reactivity To Profile Distinct Cysteine Modifications

(2016) Jaimeen D. Majmudar et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Simultaneous Enrichment of Cysteine-containing Peptides and Phosphopeptides Using a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in Combination with titanium dioxide (TiO2) Chromatography

(2016) Honggang Huang et al.   MOLECULAR & CELLULAR PROTEOMICS

S-nitrosation of proteins relevant to Alzheimer’s disease during early stages of neurodegeneration

(2016) Uthpala Seneviratne et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Profiling the reactivity of cyclic C-nucleophiles towards electrophilic sulfur in cysteine sulfenic acid

(2016) Vinayak Gupta et al.   Chemical Science

Proteomic Identification of Oxidized Proteins in Entamoeba histolytica by Resin-Assisted Capture: Insights into the Role of Arginase in Resistance to Oxidative Stress

(2016) Preeti Shahi et al.   PLoS Neglected Tropical Diseases

A novel persulfide detection method reveals protein persulfide- and polysulfide-reducing functions of thioredoxin and glutathione systems

(2016) E. Doka et al.   Science Advances

Quantitative Persulfide Site Identification (qPerS-SID) Reveals Protein Targets of H2S Releasing Donors in Mammalian Cells

(2016) Sebastian Longen et al.   Scientific Reports

A Chemical Approach for the Detection of Protein Sulfinylation

(2015) Mauro Lo Conte et al.   ACS Chemical Biology

Quantitative Profiling of Protein S-Glutathionylation Reveals Redox-Dependent Regulation of Macrophage Function during Nanoparticle-Induced Oxidative Stress

(2015) Jicheng Duan et al.   ACS Nano

The Expanding Landscape of the Thiol Redox Proteome

(2015) Jing Yang et al.   MOLECULAR & CELLULAR PROTEOMICS

Regulation of brain glutamate metabolism by nitric oxide and S-nitrosylation

(2015) Karthik Raju et al.   Science Signaling

DisGeNET: a discovery platform for the dynamical exploration of human diseases and their genes

(2015) J. Pinero et al.   Database-The Journal of Biological Databases and Curation

Quantitative H2S-mediated protein sulfhydration reveals metabolic reprogramming during the integrated stress response

(2015) Xing-Huang Gao et al.   eLife

Regulation of brain glutamate metabolism by nitric oxide and S-nitrosylation

(2015) Karthik Raju et al.   Science Signaling

Thiol-based redox switches

(2014) Bastian Groitl et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

A reductive ligation based fluorescent probe for S-nitrosothiols

(2014) Di Zhang et al.   CHEMICAL COMMUNICATIONS

Proteomic Quantification and Site-Mapping of S-Nitrosylated Proteins Using Isobaric iodoTMT Reagents

(2014) Zhe Qu et al.   JOURNAL OF PROTEOME RESEARCH

Proteome-wide Light/Dark Modulation of Thiol Oxidation in Cyanobacteria Revealed by Quantitative Site-specific Redox Proteomics

(2014) Jia Guo et al.   MOLECULAR & CELLULAR PROTEOMICS

Site-specific mapping and quantification of protein S-sulphenylation in cells

(2014) Jing Yang et al.   Nature Communications

The PI3K/AKT/mTOR pathway in breast cancer: targets, trials and biomarkers

(2014) Elisavet Paplomata et al.   Therapeutic Advances in Medical Oncology

Detection of Protein S-Sulfhydration by a Tag-Switch Technique

(2013) Dehui Zhang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Proteomic identification and quantification of S-glutathionylation in mouse macrophages using resin-assisted enrichment and isobaric labeling

(2013) Dian Su et al.   FREE RADICAL BIOLOGY AND MEDICINE

The Redox Biochemistry of Protein Sulfenylation and Sulfinylation

(2013) Mauro Lo Conte et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Direct and Nitroxyl (HNO)-Mediated Reactions of Acyloxy Nitroso Compounds with the Thiol-Containing Proteins Glyceraldehyde 3-Phosphate Dehydrogenase and Alkyl Hydroperoxide Reductase Subunit C

(2013) Susan Mitroka et al.   JOURNAL OF MEDICINAL CHEMISTRY

Measuring and Managing Ratio Compression for Accurate iTRAQ/TMT Quantification

(2013) Mikhail M. Savitski et al.   JOURNAL OF PROTEOME RESEARCH

Proteomic approaches to evaluate proteins-nitrosylation in disease

(2013) Laura M. López-Sánchez et al.   MASS SPECTROMETRY REVIEWS

Resin-assisted enrichment of thiols as a general strategy for proteomic profiling of cysteine-based reversible modifications

(2013) Jia Guo et al.   Nature Protocols

Time line of redox events in aging postmitotic cells

(2013) Nicolas Brandes et al.   eLife

Quantitative site-specific reactivity profiling of S-nitrosylation in mouse skeletal muscle using cysteinyl peptide enrichment coupled with mass spectrometry

(2012) Dian Su et al.   FREE RADICAL BIOLOGY AND MEDICINE

Mass spectrometry-based identification of S-nitrosocysteine in vivo using organic mercury assisted enrichment

(2012) Paschalis-Thomas Doulias et al.   METHODS

Using Quantitative Redox Proteomics to Dissect the Yeast Redoxome

(2011) Nicolas Brandes et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Signal transduction by reactive oxygen species

(2011) Toren Finkel   JOURNAL OF CELL BIOLOGY

Regulatory Control or Oxidative Damage? Proteomic Approaches to Interrogate the Role of Cysteine Oxidation Status in Biological Processes

(2011) Jason M. Held et al.   MOLECULAR & CELLULAR PROTEOMICS

Identification and Quantification of S-Nitrosylation by Cysteine Reactive Tandem Mass Tag Switch Assay

(2011) Christopher I. Murray et al.   MOLECULAR & CELLULAR PROTEOMICS

Identification of protein nitrosothiols using phosphine-mediated selective reduction

(2011) Sheng Li et al.   NITRIC OXIDE-BIOLOGY AND CHEMISTRY

H2S-Induced Sulfhydration of the Phosphatase PTP1B and Its Role in the Endoplasmic Reticulum Stress Response

(2011) N. Krishnan et al.   Science Signaling

Signaling Functions of Reactive Oxygen Species

(2010) Henry Jay Forman et al.   BIOCHEMISTRY

Quantitative reactivity profiling predicts functional cysteines in proteomes

(2010) Eranthie Weerapana et al.   NATURE

Structural profiling of endogenous S-nitrosocysteine residues reveals unique features that accommodate diverse mechanisms for protein S-nitrosylation

(2010) P.-T. Doulias et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Proteomic analysis of S-nitrosylation and denitrosylation by resin-assisted capture

(2009) Michael T Forrester et al.   NATURE BIOTECHNOLOGY

Quantifying the global cellular thiol-disulfide status

(2009) R. E. Hansen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Fast Reductive Ligation ofS-Nitrosothiols

(2008) Hua Wang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Quantifying changes in the thiol redox proteome upon oxidative stress in vivo

(2008) L. I. Leichert et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA