Optimization of genome editing through CRISPR-Cas9 engineering

Spell Checking Nature: Versatility of CRISPR/Cas9 for Developing Treatments for Inherited Disorders

(2016) Daria Wojtal et al.   AMERICAN JOURNAL OF HUMAN GENETICS

Structure and Engineering of Francisella novicida Cas9

(2016) Hisato Hirano et al.   CELL

Genome engineering through CRISPR/Cas9 technology in the human germline and pluripotent stem cells

(2016) R. Vassena et al.   HUMAN REPRODUCTION UPDATE

Combinatorial optimization of CRISPR/Cas9 expression enables precision genome engineering in the methylotrophic yeast Pichia pastoris

(2016) Astrid Weninger et al.   JOURNAL OF BIOTECHNOLOGY

CRISPR/Cas9 corrects retinal dystrophy in rats

(2016) Dustin M. Graham   LAB ANIMAL

Streptococcus thermophilus CRISPR-Cas9 Systems Enable Specific Editing of the Human Genome

(2016) Maximilian Müller et al.   MOLECULAR THERAPY

The domestication of Cas9

(2016) Fyodor Urnov   NATURE

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

Cas9 loosens its grip on off-target sites

(2016) Christopher E Nelson et al.   NATURE BIOTECHNOLOGY

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

(2016) John G Doench et al.   NATURE BIOTECHNOLOGY

Knock-in of large reporter genes in human cells via CRISPR/Cas9-induced homology-dependent and independent DNA repair

(2016) Xiangjun He et al.   NUCLEIC ACIDS RESEARCH

Long-term dual-color tracking of genomic loci by modified sgRNAs of the CRISPR/Cas9 system

(2016) Shipeng Shao et al.   NUCLEIC ACIDS RESEARCH

Repurposing the CRISPR-Cas9 system for targeted DNA methylation

(2016) Aleksandar Vojta et al.   NUCLEIC ACIDS RESEARCH

The CRISPR-Cas9 technology: Closer to the ultimate toolkit for targeted genome editing

(2016) Francis Quétier   PLANT SCIENCE

Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM

(2016) Alan S. L. Wong et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cas9, poised for DNA cleavage

(2016) H. Chen et al.   SCIENCE

Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage

(2016) F. Jiang et al.   SCIENCE

Advances in therapeutic CRISPR/Cas9 genome editing

(2016) Nataša Savić et al.   Translational Research

ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes

(2016) Kazuto Yoshimi et al.   Nature Communications

Multiplexed pancreatic genome engineering and cancer induction by transfection-based CRISPR/Cas9 delivery in mice

(2016) Roman Maresch et al.   Nature Communications

Improved Genome Editing Efficiency and Flexibility Using Modified Oligonucleotides with TALEN and CRISPR-Cas9 Nucleases

(2016) Jean-Baptiste Renaud et al.   Cell Reports

A Biophysical Model of CRISPR/Cas9 Activity for Rational Design of Genome Editing and Gene Regulation

(2016) Iman Farasat et al.   PLoS Computational Biology

Exploiting the CRISPR/Cas9 PAM Constraint for Single-Nucleotide Resolution Interventions

(2016) Yi Li et al.   PLoS One

CRISPR/Cas9-mediated gene knockout in the mouse brain using in utero electroporation

(2016) Yohei Shinmyo et al.   Scientific Reports

Sequence features associated with the cleavage efficiency of CRISPR/Cas9 system

(2016) Xiaoxi Liu et al.   Scientific Reports

CRISPR/Cas9-mediated conversion of human platelet alloantigen allotypes

(2015) N. Zhang et al.   BLOOD

Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds

(2015) Jesse G. Zalatan et al.   CELL

Crystal Structure of Staphylococcus aureus Cas9

(2015) Hiroshi Nishimasu et al.   CELL

CRISPR/Cas9-mediatedDax1knockout in the monkey recapitulates human AHC-HH

(2015) Yu Kang et al.   HUMAN MOLECULAR GENETICS

CRISPR-Cas9 for medical genetic screens: applications and future perspectives

(2015) Hui-Ying Xue et al.   JOURNAL OF MEDICAL GENETICS

Engineered CRISPR-Cas9 nucleases with altered PAM specificities

(2015) Benjamin P. Kleinstiver et al.   NATURE

In vivo genome editing using Staphylococcus aureus Cas9

(2015) F. Ann Ran et al.   NATURE

Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition

(2015) Benjamin P Kleinstiver et al.   NATURE BIOTECHNOLOGY

A light-inducible CRISPR-Cas9 system for control of endogenous gene activation

(2015) Lauren R Polstein et al.   Nature Chemical Biology

Beyond editing: repurposing CRISPR–Cas9 for precision genome regulation and interrogation

(2015) Antonia A. Dominguez et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

A Cas9-guide RNA complex preorganized for target DNA recognition

(2015) F. Jiang et al.   SCIENCE

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

PAM multiplicity marks genomic target sites as inhibitory to CRISPR-Cas9 editing

(2015) Abba Malina et al.   Nature Communications

A quick guide to CRISPR sgRNA design tools

(2015) Vincent A Brazelton et al.   GM Crops & Food-Biotechnology in Agriculture and the Food Chain

Improving the specificity and efficacy of CRISPR/CAS9 and gRNA through target specific DNA reporter

(2014) Jian-Hua Zhang et al.   JOURNAL OF BIOTECHNOLOGY

Efficient Mutagenesis by Cas9 Protein-Mediated Oligonucleotide Insertion and Large-Scale Assessment of Single-Guide RNAs

(2014) James A. Gagnon et al.   PLoS One

Surrogate reporter-based enrichment of cells containing RNA-guided Cas9 nuclease-induced mutations

(2014) Suresh Ramakrishna et al.   Nature Communications

High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity

(2013) Vikram Pattanayak et al.   NATURE BIOTECHNOLOGY

ConSurf 2010: calculating evolutionary conservation in sequence and structure of proteins and nucleic acids

(2010) H. Ashkenazy et al.   NUCLEIC ACIDS RESEARCH

Phylogeny.fr: robust phylogenetic analysis for the non-specialist

(2008) A. Dereeper et al.   NUCLEIC ACIDS RESEARCH