Article
Agriculture, Dairy & Animal Science
Anna Koseniuk, Grzegorz Smolucha, Malgorzata Natonek-Wisniewska, Anna Radko, Dominika Rubis
Summary: In this study, the researchers aimed to differentiate wild boars from domestic pigs by analyzing the polymorphisms within the MC1R and NR6A1 genes. They used PCR-RFLP and Real-time PCR techniques to study samples from wild boars in Poland and five pig breeds. The results showed that the NR6A1 g.748C > T variation is an effective marker for distinguishing between wild boars and domestic pigs. The study also revealed admixture between the two genomes using population genetics software.
Article
Multidisciplinary Sciences
John King N. Layos, Ronel B. Geromo, Dinah M. Espina, Masahide Nishibori
Summary: The genetic diversity and ancestry of domestic pigs in the Philippines were investigated using mitochondrial DNA analysis, revealing a close genetic connection to continental wild boars and supporting the hypothesis of multiple waves of human migrations to the Philippines. The results contribute to the conservation strategies and improvement of genetic resources in the Philippines.
Article
Virology
Dragan Brnic, Daniel Colic, Valentina Kunic, Nadica Maltar-Strmecki, Nina Kresic, Dean Konjevic, Miljenko Bujanic, Ivica Bacani, Drazen Hizman, Lorena Jemersic
Summary: This study investigated the prevalence and genetic diversity of RVA in domestic pigs and wild boars, and found a higher prevalence in domestic pigs compared to wild boars. Remarkable genetic diversity of RVA was observed in domestic pigs, with 23 different combinations identified. Interspecies transmission events were frequent between domestic pigs and wild boars, and even involved bovines and humans.
Article
Anthropology
Anibal G. Arregui
Summary: By examining the relationships between individuals, this study explores how human and wild boar interactions reshape the ecological landscape.
AMERICAN ETHNOLOGIST
(2023)
Article
Genetics & Heredity
Shuhao Fan, Chengcheng Kong, Yige Chen, Xianrui Zheng, Ren Zhou, Xiaodong Zhang, Xudong Wu, Wei Zhang, Yueyun Ding, Zongjun Yin
Summary: Using next-generation sequencing, we identified different copy number variations (CNVs) in 24 Anqingliubai pigs and 6 Asian wild boars to explore the relationship between genetic evolution and production traits. A total of 97,489 CNVs were identified, with 10,429 copy number variation regions (CNVRs) covering 32.06% of the porcine genome. Among the selected 96 CNVRs, 65 genes were found to be strongly correlated with traits such as growth, reproduction, detoxification, and fatty acid metabolism. The QTL overlapping regions were associated with meat traits, growth, and immunity.
Article
Microbiology
Manon Dechene-Tempier, Eric Jouy, Marie-Helene Bayon-Auboyer, Stephanie Bougeard, Claire Chauvin, Virginie Libante, Sophie Payot, Corinne Marois-Crehan
Summary: A study in France analyzed the antimicrobial resistance of 200 strains of Streptococcus suis, a zoonotic pathogen causing infections in pigs. Most strains showed resistance to at least one antibiotic, with higher resistance to macrolides-lincosamides and tetracycline. Resistance patterns varied based on geographical origin, serotypes, and isolate sources. The study highlights the importance of monitoring antimicrobial resistance in this zoonotic pathogen.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Limin Wei, Weiying Zhou, Zhaojing Zhu
Summary: This study compares the changes in the gut microbiota of wild boars and domestic pigs and finds significant differences between the two groups, indicating that the gut microbiota has changed during the evolution process. This provides some data references for the evolution of gut microbiota.
Article
Veterinary Sciences
Francisco Cortez Nunes, Teresa Letra Mateus, Emily Taillieu, Silvia Teixeira, Nuno Carolino, Alexandra Rema, Sofie De Bruyckere, Fatima Gartner, Freddy Haesebrouck, Irina Amorim
Summary: This study reveals the presence of Helicobacter species and F. gastrosuis in the stomach of pigs and wild boars, indicating a possible association with gastric disorders.
VETERINARY RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Samantha L. Bock, Christopher R. Smaga, Jessica A. McCoy, Benjamin B. Parrott
Summary: This study demonstrates the use of genome-wide DNA methylation (DNAm) patterns to investigate temperature-dependent sex determination (TSD) species, specifically American alligators, in the face of climate change. Through analyzing DNAm in blood cells of hatchling alligators, sex-associated differentially methylated cytosines (DMCs) and temperature-associated DMCs were identified. DNAm-based models were developed to accurately predict hatchling sex and past incubation temperature.
