Article
Cell & Tissue Engineering
Marc Vila Cuenca, Amy Cochrane, Francijna E. van den Hil, Antoine A. F. de Vries, Saskia A. J. Lesnik Oberstein, Christine L. Mummery, Valeria V. Orlova
Summary: The study developed a 3D vessel-on-chip model based on human induced pluripotent stem cells to recapitulate the crosstalk between endothelial cells and pericytes or vascular smooth muscle cells, enabling the study of vascular (patho)physiology and therapy. The hiPSC-derived vascular cells organized into stable microvascular networks within a fibrin hydrogel microenvironment and responded to vasoactive stimulation, demonstrating the model's potential for quantifying vascular organization and intracellular Ca2+ release kinetics.
Article
Obstetrics & Gynecology
Xinxin Zhu, Fei Yu, Guijun Yan, Yali Hu, Haixiang Sun, Lijun Ding
Summary: Human endometrial CD34(+) adventitial cells located in the outermost layer of blood vessels and CD146(+) pericytes surrounding inner endothelial cells of microvessels showed MSC phenotypes in in vitro culture, but had limited potential for endometrium regeneration.
HUMAN REPRODUCTION
(2021)
Article
Oncology
Zong-Qing Zheng, Jin-Tao Chen, Ming-Cheng Zheng, Li-Juan Yang, Jun-Ming Wang, Quan-Li Liu, Lu-Fei Chen, Zu-Cheng Ye, Jin-Ming Lin, Zhi-Xiong Lin
Summary: This study identified the critical role of Nestin(+)/CD31(+) cells in HPVNs, showing their involvement in GBM chemoresistance. Nestin(+)/CD31(+) cells were found to promote GSLC chemoresistance by providing JAG1 and DLL4, with Hes1 expression correlating with poor prognosis in GBM HPVN tumor cells rather than total Hes1 expression in GBM tissues.
Article
Cell Biology
Lei Tang, Chaoyang Zhang, Qian Yang, Hai Xie, Dandan Liu, Haibin Tian, Lixia Lu, Jing-Ying Xu, Weiye Li, Guoxu Xu, Qinghua Qiu, Kun Liu, Dawei Luo, Guo-Tong Xu, Jingfa Zhang
Summary: Melatonin has protective effects on the inner blood-retinal barrier in diabetic retinopathy by regulating the expression of inflammatory factors and tight junction proteins. This study provides insights into the development of melatonin-based therapy for diabetic retinopathy.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Cell Biology
Turkuler Ozgumus, Oksana Sulaieva, Ruchi Jain, Isabella Artner, Valeriya Lyssenko
Summary: Perinatal exposure to starvation may lead to the development of severe retinopathy in adult patients with diabetes, as short-term glucose starvation affects the gene expression of mouse embryonic retinal cells, especially genes regulating development, energy metabolism, and function maintenance of retinal neurons, glial cells, and vascular elements.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Chiara Simone Middel, Nadine Dietrich, Hans-Peter Hammes, Jens Kroll
Summary: The retinal trypsin digest protocol provides novel insights into the zebrafish retinal vascular architecture. Quantification of the different cell types shows that zebrafish have higher numbers of mural cells and an increased mural cell to endothelial cell ratio compared to mammalian retina.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Neurosciences
Aude Couturier, Guillaume Blot, Lucile Vignaud, Celine Nanteau, Amelie Slembrouck-Brec, Valerie Fradot, Niyazi Acar, Jose-Alain Sahel, Ramin Tadayoni, Gilles Thuret, Florian Sennlaub, Jerome E. Roger, Olivier Goureau, Xavier Guillonneau, Sacha Reichman
Summary: Muller glial cells play a crucial role in maintaining retinal homeostasis and metabolism, but reliable human cell sources for modeling MGC-associated diseases are limited. The newly developed hiMGCs exhibit phenotypic and transcriptomic similarities to pMGCs, making them valuable tools for disease modeling, such as diabetic retinopathy.
