Article
Cell Biology
Constantin Blochl, Di Wang, Katarina Madunic, Guinevere S. M. Lageveen-Kammeijer, Christian G. Huber, Manfred Wuhrer, Tao Zhang
Summary: AML presents challenges in treatment due to high rates of relapse or refractory disease. Recent studies have highlighted the importance of protein glycosylation in AML pathobiology, with specific glycan epitopes linked to disease outcome. This exploratory glycomics study identified distinct glycan structures in AML cell lines, providing insights into potential drivers of glycan phenotype.
Article
Chemistry, Analytical
Amanda Helms, Edwin E. E. Escobar, Saulius Vainauskas, Christopher H. H. Taron, Jennifer S. S. Brodbelt
Summary: The complete characterization of O-glycopeptides, including identification of peptides, localization of glycosites, and mapping of glycans, has been a challenging task in O-glycoproteomics. This study successfully applied a strategy combining the use of O-glycoprotease IMPa and HCD-triggered UVPD to achieve the complete characterization of O-glycopeptides. This approach allowed the localization of multiple adjacent or proximal O-glycosites and the identification of a previously unknown glycosite on etanercept.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Miyang Li, Ting-jia Gu, Xiaorong Lin, Lingjun Li
Summary: Glycosylation plays a key role in physiological functions, but high-throughput quantitative analysis of O-glycans has been overlooked. A multiplexed isobaric labeling method has been introduced to release and label O-glycans in a single reaction, enabling the processing of four samples simultaneously for accurate relative quantification.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Zahra Rashidijahanabad, Sherif Ramadan, Nicholas A. O'Brien, Athar Nakisa, Shuyao Lang, Howard Crawford, Jeffrey C. Gildersleeve, Xuefei Huang
Summary: Researchers have successfully developed a sLe(a)-based anticancer vaccine, which generated strong and long-lasting antibody responses in mice, selectively killed cancer cells, and significantly reduced tumor development.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Oncology
Fabio Dall'Olio, Michela Pucci, Nadia Malagolini
Summary: B4GALNT2 is an enzyme that is associated with better prognosis and cancer-restraining activity in colorectal cancer, with its expression influencing malignancy and gene expression profile. The Sd(a) antigen and sialyl Lewis(x/a) antigens play crucial roles in cancer, with their biosynthesis being mutually exclusive.
Article
Chemistry, Physical
David Teze, Joan Coines, Folmer Fredslund, Kshatresh D. Dubey, Gonzalo N. Bidart, Paul D. Adams, John E. Dueber, Birte Svensson, Carme Rovira, Ditte H. Welner
Summary: The study found that UGT1 from Polygonum tinctorium can catalyze N-, O-, or S-glycosylation with similar rates, and elucidated the molecular basis of N-, O-, and S-specificities using experimental mutagenesis and simulations. It also showed that the active site can be engineered to increase or favor one of the three glycosylation activities over another, leading to the design of more active and specific enzyme variants for glycoside production.
Article
Cell Biology
Dan Zhang, Yihong Zhang, Xiuqun Zou, Mengying Li, Hui Zhang, Yaning Du, Jiamin Wang, Chicheng Peng, Chunyan Dong, Zhaoyuan Hou
Summary: The gene encoding the key GlcNAc sulfation enzyme CHST2 is induced by Snail in multiple breast cancer cells. Depletion of CHST2 inhibits breast cancer cell migration and metastasis, while overexpression of CHST2 promotes cell migration and lung metastasis in nude mice. Blocking the cell surface MECA79 antigen and sulfation inhibitor sodium chlorate effectively inhibit the cell migration induced by CHST2. These findings provide new insights into the biology of Snail/CHST2/MECA79 axis in breast cancer progression and metastasis, and potential therapeutic strategy for breast cancer metastasis.
