Article
Multidisciplinary Sciences
Riku Sakurai, Shuhei Takizawa, Yasuhiro Fukuda, Chika Tada
Summary: This study investigated the cardinal microorganisms in anaerobic scum digestion by enriching scum-degrading microbes through pre-incubation. The results showed that a variety of microorganisms capable of degrading LCFAs, proteins, and sugars are involved in effective scum degradation, with certain species becoming more dominant as the scum-loading rate increased.
Review
Medicine, General & Internal
Sara Deleu, Kathleen Machiels, Jeroen Raes, Kristin Verbeke, Severine Vermeire
Summary: Short-chain fatty acids play important roles in the gut microbiome and intestinal immune system, exerting various effects on host metabolism and immune system. Further research is needed on the cross-feeding mechanisms in the gut microbiome and the therapeutic potential of SCFAs in different disease models.
Article
Biochemistry & Molecular Biology
Zhengqi Zha, Xiaoning Wang, Guoqing Wang, Hongping Yin, Hang Wang
Summary: A synthesized xylan acetate ester, XylA, overcomes the limitations of acetate in the colon and shows potential in alleviating symptoms of chronic kidney disease (CKD) by upregulating short-chain fatty acids (SCFAs), G-protein-coupled receptor 41 (GPR41) expression, inhibiting glomerular cell apoptosis, and promoting proliferation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Multidisciplinary Sciences
Tadashi Takeuchi, Eiji Miyauchi, Takashi Kanaya, Tamotsu Kato, Yumiko Nakanishi, Takashi Watanabe, Toshimori Kitami, Takashi Taida, Takaharu Sasaki, Hiroki Negishi, Shu Shimamoto, Akinobu Matsuyama, Ikuo Kimura, Ifor R. Williams, Osamu Ohara, Hiroshi Ohno
Summary: This study found that the gut microbial metabolite acetate not only increases IgA production in the colon, but also alters the ability of IgA to bind to specific microorganisms. Acetate orchestrates interactions between epithelial and immune cells, induces microbially stimulated CD4 T cells to support T-cell-dependent IgA production, and changes the localization of these bacteria within the colon.
Article
Food Science & Technology
Paul A. Gill, Alexander Bogatyrev, Menno C. van Zelm, Peter R. Gibson, Jane G. Muir
Summary: This study aimed to quantify SCFA in commonly consumed foods and beverages, and explore the pharmacokinetics of consuming oral SCFA from dietary sources. Acetate was found to be the most abundant SCFA detected, with kombucha and vinegar providing high levels of acetate. An acute pharmacokinetic study showed that consuming a vinegar drink significantly increased plasma acetate concentration, indicating the potential for systemic delivery of SCFA through fermented foods and beverages over time.
MOLECULAR NUTRITION & FOOD RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Fang Li, Zhuoran Kang, Xiaojuan Wu, Wei Wu
Summary: Dietary protein plays a crucial role in body growth and shaping the gut microbiota. This study found that oxidation of rice bran protein (RBP) altered the composition of the gut microbiota, improved intestinal barrier function, but had limited effects on short-chain fatty acid production.
Article
Endocrinology & Metabolism
Juulia H. Lautaoja, Thomas M. O'Connell, Sakari Mantyselka, Juuli Perakyla, Heikki Kainulainen, Satu Pekkala, Perttu Permi, Juha J. Hulmi
Summary: The metabolic effects of EL-EPS under high- and low-glucose conditions were evaluated in this study. Results showed that myotubes grown under high-glucose conditions had greater responsiveness to EL-EPS and acetate and branched-chain fatty acids were identified as putative exerkines warranting further research.
