Review
Biochemistry & Molecular Biology
Efrosini Artikis, Allison Kraus, Byron Caughey
Summary: The structures of infectious prion fibrils have been revealed using cryo-EM, providing insights into the structural features that contribute to pathogenicity. Variations in the conformation and sequence of PrP fibrils likely contribute to strain-dependent effects, while posttranslational modifications may play a role in membrane binding and pathogenesis.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Allison Kraus, Forrest Hoyt, Cindi L. Schwartz, Bryan Hansen, Efrosini Artikis, Andrew G. Hughson, Gregory J. Raymond, Brent Race, Gerald S. Baron, Byron Caughey
Summary: The study reveals the core structure of prions and the templating surface formed by individual monomers at fibril ends. Comparison to another prion strain shows differences in fibril morphology. These findings provide insights into the propagation of prions.
Article
Microbiology
Alyssa J. Block, Ronald A. Shikiya, Thomas E. Eckland, Anthony E. Kincaid, Ryan W. Walters, Jiyan Ma, Jason C. Bartz
Summary: Murine synthetic prions can efficiently establish infection in hamsters and quickly adapt, showing similar clinical and biochemical properties to known prion strains. This model could be valuable for exploring the structure-function relationships between PrPSc and PrP(C) from different species.
Review
Biochemistry & Molecular Biology
Byron Chalidis, Panagiotis Givissis, Pericles Papadopoulos, Charalampos Pitsilos
Summary: Platelet-rich plasma (PRP) has been widely used in ligament and tendon injuries, with varying effects on cellular activities and tissue regeneration. In vitro studies have shown that PRP promotes cell proliferation, migration, viability, and collagen production. In vivo studies have demonstrated increased fibroblastic activity, collagen production, and vascularity in ligament and tendon tissues after PRP application. However, there is controversy regarding the optimal timing and dosage of PRP, as well as its potential negative effects on regeneration. Standardized protocols are needed to address these issues.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Multidisciplinary Sciences
Hideyuki Takahashi, Stephen M. Strittmatter
Summary: Protein fibrils are known to accumulate in the brain during neurodegeneration, and now cryo-electron microscopy has revealed the high-resolution structures of TMEM106B fibrils, which were not previously thought to accumulate.
Review
Neurosciences
Asen Daskalov, Nadia El Mammeri, Alons Lends, Jayakrishna Shenoy, Gaelle Lamon, Yann Fichou, Ahmad Saad, Denis Martinez, Estelle Morvan, Melanie Berbon, Axelle Grelard, Brice Kauffmann, Mathias Ferber, Benjamin Bardiaux, Birgit Habenstein, Sven J. Saupe, Antoine Loquet
Summary: This article explores the structures of pathological and functional amyloids, discussing the obtained structural models through techniques such as solid-state NMR. The review concludes with a perspective on integrative approaches to structurally explore various aspects of prion biology.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2021)
Article
Biochemical Research Methods
Liangyong Yu, Ran Li, Xiangrui Zeng, Hongyi Wang, Jie Jin, Yang Ge, Rui Jiang, Min Xu
Summary: Utilizing deep learning for cross-domain subtomogram classification faces challenges, but by leveraging the distribution of unlabeled target domain data and the correlation between the source domain dataset and a few labeled target domain data, significant improvements can be achieved.
Review
Rehabilitation
Yundong Peng, Liang Du, Bowen Yang, Donghao Fan, Shaohui Jia, Cheng Zheng
Summary: This study investigated the effects of platelet-rich therapies on rotator cuff repair. The results showed that platelet-rich plasma (PRP) reduced the risk of repeat tears and improved clinical function scores, while platelet-rich fibrin (PRF) had no significant benefit. However, the number and heterogeneity of studies and methodological limitations limit the confidence in the true effect.
Article
Biochemistry & Molecular Biology
Kaitlyn E. Whitney, Grant J. Dornan, Jillian King, Jorge Chahla, Thos A. Evans, Marc J. Philippon, Robert F. LaPrade, Johnny Huard
Summary: This study compared fresh and freeze-thawed PRP preparations and found significant changes in matrix metalloproteinase (MMP) concentrations. MMPs play a crucial role in the remodeling phase of musculoskeletal injury.
Article
Biochemistry & Molecular Biology
Luis Concha-Marambio, Fei Wang, Enrique Armijo, Damian Gorski, Frank Ramirez, Andrew Scowcroft, Sandra Pritzkow, Claudio Soto
Summary: In this article, the authors describe a method for rapid and efficient generation of large quantities of highly infectious prions using the Protein Misfolding Cyclic Amplification (PMCA) technology. They also developed a bioreactor format that can continuously produce PrPSc without the need to add brain-derived prions. The LS-PMCA technology has been shown to work with various species and strains of prions, making it a valuable tool for biological and structural studies.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Editorial Material
Multidisciplinary Sciences
Michael Eisenstein
Summary: Scientists need to demonstrate the preservation of intact samples upon impact in order to link mass spectroscopy with cryo-electron microscopy and transform the understanding of complex protein structures.
Article
Multidisciplinary Sciences
Ankit Gupta, Mustafa Khammash
Summary: Adaptation is essential for organisms to survive and thrive in unpredictable environments. Maximal robust perfect adaptation (maxRPA) is a property that allows a variable to remain at a steady state value despite perturbations in network parameters. This study reveals that maxRPA imposes structural constraints on the network and provides a linear algebraic characterization for these constraints.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Yaxian Zhao, Bridget M. McVeigh, Vera Y. Moiseenkova-Bell
Summary: TRP ion channels are a super-family that mediate transmembrane cation flux in response to various stimuli. Recognized as potential pharmacological targets due to their role in human diseases, advances in cryo-EM TRP channel structural biology have shown diverse architectural elements and regulatory sites. This review focuses on the ligand-binding sites and regulatory mechanisms of TRP channels.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Virology
Chih-Yen King
Summary: This passage discusses the replication mechanism of prions and the emergence of variants and strains. It is suggested that the heat shock protein Hsp104 can restrict strain variation, leading to the possibility that some transmutable variants may have been mistaken as faithful-propagating. By altering the strength of Hsp104 in yeast, it is found that most variants are faithful-propagating, with only a few capable of changing to limited structural types.