CELL DEATH & DISEASE
(2023)
Article
Chemistry, Multidisciplinary
Jialin Liu, Bo Cheng, Xinqi Fan, Xinyue Zhou, Jiankun Wang, Wen Zhou, Hengyu Li, Wenfeng Zeng, Pengyuan Yang, Xing Chen
Summary: Proteins are extensively modified with glycans of different structures, making it challenging to map the protein glycosylation landscape. This study presents Click-iG, a method that enables the simultaneous enrichment and profiling of three types of intact glycopeptides using metabolic labeling of glycans, optimized mass spectrometry, and tailored software analysis. Click-iG was able to identify thousands of intact glycosites in cell lines and living mice, providing comprehensive coverage of the protein glycosylation landscape and laying the foundation for investigating crosstalk between different glycosylation pathways.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Immunology
Gurdeep Chahal, Macarena P. Quintana-Hayashi, Meztlli O. Gaytan, John Benktander, Medea Padra, Samantha J. King, Sara K. Linden
Summary: The research found that Streptococcus oralis binding to oral mucins requires specific carbohydrate structures, with three distinct carbohydrate binding specificities involved in the binding. Additionally, the mechanisms of binding MUC5B and MUC7 differ, and efficient binding requires a gene encoding AsaA.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Ignacio Sanz-Martinez, Ana Garcia-Garcia, Tomas Tejero, Ramon Hurtado-Guerrero, Pedro Merino
Summary: Protein O-fucosyltransferase 2 (PoFUT2) is an important glycosyltransferase that recognizes a diverse range of TSRs through water-mediated interactions. Water molecules not only play a role in ligand recognition but also in the catalytic mechanism of PoFUT2.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Review
Microbiology
Daniela Rothschild-Rodriguez, Morgen Hedges, Merve Kaplan, Sercan Karav, Franklin L. Nobrega
Summary: The interaction between the gut mucosa and the bacterial component of the microbiota in the human gastrointestinal tract plays a crucial role in digestion, immune regulation, and overall health. Gut bacteria residing in the mucus layer can influence the integrity of the mucosal barrier, affecting health or disease progression. The viral members, particularly bacteriophages, also have the potential to interact with gut mucus, but their effects remain largely unexplored. This review discusses the role of bacteriophages in influencing mucosal integrity and their potential impact on health and disease states.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Ana Magalhaes, Henrique O. Duarte, Celso A. Reis
Summary: O-glycosylation is a frequent post-translational modification of proteins with functional implications in both physiological and disease contexts. The biosynthesis depends on regulation of cellular glycosylation machinery, affecting organ-, tissue-, and cell-specific functions. Diseases triggered or modulated by aberrant O-glycosylation include genetic disorders, infectious diseases, and cancer, with potential therapeutic strategies focusing on O-glycans and their pathways.
MOLECULAR ASPECTS OF MEDICINE
(2021)
Article
Chemistry, Physical
Xiaoju Zheng, Lin Zhu, Tianlu Li, Wenjia Xu, Dongke Liu, Juzheng Sheng, Hongzhi Cao, Yikang Shi, Fengshan Wang
Summary: Glycosylation provides a solution for modifying peptide drugs, improving stability and receptor selectivity. The study introduces a enzymatic modular assembly strategy for the synthesis of active glycopeptides, which significantly increases the half-lives of the peptides in human serum.
Article
Chemistry, Analytical
Juhi Samal, Tana V. Palomino, Judy Chen, David C. Muddiman, Tatiana Segura
Summary: N-linked glycosylation is a complex protein modification that plays a role in cellular signaling and metabolism. Aberrant protein glycosylation is a characteristic feature of many diseases. Spatial profiling of N-glycans through imaging on tissue sections can be used for disease diagnosis. Infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) is a sensitive technique that can be used for mass spectrometry imaging.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Siyuan Kong, Quanqing Zhang, Lujie Yang, Yuanyu Huang, Mingqi Liu, Guoquan Yan, Huanhuan Zhao, Mengxi Wu, Xiangmin Zhang, Pengyuan Yang, Weiqian Cao
Summary: A new enrichment strategy using a boronic acid-functionalized mesoporous graphene-silica composite is proposed for isolating intact glycopeptides, showing effectiveness and specificity in glycosylation analysis.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Florent Colomb, Marie-Ange Krzewinski-Recchi, Agata Steenackers, Audrey Vincent, Anne Harduin-Lepers, Philippe Delannoy, Sophie Groux-Degroote