Review
Cell & Tissue Engineering
Judith Lechner, Reinhold J. Medina, Noemi Lois, Alan W. Stitt
Summary: Diabetic retinopathy, a complication of diabetes, is a leading cause of vision loss in working age adults. The loss of integrity of the retinal neurovascular unit is central to the disease pathogenesis. Current treatment options are limited to late-stages of the disease, and new treatments are needed to target early stages and prevent vision loss.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell & Tissue Engineering
Kee-Pyo Kim, Cui Li, Daria Bunina, Hyun-Woo Jeong, Julia Ghelman, Juyong Yoon, Borami Shin, Hongryeol Park, Dong Wook Han, Judith B. Zaugg, Johnny Kim, Tanja Kuhlmann, Ralf H. Adams, Kyung-Min Noh, Steven A. Goldman, Hans R. Schoeler
Summary: By transducing an optimized transcription factor combination into a permissive donor phenotype, pericyte-derived iOPCs (PC-iOPCs) can overcome the limitations of inefficient generation and limited expansion and differentiation competence. PC-iOPCs are stably expandable and functionally myelinogenic with high differentiation competence.
Article
Cell Biology
Natalia A. Gomes, Isabella B. Valle, Frederico O. Gleber-Netto, Tarcilia A. Silva, Hortencia M. de Castro Oliveira, Rafaela F. Oliveira, Luiza de Almeida Q. Ferreira, Lia S. Castilho, Paulo H. R. G. Reis, Pedro H. D. M. Prazeres, Gustavo B. Menezes, Claudia S. Magalhaes, Ricardo A. Mesquita, Marcia M. Marques, Alexander Birbrair, Ivana M. A. Diniz
Summary: This study used Nestin-GFP/NG2-DsRed mice to identify different subsets of perivascular cells in dental pulp tissue. Photobiomodulation (PBM) treatment significantly increased the number of pericytes, particularly in the more committed subset. PBM also stimulated blood vessel sprouting adjacent to the injury site and maintained signs of pulp vitality.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Cell Biology
Ze-Hui Shi, Xiao-Yan Han, Mu-Di Yao, Chang Liu, Qin Jiang, Biao Yan
Summary: This study identified 72 miRNAs that were differentially expressed in EPCs following high glucose stress, with target genes involved in protein binding, cell differentiation, and cytoskeleton based on GO analysis. KEGG pathway analysis revealed the association between cGMP-PKG signaling pathway and miRNA-mediated EPC function. The up-regulation of miR-375-3p in clinical samples of DR patients was verified, and its inhibition protected EPCs against hyperglycemic or hypoxic stress-induced injury, enhancing cell viability, proliferation, migration, tube formation ability, and delaying apoptosis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Francesca Lazzara, Anna Maria Longo, Giovanni Giurdanella, Gabriella Lupo, Chiara Bianca Maria Platania, Settimio Rossi, Filippo Drago, Carmelina Daniela Anfuso, Claudio Bucolo
Summary: Vitamin D-3 plays a positive role in protecting the blood retinal barrier and attenuating cell damage in diabetic retinopathy through regulating inflammatory pathways.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Cell Biology
Dong Lin, Ruijie Qin, Lixin Guo
Summary: DR is a common complication of diabetes, with high TSH levels being associated with an increased prevalence; TSH may regulate glucose-induced PCs loss through mitochondrial apoptosis involving TSHR.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Review
Cell Biology
Shuo Yang, Shounan Qi, Chenguang Wang
Summary: Diabetic retinopathy is a significant complication of diabetes, and the activation and changes in retinal Muller cells play a crucial role in its pathogenesis. Understanding the involvement of Muller cells can provide new targets for the treatment of diabetic retinopathy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Endocrinology & Metabolism
Tian Niu, Junwei Fang, Xin Shi, Mengya Zhao, Xindan Xing, Yihan Wang, Shaopin Zhu, Kun Liu
Summary: Diabetic retinopathy (DR) is a major cause of visual impairment in middle-aged individuals. This study used single-cell RNA sequencing (scRNA-seq) to analyze the retina in a model of type 2 diabetes, identifying cell types and specific gene expressions associated with DR. The study suggests retinaldehyde-binding protein 1 (RLBP1) as a potential therapeutic target for DR, providing new insights into pathogenic factors for treating DR and related dysfunctions.
