Evaluating the use of PCR for diagnosing invasive aspergillosis

Practice Guidelines for the Diagnosis and Management of Aspergillosis: 2016 Update by the Infectious Diseases Society of America

(2016) Thomas F. Patterson et al.   CLINICAL INFECTIOUS DISEASES

Prospective multicentre PCR-based Aspergillus DNA screening in high-risk patients with and without primary antifungal mould prophylaxis

(2016) J. Springer et al.   CLINICAL MICROBIOLOGY AND INFECTION

PCR methodology and applications for the detection of human fungal pathogens

(2016) Matthew William McCarthy et al.   EXPERT REVIEW OF MOLECULAR DIAGNOSTICS

PCR-based detection of Aspergillus fumigatus Cyp51A mutations on bronchoalveolar lavage: a multicentre validation of the AsperGenius assay ® in 201 patients with haematological disease suspected for invasive aspergillosis

(2016) G. M. Chong et al.   JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY

Detection of Aspergillus fumigatus in Blood Samples from Critically Ill Patients in Intensive Care Units by Use of the SeptiFast Assay: TABLE 1

(2016) Joerg Steinmann et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Comparison of Performance Characteristics of Aspergillus PCR in Testing a Range of Blood-Based Samples in Accordance with International Methodological Recommendations

(2016) Jan Springer et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Molecular Diagnostic Testing for Aspergillus

(2016) Margaret V. Powers-Fletcher et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Variation in copy number of the 28S rDNA of Aspergillus fumigatus measured by droplet digital PCR and analog quantitative real-time PCR

(2016) Alexandre Alanio et al.   JOURNAL OF MICROBIOLOGICAL METHODS

Performance evaluation of multiplex PCR including Aspergillus—not so simple!: Table 1.

(2016) Alexandre Alanio et al.   MEDICAL MYCOLOGY

Galactomannan testing andAspergillusPCR in same-day bronchoalveolar lavage and blood samples for diagnosis of invasive aspergillosis

(2016) Susanne Eigl et al.   MEDICAL MYCOLOGY

AspergillusPolymerase Chain Reaction: Systematic Review of Evidence for Clinical Use in Comparison With Antigen Testing

(2015) P. Lewis White et al.   CLINICAL INFECTIOUS DISEASES

Analytical and Clinical Evaluation of the PathoNostics AsperGenius Assay for Detection of Invasive Aspergillosis and Resistance to Azole Antifungal Drugs during Testing of Serum Samples

(2015) P. Lewis White et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Aspergillus-Specific Lateral-Flow Device and Real-Time PCR Testing of Bronchoalveolar Lavage Fluid: a Combination Biomarker Approach for Clinical Diagnosis of Invasive Pulmonary Aspergillosis

(2015) Gemma L. Johnson et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Validation of a New Aspergillus Real-Time PCR Assay for Direct Detection of Aspergillus and Azole Resistance of Aspergillus fumigatus on Bronchoalveolar Lavage Fluid

(2015) Ga-Lai M. Chong et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Biomarker-based diagnostic work-up of invasive pulmonary aspergillosis in immunocompromised paediatric patients - isAspergillusPCR appropriate?

(2015) Dieter Buchheidt et al.   MYCOSES

Molecular diagnostic methods for invasive fungal disease: the horizon draws nearer?

(2015) C.L. Halliday et al.   PATHOLOGY

Considerations for Digital PCR as an Accurate Molecular Diagnostic Tool

(2014) J. F. Huggett et al.   CLINICAL CHEMISTRY

Serum Galactomannan Versus a Combination of Galactomannan and Polymerase Chain Reaction-Based Aspergillus DNA Detection for Early Therapy of Invasive Aspergillosis in High-Risk Hematological Patients: A Randomized Controlled Trial

(2014) J. M. Aguado et al.   CLINICAL INFECTIOUS DISEASES

Clinical utility of Aspergillus galactomannan and PCR in bronchoalveolar lavage fluid for the diagnosis of invasive pulmonary aspergillosis in patients with haematological malignancies

(2014) Siow-Chin Heng et al.   DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE

Improved Sensitivity for Molecular Detection of Bacterial and Candida Infections in Blood

(2014) Andrea Bacconi et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Performance of Galactomannan, Beta-D-Glucan, Aspergillus Lateral-Flow Device, Conventional Culture, and PCR Tests with Bronchoalveolar Lavage Fluid for Diagnosis of Invasive Pulmonary Aspergillosis

(2014) M. Hoenigl et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Evaluation of a Commercially Developed Semiautomated PCR-Surface-Enhanced Raman Scattering Assay for Diagnosis of Invasive Fungal Disease

(2014) P. L. White et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Incidence of Cyp51 A Key Mutations in Aspergillus fumigatus—A Study on Primary Clinical Samples of Immunocompromised Patients in the Period of 1995–2013

(2014) Birgit Spiess et al.   PLoS One

Digital PCR as a Novel Technology and Its Potential Implications for Molecular Diagnostics