BIOCHEMICAL JOURNAL
(2017)
Article
Multidisciplinary Sciences
Justine H. Dewald, Sumeyye Cavdarli, Agata Steenackers, Clement P. Delannoy, Marlene Mortuaire, Corentin Spriet, Maxence Noel, Sophie Groux-Degroote, Philippe Delannoy
Article
Biochemistry & Molecular Biology
Sophie Groux-Degroote, Celine Schulz, Virginie Cogez, Maxence Noel, Lucie Portier, Dorothee Vicogne, Carlos Solorzano, Fabio Dall'Olio, Agata Steenackers, Marlene Mortuaire, Mariano Gonzalez-Pisfil, Melanie Henry, Francois Foulquier, Laurent Heliot, Anne Harduin-Lepers
Article
Cell Biology
Marie Bobowski-Gerard, Francesco Paolo Zummo, Bart Staels, Philippe Lefebvre, Jerome Eeckhoute
Article
Biochemistry & Molecular Biology
Sumeyye Cavdarli, Justine H. Dewald, Nao Yamakawa, Yann Guerardel, Mickael Terme, Jean-Marc Le Doussal, Philippe Delannoy, Sophie Groux-Degroote
GLYCOCONJUGATE JOURNAL
(2019)
Review
Biochemistry & Molecular Biology
Sumeyye Cavdarli, Sophie Groux-Degroote, Philippe Delannoy
Article
Biochemistry & Molecular Biology
Sumeyye Cavdarli, Nao Yamakawa, Charlotte Clarisse, Kazuhiro Aoki, Guillaume Brysbaert, Jean-Marc Le Doussal, Philippe Delannoy, Yann Guerardel, Sophie Groux-Degroote
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Vanessa Dubois, Celine Gheeraert, Wouter Vankrunkelsven, Julie Dubois-Chevalier, Helene Dehondt, Marie Bobowski-Gerard, Manjula Vinod, Francesco Paolo Zummo, Fabian Guiza, Maheul Ploton, Emilie Dorchies, Laurent Pineau, Alexis Boulinguiez, Emmanuelle Vallez, Eloise Woitrain, Eric Bauge, Fanny Lalloyer, Christian Duhem, Nabil Rabhi, Ronald E. van Kesteren, Cheng-Ming Chiang, Steve Lancel, Helene Duez, Jean-Sebastien Annicotte, Rejane Paumelle, Ilse Vanhorebeek, Greet Van den Berghe, Bart Staels, Philippe Lefebvre, Jerome Eeckhoute
MOLECULAR SYSTEMS BIOLOGY
(2020)
Review
Biochemistry & Molecular Biology
Sophie Groux-Degroote, Dorothee Vicogne, Virginie Cogez, Celine Schulz, Anne Harduin-Lepers
Summary: The study focused on the expression of blood group carbohydrate antigens Sd(a) and SLe(x) in normal and cancerous colonic tissues, as well as the potential prognostic value of high levels of B4GALNT2 gene expression in colon cancer. Despite the association between elevated B4GALNT2 expression and better prognosis, the molecular mechanisms regulating the expression of these carbohydrate antigens remain poorly understood.
Review
Biochemistry & Molecular Biology
Sophie Groux-Degroote, Philippe Delannoy
Summary: Aberrant expression of glycosphingolipids is closely linked with malignant properties of cancer cells, making them targets for immunotherapy. Various strategies, including monoclonal antibodies, vaccines, and immune effector-cell therapy, have shown promising results in targeting these tumor-associated gangliosides. Adoptive cellular therapy with engineered T cells expressing chimeric antigen receptors is a particularly focused approach in this field.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Medicine, Research & Experimental
Sophie Groux-Degroote, Francois Foulquier, Sumeyye Cavdarli, Philippe Delannoy
Summary: Glycosylation is a crucial modification of proteins and lipids, primarily occurring in the endoplasmic reticulum and Golgi apparatus. Dysfunctions in glycosylation have been linked to disruptions in cellular metabolism, thereby contributing to the development of cancers.
M S-MEDECINE SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Cindy Wavelet-Vermuse, Sophie Groux-Degroote, Dorothee Vicogne, Virginie Cogez, Giulia Venturi, Marco Trinchera, Guillaume Brysbaert, Marie-Ange Krzewinski-Recchi, Elsa Hadj Bachir, Celine Schulz, Audrey Vincent, Isabelle Van Seuningen, Anne Harduin-Lepers
Summary: The research identified the transcriptional regulation of the human B4GALNT2 gene, showing that ETS1 and SP1 transcription factors play important roles and the essential promoter activity is located in the region from -72 to +12. These findings provide insights into the regulatory mechanisms of B4GALNT2.
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2021)
Article
Oncology
Angelina Kasprowicz, Groux-Degroote Sophie, Chann Lagadec, Philippe Delannoy
Summary: GD3 synthase is crucial for the biosynthesis of gangliosides, and targeting it can reduce malignant properties of tumors associated with gangliosides, such as migration, invasion, sternness, and epithelial-mesenchymal transition.
Article
Biochemistry & Molecular Biology
Clement Delannoy, Chin Huang, Bernadette Coddeville, Jian-You Chen, Dounia Mouajjah, Sophie Groux-Degroote, Anne Harduin-Lepers, Kay-Hooi Khoo, Yann Guerardel, Elisabeth Elass-Rochard