Article
Microbiology
Monika Moravkova, Iveta Kostovova, Katerina Kavanova, Radko Pechar, Stanislav Stanek, Ales Brychta, Michal Zeman, Tereza Kubasova
Summary: Restrictions on antibiotic use in pigs have led to the search for alternative probiotics. The absence of horizontally transmissible resistance genes is a key criterion for selecting probiotic bacteria. This study determined the antibiotic susceptibility profiles of 28 Lactobacillus amylovorus isolates from wild boars and farm pigs using the microdilution method and whole genome sequencing. The majority of L. amylovorus strains from domestic pigs showed resistance to tetracycline, erythromycin, and ampicillin. Analysis of horizontally transmissible genes revealed three genetic determinants, tetW, ermB, and aadE, with tetW and ermB genes only present in strains from domestic pigs.
Article
Evolutionary Biology
Zhimin Zhou, Tao Jiang, Yaling Zhu, Ziqi Ling, Bin Yang, Lusheng Huang
Summary: This study compares the genome-wide H3K27ac enhancer activities and mRNA profiling in domestic pigs and wild boars, revealing differential activity and overlapping genes related to mating behavior, growth factors and hormone response, and lipid metabolism. Nonredundant super-enhancers were also identified, along with differentially active peaks located close to or within candidate genes identified in DNA sequence-based population differentiation studies.
EVOLUTIONARY APPLICATIONS
(2022)
Article
Agriculture, Dairy & Animal Science
Xudong Wu, Ren Zhou, Yuanlang Wang, Wei Zhang, Xianrui Zheng, Guiying Zhao, Xiaodong Zhang, Zongjun Yin, Yueyun Ding
Summary: This study analyzed the genome of the local Chinese pig breed AQ pigs using resequencing technology and found that inbreeding levels were a concern and influenced by artificial selection. The study also identified genes related to immune biological processes in the ROH islands of AQ pigs.
Article
Veterinary Sciences
Seok-Chan Park, Suwon Kim, Tae-Won Jeong, Byungkwan Oh, Chae Woong Lim, Bumseok Kim
Summary: This study investigated the prevalence of porcine circovirus 2 (PCV2) and porcine circovirus 3 (PCV3) in slaughtered pigs and wild boars in Korea. The positive rates of PCV2 were higher in slaughtered pigs compared to wild boars, and the positive rates of PCV3 were also higher in slaughtered pigs compared to wild boars. Continuous surveillance and further study are needed for these emerging diseases.
VETERINARY MEDICINE AND SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Arnav Mehrotra, Bharat Bhushan, A. Karthikeyan, Akansha Singh, Snehasmita Panda, Meenu Bhati, Manjit Panigrahi, Triveni Dutt, P. Bishnu Mishra, Hubert Pausch, Amit Kumar
Summary: The study revealed that Ghurrah pigs in India have genetic ancestry from both Asian and European pigs, with signs of inter-species introgression. Selection analysis showed divergence in genomic regions associated with odontogenesis and nervous system processes from Asian and European breeds.
Article
Ecology
Anna Schleimer, Lorraine Richart, Frank Drygala, Francois Casabianca, Oscar Maestrini, Hannah Weigand, Chantal Schwartz, Michel Mittelbronn, Alain C. Frantz
Summary: Due to intensified domestification and hybridisation, the genetic distinctness of the Corsican wild boar has been found to be significantly different from domestic pigs. Molecular markers play a crucial role in assessing the impact of management practices on gene flow.
Article
Agriculture, Dairy & Animal Science
V. A. Balteanu, T. F. Cardoso, M. Amills, M. G. Luigi-Sierra, I. Egerszegi, I. Anton, A. Zsolnai
Summary: The Mangalitza lard-type pig breed, known for its fat appearance and curly hair, was created with four main lines through artificial selection in the nineteenth century. The study identified a region on chromosome 16 potentially influencing hair pigmentation in Mangalitza pigs, with the SLC45A2 gene as a candidate gene. The results suggest a strong association between specific genetic polymorphisms in SLC45A2 and the red and blond coat colors of Mangalitza pigs, indicating the impact of divergent directional selection on coat color in domestic species.
