On the Spatial Organization of mRNA, Plasmids, and Ribosomes in a Bacterial Host Overexpressing Membrane Proteins

Quantitative Localization Microscopy Reveals a Novel Organization of a High-Copy Number Plasmid

(2016) Yong Wang et al.   BIOPHYSICAL JOURNAL

Spatial organization of bacterial transcription and translation

(2016) Michele Castellana et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

ppGpp negatively impacts ribosome assembly affecting growth and antimicrobial tolerance in Gram-positive bacteria

(2016) Rebecca M. Corrigan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Spatial organization shapes the turnover of a bacterial transcriptome

(2016) Jeffrey R Moffitt et al.   eLife

iSBatch: a batch-processing platform for data analysis and exploration of live-cell single-molecule microscopy images and other hierarchical datasets

(2015) Victor E. A. Caldas et al.   Molecular BioSystems

Oufti: an integrated software package for high-accuracy, high-throughput quantitative microscopy analysis

(2015) Ahmad Paintdakhi et al.   MOLECULAR MICROBIOLOGY

Model Uracil-Rich RNAs and Membrane Protein mRNAs Interact Specifically with Cold Shock Proteins in Escherichia coli

(2015) Daniel Benhalevy et al.   PLoS One

Determination of in vivo target search kinetics of regulatory noncoding RNA

(2015) J. Fei et al.   SCIENCE

Optimizing membrane-protein biogenesis through nonoptimal-codon usage

(2014) Alexey S Morgunov et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Local slowdown of translation by nonoptimal codons promotes nascent-chain recognition by SRP in vivo

(2014) Sebastian Pechmann et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Single-particle tracking reveals that free ribosomal subunits are not excluded from the Escherichia coli nucleoid

(2014) A. Sanamrad et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Signal Recognition Particle: An Essential Protein-Targeting Machine

(2013) David Akopian et al.   Annual Review of Biochemistry

Benchmarking Various Green Fluorescent Protein Variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for Live Cell Imaging

(2013) Wout Overkamp et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Bacterial-based membrane protein production

(2013) Susan Schlegel et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

Improved membrane protein expression in Lactococcus lactis by fusion to Mistic

(2013) Y. Xu et al.   MICROBIOLOGY-SGM

Measuring mRNA copy number in individual Escherichia coli cells using single-molecule fluorescent in situ hybridization

(2013) Samuel O Skinner et al.   Nature Protocols

How to get (a)round: mechanisms controlling growth and division of coccoid bacteria

(2013) Mariana G. Pinho et al.   NATURE REVIEWS MICROBIOLOGY

High-copy bacterial plasmids diffuse in the nucleoid-free space, replicate stochastically and are randomly partitioned at cell division

(2013) R. Reyes-Lamothe et al.   NUCLEIC ACIDS RESEARCH

Role of mRNA Stability during Bacterial Adaptation

(2013) Clémentine Dressaire et al.   PLoS One

Superresolution imaging of ribosomes and RNA polymerase in live Escherichia coli cells

(2012) Somenath Bakshi et al.   MOLECULAR MICROBIOLOGY

Gene location and DNA density determine transcription factor distributions in Escherichia coli

(2012) Thomas E Kuhlman et al.   Molecular Systems Biology

Membrane protein expression triggers chromosomal locus repositioning in bacteria

(2012) E. A. Libby et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

pSEUDO, a Genetic Integration Standard for Lactococcus lactis

(2011) Joao P. C. Pinto et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Subdiffraction-Limit Study of Kaede Diffusion and Spatial Distribution in Live Escherichia coli

(2011) Somenath Bakshi et al.   BIOPHYSICAL JOURNAL

RNase Y in Bacillus subtilis: a Natively Disordered Protein That Is the Functional Equivalent of RNase E from Escherichia coli

(2011) M. Lehnik-Habrink et al.   JOURNAL OF BACTERIOLOGY

Consequences of the Overexpression of a Eukaryotic Membrane Protein, the Human KDEL Receptor, in Escherichia coli

(2011) Mirjam M. Klepsch et al.   JOURNAL OF MOLECULAR BIOLOGY

MicrobeTracker: quantitative image analysis designed for the smallest organisms

(2011) Ethan C. Garner   MOLECULAR MICROBIOLOGY

The Response of Lactococcus lactis to Membrane Protein Production

(2011) Ravi K. R. Marreddy et al.   PLoS One

Efficient Overproduction of Membrane Proteins in Lactococcus lactis Requires the Cell Envelope Stress Sensor/Regulator Couple CesSR

(2011) Joao P. C. Pinto et al.   PLoS One

Reprogramming chaperone pathways to improve membrane protein expression in Escherichia coli

(2011) Brent L. Nannenga et al.   PROTEIN SCIENCE

Translation-Independent Localization of mRNA in E. coli

(2011) K. Nevo-Dinur et al.   SCIENCE

A mutant Pfu DNA polymerase designed for advanced uracil-excision DNA engineering

(2010) Morten HH Nørholm   BMC BIOTECHNOLOGY

Quantitative and spatio-temporal features of protein aggregation in Escherichia coli and consequences on protein quality control and cellular ageing

(2010) Juliane Winkler et al.   EMBO JOURNAL

Combinatorial Method for Overexpression of Membrane Proteins inEscherichia coli

(2010) Shani Leviatan et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Evolved Lactococcus lactis Strains for Enhanced Expression of Recombinant Membrane Proteins

(2010) Daniel M. Linares et al.   JOURNAL OF MOLECULAR BIOLOGY

Spatial organization of the flow of genetic information in bacteria

(2010) Paula Montero Llopis et al.   NATURE

Amino Acid Accumulation Limits the Overexpression of Proteins in Lactococcus lactis

(2010) Ravi K. R. Marreddy et al.   PLoS One

Single-Molecule Approaches to Stochastic Gene Expression

(2009) Arjun Raj et al.   Annual Review of Biophysics

Stress-Responsive Systems Set Specific Limits to the Overproduction of Membrane Proteins in Bacillus subtilis

(2009) J. C. Zweers et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Studying membrane proteins through the eyes of the genetic code revealed a strong uracil bias in their coding mRNAs

(2009) J. Prilusky et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Rational design of a fusion partner for membrane protein expression inE. coli

(2009) Jianying Luo et al.   PROTEIN SCIENCE

Plasmid pCS1966, a New Selection/Counterselection Tool for Lactic Acid Bacterium Strain Construction Based on the oroP Gene, Encoding an Orotate Transporter from Lactococcus lactis

(2008) C. Solem et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Towards the development of Bacillus subtilis as a cell factory for membrane proteins and protein complexes

(2008) Jessica C Zweers et al.   Microbial Cell Factories

The global, ppGpp-mediated stringent response to amino acid starvation in Escherichia coli

(2008) Matthew F. Traxler et al.   MOLECULAR MICROBIOLOGY

Tuning Escherichia coli for membrane protein overexpression

(2008) S. Wagner et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Protein Translocation Across the Bacterial Cytoplasmic Membrane

(2007) Arnold J.M. Driessen et al.   Annual Review of Biochemistry