Review
Food Science & Technology
Meng-ge Tang, Sheng Zhang, Li-gui Xiong, Jing-hui Zhou, Jian-an Huang, Ai-qing Zhao, Zhong-hua Liu, Ai-ling Liu
Summary: Polyphenol oxidase (PPO) is a metalloenzyme in tea plants that plays a crucial role in enhancing defensive responses, cultivating high-quality germplasm resources, and producing functional tea products. The oxidative polymerization of PPO in tea manufacturing can provide functional foods for preventing and treating chronic non-communicable diseases. However, more comprehensive research is needed to understand the relationship between PPO and tea. This article provides a comprehensive review of the classification, catalytic mechanism, and utilization of PPO in modulating tea flavors, compositions, and nutrition, as well as the techniques for modifying and applying PPO based on modern enzymology and synthetic biology.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2023)
Article
Food Science & Technology
Sara Serra, Brendon Anthony, Francesca Boscolo Sesillo, Andrea Masia, Stefano Musacchi
Summary: Phenolic compounds in fruit have health benefits and affect post-harvest fruit quality. Enzymatic activity and phenolic concentration play key roles in fruit browning, with different apple cultivars showing varying degrees of browning susceptibility.
Article
Chemistry, Applied
Marta Maria Calvo, Aikaterini Tzamourani, Oscar Martinez-Alvarez
Summary: The study found that the polyphenol extracts from these halophyte plants inhibited diphenol oxidase activity more efficiently than sodium sulfite. The purslane extract, rich in isoorientins, isovitexin, and apigenin, showed the highest inhibiting effect, classified as mixed or non-competitive. These polyphenol extracts could be promising melanosis-inhibiting agents for seafood industries.
Article
Food Science & Technology
Jiang-Feng Yuan, Zeng-Chao Hou, Da-Hong Wang, Zhi-Jun Qiu, Ming-Gui Gong, Jian-Rui Sun
Summary: This study investigated the changes in PPO activities and the possible passivation mechanism during the maceration stage of winemaking at different microwave conditions. The results showed that microwave irradiation can affect the activity of PPO by inducing carbon-centred free radicals and influencing PPO's composition, microenvironment, groups, chemical bonds, structure, and hydrolysis degree. This research will contribute to understanding the mechanism of higher extraction rate of phenols compounds in winemaking maceration stage by microwave irradiation.
Article
Food Science & Technology
Shuang Jia, Shu Jiang, Yi Chen, Yingying Wei, Xingfeng Shao
Summary: This study focused on the inhibitory effects of different substances on soluble polyphenol oxidase (sPPO) and membrane-bound polyphenol oxidase (mPPO) in peach fruit. Ascorbic acid showed the highest inhibitory effect on both sPPO and mPPO, while beta-cyclodextrin only had a direct inhibitory effect on sPPO. The results of this study provide a basis for preventing browning in peach fruit.
Article
Acoustics
Jing Yang, Nana Li, Chunyu Wang, Tao Chang, Huichuan Jiang
Summary: This study focused on the extraction process of polyphenols from coconut mesocarp, comparing different extraction methods and optimizing parameters to achieve maximum total flavonoid and phenolic content. The results showed that ultrasound-assisted extraction and homogenization-assisted extraction were the most efficient methods, with a combination of both further enhancing extraction efficiency. The study provides a technical guarantee for the further utilization of phenolic substances in coconut mesocarp.
ULTRASONICS SONOCHEMISTRY
(2021)
Article
Chemistry, Applied
Bin Feng, Sibo Zhang, Di Wang, Yalong Li, Pai Zheng, Long Gao, Da Huo, Lei Cheng, Shuangying Wei
Summary: In this study, a soy protein isolate nano-silver hydrosol was prepared using an environmentally friendly method, and a polyacrylate-nano silver antibacterial wood coating with excellent antibacterial properties was obtained.
