Article
Biochemistry & Molecular Biology
Juan Salazar, Daniela Pirela, Manuel Nava, Ana Castro, Lisse Angarita, Heliana Parra, Samuel Duran-Aguero, Diana Marcela Rojas-Gomez, Nestor Galban, Roberto Anez, Maricarmen Chacin, Andrea Diaz, Nelson Villasmil, Juan Bautista De Sanctis, Valmore Bermudez
Summary: Cardiovascular disease (CVD) is a global public health issue with high morbidity, mortality, and economic impact. Specialized proresolving lipid mediators (SPMs), derived from fatty acids, have shown promise in resolving inflammation associated with the pathogenesis of various diseases, particularly CVD. Preclinical and clinical studies have demonstrated the therapeutic impact of SPMs in CVD, suggesting their potential as a new therapeutic alternative. Further large-scale clinical trials are needed to determine their effects in humans.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Joan Claria, Roger Flores-Costa, Marta Duran-Guell, Cristina Lopez-Vicario
Summary: Inflammation is a key feature of liver diseases, presenting from mild to intense stages, impacting organ function and failure. This review focuses on understanding the inflammatory process in non-alcoholic steatohepatitis and acute-on-chronic liver failure, emphasizing the importance of specialized pro-resolving mediators in regulating inflammation resolution.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2021)
Review
Biochemistry & Molecular Biology
Shayna Sandhaus, Andrew G. Swick
Summary: Specialized proresolving mediators (SPMs) are endogenous lipid metabolites that promote the resolution of inflammation. While not yet studied in the context of the novel Coronavirus, SPMs have shown potential in combating infections and inflammatory lung conditions.
Article
Endocrinology & Metabolism
Ernesto Pena Calderin, Jing-Juan Zheng, Nolan L. Boyd, Lindsey McNally, Timothy N. Audam, Pawel Lorkiewicz, Bradford G. Hill, Jason Hellmann
Summary: This study found that physical activity could enhance the production of proresolving lipid mediators and increase macrophage mitochondrial metabolism, which play a role in anti-inflammatory effects and reducing the risk of cardiovascular diseases.
MOLECULAR METABOLISM
(2022)
Article
Cell Biology
Shahd AlZahrani, Zakia Shinwari, Ameera Gaafar, Ayodele Alaiya, Ahmed Al-Kahtani
Summary: The interconnection between tissue inflammation and regeneration has been confirmed through the regulation of defense and repair mechanisms in diseased dental tissue. This study focuses on the effects of specific pro-resolving mediators (SPMs) on inflamed human bone marrow-derived mesenchymal stem cells (hBMMSCs). The results show that the combination of Resolvin E1 (RvE1) and Maresin 1 (MaR1) effectively reduces inflammation in hBMMSCs.
Review
Biochemistry & Molecular Biology
Mariaenrica Tine, Ylenia Padrin, Matteo Bonato, Umberto Semenzato, Erica Bazzan, Maria Conti, Marina Saetta, Graziella Turato, Simonetta Baraldo
Summary: Asthma is a common chronic respiratory disorder with a growing incidence worldwide. Personalized approaches to treating asthma have emerged based on improved understanding of asthma pathogenesis. Extracellular vesicles (EVs) have been recognized as key mediators of cell-to-cell interplay in asthma, with studies showing a pro-inflammatory and pro-remodeling role of EVs. However, standardization of EV isolation and careful patient selection are needed for reliable results and further application as biomarkers in asthma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Nan Chiang, Xavier de la Rosa, Stephania Libreros, Hui Pan, Jonathan M. Dreyfuss, Charles N. Serhan
Summary: The study found that cysteinyl-specialized proresolving mediators activate key pathways in planaria regeneration, linking inflammation resolution and regeneration. The results demonstrate a crucial role for TRAF3/IL-10 in regulating mammalian phagocyte functions.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cardiac & Cardiovascular Systems
Stephanie M. Cicalese, Josiane Fernandes da Silva, Fernanda Priviero, R. Clinton Webb, Satoru Eguchi, Rita C. Tostes
Summary: Cells respond to stress by activating defense signaling pathways for survival or cell death. Arterial hypertension and aging share mechanisms that mediate chronic cell stress, potentially leading to unresolved pathologies.