Correction
Multidisciplinary Sciences
Changfa Wang, Haijing Li, Yu Guo, Jinming Huang, Yan Sun, Jiumeng Min, Jinpeng Wang, Xiaodong Fang, Zicheng Zhao, Shuai Wang, Yanlin Zhang, Qingfeng Liu, Qiang Jiang, Xiuge Wang, Yijun Guo, Chunhong Yang, Yinchao Wang, Fang Tian, Guilong Zhuang, Yanna Fan, Qican Gao, Yuhua Li, Zhihua Ju, Jianbin Li, Rongling Li, Minghai Hou, Guiwen Yang, Guiqin Liu, Wenqiang Liu, Jiao Guo, Shanshan Pan, Guangyi Fan, Wei Zhang, Ruitao Zhang, Jie Yu, Xinhao Zhang, Qi Yin, Chuanliang Ji, Yuanchun Jin, Guidong Yue, Mei Liu, Jiake Xu, Shimin Liu, Jordi Jordana, Antonia Noce, Marcel Amills, Dong Dong Wu, Shuaicheng Li, Xiangshan Zhou, Jifeng Zhong
Summary: The paper has been corrected.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Yosra Ressaissi, Marcel Amills, Antonia Noce, Mohamed Ben Hamouda
Summary: The Arbi goat breed in Tunisia is an important economic resource with high mitochondrial diversity, possibly linked to the maritime diffusion during the Neolithic period. Analysis showed that the Arbi goats do not cluster with other goat breeds genetically, indicating potential historical crossbreeding practices.
BIOCHEMICAL GENETICS
(2021)
Article
Multidisciplinary Sciences
L. Criado-Mesas, N. Abdelli, A. Noce, M. Farre, J. F. Perez, D. Sola-Oriol, R. Martin-Venegas, A. Forouzandeh, F. Gonzalez-Sole, J. M. Folch
Summary: A custom gene expression panel was developed to assess gene expression changes in poultry under challenging conditions. Results showed higher expression of genes related to barrier function and nutrient transport in chickens raised in healthy conditions, while birds challenged with C.perfringens had higher expression of genes involved in immune response. Eimeria challenge altered expression of genes related to barrier function and metabolism, increasing expression of genes related to immune response and oxidative stress. This panel provides insights into broiler response to pathogen challenge and differences in gene expression patterns under coccidial vaccine or NE.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Christina Walz, Julia Brenmoehl, Nares Trakooljul, Antonia Noce, Caroline Caffier, Daniela Ohde, Martina Langhammer, Klaus Wimmers, Siriluck Ponsuksili, Andreas Hoeflich
Summary: The study investigated the effects of training and genetic predisposition on mRNA expression in the pituitary gland of a marathon mouse model, revealing a strong impact of genetic selection for enhanced running performance on gene expression in the pituitary gland. Training induced differential expression of genes in both mouse lines, but DUhTP mice showed resistance to the positive effects of training on protein and energy metabolism in the pituitary gland.
Editorial Material
Agriculture, Dairy & Animal Science
Maria Gracia Luigi-Sierra, Joaquim Casellas, Amparo Martinez, Juan Vicente Delgado, Javier Fernandez Alvarez, Francesc Xavier Such, Jordi Jordana, Marcel Amills
Article
Agriculture, Dairy & Animal Science
D. Guan, A. Martinez, M. G. Luigi-Sierra, J. Delgado, V Landi, A. Castello, J. Fernandez Alvarez, X. Such, J. Jordana, M. Amills
Summary: Artificial selection plays a major role in altering the genetic composition of livestock populations, with identifying genes under selection providing insight into their effects on phenotypic variation. A study on Murciano-Granadina goats identified 77 candidate genomic regions/SNPs, with significant selective sweeps on multiple chromosomes. RNA-Seq data revealed differential expression of 6414 genes during lactation, with 183 genes overlapping with selective sweeps related to various metabolic and developmental functions. The MC1R gene polymorphism was found to be the main determinant of black and brown colorations in these goats.
Article
Cell Biology
Mohsen Mesbah, Mohsen Forouzanfar, Shahin Eghbalsaied
Summary: The study found that supplementation of 3 and 5 mu M concentrations of estradiol can improve sperm viability and membrane integrity in goat semen, with the 3 mu M concentration also increasing the highest rate of progressive motility. However, a higher concentration of estradiol (5 mu M) reduced progressive motility. Additionally, there is a considerable interaction between EDTA and estradiol for sperm motility, with EDTA reducing the supportive effects of estradiol on sperm cryopreservation parameters.