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2021)
Article
Biochemistry & Molecular Biology
Ying He, Xie Peng, Yang Liu, Qing Wu, Qiang Zhou, Yingyan Huang, Shiya Liu, Liang Hu, Zhengfeng Fang, Yan Lin, Shengyu Xu, Bin Feng, Jian Li, Xuemei Jiang, Yong Zhuo, De Wu, Lianqiang Che
Summary: This study investigated the effects of long-term maternal intake of inulin on intestinal morphology, permeability, inflammation, and microbiota in offspring rats treated with DSS. The results showed that maternal intake of inulin exacerbated intestinal damage and inflammation, associated with decreased acetate levels and altered intestinal microbiota. These findings contribute to a better understanding of the impact of inulin on intestinal health.
Article
Multidisciplinary Sciences
Kristin J. Skaarud, Johannes R. Hov, Simen H. Hansen, Martin Kummen, Jorgen Valeur, Ingebjorg Seljeflot, Asta Bye, Vemund Paulsen, Knut E. A. Lundin, Marius Troseid, Geir E. Tjonnfjord, Per Ole Iversen
Summary: After allo-HSCT, gut microbiota and short-chain fatty acids were significantly reduced, with no significant differences between the intervention group and controls in investigated parameters. Patients with low microbial diversity at 3 weeks post-HSCT had significantly higher mortality rates, while those with higher abundance of Blautia genus were more likely to survive.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Kuei-Yi Hung, Shu-Yu Wu, Hsin-Ping Pao, Wen- Liao, Shi-Jye Chu
Summary: Recent data suggests that acetate, a type of short-chain fatty acid, shows protective effects against acute lung injury induced by ischemia-reperfusion. Acetate works by inhibiting inflammation and reducing cellular damage.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Food Science & Technology
Lisa F. M. Lee Nen That, Baojun Xu, Jessica Pandohee
Summary: The maturation of omics technologies and bioinformatics tools has greatly advanced prebiotics research. Prebiotics are no longer limited to oligosaccharides, but include various compounds that have been proven to have prebiotic characteristics. The discovery of novel prebiotics requires further understanding of their roles in the microbiota and the host. The relationship between the gut microbiota and the host is crucial for health, and dysbiosis can result in severe illnesses.
CURRENT OPINION IN FOOD SCIENCE
(2022)
Article
Microbiology
Ryo Aoki, Masayoshi Onuki, Koya Hattori, Masato Ito, Takahiro Yamada, Kohei Kamikado, Yun-Gi Kim, Nobuhiro Nakamoto, Ikuo Kimura, Julie M. Clarke, Takanori Kanai, Koji Hase
Summary: The study demonstrates that the commensal microbiome-acetate-FFAR2 molecular circuit improves insulin sensitivity in the liver and prevents the development of NAFLD/NASH. Consumption of prebiotic inulin leads to significant improvements in NAFLD/NASH phenotype, with changes in gut microbiome and increased concentrations of short-chain fatty acids like acetate.
Article
Chemistry, Applied
Azemina Bajramova, Peter Spegel
Summary: This study compares the nutritional value of the fatty acid profiles of superfruits (goji berry, white mulberry, and cranberry) and common fruits (banana, apple, and strawberry). The findings suggest that cranberries and apples have the most beneficial lipid profiles, while superfruits do not show a common and beneficial lipid profile.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Chemistry, Multidisciplinary
Zhixuan Fan, Yunchao Zhao, Xuying Liu, Yu Shi, Dahua Jiang
Summary: In this study, a myristic acid-paraffin wax binary eutectic phase change material was prepared and its phase transition properties, thermal stability, and chemical structure were investigated. The results showed that the binary eutectic PCM exhibited good thermal properties and chemical stability.