(2013) J. F. Huggett et al.   CLINICAL CHEMISTRY

Utility of bronchoalveolar lavage fluid galactomannan alone or in combination with PCR for the diagnosis of invasive aspergillosis in adult hematology patients: A systematic review and meta-analysis

(2013) Siow Chin Heng et al.   CRITICAL REVIEWS IN MICROBIOLOGY

Invasive Aspergillosis: New Insights into Disease, Diagnostic and Treatment

(2013) Meinolf Karthaus et al.   CURRENT PHARMACEUTICAL DESIGN

Development and Evaluation of a Calibrator Material for Nucleic Acid-Based Assays for Diagnosing Aspergillosis

(2013) G. M. Lyon et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Multicenter Comparison of Serum and Whole-Blood Specimens for Detection of Aspergillus DNA in High-Risk Hematological Patients

(2013) J. Springer et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Aspergillus PCR-Based Investigation of Fresh Tissue and Effusion Samples in Patients with Suspected Invasive Aspergillosis Enhances Diagnostic Capabilities

(2013) M. Reinwald et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Galactomannan and PCR versus culture and histology for directing use of antifungal treatment for invasive aspergillosis in high-risk haematology patients: a randomised controlled trial

(2013) C Orla Morrissey et al.   LANCET INFECTIOUS DISEASES

Volume dependency for culture of fungi from respiratory secretions and increased sensitivity ofAspergillusquantitative PCR

(2013) Marcin G. Fraczek et al.   MYCOSES

Diagnostic Performance of an Aspergillus-Specific Nested PCR Assay in Cerebrospinal Fluid Samples of Immunocompromised Patients for Detection of Central Nervous System Aspergillosis

(2013) Mark Reinwald et al.   PLoS One

Development of Novel PCR Assays To Detect Azole Resistance-Mediating Mutations of theAspergillus fumigatus cyp51AGene in Primary Clinical Samples from Neutropenic Patients

(2012) Birgit Spiess et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Therapy with antifungals decreases the diagnostic performance of PCR for diagnosing invasive aspergillosis in bronchoalveolar lavage samples of patients with haematological malignancies

(2012) M. Reinwald et al.   JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY

Detection, Identification, and Distribution of Fungi in Bronchoalveolar Lavage Specimens by Use of Multilocus PCR Coupled with Electrospray Ionization/Mass Spectrometry

(2012) Jeong Hwan Shin et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Rapid Detection and Identification of Aspergillus from Lower Respiratory Tract Specimens by Use of a Combined Probe-High-Resolution Melting Analysis

(2012) M. Alonso et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Diagnostic Accuracy of PCR Alone Compared to Galactomannan in Bronchoalveolar Lavage Fluid for Diagnosis of Invasive Pulmonary Aspergillosis: a Systematic Review

(2012) T. Avni et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Comparison of quantitative real time PCR with Sequencing and ribosomal RNA-FISH for the identification of fungi in Formalin fixed, paraffin-embedded tissue specimens

(2011) Volker Rickerts et al.   BMC INFECTIOUS DISEASES

Detection of Aspergillus in lung and other tissue samples using the MycAssay Aspergillus real-time PCR kit

(2011) C. Lass-Flörl et al.   CANADIAN JOURNAL OF MICROBIOLOGY

Evaluation of Luminex xTAG Fungal Analyte-Specific Reagents for Rapid Identification of Clinically Relevant Fungi

(2011) N. Esther Babady et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Multiplex Blood PCR in Combination with Blood Cultures for Improvement of Microbiological Documentation of Infection in Febrile Neutropenia

(2010) F. Lamoth et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Critical Stages of Extracting DNA from Aspergillus fumigatus in Whole-Blood Specimens

(2010) P. L. White et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Aspergillus PCR: One Step Closer to Standardization

(2010) P. L. White et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Diagnosis of invasive fungal infections by a real-time panfungal PCR assay in immunocompromised pediatric patients

(2010) C Landlinger et al.   LEUKEMIA

PCR-based diagnosis of human fungal infections

(2009) Prasanna D Khot et al.   Expert Review of Anti-Infective Therapy

An update on the molecular diagnosis of invasive fungal disease

(2009) P. Lewis White et al.   FEMS MICROBIOLOGY LETTERS

Strain-Dependent Variation in 18S Ribosomal DNA Copy Numbers in Aspergillus fumigatus

(2009) M. L. Herrera et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Utility of a Commercially Available Multiplex Real-Time PCR Assay To Detect Bacterial and Fungal Pathogens in Febrile Neutropenia

(2009) M. von Lilienfeld-Toal et al.   JOURNAL OF CLINICAL MICROBIOLOGY

A prospective randomized controlled trial comparing PCR-based and empirical treatment with liposomal amphotericin B in patients after allo-SCT

(2008) H Hebart et al.   BONE MARROW TRANSPLANTATION

Diagnosis of invasive aspergillosis by galactomannan antigenemia detection using an enzyme immunoassay

(2008) L. J. Wheat et al.   EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES

Molecular diagnosis of sepsis in neutropenic patients with haematological malignancies

(2008) N. Mancini et al.   JOURNAL OF MEDICAL MICROBIOLOGY