Review
Biotechnology & Applied Microbiology
Vyoma Mistry, Siddhi Darji, Pragya Tiwari, Abhishek Sharma
Summary: Catharanthus roseus is a medicinal plant known for biosynthesizing anticancer monoterpenoid indole alkaloids, with more than 130 bioactive compounds synthesized. Due to their cytotoxic nature, the production of these compounds is low in plants. Research has attempted to increase production through plant tissue culture and metabolic engineering.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Changying Huang, Liyang Peng, Xinqi Xu, Yunshu Lu, Xueting Wang, Zhongming Lan, Jingdi Chen, Yanxia Zhou
Summary: Chitosan nanoparticles carrying chloramphenicol (CAP-CNPs) were prepared using ionotropic gelation technique and incorporated into a thermosensitive in situ gel (CAP-CNPs-Gel). The optimized CAP-CNPs-Gel demonstrated sustained drug release and superior antibacterial activity, making it a reliable treatment for eye infections.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Khansa Jamil, Sahir Hameed Khattak, Anum Farrukh, Sania Begum, Muhammad Naeem Riaz, Aish Muhammad, Tahira Kamal, Touqeer Taj, Imran Khan, Sundus Riaz, Huma Batool, Kaleemullah Mandokhail, Sabahat Majeed, Sajid Ali Khan Bangash, Alia Mushtaq, Shahab Bashir, Imdad Kaleem, Fahed Pervaiz, Aamir Rasool, Muhammad Ammar Amanat, Ghulam Muhammad Ali
Summary: This study investigated the cytotoxic and antidiabetic effects of greenly synthesized silver nanoparticles (C. AgNPs) from Catharanthus roseus methanolic extract. The results showed that C. AgNPs exhibited positive potential on Vero cell lines and experimental rats. The study also revealed a significant difference in various parameters between the control and diabetic groups of rats. The findings highlight the importance of C. AgNPs in nanotherapeutics.
Article
Plant Sciences
F. A. S. Hassan, E. Ali, A. Gaber, M. Fetouh, R. Mazrou
Summary: Chitosan nanoparticles show significant potential in mitigating salt stress in Catharanthus roseus by activating antioxidant defense system and inducing gene expression, leading to higher alkaloid accumulation and better protection against salinity stress.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Dylan Levac, Paulo Cazares Flores, Vincenzo De Luca
Summary: This study describes the identification and functional expression of a N-methyltransferase in Catharanthus roseus, providing detailed information about the pathway for the formation of anticancer alkaloids.
Article
Materials Science, Multidisciplinary
Zhenyang Ji, Jinrong Yao, Zhengzhong Shao, Xin Chen
Summary: This study developed zein/chitosan composite nanospheres as carriers for controlled release of cinnamaldehyde (CA) in cosmetics industry. The structure of the nanospheres was influenced by the zein/chitosan mass ratio. The interaction between CA and zein, as well as the formation of Schiff base between CA and chitosan, effectively reduced the volatilization rate of CA and prolonged its release time.
Article
Polymer Science
Hudie Fu, Li Kang, Lanjun Yu, Yanping Fu, Zhijie Wen, Shengpeng Zhu, Mohan Zhao, Yuqing Wang, Qi Chen, Qinxiong Lin, Xinzhou Yang, Yan Hu
Summary: In this study, a gel film was prepared using chitosan and citric acid, and ketoconazole/beta-cyclodextrin inclusion complex was added to enhance its antibacterial effect. The composite film showed sustained drug release behavior, antibacterial effects, and biocompatibility, making it suitable for the treatment of cutaneous candidiasis.
JOURNAL OF APPLIED POLYMER SCIENCE
(2023)
Article
Polymer Science
Priya Dhiman, Meenakshi Bhatia
Summary: In this study, ketoconazole, an antifungal drug, was encapsulated in quaternized chitosan nanoparticles. The quaternization of chitosan was confirmed by proton nuclear magnetic resonance spectroscopy. The KCZ-QCSNPs were characterized using various techniques, and their antimicrobial activities were evaluated.