BIOPRESERVATION AND BIOBANKING
(2022)
Article
Anatomy & Morphology
Shahin Eghbalsaied, Wilfried A. Kues
Summary: In this study, a protocol for electrotransfection of sheep embryonic fibroblast cells was developed. Optimal pulsing conditions and the addition of 3% dimethyl sulfoxide (DMSO) resulted in high transfection efficiency (>98%) and cell survival rate (>97%). CRISPR/Cas9-mediated gene editing of BMP15 and GDF9 genes in sheep EF cells was successfully achieved using the electroporation method.
CELLS TISSUES ORGANS
(2023)
Article
Reproductive Biology
Mehras Mazandarani, Farid Barati, Khosro Farahmand, Farzaneh Hoseini, Shahin Eghbalsaied
Summary: The study evaluated the implementation of ultrasound for monitoring gestation in mini-lop rabbits, demonstrating the effectiveness of ultrasound biometrics in accurately tracking pregnancy and detecting abnormal fetuses.
Article
Agriculture, Dairy & Animal Science
Emilio Marmol-Sanchez, Susanna Cirera, Laura M. Zingaretti, Mette Juul Jacobsen, Yuliaxis Ramayo-Caldas, Claus B. Jorgensen, Merete Fredholm, Taina Figueiredo Cardoso, Raquel Quintanilla, Marcel Amills
Summary: This study investigated the role of miRNAs in mRNA post-transcriptional regulation in porcine tissues. The results suggest that miRNAs play a more prominent role in the regulation of gene expression in the skeletal muscle system. Several genes related to energy homeostasis were also identified.
Article
Agriculture, Dairy & Animal Science
Martina Macri, Maria Gracia Luigi-Sierra, Dailu Guan, Juan Vicente Delgado, Javier Fernandez Alvarez, Marcel Amills, Amparo Martinez Martinez
Summary: Hematological traits are important indicators of health status. In humans, the genomic architecture of blood traits has been extensively studied, but the association between marker genotypes and hematological traits in goats has not been investigated yet.
Article
Multidisciplinary Sciences
Shahin Eghbalsaied, Wilfried A. Kues
Summary: In this study, large plasmids containing the CRISPR/Cas9 system and a puromycin transgene were used to evaluate the efficiency of targeted integration. The inclusion of nocodazole and RNase HII during electroporation significantly improved the rate of high-fidelity repair events.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Haonan Zeng, Wenjing Zhang, Qing Lin, Yahui Gao, Jinyan Teng, Zhiting Xu, Xiaodian Cai, Zhanming Zhong, Jun Wu, Yuqiang Liu, Shuqi Diao, Chen Wei, Wentao Gong, Xiangchun Pan, Zedong Li, Xiaoyu Huang, Xifan Chen, Jinshi Du, Fuping PigGTEx Consortium, Fuping Zhao, Yunxiang Zhao, Maria Ballester, Daniel Crespo-Piazuelo, Marcel Amills, Alex Clop, Peter Karlskov-Mortensen, Merete Fredholm, Pinghua Li, Ruihua Huang, Guoqing Tang, Mingzhou Li, Xiaohong Liu, Yaosheng Chen, Qin Zhang, Jiaqi Li, Xiaolong Yuan, Xiangdong Ding, Lingzhao Fang, Zhe Zhang
Summary: To unlock the potential of pigs in agriculture and biomedical research, the PigBiobank was created to investigate the molecular and cellular mechanisms underlying complex traits in pigs and translate the findings to other species.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Agriculture, Dairy & Animal Science
Emilio Marmol-Sanchez, Maria Gracia Luigi-Sierra, Anna Castello, Dailu Guan, Raquel Quintanilla, Raul Tonda, Marcel Amills
Summary: Our study identified SNPs in miRNA genes and their binding sites in porcine 3' UTR, and showed a reduced variability of porcine miRNA genes, suggesting strong purifying selection, particularly in the seed region. We also found significant associations between specific miRNA SNPs and lipid-related traits and hepatic gene expression in pigs, highlighting the importance of investigating the functional effects of miRNA polymorphisms outside the seed region.
GENETICS SELECTION EVOLUTION
(2021)