CIRCULATION RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Lucia Serrano Diaz Del Campo, Raquel Rodrigues-Diez, Mercedes Salaices, Ana M. Briones, Ana B. Garcia-Redondo
Summary: Vascular remodeling is a typical feature of vascular diseases, and inflammation plays a key role in regulating this process. Specialized pro-resolving mediators (SPMs) can modulate the function of vascular cells and protect against the development of vascular remodeling in cardiovascular diseases such as atherosclerosis, aneurysms, and restenosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Dentistry, Oral Surgery & Medicine
Elisabetta Cotti, Francesca Ideo, Alessandro Pedrazzini, Giulia Bardini, Davide Musu, Alpdogan Kantarci
Summary: This study investigated the potential of specialized proresolving lipid mediators (SPMs) in the treatment of endodontic infection by analyzing scientific literature. The results showed that SPMs positively affected the development of pulpitis and apical periodontitis in animal models, suggesting their role as a promising adjunct in future endodontic treatment.
JOURNAL OF ENDODONTICS
(2021)
Review
Cell Biology
Greg Berumen Sanchez, Kaitlyn E. Bunn, Heather H. Pua, Marjan Rafat
Summary: EVs play a crucial role in intercellular communication by carrying a variety of factors that can impact recipient cells. Studying the role of EVs provides insights into the molecular mechanisms of cancer, metabolic disorders, and inflammatory lung diseases.
CELL COMMUNICATION AND SIGNALING
(2021)
Article
Biochemistry & Molecular Biology
Haoyang Zhou, Dafeng Yang, Henry S. Cheng, Michael G. McCoy, Daniel Perez-Cremades, Stefan Haemmig, Danny Wong, Lei Chen, Mark W. Feinberg
Summary: The study uncovers an important role of miR-181b in regulating vascular endothelial aging through the MAP3K3-MAPK signaling pathway, revealing potential therapeutic targets for anti-aging therapy in cardiovascular disease.
Article
Multidisciplinary Sciences
Nunzia Caporarello, Jisu Lee, Tho X. Pham, Dakota L. Jones, Jiazhen Guan, Patrick A. Link, Jeffrey A. Meridew, Grace Marden, Takashi Yamashita, Collin A. Osborne, Aditya Bhagwate, Steven K. Huang, Roberto F. Nicosia, Daniel J. Tschumperlin, Maria Trojanowska, Giovanni Ligresti
Summary: Vascular dysfunction plays a significant role in aging and chronic diseases, but its involvement in lung repair and fibrosis remains unclear. In this study, the authors identified the endothelial transcription factor ERG as a mediator of vascular repair, whose function declines in aged lungs, resulting in sustained fibrosis. These findings highlight the importance of understanding the mechanisms underlying vascular dysfunction in lung fibrosis and provide potential targets for therapeutic interventions.
NATURE COMMUNICATIONS
(2022)
Review
Immunology
Dominika Ambrozej, Anna Stelmaszczyk-Emmel, Malgorzata Czystowska-Kuzmicz, Wojciech Feleszko
Summary: Extracellular vesicles play a vital role in intracellular communication in the lung microenvironment, particularly in the pro-inflammatory responses related to asthma. Noninvasively collected extracellular vesicles can serve as a liquid biopsy to provide information on disease status, especially in pediatric patients. Further research on extracellular vesicles can help develop improved diagnostics and personalized treatments for asthma.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Sonia Kiran, Mousumi Mandal, Ahmed Rakib, Amandeep Bajwa, Udai P. Singh
Summary: This study investigates the role and mechanism of miR-10a-3p in adipose inflammation and adipogenesis using ex vivo and in vitro approaches. The findings suggest that miR-10a-3p mimic reduces the expression of inflammatory cells and cytokines, and induces the expression of FoxP3 in adipose tissue. In vitro experiments also show that miR-10a-3p mimic reduces the expression of inflammatory genes and lipid accumulation. This study provides a new opportunity for the development of miR-10a-3p as a novel therapeutic for adipose inflammation and its associated metabolic disorders.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Antonio Recchiuti, Sara Patruno, Domenico Mattoscio, Elisa Isopi, Antonella Pomilio, Alessia Lamolinara, Manuela Iezzi, Romina Pecce, Mario Romano
Summary: An excessive and unresolved inflammatory response is one of the reasons underlying severe COVID-19, and the investigation of endogenous pathways to resolve inflammation is crucial for mitigating inflammation caused by SARS-CoV-2 infection. Individuals with preexisting pathologies like cystic fibrosis exhibit some specificity in immune responses to SARS-CoV-2. Furthermore, resolvins D1 and D2 have beneficial regulatory effects in reducing SARS-CoV-2-induced inflammation.