Review
Biochemical Research Methods
Carlos H. M. Rodrigues, Douglas E. Pires, Tom L. Blundell, David B. Ascher
Summary: Protein-protein interfaces have unique binding geometry and physicochemical properties, with concave binding sites that challenge the previous belief about their flatness. A comprehensive review of protein-protein interface landscape reveals the utilization of small binding pockets even in larger flat interfaces.
BRIEFINGS IN BIOINFORMATICS
(2022)
Article
Biochemistry & Molecular Biology
Dana J. Levine, Jan Stoehr, Lillian E. Falese, Julian Ollesch, Holger Wille, Stanley B. Prusiner, Jeffrey R. Long
ACS CHEMICAL BIOLOGY
(2015)
Article
Biophysics
Lester Carter, Seung Joong Kim, Dina Schneidman-Duhovny, Jan Stoehr, Guillaume Poncet-Montange, Thomas M. Weiss, Hiro Tsuruta, Stanley B. Prusiner, Andrej Sali
BIOPHYSICAL JOURNAL
(2015)
Article
Biophysics
William Wan, Holger Wille, Jan Stoehr, Amy Kendall, Wen Bian, Michele McDonald, Sarah Tiggelaar, Joel C. Watts, Stanley B. Prusiner, Gerald Stubbs
BIOPHYSICAL JOURNAL
(2015)
Article
Biochemistry & Molecular Biology
William Wan, Jan Stoehr, Amy Kendall, Gerald Stubbs
Article
Multidisciplinary Sciences
Amanda L. Woerman, Jan Stoehr, Atsushi Aoyagi, Ryan Rampersaud, Zuzana Krejciova, Joel C. Watts, Takao Ohyama, Smita Patel, Kartika Widjaja, Abby Oehler, David W. Sanders, Marc I. Diamond, William W. Seeley, Lefkos T. Middleton, Steve M. Gentleman, Daniel A. Mordes, Thomas C. Suedhof, Kurt Giles, Stanley B. Prusiner
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2015)
Article
Chemistry, Multidisciplinary
Matthew R. Elkins, Tuo Wang, Mimi Nick, Hyunil Jo, Thomas Lemmin, Stanley B. Prusiner, William F. DeGrado, Jan Stohr, Mei Hong
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2016)
Article
Chemistry, Multidisciplinary
Jan Stoehr, Haifan Wu, Mimi Nick, Yibing Wu, Manasi Bhate, Carlo Condello, Noah Johnson, Jeffrey Rodgers, Thomas Lemmin, Srabasti Acharya, Julia Becker, Kathleen Robinson, Mark J. S. Kelly, Feng Gai, Gerald Stubbs, Stanley B. Prusiner, William F. DeGrado
Article
Multidisciplinary Sciences
Myungwoon Lee, Tuo Wang, Olga V. Makhlynets, Yibing Wu, Nicholas F. Polizzi, Haifan Wu, Pallavi M. Gosavi, Jan Stoehr, Ivan V. Korendovych, William F. DeGrado, Mei Hong
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Biochemistry & Molecular Biology
Mimi Nick, Yibing Wu, Nathan W. Schmidt, Stanley B. Prusiner, Jan Stohr, William F. DeGrado
Article
Multidisciplinary Sciences
Carlo Condello, Thomas Lemmin, Jan Stohr, Mimi Nick, Yibing Wu, Alison M. Maxwell, Joel C. Watts, Christoffer D. Caro, Abby Oehler, C. Dirk Keene, Thomas D. Bird, Sjoerd G. van Duinen, Lars Lannfelt, Martin Ingelsson, Caroline Graff, Kurt Giles, William F. DeGrado, Stanley B. Prusiner
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Cell Biology
Atsushi Aoyagi, Carlo Condello, Jan Stohr, Weizhou Yue, Brianna M. Rivera, Joanne C. Lee, Amanda L. Woerman, Glenda Halliday, Sjoerd van Duinen, Martin Ingelsson, Lars Lannfelt, Caroline Graff, Thomas D. Bird, C. Dirk Keene, William W. Seeley, William F. DeGrado, Stanley B. Prusiner
SCIENCE TRANSLATIONAL MEDICINE
(2019)
Meeting Abstract
Biochemistry & Molecular Biology
Carlo Condello, Atsushi Aoyagi, Jan Stohr, Weizhou Yue, Brianna M. Rivera, Joanne C. Lee, Amanda L. Woerman, Glenda Halliday, Sjoerd van Duinen, Martin Ingelsson, Lars Lannfelt, Caroline Graff, Thomas D. Bird, C. Dirk Keene, William W. Seeley, William F. DeGrado, Stanley B. Prusiner
Meeting Abstract
Biochemistry & Molecular Biology
Jan Stoehr, Carlo Condello, Abby Oehler, Stephen DeArmond, Holger Wille, Gerald Stubbs, Kurt Giles, Stanley Prusiner
Meeting Abstract
Biochemistry & Molecular Biology
Jan Stoehr, Carlo Condello, Joel C. Watts, Lillian Bloch, Abby Oehler, Mimi Nick, Manasi Bhate, Stephen J. DeArmond, Kurt Giles, William F. DeGrado, Stanley B. Prusiner