Article
Biochemistry & Molecular Biology
Hanna Brauninger, Tilo Thottakara, Jacob Schon, Svenja Voss, Vishnu Dhople, Svenja Warnke, Katharina Scherschel, Benedikt Schrage, Paulus Kirchhof, Stefan Blankenberg, Uwe Volker, Dirk Westermann, Elke Hammer, Diana Lindner
Summary: Fibroblasts are crucial in cardiac adaptation and pathological remodeling, with their secretome and transcriptome playing essential roles in myocardial fibrosis in diseases like dilated cardiomyopathy. Cytokines, such as TNF-alpha and TGF-beta, regulate the production of ECM proteins by fibroblasts, highlighting their involvement in pro-fibrotic and inflammatory processes in response to external stimuli.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Priyavathi Sureshkumar, Robson Augusto Souza dos Santos, Natalia Alenina, Stefan Mergler, Michael Bader
Summary: The G protein-coupled receptor MAS is the receptor for the endogenous ligand Angiotensin (Ang)-(1-7). This paper demonstrates the importance of MAS signaling in developing novel therapeutics for cardiovascular diseases. Ang-(1-7) induces calcium influx in MAS-transfected HEK293 cells, which is dependent on plasma membrane Ca2+ channels, phospholipase C, and protein kinase C. The study also reveals that MAS utilizes non-selective transient receptor potential channels (TRPs) for calcium entry.
Article
Developmental Biology
Daniel J. Toddie-Moore, Martti P. Montanari, Ngan Vi Tran, Evgeniy M. Brik, Hanna Antson, Isaac Salazar-Ciudad, Osamu Shimmi
Summary: Developmental patterning is regulated by conserved signaling pathways, with cell shape changes playing a critical role in pattern refinement. Mechano-chemical feedback leads to competition for developmental signals, affecting the process of pattern refinement.
DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Ze Wu, Lanlan Fang, Sizhu Yang, Yibo Gao, Zhen Wang, Qingxue Meng, Xuan Dang, Ying-Pu Sun, Jung-Chien Cheng
Summary: This study discovers a new biological function and underlying molecular mechanisms of GDF-11 in the regulation of human extravillous trophoblast cell invasion.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Biochemistry & Molecular Biology
Shiwei Xie, Chenyang Zhao, Wei Chen, Gengwu Li, Zhiwei Xiong, Xiangjun Tang, Fan Zhang, Heng Xiao
Summary: In patients with intervertebral disc degeneration (IDD), decreased levels of BMP were found, leading to impaired BMP/Smad signaling and increased Puma-dependent apoptosis. Knockdown of BMP receptors in nucleus pulposus cells mimicked the molecular changes observed in patients with IDD, and treatment with rhBMP2/7 significantly reduced levels of Puma protein and prevented cell apoptosis. These findings suggest a new mechanism by which BMPs contribute to IDD pathogenesis and provide evidence that rhBMPs may decrease apoptosis and improve outcomes.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Medicine, Research & Experimental
Chang Xu, Yu-han Ding, Kun Wang, Mengdi Hao, Huimin Li, Lei Ding
Summary: The study demonstrates that Cldn7 deficiency in CRC promotes stemness properties through Sox9-mediated Wnt/beta -catenin signaling. This finding elucidates the inhibitory role of Cldn7 in CRC and reveals a potential molecular mechanism, contributing to further research on Cldn7 and cancer stem cells.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Milan Kumar Lal, Eshita Sharma, Rahul Kumar Tiwari, Rajni Devi, Udit Nandan Mishra, Richa Thakur, Rucku Gupta, Abhijit Dey, Priyanka Lal, Awadhesh Kumar, Muhammad Ahsan Altaf, Durgesh Nandini Sahu, Ravinder Kumar, Brajesh Singh, Sunil Kumar Sahu
Summary: The interaction between nutrients and linked genes can reveal an individual's genetic response to specific nutrients. Understanding the field of nutrigenomics and nutrigenetics can contribute to optimizing health and disease prevention and treatment. Nutrient-mediated pathways regulated by hormone signals play a crucial role in detecting cellular nutrient concentrations, making them important in nutrition research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Xiao Li, Xinyuan Sun, Zhongyi Yan, Zhenxiang Zhao, Zhaojun Pang, Han Yang, Xiaoxin Ji, Yi Lei, Zixiang Zhu, Xiangqian Guo, Xin Mu
Summary: A study has found that an antibiotic called gramicidin A can enhance MDA5-mediated interferon signaling by increasing the oligomerization of MDA5 and dsRNA.
Article
Cell Biology
Bruna Moretto Rodrigues, Lucas Solla Mathias, Igor de Carvalho Depra, Sarah Santiloni Cury, Miriane de Oliveira, Regiane Marques Castro Olimpio, Maria Teresa De Sibio, Bianca Mariani Goncalves, Celia Regina Nogueira
Summary: This study provides new directions for elucidating the mechanisms of T3 action on osteoblast metabolism, with potential future implications for the treatment of endocrine-related bone pathologies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Ziying Chen, Flora Gordillo-Martinez, Lei Jiang, Pengcheng He, Wanzi Hong, Xuebiao Wei, Katherine A. Staines, Vicky E. Macrae, Chunxiang Zhang, Danqing Yu, Xiaodong Fu, Dongxing Zhu
Summary: The study suggests that zinc may act as a novel inhibitor of CAVD by regulating GPR39-dependent ERK1/2 signaling pathway to inhibit cell calcification, and zinc transporter ZIP13 and ZIP14 play important roles in regulating cell calcification and osteogenic differentiation. Zinc supplementation could be a potential therapeutic strategy for CAVD.
