Article
Biochemical Research Methods
Judith Fischer, Mascha Treblin, Tobias Sitz, Sascha Rohn
Summary: The use of algae and cyanobacteria in human nutrition is becoming increasingly important, with health effects believed to be influenced by minor components such as sulfolipids. Studies have found compounds like sulfoquinovosyldiacylglycerols (SQDGs) to play a role in the health effects of algae and cyanobacteria.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Horticulture
Christoph Hahn, Anja Mueller, Nikolai Kuhnert, Dirk C. Albach
Summary: Kale, a member of the Brassica oleracea L. crops, has gained global recognition for its high nutritional value and versatility. The diversity of different kale varieties and the effect of chilling on glucosinolate diversity and taste have been explored. This study found different patterns of glucosinolate change in different kale varieties after cold acclimation, suggesting that genetic and environmental factors both play a role in the composition of glucosinolate patterns in kale.
Article
Chemistry, Analytical
Jia-Ning Gao, Ye Li, Jun Liang, Jun-Hong Chai, Hai-Xue Kuang, Yong-Gang Xia
Summary: A direct acetylation strategy combined with RPLC-ESI-MRM-MS was developed for simultaneous determination of multiple carbohydrates. The method was successfully applied for compositional analysis of complex polysaccharides.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Plant Sciences
Shihang Sun, Jinbao Fang, Miaomiao Lin, Chungen Hu, Xiujuan Qi, Jinyong Chen, Yunpeng Zhong, Abid Muhammad, Zhi Li, Yukuo Li
Summary: Metabolome and transcriptome profiles of freezing-tolerant and freezing-sensitive kiwifruit genotypes were studied under cold stress, revealing specific upregulation of flavonoid metabolic pathways in the tolerant genotype KL and nucleotides metabolism pathways in the sensitive genotype RB. Key metabolism components were found to participate in the regulation of freezing tolerance in both genotypes.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemical Research Methods
Kin-Wing Lui, Sai-Ming Ngai
Summary: The heterogeneity of histone H3 proteoforms poses a challenge for top-down analysis, but using optimized EThcD parameters, the study found that both dual-gradient WCX/HILIC and organic-rich WCX/HILIC methods can separate different trimethyl/acetyl near-isobaric H3 proteoforms. Compared to RPLC-MS, both WCX/HILIC-MS methods improved the quality of H3 PrSMs and significantly enhanced the identification range, reproducibility, and confidence in H3 proteoforms.
JOURNAL OF PROTEOME RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Yuting Sun, Huiru Tang, Yulan Wang
Summary: Carbonyl-containing metabolites are important and challenging to quantify directly using RPLC-ESI-MS due to poor ionization efficiency. The stable isotope-coded derivatization (ICD) strategy overcomes this challenge, allowing for accurate quantification and submetabolome analysis in LC-MS methods.
Article
Chemistry, Applied
Maher Mahmoudi, Raoudha Abdellaoui, Faycal Boughalleb, Boutheina Yahia, Mahmoud Mabrouk, Nizar Nasri
Summary: This study investigated the lipid, protein, and bioactive compound contents in the seeds of Astragalus armatus, A. caprinus, and A. gombiformis, finding that they could be valuable assets for functional foods and/or food ingredients.
Article
Food Science & Technology
Paolo Bellassi, Gabriele Rocchetti, Gianluca Maldarizzi, Gian Paolo Braceschi, Lorenzo Morelli, Luigi Lucini, Fabrizio Cappa
Summary: This study investigated the microbiological quality and chemical profiles of two different dairy creams obtained by centrifugation vs. natural creaming separation systems. Metabolomics revealed significant differences in triacylglycerols and phospholipids accumulation trends between the two creaming methods. Multivariate statistical analysis identified potential marker compounds in amino acids and lipids for discriminating ricotta cheese during its shelf-life period.
Article
Chemistry, Analytical
ShaoJing Liu, Sen Guo, YuFei Hou, ShanShan Zhang, Lu Bai, ChiTang Ho, LiLi Yu, Lin Yao, BingNan Zhao, NaiSheng Bai
Summary: This study investigated the chemical composition differences of 35 oil peony leaves from four provinces in China using methods such as high-performance liquid chromatography (HPLC). Seventeen common characteristic peaks were identified, and the similarity of the samples ranged from 0.864 to 0.999. Multivariate analysis divided the samples into four groups. Ten marker components closely related to quality evaluation were determined. This study provides a reasonable method for the discrimination and quality evaluation of oil peony leaves from different producing areas.