Article
Oncology
Domenico Mattoscio, Elisa Isopi, Alessia Lamolinara, Sara Patruno, Alessandro Medda, Federica De Cecco, Susanna Chiocca, Manuela Iezzi, Mario Romano, Antonio Recchiuti
Summary: The study shows that RvD1 can reduce cancer growth by activating PMN anti-cancer activities and promoting the recruitment of anti-tumor monocytes. In HPV-positive tumor models, RvD1 can decrease the proliferation of cancer cells and lead to better clinical outcomes for patients.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Licia Totani, Concetta Amore, Antonio Piccoli, Giuseppe Dell'Elba, Angelo Di Santo, Roberto Plebani, Romina Pecce, Nicola Martelli, Alice Rossi, Serena Ranucci, Ida De Fino, Paolo Moretti, Alessandra Bragonzi, Mario Romano, Virgilio Evangelista
Summary: This study demonstrates that PDE4 inhibitors can control NETosis in CF-relevant models, showing efficacy in neutrophils from both healthy volunteers and CF patients. In a mouse model of chronic Pseudomonas aeruginosa infection, aerosol treatment with the selective PDE4 inhibitor roflumilast resulted in reduced free DNA in the BALF along with decreased citrullination of histone H3 in neutrophils in the airways.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Respiratory System
Roberto Plebani, Ratnakar Potla, Mercy Soong, Haiqing Bai, Zohreh Izadifar, Amanda Jiang, Renee N. Travis, Chaitra Belgur, Alexandre Dinis, Mark J. Cartwright, Rachelle Prantil-Baun, Pawan Jolly, Sarah E. Gilpin, Mario Romano, Donald E. Ingber
Summary: This study developed a microfluidic device using organ-on-a-chip technology to replicate the features of human cystic fibrosis (CF) lungs. The device successfully captured CF airway characteristics, including mucus accumulation, increased cilia density, and faster ciliary beating frequency. Additionally, it simulated the inflammatory response and infection levels in CF patients, as well as provided a conducive environment for the growth of Pseudomonas aeruginosa.
JOURNAL OF CYSTIC FIBROSIS
(2022)
Article
Cell Biology
Virginia Lotti, Flavia Merigo, Anna Lagni, Andrea Di Clemente, Marco Ligozzi, Paolo Bernardi, Giada Rossini, Ercole Concia, Roberto Plebani, Mario Romano, Andrea Sbarbati, Claudio Sorio, Davide Gibellini
Summary: People with cystic fibrosis may be at higher risk for severe symptoms of COVID-19, but they have a lower ability to spread SARS-CoV-2. This study found that CFTR is involved in the regulation of SARS-CoV-2 replication, providing new insights into the development of therapeutic strategies for COVID-19.
Article
Biochemistry & Molecular Biology
Domenico Mattoscio, Giulia Ferri, Claudia Miccolo, Susanna Chiocca, Mario Romano, Antonio Recchiuti
Summary: In human medicine, predicting the progression of tumor development and resolution of inflammation remains challenging. By evaluating the expression level of genes involved in the synthesis, signaling, and metabolism of D-series resolvin, a cumulative score was used to analyze clinical, cellular, and molecular features of tumors. Higher scores were associated with better clinical outcomes and enhanced anti-tumor immunity in head and neck squamous cell carcinoma patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Nutrition & Dietetics
Domitilla Mandatori, Assunta Pandolfi
Article
Chemistry, Multidisciplinary
Emira D'Amico, Tania Vanessa Pierfelice, Giovanna Iezzi, Natalia Di Pietro, Stefania Lepore, Felice Lorusso, Antonio Scarano, Assunta Pandolfi, Adriano Piattelli, Morena Petrini
Summary: Apigenin (APG), a natural flavonoid compound with anti-inflammatory and antioxidative properties, has the potential to promote proliferation and mineralization of human oral osteoblasts, indicating its possible usefulness in dentistry.