Article
Food Science & Technology
Masomeh Valizadeh, Mahdi Behnamian, Sara Dezhsetan, Roghayeh Karimirad
Summary: The study demonstrated that the use of turmeric oil incorporated chitosan nanoparticles (TEO-CSNPs) significantly enhanced the shelf life of white button mushrooms, extending the storage period of the fruit bodies. The treatment with TEO-CSNPs resulted in firmer mushrooms with lower color changes and microbial counts, as well as increased antioxidant enzyme activity and antioxidant content in the fruit bodies.
Article
Polymer Science
Carolina R. Inacio, Gabriel S. Nascimento, Ana Paula M. Barboza, Bernardo R. A. Neves, Angela Leao Andrade, Gabriel M. Teixeira, Lucas R. D. Sousa, Paula M. de A. Vieira, Katia M. Novack, Viviane M. R. dos Santos
Summary: In recent years, polymeric materials have gained attention in controlled release systems for drug administration. The use of polymeric matrices derived from polyethylene glycol (PEG) can provide controlled release of drugs and minimize adverse effects. The incorporation of ketoconazole with PEG 4000 and its derivatives showed changes in the film morphology observed by AFM. The release of ketoconazole followed first order kinetics for PEG 4000 HYDR INCORP and Higuchi kinetics for other samples.
Article
Engineering, Chemical
Acharya Balkrishna, Naveen Thakur, Bhavana Patial, Saurabh Sharma, Ashwani Kumar, Vedpriya Arya, Ryszard Amarowicz
Summary: Nanotechnology is a multidisciplinary field with the potential to solve pharmaceutical challenges. In this study, silver nanoparticles (Ag NPs) were successfully synthesized using plant extracts. The size and composition of the Ag NPs were analyzed using various techniques, and their inhibitory effects on Escherichia coli and Bacillus subtilis were studied. The results showed that the Ag NPs synthesized with Ocimum tenuiflorum extract had a stronger inhibitory effect on E. coli, and the antibacterial mechanism was attributed to the phytoconstituents and NPs.
Article
Multidisciplinary Sciences
Qi Chen, Yan Jin, Xiaorui Guo, Mingyuan Xu, Guanyun Wei, Xueyan Lu, Zhonghua Tang
Summary: Mechanical wounding is a novel approach to increase the biosynthetic production of specific secondary metabolites in plants. By stimulating mechanical damage to the upper and lower leaves, the production of secondary metabolites can be enhanced, offering new possibilities for the production practices of commercial plants.
Article
Plant Sciences
Ahmed Fouad, Adel E. Hegazy, Ehab Azab, Ebtihal Khojah, Tarek Kapiel
Summary: This study evaluates the capability of silver nanoparticles (AgNPs) in boosting alkaloids accumulation in Catharanthus roseus suspension cultures by examining the expression of C. roseus Mitogen Activated Protein Kinase 3 (CrMPK3) and Strictosidine Synthase (STR) genes. Results showed positive correlations between AgNPs concentration, oxidative stress indicators, enzyme activities, gene expression, and alkaloid accumulation. The findings suggest that AgNPs can induce alkaloid accumulation in plant cells through a signaling pathway involving hydrogen peroxide and MAPKs, leading to up-regulation of biosynthetic genes such as STR.
Article
Plant Sciences
E. F. Ali, A. M. El-Shehawi, O. H. M. Ibrahim, E. Y. Abdul-Hafeez, M. M. Moussa, F. A. S. Hassan
Summary: This study is the first to demonstrate the use of chitosan nanoparticles (CSNPs) in alleviating drought stress of Catharanthus roseus by enhancing antioxidant potential and gene expression related to alkaloid biosynthesis. CSNPs increased proline accumulation and the activity of catalase (CAT) and ascorbate peroxidase (APX), reducing drought-induced oxidative stress and preserving membrane integrity. Additionally, CSNPs led to increased alkaloid accumulation and upregulated gene expression associated with alkaloid biosynthesis under drought stress.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Fanfan Li, Stephen Bordeleau, Kyung Hee Kim, Jonathan Turcotte, Benjamin Davis, Lan Liu, Stephane Bayen, Vincenzo De Luca, Mehran Dastmalchi
Summary: This study described a Catharanthus roseus mutant that accumulates high levels of monoterpenoid indole alkaloids (MIAs). The mutant exhibited stunted growth and deficiency in chlorophyll biosynthesis, along with lesion formation. The lesions were enriched in MIAs and resulted from upregulation of genes involved in the MIA pathway. Furthermore, the accumulation of akuammicine, a minor constituent of the mutant, was found to be inducible by jasmonate and may have a role in plant defense.