PROGRESS IN ORGANIC COATINGS
(2022)
Article
Multidisciplinary Sciences
Xiaohui Liu, Aidong Zhang, Jing Shang, Zongwen Zhu, Ye Li, Xuexia Wu, Dingshi Zha
Summary: This study investigated the browning mechanism in fresh-cut eggplant using an untargeted metabolomics approach and physiology experiments. It was found that the content of chlorogenic acid may play a significant role in browning, and the activities of enzymes related to phenylpropanoid metabolism increased with prolonged cutting time. Additionally, the accumulation of ROS and changes in antioxidant enzyme activities were observed after cutting, providing insights into the browning process during fruit and vegetable processing.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Inorganic & Nuclear
Karuppiah Chandran Priyadarshni, Raman Krishnamoorthi, Chelladurai Mumtha, Pambayan Ulagan Mahalingam
Summary: This study synthesized eco-friendly and non-toxic silver nanoparticles (AgNPs) using the extract of oyster mushroom, P. florida, and demonstrated their significant antimicrobial activity. The nutritional profile of P. florida dried mushroom revealed high protein and vitamin content. The synthesized AgNPs were characterized by various bio-physical techniques and showed pure crystalline form with a small particle size. The synergistic effects of mushroom extract and AgNPs were effective in controlling diseases caused by microbial infections.
INORGANIC CHEMISTRY COMMUNICATIONS
(2022)
Article
Plant Sciences
Shujun Wang, Guo Wang, Huanling Li, Fang Li, Jiabao Wang
Summary: This study established an efficient and stable genetic transformation technology in litchi and successfully edited genes in litchi using the CRISPR/Cas9 system. It provides a foundation for investigating gene functions and accelerating litchi genetic improvement.
HORTICULTURAL PLANT JOURNAL
(2023)
Article
Chemistry, Applied
Xuezhi Tian, Lei Rao, Liang Zhao, Yongtao Wang, Xiaojun Liao
Summary: In this study, it was found that EGCG could inhibit PPO activity by competing for catalytic centers and inducing aggregation. The binding mode of EGCG with PPO and the conformational and secondary structure changes of PPO after binding were revealed through various experimental methods and computational simulations.
Article
Plant Sciences
Fei Qin, Chunhua Hu, Tongxin Dou, Ou Sheng, Qiaosong Yang, Guiming Deng, Weidi He, Huijun Gao, Chunyu Li, Tao Dong, Ganjun Yi, Fangcheng Bi
Summary: This study analyzed the PPO gene family in bananas and identified MaPPO1 and MaPPO6 as the main genes responsible for fruit browning. These findings provide a foundation for the development of banana varieties with low fruit browning.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Peyman Ebrahimi, Carlo Nicoletto, Paolo Sambo, Federica Tinello, Dasha Mihaylova, Anna Lante
Summary: This study evaluated the effect of fertilization techniques and grafting on polyphenol oxidase (PPO) activity and colorimetric parameters in cultivated eggplants. Fertilization alone significantly increased PPO activity, while fertilization combined with grafting decreased PPO activity. There was a significant positive correlation between PPO activity and the a* values of the eggplants. Additionally, eggplant slices exposed to room temperature for 60 minutes showed a significant increase in PPO activity and color parameters.
Article
Engineering, Environmental
Azam Marjani, Masoud Habibi Zare, Mohammad Hasan Sadeghi, Saeed Shirazian, Mahdi Ghadiri
Summary: The newly developed CPPE-alginate nanocatalyst shows high efficiency and stability in phenol removal from wastewater, making it a promising option for hazardous wastewater treatment.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Chemistry, Applied
Tao Liao, Lei Zhou, Junping Liu, Liqiang Zou, Taotao Dai, Wei Liu
Summary: This study found that salicylic acid plays an important role in inhibiting PPO through acidification and binding effects. As buffer concentration increased, the acidification effect decreased while the binding effect became more predominant. Additionally, salicylic acid competitively inhibited PPO, inducing changes in secondary structure.
Review
Environmental Sciences
Sikandar I. Mulla, Zabin K. Bagewadi, Basheerabegum Faniband, Muhammad Bilal, Jong-Chan Chae, Paul Olusegun Bankole, Ganesh Dattatraya Saratale, Ram Naresh Bhargava, Dummi Mahadevan Gurumurthy
Summary: Pharmaceutical active drug, particularly sulfamethazine (SMZ), is considered as a major emerging microcontaminant that can affect the development of antibacterial resistance genes due to its long-term presence in the environment. The widespread use of SMZ and its detection in various samples, including water, along with its toxic effects on microalgae and mice, has raised concerns. Therefore, further research is needed to investigate the removal and degradation of SMZ in wastewater treatment plants, including the use of engineered biological mediators.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Kotresh Kyathanahally Rajashekarappa, Gurumurthy Dummi Mahadevan, Shivayogeeswar Eshwarappa Neelagund, Madhuri Sathynarayana, Divya Vijaya, Sikandar Mulla
Summary: The study investigated microbial decolorization of azo dyes in industrial wastewater, with results showing improved efficiency with specific carbon and nitrogen sources. Geobacillus thermoleovorans KNG 112 was found to be effective at decolorizing the dyes at specific temperatures and pH levels. Fourier transform infrared spectral analysis revealed the formation of different organic compounds during the decolorization process. The findings suggest potential applications of G. thermoleovorans KNG 112 in treating industrial wastewater contaminated with azo dyes.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2022)
Review
Microbiology
Prabhakar Mishra, N. S. Kiran, Luiz Fernando Romanholo Ferreira, Sikandar I. Mulla
Summary: The increasing demand for cosmetics has led to extensive industrial production, with organic and inorganic chemicals causing ecological concerns. Microbial remediation, particularly involving algae, shows promising potential for addressing persistent cosmetic chemical pollutants and promoting environmental sustainability.
