Article
Engineering, Environmental
Yuhao Weng, Xiaoqian Chen, Huihong Chen, Huilin Yang, Zhigang Gong, Hongliang Tan
Summary: This study developed a hydrogel microreactor GHAP that generates •OH and NO sequentially for synergistic bacterial inactivation, offering a promising solution to combat bacterial infections and antibiotic resistance.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Chemistry, Analytical
Yanan Chen, Lei Jiao, Ruimin Li, Lijun Hu, Xiangkun Jia, Zhijun Zhu, Yanling Zhai, Xiaoquan Lu
Summary: In this study, a highly sensitive and stable biosensing platform for glucose detection was developed by immobilizing glucose oxidase on AuCu hydrogels, which exhibited enhanced catalytic activity and satisfactory stability. The platform enabled dual-mode detection of glucose through electrochemical and visual methods, and showed good selectivity and a wide linear range.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Myrto G. G. Bellou, Elena Gkantzou, Anastasia Skonta, Dimitrios Moschovas, Konstantinos Spyrou, Apostolos Avgeropoulos, Dimitrios Gournis, Haralambos Stamatis
Summary: This study developed immobilized enzyme microreactors using 3D printing technology for biocatalytic processes in DES-based media. The study found that DESs had negligible effects on enzyme activity and stability, and the system proved stable and reusable in the presence of DESs. The microreactor system showed enhanced substrate accessibility and high productivity compared to batch reaction systems.
Article
Biochemistry & Molecular Biology
Changjun Liu, Yanmin Song, Tianhao Hu, Shan Wang, Ke Yi, Jianjie Wang, Qing Yan, Li'an Wei, Zheyang Zhang, Huimin Li, Yutao Luo, Lei Wu, Dongyi Zhang, Er Meng
Summary: Enzyme immobilization by 3D printing can enhance ATP regeneration and operability, while reducing cost. Fused with spidroin, the chimeric enzyme ADK-RC forms self-assembling micellar nanoparticles with high activity, thermostability, pH stability, and organic solvent tolerance. The use of ADK-RC hydrogels in 3D bioprinting increases substrate affinity and usage frequency, providing an efficient strategy for maintaining enzyme activity and reducing leakage.
Article
Chemistry, Multidisciplinary
Naohiro Kameta, Wuxiao Ding
Summary: The adsorption and cross-linking of proteins on the surface of a supramolecular nanotube, followed by the removal of the supramolecular nanotube through water degradation, resulted in protein nanotubes with high thermal/chemical stabilities.
MATERIALS CHEMISTRY FRONTIERS
(2022)
Article
Chemistry, Analytical
Yolanda Alacid, Rocio Esquembre, Francisco Montilla, Maria Jose Martinez-Tome, C. Reyes Mateo
Summary: This work presents the development and characterization of fluorescent nanocomposite hydrogels using a green protocol. The hydrogels have high swelling and absorption capacity and are prepared by incorporating polyfluorenes-based nanoparticles into a three-dimensional polymeric network. The hydrogels show good storage stability and homogeneity when using the ex situ method. They have been tested as a sensing platform for the detection of alkaline phosphatase with a quick response time and low LOD, making them promising for quantifying physiological ALP levels and enzyme activity in environmental samples.
Article
Materials Science, Multidisciplinary
Christoph Horn, Doris Pospiech, Peter J. Allertz, Martin Mueller, Katrin Salchert, Rolf Hommel
Summary: Hydrogels immobilized with enzymes are synthesized using UV-initiated radical polymerization with HEMA, ITA, ECPPA, and BAAP. ITA-LAC modification enhances enzyme activity and pH-dependent swelling behavior.
ACS APPLIED POLYMER MATERIALS
(2021)
Article
Biotechnology & Applied Microbiology
Gordana Hojnik Podrepsek, Zeljko Knez, Maja Leitgeb
Summary: Immobilized enzymes have higher stability and are easier to use. This study optimized the processes of immobilizing enzymes using CLEAs and mCLEAs methods, and compared the differences between the two methods. The results showed that the activity of immobilized enzymes varied depending on the type of enzyme, and magnetic nanoparticles played an important role in stabilizing the immobilized structure.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Environmental
Kai Lin, Ao Xia, Yun Huang, Xianqing Zhu, Kaiyong Cai, Zidong Wei, Qiang Liao
Summary: In this study, a microreactor loaded with cellulase based on co-deposition of dopamine (DA) and polyethyleneimine (PEI) was proposed to efficiently hydrolyze carboxymethyl cellulose (CMC) and produce sugar. The cellulase-loaded microreactor showed better temperature adaptability and higher long-term stability than other reaction systems, resulting in higher glucose production per unit time.