Article
Biotechnology & Applied Microbiology
Karolina Szacherska, Krzysztof Moraczewski, Sylwester Czaplicki, Piotr Oleskowicz-Popiel, Justyna Mozejko-Ciesielska
Summary: This study evaluated the use of short- and medium-chain fatty acids (SMCFAs) derived from acid whey fermentation for polyhydroxyalkanoate (PHA) production. Pseudomonas sp. GL06 showed the highest tolerance to SMCFAs and produced PHAs with the highest productivity. Further research revealed that PHA monomeric composition varied with bacterial strain and growth medium. The copolymer made of 3-hydroxybutyric, -hexanoic, -octanoic, -decanoic, and -dodecanoic exhibited promising properties.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Engineering, Environmental
B. Conall Holohan, M. Salome Duarte, M. Alejandra Szabo-Corbacho, Ana J. CavaleiroAna, Andreia F. Salvador, M. Alcina Pereira, Ryan M. ZielsRyan, Carla T. M. J. Frijters, Santiago Pacheco-Ruiz, Marta Carballa, Diana Z. Sousa, Alfons J. M. Stams, Vincent O'Flaherty, Jules B. van Lier, M. Madalena Alves
Summary: Lipids in wastewater treatment plants pose challenges, but can be effectively treated using appropriate strategies and technologies. Understanding microbial communities and interactions enhances understanding of lipid degradation in anaerobic reactors.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Anna Doloman, Sjef Boeren, Charles D. Miller, Diana Z. Sousa
Summary: Results from this study shed light on the importance of complex microbial cultures in anaerobic environments, which can help in developing reliable metabolic models. Further, the interactions between syntrophic bacteria need to be further explored for a better understanding of their relationships.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Sara Benito-Vaquerizo, Ivette Parera Olm, Thijs de Vroet, Peter J. Schaap, Diana Z. Sousa, Vitor A. P. Martins dos Santos, Maria Suarez-Diez
Summary: This study used computational and experimental methods to study the metabolism of Anaerotignum neopropionicum, a propionate-producing bacterium. The construction of the genome-scale metabolic model iANEO_SB607 provided insight into the acrylate pathway for fermentation of ethanol into propionate, shedding light on the energetic aspects of the cell.
MICROBIAL CELL FACTORIES
(2022)
Article
Chemistry, Multidisciplinary
Gilberto Martins, Sara Campos, Ana Ferreira, Rita Castro, Maria Salome Duarte, Ana J. Cavaleiro
Summary: The developed mathematical model serves as a decision-supporting tool for defining the best strategies for in situ bioremediation of contaminated sites. By integrating the contributions of various physicochemical and biological processes, it facilitates the simulation and comparison of different bioremediation scenarios, providing useful support for the management of contaminated sites.
APPLIED SCIENCES-BASEL
(2022)
Review
Microbiology
Ana R. Castro, Gilberto Martins, Andreia F. Salvador, Ana J. Cavaleiro
Summary: Waste and wastewater containing hydrocarbons are produced by oil-based industries worldwide, leading to severe environmental contamination. Anaerobic microorganisms have the ability to biodegrade petroleum hydrocarbons, and iron compounds play multiple roles in this process, promoting hydrocarbon degradation.
Article
Biotechnology & Applied Microbiology
Maximilienne T. Allaart, Martijn Diender, Diana Z. Sousa, Robbert Kleerebezem
Summary: Carboxydotrophic metabolism is gaining interest for its applications in gas fermentation technology, where carbon monoxide is converted into fuels and commodities. Acetogenic carboxydotrophs, such as C. autoethanogenum, play a crucial role in this process. To cope with the toxicity of CO, these organisms employ a form of overflow metabolism that allows them to efficiently use CO at varying substrate influxes. The study focuses on how C. autoethanogenum utilizes acetate reduction to ethanol as a form of overflow metabolism when grown solely on CO and syngas mixtures.
MICROBIAL BIOTECHNOLOGY
(2023)
Article
Agricultural Engineering
Sara Cantera, Francesca Di Benedetto, Ben F. Tumulero, Diana Z. Sousa
Summary: This study explores a novel conversion of CO2 into high-value chemicals hydroxyectoine and ectoine in the pharmaceutical industry. Through literature search and genomic mining, 11 species of microbes capable of using CO2 and H-2 with the genes for ectoine synthesis were identified. Laboratory tests confirmed that Hydrogenovibrio marinus, Rhodococcus opacus, and Hydrogenibacillus schlegelii are the most promising bacteria for CO2 to ectoines bioconversion. These results provide the first proof of a novel valorization platform for CO2 and lay the foundation for a new economic niche in CO2 recircularization into pharmaceuticals.