Editorial Material
Pharmacology & Pharmacy
Fadia S. Youssef, Elisa Ovidi, Mahendra Rai
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Mahendra Rai, Beata Zimowska, Aniket Gade, Pramod Ingle
Summary: The increasing multidrug-resistance in pathogenic microbes and the emergence of new microbial pathogens have led to the search for new antimicrobials. This review focuses on the antimicrobial potential of secondary metabolites produced by different Phoma species and the biogenic synthesis of antimicrobial silver nanoparticles from Phoma.
Review
Microbiology
Mousa A. Alghuthaymi, Kamel A. Abd-Elsalam, Hussien M. AboDalam, Farah K. Ahmed, Mythili Ravichandran, Anu Kalia, Mahendra Rai
Summary: Traditional nanoparticle synthesis methods are expensive and hazardous, while microbial synthesis offers a green and rapid alternative for various applications. This review focuses on the synthesis of metal nanoparticles from Trichoderma species for agri-food applications and discusses their potential benefits and drawbacks.
Article
Biotechnology & Applied Microbiology
Magdalena Wypij, Maciej Ostrowski, Kamil Piska, Katarzyna Wojcik-Pszczola, Elzbieta Pekala, Mahendra Rai, Patrycja Golinska
Summary: Silver nanoparticles (AgNPs) synthesized using the actinobacterial SL19 strain showed remarkable antimicrobial activity against Escherichia coli and Pseudomonas aeruginosa, as well as cancer-specific sensitivity on human cancer cell lines. The AgNPs also had an impact on the catalytic and photocatalytic degradation of methyl orange. The study demonstrates the potential of AgNPs as antibacterial and anticancer agents, but notes that properties and activity may vary depending on the synthesized batch.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Yulia Plekhanova, Mahendra Rai, Anatoly N. Reshetilov
Summary: This review discusses the potential of nanomaterials in improving the basic parameters of bioelectrochemical systems, including sensitivity increase, detection lower-limit decrease, detection-range change, lifetime increase, and substrate-specificity control. The application of nanomaterials enhances the functioning efficiency of these devices and expands their spheres of use.
Article
Biotechnology & Applied Microbiology
Satyam Sangeet, Sarika Pawar, Neelu Nawani, Manisha Junnarkar, Swapnil Gaikwad
Summary: This study investigated the computational docking approach of biofabricated silver nanoparticles against P. aeruginosa virulent exoenzymes. The synthesis and characterization of biofabricated silver nanoparticles using Piper betle leaves were carried out. The computational docking analysis showed that the nanoparticles interacted with the active sites of the exoenzymes and inhibited their adverse effects on host cells. Therefore, biofabricated silver nanoparticles hold promise as anti-infective nanomedicine agents.
Article
Plant Sciences
Renata da Silva Carneiro, Marta Rejane Canuto, Lara Kelly Ribeiro, Danniel Cabral Leao Ferreira, Amanda Fonseca Costa Assuncao, Clara Andrezza Crisostomo Bezerra Costa, Johnnatan Duarte de Freitas, Mahendra Rai, Laecio Santos Cavalcante, Wellington dos Santos Alves, Antonio Luiz Martins Maia Filho, Jose Figueredo-Silva, Reginaldo da Silva Santos, Valdileia Teixeira Uchoa
Summary: This research evaluated the combination of Ximenia americana stem bark extract with ZnO/AgNPs for in vivo wound healing activity in mice. The results showed that the combination treatment reduced inflammation, increased collagen fiber deposition, and promoted skin lesion healing.