MICROCHEMICAL JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Milena Masullo, Antonietta Cerulli, Cosimo Pizza, Sonia Piacente
Summary: This study analyzed the specialized metabolites of Pouteria lucuma fruit pulp and skin, identifying phenolic, flavonoid, and polar lipid compounds. The study also evaluated the phenolic content and antioxidant activity of the extracts and isolated compounds from the fruit.
Article
Biochemistry & Molecular Biology
Julio Cesar Polonio, Marcos Alessandro dos Santos Ribeiro, Cintia Zani Favaro-Polonio, Eduardo Cesar Meurer, Joao Lucio Azevedo, Halison Correia Golias, Joao Alencar Pamphile
Summary: Endophytic microorganisms have the potential for biotechnological exploitation due to their ability to produce secondary compounds involved in endophyte-plant adaptation and their interactions with other living organisms. This study evaluates the effect of pH on the extraction process of secondary metabolites from the Diaporthe citri strain G-01 endophyte. The pH of the fermented broth was found to influence the chemical profile and presence of important ions.
Article
Environmental Sciences
Rofida Wahman, Catarina Cruzeiro, Johanna Grassmann, Peter Schroeder, Thomas Letzel
Summary: Metabolomics was used to investigate metabolic changes in Lemna minor exposed to different concentrations of diclofenac sodium, revealing that DCF may increase antioxidant responses and alter physiological characteristics in Lemna. Similar metabolic responses have been found in other studies exposing Lemna to anthropogenic stressors.
Article
Food Science & Technology
Felipe Nardo dos Santos, Estefania Julia Dierings de Souza, Tatiane Jessica Siebeneichler, Juliani Buchveitz Pires, Dianini Huttner Kringel, Adriana Dillenburg Meinhart, Alvaro Renato Guerra Dias, Elessandra da Rosa Zavareze
Summary: Jambolan fruit extract, rich in anthocyanins, showed strong antioxidant activity and a wide color variation at different pH values. The optimized extraction conditions resulted in a extract containing a high concentration of anthocyanins, with Del3,5D being the major compound. These findings suggest that jambolan fruit extract has potential as a natural substitute for synthetic dyes and for developing pH-sensitive smart packaging.
FOOD ANALYTICAL METHODS
(2022)
Article
Genetics & Heredity
Johny Ijaq, Deepika Chandra, Malay Kumar Ray, M. V. Jagannadham
Summary: The study successfully identified the functional roles of hypothetical proteins in cold adaptation of Pseudomonas sp. Lz4W through proteome analysis and bioinformatics tools, providing insights into biological mechanisms at the molecular level.
FRONTIERS IN GENETICS
(2022)
Article
Chemistry, Applied
Chen He, Jun Cao, Yuyan Bao, Zexin Sun, Zhongyuan Liu, Chuan Li
Summary: A comprehensive lipidomic evaluation was conducted on tilapia muscle, head, and viscera, revealing higher lipid content in head and viscera compared to muscle. The study showed that polyunsaturated fatty acids had higher percentages in phospholipids than in triacylglycerols, with C52:2 and C52:3 identified as the most abundant triacylglycerols. Ultra-performance liquid chromatography-quadrupole time-of-flight-mass spectrometry identified 622, 530, and 513 lipids in the muscle, head, and viscera respectively, with five fatty acids and 33 lipid species considered as potential biomarkers.
Article
Food Science & Technology
Salvatore Multari, Concetta Licciardello, Marco Caruso, Andrea Anesi, Stefan Martens
Summary: Citrus peels, specifically the albedo, have been shown to have superior physicochemical properties compared to the flavedo. Enzyme-assisted extraction with pectinase and cellulase significantly enhanced the recovery of phenolic compounds from both flavedo and albedo, with the latter demonstrating higher yields. These results suggest that albedo could be utilized as a novel raw material in the food industry, and enzyme-assisted extraction is an effective method to recover phenolics from citrus peels.
JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION
(2021)
Article
Biochemistry & Molecular Biology
Susanna Fiorelli, Andrea Anesi, Benedetta Porro, Nicola Cosentino, Jose P. Werba, Alessandro Di Minno, Chiara Maria Manega, Simone Barbieri, Gualtiero Colombo, Giancarlo Marenzi, Viviana Cavalca, Elena Tremoli, Sonia Eligini
Summary: The study focused on investigating the migration capacity of monocytes isolated from patients with acute myocardial infarction (AMI) at hospital presentation and its correlation with cellular lipid profile. It was found that AMI patients' monocytes exhibited increased migration and a distinct lipidomic profile compared to controls, with lactosylceramide levels positively correlating with migration and lactosylceramide synthase inhibition significantly reducing monocyte migration induced by fetal bovine serum. These results suggest a potential mechanism involving lactosylceramide in the development of atherothrombosis and AMI.