BIORESOURCE TECHNOLOGY
(2023)
Article
Microbiology
Nicolas Vecchini Santaella, Diana Z. Sousa, Alfons J. M. Stams
Summary: Based on genomic analysis and phenotypic results, strain AMPT (DSM 21394T=JCM 35360T) should be classified as a new bacterial species in the genus Moorella, named Moorella caeni sp. nov.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2023)
Article
Engineering, Environmental
Le Min Chen, Stefan de Bruin, Mario Pronk, Diana Z. Sousa, Mark C. M. van Loosdrecht, Yuemei Lin
Summary: This study investigates the sialylation and sulfation of anionic glycoconjugates in anaerobic and aerobic granular sludge. The results showed that these two glycoconjugate modifications commonly occur in the extracellular polymericsubstances (EPS) of both types of sludge. Mass spectrometry analysis and gene identification provided insights into the distribution and synthesis of these modifications in the sludge communities.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Review
Environmental Sciences
M. Salome Duarte, Gilberto Martins, Pedro Oliveira, Bruno Fernandes, Eugenio C. Ferreira, M. Madalena Alves, Frederico Lopes, M. Alcina Pereira, Paulo Novais
Summary: Wastewater treatment companies are facing challenges in energy efficiency optimization, meeting stricter water quality standards, and resource recovery potential. Computational models have been recognized as effective tools for addressing these challenges and improving economic and operational efficiencies of wastewater treatment plants. Deterministic, stochastic, and time series-based models have been developed to predict the performance of wastewater treatment plants. Mechanistic models, although dominant, may introduce model structure uncertainty and require frequent calibration. Data-driven models, which search for relationships in available data, are becoming more attractive with the increasing amount of data. This review discusses the implementation of machine learning models for predicting WWTP effluent characteristics, wastewater inflows, anomaly detection, and energy consumption optimization. Hybrid models, merging mechanistic and machine learning models, are presented as a promising approach. The review also assesses gaps and future directions in the implementation of mathematical modeling in wastewater treatment processes, focusing on explainability of data-driven models and the use of Transfer Learning processes.
Article
Biotechnology & Applied Microbiology
Karel Olavarria, Marco V. Becker, Diana Z. Sousa, Mark C. M. van Loosdrecht, S. Aljoscha Wahl
Summary: This study proposes a novel metabolic engineering strategy to achieve efficient anaerobic production of poly-(R)-3-hydroxybutyrate (PHB) in Escherichia coli. The strategy involves rerouting the glycolytic flux through non-oxidative glycolysis and coupling PHB synthesis with NADH re-oxidation. Stoichiometric analysis and various thermodynamic approaches were used to assess the feasibility and bottlenecks of the engineered pathway.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2023)
Article
Energy & Fuels
Joao Oliveira, Jose C. Costa, Ana J. Cavaleiro, Maria Alcina Pereira, Maria Madalena Alves
Summary: This study evaluates the impact of inoculum type, pre-consumption of residual substrate, and the ratio of headspace volume to working volume on Biochemical Methane Potential (BMP) measurements. The findings suggest that degassing and discounting methane produced in blanks are essential for accurate results. The residual substrate concentration of different inocula also plays a role in the determination.
Article
Biochemistry & Molecular Biology
Joao C. Sequeira, Miguel Rocha, M. Madalena Alves, Andreia F. Salvador
Summary: Omics and meta-omics technologies provide powerful approaches to explore microbial functions. However, the analysis of large and complex datasets is often challenging. In this study, three novel bioinformatics tools were developed to facilitate functional annotation and metabolic pathway analysis. These tools are user-friendly and valuable resources for interpreting microbial activities in nature and biotechnological processes.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