APPLIED SCIENCES-BASEL
(2022)
Article
Nutrition & Dietetics
Konstantinos Efthymakis, Giuseppina Bologna, Pasquale Simeone, Laura Pierdomenico, Giulia Catitti, Simone Vespa, Angelo Milano, Domenico De Bellis, Francesco Laterza, Assunta Pandolfi, Caterina Pipino, Michele Sallese, Marco Marchisio, Sebastiano Miscia, Matteo Neri, Paola Lanuti
Summary: Extracellular vesicles (EVs) play important roles in intercellular communication, homeostasis maintenance, and diseases. This study aimed to assess and characterize circulating EVs in celiac disease patients and patients on a gluten-free diet (GFD) using a novel flow cytometry technique. The results showed significantly increased total EV counts in both newly diagnosed celiac disease patients and patients on GFD, while endothelial-derived and epithelial-derived EV counts were significantly decreased in subjects on gluten exclusion. These findings indicate that EVs could serve as potential disease-specific biomarkers in celiac disease.
Article
Multidisciplinary Sciences
Roberto Plebani, Alessandra D'Alessandro, Paola Lanuti, Pasquale Simeone, Massimiliano Cinalli, Ilaria Righi, Alessandro Palleschi, Matteo Mucci, Marco Marchisio, Francesco Cappabianca, Marina Camera, Felice Mucilli, Mario Romano
Summary: The availability of cells isolated from healthy and diseased tissues and organs is crucial for personalized medicine. Biobanks provide a wide range of cells, but not all experimental needs are covered, especially those related to specific diseases or genotypes. Vascular endothelial cells (ECs) play a central role in various disorders, and obtaining specific EC types is essential for reliable experiments. This article illustrates simple procedures to obtain human macrovascular and microvascular endothelial cells, which can be cost-effective and independent of commercial sources.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Biochemistry & Molecular Biology
Valeria Schiavone, Tea Romasco, Nadia Di Pietrantonio, Stefania Garzoli, Carola Palmerini, Pamela Di Tomo, Caterina Pipino, Domitilla Mandatori, Rossella Fioravanti, Elena Butturini, Manuela Sabatino, Maria Pompea Antonia Baldassarre, Rino Ragno, Assunta Pandolfi, Natalia Di Pietro
Summary: Essential oils derived from aromatic plants have been found to improve the lipid and glycemic profile of patients with diabetes mellitus. This study investigated the potential anti-inflammatory effect of anise and laurel essential oils on endothelial cells isolated from females with gestational diabetes mellitus. The results showed that both essential oils significantly reduced monocyte adhesion, VCAM-1 expression, and NF-κB p65 nuclear translocation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Pharmacology & Pharmacy
Letizia Pelusi, Domitilla Mandatori, Leonardo Mastropasqua, Luca Agnifili, Marcello Allegretti, Mario Nubile, Assunta Pandolfi
Summary: Ocular drug delivery remains a challenge due to the limitations of conventional dosage forms and the anatomical complexity of the eye. Recent developments in materials and technologies have led to the creation of various ocular drug delivery systems, such as inserts, contacts, and intraocular lenses, which show promise for future clinical applications.
Review
Biochemistry & Molecular Biology
Roberto Plebani, Haiqing Bai, Longlong Si, Jing Li, Chunhe Zhang, Mario Romano
Summary: The COVID-19 pandemic caused by SARS-CoV-2 has led to millions of deaths worldwide, highlighting the need for experimental models that accurately mimic human organ responses to the infection. Three-dimensional cell cultures, such as organoids and bioengineered organ chips, are emerging as crucial tools for studying respiratory diseases and developing antiviral drugs and vaccines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Polymer Science
Tania Vanessa Pierfelice, Emira D'Amico, Morena Petrini, Assunta Pandolfi, Camillo D'Arcangelo, Natalia Di Pietro, Adriano Piattelli, Giovanna Iezzi
Summary: This study evaluated the effects of a new photodynamic protocol (ALAD-PDT) on human gingival fibroblasts (HGF) and primary human osteoblasts (HOB). The results showed that ALAD-PDT had no cytotoxic effects and promoted cell proliferation.