SOUTH AFRICAN JOURNAL OF BOTANY
(2022)
Review
Multidisciplinary Sciences
Pramod U. Ingle, Sudhir S. Shende, Prashant R. Shingote, Suchitra S. Mishra, Vaidehi Sarda, Dhiraj L. Wasule, Vishnu D. Rajput, Tatiana Minkina, Mahendra Rai, Svetlana Sushkova, Saglara Mandzhieva, Aniket Gade
Summary: Chitosan nanoparticles have great potential for various applications in agriculture due to their unique properties, such as biocompatibility, non-toxicity, biodegradability, and low allergenic. They can be used in wastewater treatment, plant growth promotion, fungicidal activity, wound healing, and tissue engineering. In addition, chitosan nanoparticles also exhibit anti-cancer, antifungal, and antioxidant activities, and can induce immune responses in plants, helping to manage biotic and abiotic stresses.
Review
Plant Sciences
Graciela Dolores Avila-Quezada, Mahendra Rai
Summary: This article discusses the emerging role of nano-materials in the detection and treatment of Phytophthora species, including slow delivery of agrochemicals. The authors propose integrating these tools into an integrated management program to reduce pesticide use and provide more effective and sustainable control of Phytophthora pathogens.
TRENDS IN PLANT SCIENCE
(2023)
Review
Biotechnology & Applied Microbiology
Mahendra Rai, Beata Zimowska, Aniket Gade, Pramod Ingle
Summary: The genus Phoma is a major group that secretes a wide range of secondary metabolites with various bioactivities. These metabolites, including phomenon, phomin, phomodione, cytochalasins, cercosporamide, phomazines, and phomapyrone, exhibit antimicrobial, antiviral, antinematode, and anticancer activities. This review highlights the importance of Phoma fungi as a natural source of biologically active secondary metabolites and their potential as anticancer agents.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Fernanda Wariss Figueiredo Bezerra, Jonilson de Melo e Silva, Gustavo Guadagnucci Fontanari, Johnatt Allan Rocha de Oliveira, Mahendra Rai, Renan Campos Chiste, Luiza Helena da Silva Martins
Summary: Nanopropolis, a natural nanomaterial with various applications, including food preservation and prevention of foodborne illness, has smaller size and higher antibacterial activity compared to common propolis.
Review
Chemistry, Physical
Szymon Graczyk, Robert Paslawski, Arkadiusz Grzeczka, Urszula Paslawska, Beata Swieczko-Zurek, Klaudia Malisz, Ketul Popat, Alina Sionkowska, Patrycja Golinska, Mahendra Rai
Summary: Microbial colonization in veterinary stents is a significant issue in veterinary medicine. Prolonged colonization can lead to infections, inflammation, and compromise the effectiveness of the stents, affecting the overall well-being of animal patients. Managing and mitigating the impact of these pathogens is a crucial challenge that veterinarians and researchers are actively addressing.
Article
Biochemistry & Molecular Biology
Abdul Arif Khan, Sudhir K. Jain, Mahendra Rai, Samiran Panda
Summary: The study screened and analyzed the molecular basis and host targets of COVID-19-associated opportunistic fungal infections, identifying important host targets such as CARD9 and CYP51A1 for managing SARS-CoV2-associated fungal infections.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Review
Chemistry, Multidisciplinary
Graciela Dolores Avila-Quezada, Avinash P. Ingle, Patrycja Golinska, Mahendra Rai
Summary: Nano-fertilizers are emerging as a promising research field in agriculture, offering a novel way to optimize nutrient supply and increase global food production. The use of nanocarriers can reduce the amount of active chemicals deposited in plants and provide slow-release nutrients. This review addresses the potential benefits, synthesis, mode of entry, mechanisms of action, and fate of nanomaterials in soil for NFs in agriculture.
NANOTECHNOLOGY REVIEWS
(2022)
Review
Infectious Diseases
Mahendra Rai, Shital Bonde, Alka Yadav, Yulia Plekhanova, Anatoly Reshetilov, Indarchand Gupta, Patrycja Golinska, Raksha Pandit, Avinash P. Ingle
Summary: The COVID-19 pandemic has put global pressure on the healthcare system, necessitating the development of rapid tests for early detection and bulk screening of infected individuals. Nanotechnology provides promising solutions for diagnosis, drug delivery, and therapy. This review discusses the use of nanomaterials for COVID-19 control, including diagnosis, vaccines, and drug delivery systems.
EXPERT REVIEW OF ANTI-INFECTIVE THERAPY
(2022)