Article
Physics, Multidisciplinary
Viviana Claveria, Philippe Connes, Luca Lanotte, Celine Renoux, Philippe Joly, Romain Fort, Alexandra Gauthier, Christian Wagner, Manouk Abkarian
Summary: Red blood cells in sickle cell anemia exhibit more heterogeneous physical properties compared to healthy red blood cells, with differences in adhesiveness, rigidity, density, size, and shape. The segregation of sickle red blood cells in micron-sized channels was investigated, with findings showing that low-density cells tended to remain in the center of the channel while the densest cells segregated towards the walls. Aggregation inhibited segregation and increased the cell depleted layer thickness, potentially playing a protective role against vaso-occlusion in sickle cell anemia patients.
FRONTIERS IN PHYSICS
(2021)
Article
Chemistry, Multidisciplinary
Tamir Tsohar, Shaul Beyth, Alexander Gural, Dan Arbell, Saul Yedgar, Gregory Barshtein
Summary: This study investigated the impact of non-physiological mechanical stress during surgery on red blood cell deformability. The findings showed that mechanical stress reduces cell deformability, increases the percentage of undeformable cells, and leads to hemolysis of rigid cells. The final outcome depends on the initial concentration of undeformable cells in the sample.
APPLIED SCIENCES-BASEL
(2022)
Article
Multidisciplinary Sciences
Mario Gutierrez, Mark Shamoun, Katie Giger Seu, Tyler Tanski, Theodosia A. Kalfa, Omolola Eniola-Adefeso
Summary: In this study, a parameterization model of ektacytometry was used to quantify the bulk rigidity of rigid red blood cell populations in sickle cell disease patients. By coupling this model with an artificially rigid RBC model, an innovative system was formulated to estimate the average rigidity of rigid RBC populations in SCD blood, potentially offering valuable insights into disease severity assessment and treatment.
SCIENTIFIC REPORTS
(2021)
Article
Physiology
Evrim Goksel, Elif Ugurel, Elie Nader, Camille Boisson, Ingrid Muniansi, Philippe Joly, Celine Renoux, Alexandra Gauthier, Philippe Connes, Ozlem Yalcin
Summary: In this study, the effects of AC signaling pathway modulation and different PDE modulatory molecules on RBC deformability in SCD patients were investigated. The results showed that different drugs had distinct effects on RBC deformability, suggesting the potential of developing AC signaling pathway-targeting drugs for SCD treatment. However, more research is needed to identify the differences in the responses of sickle RBCs between patients.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Silvie Suriany, Iris Xu, Honglei Liu, Pinar Ulker, G. Esteban Fernandez, Richard Sposto, Matthew Borzage, Rosalinda Wenby, Herbert J. Meiselman, Henry Jay Forman, Thomas D. Coates, Jon A. Detterich
Summary: Sickle cell anemia patients exhibit increased basal nitric oxide (NO·) production in RBCs, with morphological changes during shear stress. NO· production is influenced by NOS, and sickle-shaped RBCs with inclusions show higher NO· production during shear.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Cell Biology
Elif Ugurel, Evrim Goksel, Neslihan Cilek, Elif Kaga, Ozlem Yalcin
Summary: This study investigates the role of the cAMP signaling mechanism in modulating shear-induced red blood cell (RBC) deformability and examines changes in the phosphorylation of the RBC proteome. The inhibition of signaling molecules significantly deteriorates shear-induced RBC deformability. The cAMP/PKA pathway regulates RBC deformability by triggering significant alterations in the phosphorylation state of RBCs.
Article
Biochemical Research Methods
Xingjian Zhang, Trevor Chan, Judith Carbonella, Xiangyu Gong, Noureen Ahmed, Chang Liu, Israel Demandel, Junqi Zhang, Farzana Pashankar, Michael Mak
Summary: Sickle cell disease is a genetic condition that can cause life-threatening symptoms due to vaso-occlusive crisis. We designed a microfluidic-informatics analytical system to quantify the occlusion of sickle cell disease, and found an increase in physical occlusion events in the most severe patients. By using bioinformatics and modeling, we were able to assess the severity of the disease in vitro for individual patients. We also demonstrated the potential impact of hydration on reducing disease severity in high-risk patients. Overall, our device provides an easy-to-use assay for quick occlusion information extraction and shows promise as a platform for testing therapeutic strategies.
Review
Cell Biology
Neslihan Cilek, Elif Ugurel, Evrim Goksel, Ozlem Yalcin
Summary: Intracellular signaling mechanisms in red blood cells (RBCs) involve protein kinases and phosphatases, which regulate the physiological properties of the cell in response to various stimuli. Protein phosphorylation plays a crucial role in intracellular signal transduction, volume regulation, and cytoskeletal organization in RBCs. The structural organization of the membrane and cytoskeleton determines RBC deformability and allows them to pass through narrow capillaries under shear stress.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Yifan Liu, Fengxian Xin
Summary: A cyclic coupling computational model is developed to investigate the large deformation of swollen red blood cells induced by the acoustic radiation force. The model accurately predicts the acoustic deformability of cells by calculating the acoustic radiation force and mechanical response of the cell membrane. The results also demonstrate that the deformation of multiple cells is influenced by cell-cell interaction.