Article
Marine & Freshwater Biology
Ulrike Obertegger
Summary: Under-ice community dynamics are influenced by seasonal and spatial factors, with light availability and primary production playing important roles. Future changes in ice conditions with less snow cover may lead to bacterial communities resembling those observed in high-light years.
Article
Endocrinology & Metabolism
Ines Delgado, Sofia Cussotto, Andrea Anesi, Sandra Dexpert, Agnes Aubert, Bruno Aouizerate, Cedric Beau, Damien Forestier, Patrick Ledaguenel, Eric Magne, Fulvio Mattivi, Lucile Capuron
Summary: This study found that chronic low-grade inflammation in obesity played an important role in depressive symptoms. One mechanism involved the effects of inflammation on tryptophan metabolism. High levels of high-sensitive C-reactive protein and low levels of indole-3-carboxaldehyde were associated with more severe depressive symptoms.
INTERNATIONAL JOURNAL OF OBESITY
(2022)
Article
Endocrinology & Metabolism
Ximena Orozco-Ruiz, Andrea Anesi, Fulvio Mattivi, Monique M. B. Breteler
Summary: Visceral and subcutaneous adipose tissue function as endocrine organs and affect metabolic health through metabolite concentrations. This study found that certain metabolite concentrations associated with cardiometabolic risk markers are altered with increased visceral adipose tissue volume.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Biochemical Research Methods
Andrea Anesi, Kirsten Berding, Gerard Clarke, Catherine Stanton, John F. Cryan, Noel Caplice, R. Paul Ross, Andrea Doolan, Urska Vrhovsek, Fulvio Mattivi
Summary: The modulation of host and dietary metabolites by gut microbiota is crucial for maintaining host physiology and understanding various pathologies. A new chromatography-mass spectrometry method was developed to quantitate 89 metabolites resulting from human-gut microbial cometabolism in plasma, serum, and urine. This method provides accurate and efficient measurement of metabolite concentrations in biological fluids, making it a valuable tool for studying changes in human-gut microbial cometabolism in health and disease.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Marine & Freshwater Biology
Ulrike Obertegger, Giovanna Flaim, Stefano Corradini, Leonardo Cerasino, Tamar Zohary
Summary: Little is known about the dynamics of under-ice phytoplankton communities. A 6-year study in Lake Tovel, Italy, revealed differences in phytoplankton community composition between summer and under-ice seasons. Bacillariophyta dominated in summer, while Miozoa (class Dinophyceae) dominated under ice. Shifts in size classes were observed in Bacillariophyta, Chlorophyta, and Ochrophyta during the transition from summer to under ice, with the opposite pattern observed in Dinophyceae. No strong correlations were found between phytoplankton community composition and abiotic factors. It is suggested that inter-species relationships and more precise indicators of under-ice light should be considered for a better understanding of under-ice processes.
Article
Zoology
Leonid Svetlichny, Johann Rudi Strickler, Ulrike Obertegger
Summary: Cyclopoid and calanoid copepods have different ways of movement, with the small cyclopoid copepod showing faster escape swimming speed and requiring higher mechanical energy in jumping mode compared to the calanoid copepod.
JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL AND INTEGRATIVE PHYSIOLOGY
(2022)
Article
Biology
Leonid Svetlichny, Ulrike Obertegger
Summary: Little is known about the swimming activity and respiration rate of the cyclopoid copepod Cyclops vicinus. This study measured the swimming speed and respiration rate of C. vicinus at different temperatures and found log-linear relationships between temperature and duration, speed, and frequency of locomotor acts. The respiration rate showed log-linear relationships with temperature and a thermal coefficient Q10 > 2 was found. The energy efficiency of copepod swimming was also investigated, and linear relationships between swimming speed and mechanical and metabolic energy were found. Cold-water specialization may limit the increase of swimming speed in C. vicinus at high temperatures compared to warm-water adapted species.
JOURNAL OF THERMAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Kirsten Berding, Thomaz F. S. Bastiaanssen, Gerard M. Moloney, Serena Boscaini, Conall R. Strain, Andrea Anesi, Caitriona Long-Smith, Fulvio Mattivi, Catherine Stanton, Gerard Clarke, Timothy G. Dinan, John F. Cryan
Summary: This study investigated the impact of a psychobiotic diet on the microbial profile and mental health in a healthy population. The results showed that the psychobiotic diet reduced perceived stress and improved stress levels, although it only had subtle effects on microbial composition and function. In addition, higher adherence to the diet was associated with greater decreases in perceived stress.