CHEMICAL ENGINEERING JOURNAL
(2022)
Review
Environmental Sciences
P. R. Yaashikaa, M. Keerthana Devi, P. Senthil Kumar
Summary: This review summarizes the basic concepts of enzyme immobilization, different immobilization techniques, and carriers used for immobilization. It also provides an in-depth overview of the potential of immobilized enzymes as bioremediation agents for the degradation of pollutants and the impact of different operating parameters. Additionally, future trends and challenges for enhancing the potential of immobilized systems for large-scale industrial wastewater treatment are discussed.
Article
Chemistry, Analytical
Diogo Alexandre Siebert, Natalia Bruzamarello Caon, Michele Debiasi Alberton, Luciano Vitali, Alexandre Luis Parize, Gustavo Amadeu Micke
Summary: This study proposes a novel enzyme micro-reactor using acetylcholinesterase anchored on magnetic nanoparticles and immobilized in capillaries using chitosan and glutaraldehyde. The enzyme showed a Km value of 1.12 mmol L-1, comparable to other immobilized enzyme studies. The method was also used to evaluate IC50 for neostigmine and showed good stability with at least 25 experiments possible. The method was applied to hydroalcoholic extracts of 7 plant species, with Plinia cauliflora showing the best enzyme inhibition of 42.31±6.81% at a concentration of 100 mg L-1.
ANALYTICA CHIMICA ACTA
(2023)
Review
Biochemistry & Molecular Biology
Zhongbiao Tan, Hairong Cheng, Gang Chen, Fang Ju, Jesus Fernandez-Lucas, Jakub Zdarta, Teofil Jesionowski, Muhammad Bilal
Summary: In recent years, enzyme-based biocatalytic systems have gained increasing attention in industrial and applied research. These systems have been applied in sustainable and environmentally friendly production processes in various industries. However, the low stability of enzymes and difficulties in separation, recovery, and reusability have limited their industrial-scale manufacturing. Immobilization and co-immobilization on robust support materials have emerged as valuable strategies to overcome these challenges. By using environmentally friendly bio-polymers and nanostructured materials, multi-enzyme co-immobilization offers improved process efficiency and cost-effectiveness in biotechnological and chemical industries.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Biochemistry & Molecular Biology
F. Rafiee, M. Rezaee
Summary: Immobilized enzymes, particularly lipase on chitosan support, have attracted significant attention due to their advantages in industry and biochemistry. This review highlights the recent studies on lipase immobilization and discusses the potential solutions for current limitations.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Chemistry, Multidisciplinary
Yu Wu, Weiqing Xu, Lei Jiao, Wenling Gu, Dan Du, Liuyong Hu, Yuehe Lin, Chengzhou Zhu
Summary: Enzymes have great potential in various fields due to their high activity and specificity for catalysis under mild conditions. However, they struggle to adapt to different environments outside of their familiar biological conditions. Nanobiocatalysis, which integrates nanotechnology and enzymatic catalysis, aims to design biocatalysis based on materials science. Nano-integrated biocatalysis and bio-inspired nanocatalysis are two effective nanobiocatalytic systems that can meet different design needs, and their combination opens up even more possibilities. This review summarizes the types, constructions, and functions of nanobiocatalytic systems, analyzing the pros and cons of different strategies. Additionally, it highlights their applications in bioassay, biotherapy, and environmental remediation.
CHEMICAL SOCIETY REVIEWS
(2022)
Review
Biochemistry & Molecular Biology
Veymar G. Tacias-Pascacio, Roberto Morellon-Sterling, Daniel Castaneda-Valbuena, Angel Berenguer-Murcia, Majid Rasool Kamli, Olga Tavano, Roberto Fernandez-Lafuente
Summary: This paper reviews the immobilization of papain on different supports for the first time, with a special focus on the preparation of magnetic biocatalysts and specific problems related to the immobilization of proteases. The benefits of proper immobilization and potential artifacts are discussed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Thermodynamics
Andres Gonzalez de Castilla, Jan Philipp Bittner, Simon Mueller, Sven Jakobtorweihen, Irina Smirnova
JOURNAL OF CHEMICAL AND ENGINEERING DATA
(2020)
Article
Engineering, Environmental
X. Hu, J. Karnetzke, M. Fassbender, S. Druecker, S. Bettermann, B. Schroeter, W. Pauer, H-U Moritz, B. Fiedler, G. Luinstra, I Smirnova