JOURNAL OF BASIC MICROBIOLOGY
(2022)
Article
Biophysics
Paul Olusegun Bankole, Victor Taghoghor Omoni, Chidinma Angela Tennison-Omovoh, Seun Owolabi Adebajo, Sikandar Imamsab Mulla, Adedotun Adeyinka Adekunle, Kirk Taylor Semple
Summary: The promising potentials of biocatalytic treatment processes in the removal of micropollutants have attracted great attention. This study compared the efficiency of a novel laccase from Xylaria polymorpha with commercial laccases from Trametes versicolor and Aspergillus sp. The results showed that XPL exhibited better oxidation performance on AMX. Optimization of operational conditions revealed the best oxidation conditions, and violuric acid was determined as the best redox mediator. Two metabolic products of AMX were identified. The toxicity assessments showed significantly reduced toxicity after oxidation. This study further demonstrated the efficiency of biocatalytic-mediator technology in transforming complex micropollutants into less toxic substances in an eco-friendly way.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Chemistry, Inorganic & Nuclear
Sreekanth Rajagopalachar, Jayadev Pattar, Sikandar Mulla
Summary: In this work, high surface area MgO nanoparticles were successfully synthesized without using any directing agent or capping agent. The experimental conditions required to achieve high surface area MgO nanoparticles were investigated. The optical, structural, and morphological properties of the MgO nanoparticles were characterized using various techniques. The MgO nanoparticles exhibited good antibacterial activity against different bacteria.
INORGANIC CHEMISTRY COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Paul Olusegun Bankole, Victor Taghoghor Omoni, Sikandar Imamsab Mulla, Seun Owolabi Adebajo, Adedotun Adeyinka Adekunle
Summary: The present study investigated the potential of marine-derived fungal co-culture in the mycoremediation of polycyclic aromatic hydrocarbons (PAHs). The results demonstrated the efficiency of the co-culture in degrading fluoranthene, a PAH, with the induction of various enzymes. The analysis of metabolites provided insights into the metabolic pathway. These findings highlight the significance of marine-derived fungal co-biomass in the eco-friendly remediation of PAHs.
ARABIAN JOURNAL OF CHEMISTRY
(2022)
Article
Environmental Sciences
Christina Saran, Diane Purchase, Ganesh Dattatraya Saratale, Rijuta Ganesh Saratale, Luiz Fernando Romanholo Ferreira, Muhammad Bilal, Hafiz M. N. Iqbal, Chaudhery Mustansar Hussain, Sikandar Mulla, Ram Naresh Bharagava
Summary: This review paper discusses the origin of hexavalent chromium toxicity in tannery wastewater and its remediation using Microbial Fuel Cell (MFC) technology. The paper highlights the role of electroactive bacteria in treating wastewater, producing bioenergy, and reducing Cr6+. Various parameters such as electrode, pH, operation mode, operation time, and exchange membrane type are discussed to enhance MFC production and remediation of Cr6+. The paper also emphasizes the importance of MFC in reducing hexavalent chromium contamination and generating energy economically.
Article
Biochemistry & Molecular Biology
Zabin K. Bagewadi, Venessa Dsouza, Deepak A. Yaraguppi, Sikandar I. Mulla, Sanjay H. Deshpande, Shreya S. Shettar
Summary: The investigation focuses on the enhanced production of bacterial cellulose (BC) from Enterobacter hormaechei subsp. steigerwaltii strain ZKE7 using different raw sources. Optimization achieved a 7.54-fold increase in BC production, reaching 31.7 g/L BC (In-situ). Composites made from the optimized BC demonstrated excellent water retention capability, moisture content, water release rate, porosity, thickness, and protein adsorption.