MICROFLUIDICS AND NANOFLUIDICS
(2022)
Article
Physiology
Emeric Stauffer, Solene Poutrel, Giovanna Cannas, Alexandra Gauthier, Romain Fort, Yves Bertrand, Celine Renoux, Philippe Joly, Camille Boisson, Arnaud Hot, Laure Peter-Derex, Vincent Pialoux, Thierry PetitJean, Philippe Connes
Summary: This study found that nocturnal hypoxemia in SCD patients may be related to hemolytic complications, while there was no association found between OSA and clinical severity.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Dina Baier, Torsten Mueller, Thomas Mohr, Ursula Windberger
Summary: The study investigated the apparent Young's modulus and adhesion properties of different animal red blood cells, finding that the Young's modulus decreased with increasing temperature in all cell types, while adhesion increased with temperature in mammalian cells and correlated with reported membrane sialic acid concentrations.
Article
Physiology
Madeleine Lu, Celeste K. Kanne, Riley C. Reddington, Dalia L. Lezzar, Vivien A. Sheehan, Sergey S. Shevkoplyas
Summary: The study introduces a microfluidic device capable of evaluating red blood cell deformability and adhesiveness, demonstrating slower perfusion rates for sickle red blood cells compared to normal red blood cells in LN-coated networks. This indicates the potential utility of the device for assessing novel therapies in SCD patients.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Travis Nemkov, Sarah C. Skinner, Elie Nader, Davide Stefanoni, Melanie Robert, Francesca Cendali, Emeric Stauffer, Agnes Cibiel, Camille Boisson, Philippe Connes, Angelo D'Alessandro
Summary: A controlled, high-intensity cycling test resulted in decreased RBC deformability, increased microparticle generation, oxidative stress, and activation of membrane lipid remodeling mechanisms. Metabolites involved in CoA synthesis and carnitine conversion were significantly correlated with RBC properties during exercise.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Brenda J. Green, Margherita Marazzini, Ben Hershey, Amir Fardin, Qingsen Li, Zongjie Wang, Giovanni Giangreco, Federica Pisati, Stefano Marchesi, Andrea Disanza, Emanuela Frittoli, Emanuele Martini, Serena Magni, Galina Beznoussenko, Claudio Vernieri, Riccardo Lobefaro, Dario Parazzoli, Paolo Maiuri, Kristina Havas, Mahmoud Labib, Sara Sigismund, Pier Paolo Di Fiore, Rosalind H. Gunby, Shana O. Kelley, Giorgio Scita
Summary: The PillarX device is a bimodular microfluidic device that can analyze circulating tumor cell (CTC) clusters based on their size, deformability, and epithelial marker expression. It captures and separates clusters based on their size and deformability, and can analyze clusters driven by different cell-cell adhesion protein expression. The device has been validated in mouse models and patients of metastatic breast cancer.
Article
Hematology
William J. Wulftange, Erdem Kucukal, Yuncheng Man, Ran An, Karamoja Monchamp, Charlotte D. Sevrain, Himanshu R. Dashora, Amma T. Owusu-Ansah, Allison Bode, Anton Ilich, Jane A. Little, Nigel S. Key, Umut A. Gurkan
Summary: This study utilized a biomimetic microfluidic device to investigate the role of endothelial cell damage and red blood cell adhesion in sickle cell disease, and found that the use of antithrombin-III (ATIII) can alleviate these symptoms.
