Measuring the sequence-affinity landscape of antibodies with massively parallel titration curves

Determination of binding affinity upon mutation for type I dockerin-cohesin complexes fromClostridium thermocellumandClostridium cellulolyticumusing deep sequencing

(2016) Caitlin A. Kowalsky et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Rapid Fine Conformational Epitope Mapping Using Comprehensive Mutagenesis and Deep Sequencing

(2015) Caitlin A. Kowalsky et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Conformation-Dependent Epitopes Recognized by Prion Protein Antibodies Probed Using Mutational Scanning and Deep Sequencing

(2015) Kyle M. Doolan et al.   JOURNAL OF MOLECULAR BIOLOGY

SORTCERY—A High–Throughput Method to Affinity Rank Peptide Ligands

(2015) Lothar “Luther” Reich et al.   JOURNAL OF MOLECULAR BIOLOGY

Precise and Efficient Antibody Epitope Determination through Library Design, Yeast Display and Next-Generation Sequencing

(2015) Thomas Van Blarcom et al.   JOURNAL OF MOLECULAR BIOLOGY

Inferring processes underlying B-cell repertoire diversity

(2015) Yuval Elhanati et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Directed Evolution of Brain-Derived Neurotrophic Factor for Improved Folding and Expression in Saccharomyces cerevisiae

(2014) Michael L. Burns et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Tracking global changes induced in the CD4 T-cell receptor repertoire by immunization with a complex antigen using short stretches of CDR3 protein sequence

(2014) Niclas Thomas et al.   BIOINFORMATICS

T-cell receptor repertoires share a restricted set of public and abundant CDR3 sequences that are associated with self-related immunity

(2014) Asaf Madi et al.   GENOME RESEARCH

Deep mutational scanning: a new style of protein science

(2014) Douglas M Fowler et al.   NATURE METHODS

Quantifying selection in immune receptor repertoires

(2014) Yuval Elhanati et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

B-cell repertoire responses to varicella-zoster vaccination in human identical twins

(2014) Chen Wang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Distinctive properties of identical twins' TCR repertoires revealed by high-throughput sequencing

(2014) I. V. Zvyagin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

High-resolution antibody dynamics of vaccine-induced immune responses

(2014) Uri Laserson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Diversity and clonal selection in the human T-cell repertoire

(2014) Q. Qi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Studying the antibody repertoire after vaccination: practical applications

(2014) Jacob D. Galson et al.   TRENDS IN IMMUNOLOGY

Convergent Antibody Signatures in Human Dengue

(2013) Poornima Parameswaran et al.   Cell Host & Microbe

Deep mutational scanning of an antibody against epidermal growth factor receptor using mammalian cell display and massively parallel pyrosequencing

(2013) Charles M. Forsyth et al.   mAbs

Genetic measurement of memory B-cell recall using antibody repertoire sequencing

(2013) C. Vollmers et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Lineage Structure of the Human Antibody Repertoire in Response to Influenza Vaccination

(2013) N. Jiang et al.   Science Translational Medicine

Multi Step Selection in Ig H Chains is Initially Focused on CDR3 and Then on Other CDR Regions

(2013) Gilad Liberman et al.   Frontiers in Immunology

Robust in vitro affinity maturation strategy based on interface-focused high-throughput mutational scanning

(2012) Yasuhiro Fujino et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Statistical inference of the generation probability of T-cell receptors from sequence repertoires

(2012) A. Murugan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Mechanism for TCR Sharing between T Cell Subsets and Individuals Revealed by Pyrosequencing

(2011) Vanessa Venturi et al.   JOURNAL OF IMMUNOLOGY

Phage Display for the Generation of Antibodies for Proteome Research, Diagnostics and Therapy

(2011) Thomas Schirrmann et al.   MOLECULES

High-resolution mapping of protein sequence-function relationships

(2010) Douglas M Fowler et al.   NATURE METHODS

Using deep sequencing to characterize the biophysical mechanism of a transcriptional regulatory sequence

(2010) J. B. Kinney et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Maximum entropy models for antibody diversity

(2010) T. Mora et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Overlap and Effective Size of the Human CD8+ T Cell Receptor Repertoire

(2010) H. S. Robins et al.   Science Translational Medicine

Comprehensive assessment of T-cell receptor  -chain diversity in    T cells

(2009) H. S. Robins et al.   BLOOD

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

High-Throughput Sequencing of the Zebrafish Antibody Repertoire

(2009) J. A. Weinstein et al.   SCIENCE

Measurement and Clinical Monitoring of Human Lymphocyte Clonality by Massively Parallel V-D-J Pyrosequencing

(2009) S. D. Boyd et al.   Science Translational Medicine