Core-shell poly(dopamine) magnetic nanoparticles for the extraction of estrogenic mycotoxins from milk and yogurt prior to LC-MS analysis

In this work, core-shell poly(dopamine) magnetic nanoparticles synthesized in our laboratory have been applied as dispersive solid-phase extraction (dSPE) sorbent for the extraction of a group of six mycotoxins of interest including zearalenone, alpha-zearalanol, beta-zearalanol, alpha-zearalenol, beta-zearalenol and zearalanone, from complex matrices such as milk (whole and skimmed cow milk and semi-skimmed goat milk) and yogurt (an unsweetened natural yogurt) prior to their LC-MS analysis. 17 beta-estradiol-D-5 was used as internal standard. The procedure includes a deproteinization step prior to the extraction procedure. Matrix-matched calibration and a recovery study were carried out in the selected matrices, providing good linearity, relative recovery values in the range 70-120% with RSDs lower than 16% and LODs between 0.21 and 4.77 mu g/L for milk samples and between 0.29 and 4.54 mu g/kg for yogurt samples. (C) 2016 Elsevier Ltd. All rights reserved.