BRITISH JOURNAL OF HAEMATOLOGY
(2022)
Letter
Hematology
Ann K. Malinowski, Patrina Cheung, Junmin Yang, Rohan D'Souza, Nadine Shehata, Richard Ward, Kevin H. M. Kuo, Kellie Murphy
BRITISH JOURNAL OF HAEMATOLOGY
(2019)
Article
Cardiac & Cardiovascular Systems
Nadine Shehata, Nikhil Mistry, Bruno R. da Costa, Tiago V. Pereira, Richard Whitlock, Gerard F. Curley, David A. Scott, Gregory M. T. Hare, Peter Juni, C. David Mazer
EUROPEAN HEART JOURNAL
(2019)
Article
Engineering, Biomedical
Feng Lin, Yue Shao, Xufeng Xue, Yi Zheng, Zida Li, Chunyang Xiong, Jianping Fu
ACTA BIOMATERIALIA
(2019)
Article
Hematology
Lani Lieberm, Andreas Greinacher, Michael F. Murphy, James Bussel, Tamara Bakchoul, Stacy Corke, Mette Kjae, Jens Kjeldsen-Kragh, Gerald Bertrand, Dick Oepkes, Jillian M. Bake, Heather Hum, Edwin Masse, Cecile Kapla, Donald M. Arnold, Shoma Baidya, Greg Ryan, Helen Savoia, Denise Landry, Nadine Shehata
BRITISH JOURNAL OF HAEMATOLOGY
(2019)
Review
Obstetrics & Gynecology
Ann Kinga Malinowski, Rohan D'Souza, Khalid S. Khan, Nadine Shehata, Marcin Malinowski, Jahnavi Daru
GYNECOLOGIC AND OBSTETRIC INVESTIGATION
(2019)
Article
Instruments & Instrumentation
Abhay Kotnala, Yi Zheng, Jianping Fu, Wei Cheng
REVIEW OF SCIENTIFIC INSTRUMENTS
(2019)
Article
Multidisciplinary Sciences
Yi Zheng, Xufeng Xue, Yue Shao, Sicong Wang, Sajedeh Nasr Esfahani, Zida Li, Jonathon M. Muncie, Johnathon N. Lakins, Valerie M. Weaver, Deborah L. Gumucio, Jianping Fu
Article
Cell Biology
Di Chen, Na Sun, Lei Hou, Rachel Kim, Jared Faith, Marianna Aslanyan, Yu Tao, Yi Zheng, Jianping Fu, Wanlu Liu, Manolis Kellis, Amander Clark
Article
Multidisciplinary Sciences
Y. Zheng, X. Xue, A. M. Resto-Irizarry, Z. Li, Y. Shao, Y. Zheng, G. Zhao, J. Fu
Article
Biochemical Research Methods
Yi Zheng, Yue Shao, Jianping Fu
Summary: This study presents a microfluidic device for modeling human embryo development, derived from human pluripotent stem cells. The resulting structures exhibit molecular and cellular features resembling early post-implantation human embryo development. The microfluidic model is compatible with high-throughput studies and various analytical techniques.
Article
Engineering, Biomedical
Kejie Chen, Yi Zheng, Xufeng Xue, Yue Liu, Agnes M. Resto Irizarry, Huaijing Tang, Jianping Fu
Summary: Human embryonic stem cells have the ability to self-organize and form patterned tissues, and they can be manipulated in vitro to generate embryonic-like structures. A 3D suspension culture system has been developed to efficiently produce postimplantation, pre-gastrulation embryonic-like tissues, with the size of the initial cell seeding density impacting the development trajectory and resulting tissue types.
Editorial Material
Multidisciplinary Sciences
Yi Zheng, Jianping Fu
Summary: Researchers have successfully generated human blastocyst-like structures from cells in a dish, which provides a valuable model for studying human embryology.
Article
Cell & Tissue Engineering
Yi Zheng, Robin Zhexuan Yan, Shiyu Sun, Mutsumi Kobayashi, Lifeng Xiang, Ran Yang, Alexander Goedel, Yu Kang, Xufeng Xue, Sajedeh Nasr Esfahani, Yue Liu, Agnes M. Resto Irizarry, Weisheng Wu, Yunxiu Li, Weizhi Ji, Yuyu Niu, Kenneth R. Chien, Tianqing Li, Toshihiro Shioda, Jianping Fu
Summary: Despite limited understanding of early human development, studying stem cell-derived embryo-like structures can help fill the knowledge gap. This study analyzed the transcriptomes of a human embryoid model at different time points, revealing the critical role of NODAL signaling in mesoderm and primordial germ cell specification. The findings propose stringent criteria for distinguishing between human blastocyst trophectoderm and early amniotic ectoderm cells.
Article
Cell Biology
Agnes M. Resto Irizarry, Sajedeh Nasr Esfahani, Yi Zheng, Robin Zhexuan Yan, Patrick Kinnunen, Jianping Fu
Summary: The human embryo is a complex structure that develops through cell-level decisions guided by genetic programs and interactions. Researchers use human stem cells to generate embryo models for studying embryogenic developmental steps, requiring computational and imaging tools for detailed cell-level dynamics. Video analysis pipelines incorporating machine learning methods can characterize the process of self-organization in stem cell-based embryo models, providing insights into key embryonic events for future mechanistic studies.
INTEGRATIVE BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Ran Yang, Alexander Goedel, Yu Kang, Chenyang Si, Chu Chu, Yi Zheng, Zhenzhen Chen, Peter J. Gruber, Yao Xiao, Chikai Zhou, Nevin Witman, Chuen-Yan Leung, Yongchang Chen, Jianping Fu, Weizhi Ji, Fredrik Lanner, Yuyu Niu, Kenneth Chien
Summary: This study reveals that ISL1 controls a gene regulatory network in the amnion of non-human primate embryos, crucial for mesoderm formation. The research highlights the significance of amnion as a signaling center during primate embryogenesis and the potential of in vitro primate models to dissect the genetics of early human embryonic development.
NATURE COMMUNICATIONS
(2021)