CHEMICAL ENGINEERING JOURNAL
(2020)
Article
Biochemistry & Molecular Biology
Juliane Vigano, Aureliano A. D. Meirelles, Grazielle Nathia-Neves, Andressa M. Baseggio, Rosiane L. Cunha, Mario R. Marostica Junior, M. Angela A. Meireles, Pavel Gurikov, Irina Smirnova, Julian Martinez
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2020)
Article
Biochemistry & Molecular Biology
Halil Koyu, Aslihan Kazan, Ayse Nalbantsoy, Husniye Tansel Yalcin, Ozlem Yesil-Celiktas
MOLECULAR BIOLOGY REPORTS
(2020)
Review
Agricultural Engineering
Hector A. Ruiz, Marc Conrad, Shao-Ni Sun, Arturo Sanchez, George J. M. Rocha, Aloia Romani, Eulogio Castro, Ana Torres, Rosa M. Rodriguez-Jasso, Liliane P. Andrade, Irina Smirnova, Run-Cang Sun, Anne S. Meyer
BIORESOURCE TECHNOLOGY
(2020)
Article
Thermodynamics
Simon Mueller, Andres Gonzalez de Castilla, Christoph Taeschler, Andreas Klein, Irina Smirnova
FLUID PHASE EQUILIBRIA
(2020)
Editorial Material
Chemistry, Physical
Irina Smirnova, Pavel Gurikov, Lorenz Ratke
JOURNAL OF SUPERCRITICAL FLUIDS
(2020)
Article
Biochemistry & Molecular Biology
Ecem Saygili, Elif Kaya, Esra Ilhan-Ayisigi, Pelin Saglam-Metiner, Emine Alarcin, Aslihan Kazan, Ezgi Girgic, Yong-Woo Kim, Kasim Gunes, Guler Gamze Eren-Ozcan, Dilek Akakin, Jeong-Yun Sun, Ozlem Yesil-Celiktas
Summary: Functionalized PAAm-Alg hydrogels were developed as an articular-like tissue, displaying good mechanical stability and higher viscoelasticity. Experimental results demonstrated the potential therapeutic effect of these hydrogels for cartilage regeneration.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Materials Science, Paper & Wood
Baldur Schroeter, Velislava P. Yonkova, Monika Goslinska, Maike Orth, Swantje Pietsch, Pavel Gurikov, Irina Smirnova, Stefan Heinrich
Summary: The study successfully applied protective and homogeneous shellac coating layers to the surface of low-density cellulose aerogel particles using an innovative miniaturized spouted bed setup, while maintaining the aerogels' microstructure intact. Controlled release of vanillin from coated particles was achieved, demonstrating the effectiveness of the developed coating strategy.
Article
Biotechnology & Applied Microbiology
Aslihan Kazan, Xihua Hu, Alina Stahl, Heike Frerichs, Irina Smirnova, Ozlem Yesil-Celiktas
Summary: The study developed an alginate-based immobilized enzyme microreactor for the catalytic conversion of ginsenoside Rb1 to more bioactive metabolites, showing enhanced activity compared to conventional approaches. There is potential for the biocatalysis of multiple ginsenosides and pharmaceutically active compounds using this newly developed enzymatic microreactor.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Aslihan Kazan
Summary: This review discusses the recent progress in supercritical microfluidics with a focus on supercritical CO2, presenting its applications in microscale such as extraction of valuable compounds, determination of dissolution characteristic in various solvents, performing chemical reactions, and particle synthesis.
MICROFLUIDICS AND NANOFLUIDICS
(2022)
Correction
Materials Science, Multidisciplinary
Patrina Paraskevopoulou, Irina Smirnova, Tamara Athamneh, Maria Papastergiou, Despoina Chriti, Gregor Mali, Tomaz Cendak, Margarita Chatzichristidi, Grigorios Raptopoulos, Pavel Gurikov
ACS APPLIED POLYMER MATERIALS
(2021)
Article
Materials Science, Paper & Wood
Baldur Schroeter, Velislava P. Yonkova, Noreen A. M. Niemeyer, Isabella Jung, Imke Preibisch, Pavel Gurikov, Irina Smirnova
Summary: The reported approach focuses on synthesizing spherical cellulose beads with high production rates using the jet cutting method. By varying cellulose content and jet cutting process parameters, control over particle size and shape was achieved, resulting in cellulose aerogel particles with high specific surface areas and low bulk densities. Highly spherical particles with high sphericity were obtained over a broad range of achievable sizes, with an optimum observed at 6 wt% cellulose content.
Article
Materials Science, Multidisciplinary
Patrina Paraskevopoulou, Irina Smirnova, Tamara Athamneh, Maria Papastergiou, Despoina Chriti, Gregor Mali, Tomaz Cendak, Margarita Chatzichristidi, Grigorios Raptopoulos, Pavel Gurikov
ACS APPLIED POLYMER MATERIALS
(2020)
Article
Engineering, Biomedical
Fatemeh Sharifi, Ozlem Yesil-Celiktas, Aslihan Kazan, Sushila Maharjan, Saghi Saghazadeh, Keikhosrow Firoozbakhsh, Bahar Firoozabadi, Yu Shrike Zhang
BIO-DESIGN AND MANUFACTURING
(2020)