PROCESS BIOCHEMISTRY
(2023)
Review
Environmental Sciences
Shoumi Haldar, Yuvashree Muralidaran, Diana Miguez, Sikandar I. Mulla, Prabhakar Mishra
Summary: This review paper discusses the source, bio-accumulation, potential toxicity impact, and management strategies of nanoplastics pollution. It shows that nanoplastics interfere with metabolic pathways, causing organ dysfunction and altering organism physiology and behavior. The major exposure route for nanoplastics is ingestion, although inhalation is also possible. However, there is limited research on the risk assessment of nanoplastics toxicity for human health, highlighting the importance of studying their potential impacts and environmental accumulation.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Review
Energy & Fuels
Noureddine El Messaoudi, Mohammed El Khomri, Abdelaziz El Mouden, Amal Bouich, Amane Jada, Abdellah Lacherai, Hafiz M. N. Iqbal, Sikandar I. Mulla, Vineet Kumar, Juliana Heloisa Pine Americo-Pinheiro
Summary: The rapid growth of the global economy has resulted in severe anthropogenic pollution, particularly in the aquatic systems. Traditional treatment methods create new forms of pollution, necessitating the need for innovative regeneration techniques for polluted adsorbents. Low-cost adsorbents can be regenerated using different desorbing agents and have high potential for reusability.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Review
Plant Sciences
Larissa Renata Santos Andrade, Raul Jose Alves Felisardo, Ianny Andrade Cruz, Muhammad Bilal, Hafiz M. N. Iqbal, Sikandar I. I. Mulla, Ram Naresh Bharagava, Ranyere Lucena de Souza, Lucas Carvalho Basilio Azevedo, Luiz Fernando Romanholo Ferreira
Summary: Cassava processing generates a large amount of waste, but this waste has excellent potential for bioenergy and non-energy products. However, the sustainability of biorefineries faces environmental challenges and uncertainties. Life cycle assessment provides information to help address production bottlenecks and improve competitiveness.
Review
Environmental Sciences
Prabhakar Mishra, Neelakanta Sarvashiva Kiran, Luiz Fernando Romanholo Ferreira, Krishna Kumar Yadav, Sikandar I. Mulla
Summary: Petroleum products are essential for energy and are widely used in various industries and everyday life. However, their runoff can cause carbonaceous contamination in both marine and terrestrial environments. The adverse effects of petroleum hydrocarbons on human health and the environment highlight the need for remedial strategies, such as bioremediation, to eliminate these pollutants.
Article
Environmental Sciences
Luciano de Melo, Ianny Andrade Cruz, Lucas Tadeu Fuess, Carlos Eduardo Maynard Santana, Ram Naresh Bharagava, Sikandar I. Mulla, Muhammad Bilal, Ganesh Dattatraya Saratale, Renan Tavares Figueiredo, Luiz Fernando Romanholo Ferreira
Summary: This study presents a technological solution called biogas analyzer module (BAM) based on the Arduino platform, which can track the profile of produced biogas. The lab-made BAM showed good accuracy and efficiency, as well as cost reduction, with a potential six-fold cheaper price compared to existing market devices, when compared with established standards (chromatographic and colorimetric methods).
ENVIRONMENTAL TECHNOLOGY
(2023)
Article
Environmental Sciences
Atif Khurshid Wani, Nahid Akhtar, Nafiaah Naqash, Farida Rahayu, Djajadi Djajadi, Chirag Chopra, Reena Singh, Sikandar I. Mulla, Farooq Sher, Heloisa Pine Americo-Pinheiro Juliana
Summary: Microplastics (MPs) are pervasive pollutants in the environment, with increasing accumulation in aquatic, marine, and terrestrial ecosystems due to human activities. These MPs, which degrade slowly, pose a threat to biota by increasing the probability of biomagnification of persistent, bioaccumulative, and toxic substances. In order to remediate MP-pollutants, efficient strategies are needed to prevent the leaching of contaminants into water, soil, and eventually into humans. Microorganisms capable of degrading plastic polymers through enzymatic actions have been discovered, and metagenomics is an effective tool for exploring and characterizing these plastic-degrading microbial consortia and enzymes. Combined with metatranscriptomics and metabolomics, metagenomics can identify and select remediation-efficient microbes in their natural habitats. Advances in bioinformatics and sequencing tools enable rapid screening, mining, and prediction of genes involved in polymer degradation. This review provides a comprehensive summary of the growing threat of microplastics globally and emphasizes the role of metagenomics and computational biology in developing effective strategies for MP remediation.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)