MOLECULAR PSYCHIATRY
(2023)
Article
Multidisciplinary Sciences
Gesa A. Weyhenmeyer, Ulrike Obertegger, Hugo Rudebeck, Ellinor Jakobsson, Joachim Jansen, Galina Zdorovennova, Sheel Bansal, Benjamin D. Block, Cayelan C. Carey, Jonathan P. Doubek, Hilary Dugan, Oxana Erina, Irina Fedorova, Janet M. Fischer, Laura Grinberga, Hans-Peter Grossart, Kulli Kangur, Lesley B. Knoll, Alo Laas, Fabio Lepori, Jacob Meier, Nikolai Palshin, Mark Peternell, Merja Pulkkanen, James A. Rusak, Sapna Sharma, Danielle Wain, Roman Zdorovennov
Summary: The quality and characteristics of lake ice play a crucial role in ice safety and the ecology beneath the ice. This study conducted a sampling campaign across the Northern Hemisphere during a warm winter and found that the lake ice commonly consists of unstable white ice, which increases in thickness towards the end of the ice cover season. The dominance of white ice is attributed to air temperatures fluctuating around the freezing point, which occurs more frequently during warmer winters. Under continued global warming, lakes will be increasingly covered by white ice, affecting ice safety and the growth of primary producers.
NATURE COMMUNICATIONS
(2022)
Article
Limnology
Vittoria Ferrari, Arianna Gualdi, Isabella Bertani, Diego Fontaneto, Lyudmila Kamburska, Karimullah Karimullah, Federico Marrone, Ulrike Obertegger, Giampaolo Rossetti, Rocco Tiberti, Tommaso Cancellario
Summary: We present a dataset containing known occurrence records of Italian Rotifera taxa at species and subspecies levels. The dataset comprises 15,525 records from 584 valid species and subspecies names, collected from 332 published papers spanning from 1838 to 2022. The dataset, including georeferenced occurrence data, is available through the Open Science Framework (OSF) and Global Biodiversity Information Facility (GBIF).
JOURNAL OF LIMNOLOGY
(2023)
Review
Environmental Sciences
Ulrike Obertegger, Robert Lee Wallace
Summary: Trait-based research on plankton has gained interest due to its potential role in ecological studies. However, research on rotifer traits has been lacking compared to other plankton groups. In this study, we reviewed the literature and developed a data matrix for rotifer traits, including seven key characteristics. We faced challenges in assigning traits, particularly in assessing feeding traits due to limited information. Our trait matrix will provide a foundation for future research on rotifer functional diversity and improve classification accuracy.
Review
Limnology
Kaitlin L. Reinl, Ted D. Harris, Rebecca L. North, Pablo Almela, Stella A. Berger, Mina Bizic, Sarah H. Burnet, Hans-Peter Grossart, Bastiaan W. Ibelings, Ellinor Jakobsson, Lesley B. Knoll, Brenda M. Lafrancois, Yvonne McElarney, Ana M. Morales-Williams, Ulrike Obertegger, Igor Ogashawara, Ma Cristina Paule-Mercado, Benjamin L. Peierls, James A. Rusak, Siddhartha Sarkar, Sapna Sharma, Jessica V. Trout-Haney, Pablo Urrutia-Cordero, Jason J. Venkiteswaran, Danielle J. Wain, Katelynn Warner, Gesa A. Weyhenmeyer, Kiyoko Yokota
Summary: Cyanobacterial blooms have negative impacts on freshwater ecosystems, including releasing toxins, blocking light, and depleting oxygen. Climate change may promote cyanobacterial blooms, as increasing lake surface temperature can enhance their growth. Recent evidence shows that cyanobacterial blooms can also occur in cold-water temperatures (< 15 degrees C), even under ice-covered conditions.
LIMNOLOGY AND OCEANOGRAPHY LETTERS
(2023)
Article
Neurosciences
Kolade Oluwagbemigun, Andrea Anesi, Gerard Clarke, Matthias Schmid, Fulvio Mattivi, Ute Nothlings
Summary: The study found 5 strong and temporally reproducible associations among tryptophan and its metabolites in healthy adolescents, revealing new metabolic pathways.
INTERNATIONAL JOURNAL OF TRYPTOPHAN RESEARCH
(2021)