Article
Hematology
Max van Lessen, Masanori Nakayama, Katsuhiro Kato, Jung Mo Kim, Kozo Kaibuchi, Ralf H. Adams
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2015)
Article
Cell Biology
Urs H. Langen, Mara E. Pitulescu, Jung Mo Kim, Rocio Enriquez-Gasca, Kishor K. Sivaraj, Anjali P. Kusumbe, Amit Singh, Jacopo Di Russo, M. Gabriele Bixel, Bin Zhou, Lydia Sorokin, Juan M. Vaquerizas, Ralf H. Adams
NATURE CELL BIOLOGY
(2017)
Article
Multidisciplinary Sciences
Hyun-Woo Jeong, Benjamin Hernandez-Rodriguez, JungMo Kim, Kee-Pyo Kim, Rocio Enriquez-Gasca, Juyong Yoon, Susanne Adams, Hans R. Schoeler, Juan M. Vaquerizas, Ralf H. Adams
NATURE COMMUNICATIONS
(2017)
Article
Hematology
Ji Yeon Kim, Jun-Sub Choi, Sun-Hwa Song, Ji-Eun Im, Jung-Mo Kim, Kyungjong Kim, Soonboem Kwon, Hwa Kyoung Shin, Choun-Ki Joo, Byung Ho Lee, Wonhee Suh
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2014)
Article
Engineering, Biomedical
Ji S. Park, Kun Na, Dae G. Woo, Han N. Yang, Jung M. Kim, Jae H. Kim, Hyung-Min Chung, Keun-Hong Park
Article
Engineering, Biomedical
Soon-Jung Park, Sung-Hwan Moon, Hye-Jin Lee, Joa-Jin Lim, Jung-Mo Kim, Joseph Seo, Ji-Woon Yoo, Ok-Jung Kim, Sun-Woong Kang, Hyung-Min Chung
Article
Cell & Tissue Engineering
Jung Mo Kim, Sung-Hwan Moon, Sung Geum Lee, Youn Jeong Cho, Ki Sung Hong, Jae Ho Lee, Hey Jin Lee, Hyung-Min Chung
STEM CELLS AND DEVELOPMENT
(2011)
Article
Cell & Tissue Engineering
Won Yun Choi, Hwang Gyun Jeon, Young Chung, Jung Jin Lim, Dong Hyuk Shin, Jung Mo Kim, Byeong Seong Ki, Seung-Hun Song, Seong-Jun Choi, Keun-Hong Park, Sung Han Shim, Jisook Moon, Sung Jun Jung, Hyun Mi Kang, Seah Park, Hyung Min Chung, Jung Jae Ko, Kwang Yul Cha, Tae Ki Yoon, Haekwon Kim, Dong Ryul Lee
STEM CELLS AND DEVELOPMENT
(2013)
Article
Cell & Tissue Engineering
Jung Mo Kim, Sung-Hwan Moon, Soon-Jung Park, Ha Young Lee, Ki-Sung Hong, Joseph Seo, Yoe-Sik Bae, Hyung-Min Chung
STEM CELLS AND DEVELOPMENT
(2013)
Review
Biochemical Research Methods
JungMo Kim, Maria Gabriele Bixel
Article
Multidisciplinary Sciences
Seung-hwan Jeong, Myung Jin Yang, Seunghyeok Choi, JungMo Kim, Gou Young Koh
Summary: STING agonists have been shown to induce early apoptosis of endothelial cells in subcutaneous xenografts models, while additional inhibition of AKT is required for efficient induction of apoptosis in primary tumors. This study proposes a more advanced avenue for treating primary tumors that are refractory to single STING agonist therapy.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Jung Hyun Bae, Myung Jin Yang, Seung-Hwan Jeong, JungMo Kim, Seon Pyo Hong, Jin Woo Kim, Yoo Hyung Kim, Gou Young Koh
Summary: In this study, the researchers discovered that Merlin, encoded by the neurofibromatosis type 2 (NF2) gene, suppresses VEGFR2 internalization and controls the induction of tip endothelial cells (EC). Depletion of Nf2 in endothelial cells enhances VEGFR2 internalization and leads to excessive filopodia formation in tip EC induction. Merlin binds to the VEGFR2-VE-cadherin complex and reduces downstream signaling, thus affecting the induction of tip ECs.
Article
Medicine, Research & Experimental
Ji Hoon Ahn, JungMo Kim, Seon Pyo Hong, Sung Yong Choi, Myung Jin Yang, Young Seok Ju, Young Tae Kim, Ho Min Kim, Md Tazikur Rahman, Man Ki Chung, Sang Duk Hong, Hosung Bae, Chang-Seop Lee, Gou Young Koh
Summary: Research shows that in the early stages of COVID-19, the virus replicates massively in multiciliated cells of the nasal epithelium, and infected ciliated cells are rapidly replaced by differentiating precursor cells.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Meeting Abstract
Immunology
Yoe-Sik Bae, Sang Doo Kim, Jung Mo Kim, Ha Young Lee, Jae Woong Shim, Hak Jung Kim, Su-Kil Seo, Jeanho Yun
JOURNAL OF IMMUNOLOGY
(2010)