CARDIOVASCULAR RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Yuting Ma, Yongshuang Xiao, Zhizhong Xiao, Jun Li
Summary: This study elucidated the nesting structure pattern of Oplegnathus punctatus beak-like tooth and identified four critical periods during early tooth development. The study discovered 11 key genes closely related to tooth development and found their expression in specific tissues. Furthermore, a gene related to calcium accumulation was expressed in the tooth.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Siroshini K. Thiagarajan, Siew Ying Mok, Satoshi Ogawa, Ishwar S. Parhar, Pek Yee Tang
Summary: This study used zebrafish as a biological model to assess the molecular and behavioral changes associated with schizophrenia, opioid addiction, and withdrawal. The findings suggest that there may be common mechanisms underlying schizophrenia-like symptoms and opioid dependence.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Meera Augustus, Donovan Pineau, Franck Aimond, Safa Azar, Davide Lecca, Frederique Scamps, Sophie Muxel, Amelie Darlix, William Ritchie, Catherine Goze, Valerie Rigau, Hugues Duffau, Jean-Philippe Hugnot
Summary: Diffuse grade II IDH-mutant gliomas are rare brain tumors composed of different tumoral cell populations, with little known about these cells. This study identified two distinct tumoral populations, defined reliable markers for them, and uncovered part of the molecular mechanisms generating them. The two cell types have different electrical activity, opening new questions about their role in the pathology.
Article
Environmental Sciences
Karolina Nowak, Ewa Jablonska, Marzena Garley, Agnieszka Iwaniuk, Piotr Radziwon, Slawomir Wolczynski, Wioletta Ratajczak-Wronaa
Summary: This study investigated the estrogen-like effects and mechanism of action of commonly used parabens in human neutrophils. The results showed that parabens did not have cytotoxic effects on neutrophils, nor did they affect neutrophil functions or expression of estrogen receptors.
ENVIRONMENTAL RESEARCH
(2022)
Article
Multidisciplinary Sciences
Dingliang Zhang, Zongyu Gao, He Zhang, Yizhou Yang, Xinxin Yang, Xiaofei Zhao, Hailong Guo, Ugrappa Nagalakshmi, Dawei Li, Savithramma P. Dinesh-Kumar, Yongliang Zhang
Summary: This article investigates a novel MAPK-AL7 module that promotes NLR-mediated immunity by inhibiting the transcription of ROS scavenging genes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Cell Biology
Franka Klatte-Schulz, Kathi Thiele, Markus Scheibel, Georg N. Duda, Britt Wildemann
Summary: The subacromial bursa, previously considered as friction-reducing tissue, is now recognized for its stem cell potential and ability to enhance the regeneration of adjacent tissues. This review explores the role of bursal inflammation as a pain generator and a potential initiator of healing. Therapeutic targets to overcome bursal inflammation and pain are discussed, and further research is needed to fully understand the interplay between the subacromial bursa and rotator cuff tendons.
Article
Biochemistry & Molecular Biology
Manuela Thierbach, Estelle Heyne, Michael Schwarzer, Lauren G. Koch, Steven L. Britton, Britt Wildemann
Summary: The risk of tendon disorders or ruptures increases with age, and intrinsic fitness also plays a role in affecting tendon properties. Low intrinsic fitness accelerates aging of tendons and leads to further impairment, resulting in decreased healing potential and elasticity.
Article
Biochemical Research Methods
Stefan Pentzold, Britt Wildemann
Summary: Mechanical overload decreases tenogenic differentiation and increases extracellular matrix remodeling/inflammation in stimulated fibroblasts, whereas physiological load may induce opposite effects.
JOURNAL OF BIOLOGICAL ENGINEERING
(2022)
Article
Chemistry, Multidisciplinary
Felix Christian Kohler, Philipp Schenk, Paul Koehler, Britt Wildemann, Gunther Olaf Hofmann, Steffen Derlien, Uta Biedermann, Isabel Graul, Jakob Hallbauer
Summary: The direction of PM screw fixation does not affect the primary stability of osteosynthesis of a posterior malleolus fracture. However, anterior to posterior screw fixation may result in slightly greater movement of the fracture. Anterior to posterior screw fixation could be a minimally invasive but stable surgical strategy for posterior malleolus fractures without significant dislocation.
APPLIED SCIENCES-BASEL
(2022)
Review
Cell & Tissue Engineering
G. G. Schulze-Tanzil, M. Delgado-Calcares, R. Stange, B. Wildemann, D. Docheva
Summary: Tendon healing is a major challenge due to its poor blood supply and slow metabolic rate. The disruption of cell-extracellular matrix interactions and uncontrolled inflammation can hinder successful healing. Optimizing Achilles tendon (AT) healing outcomes may involve adapting mechanical stimulation, restricting neoangiogenesis, and modifying stem cell niche parameters.
BONE & JOINT RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Hui Zhang, Nirina Beilfuss, Urszula Zabarylo, Kay Raum, Regina Puts
Summary: In order to rapidly develop precision medicine and patient-oriented treatment strategies, novel technological solutions are urgently needed. This study developed an ultrasound-based tissue engineering acoustophoretic set-up to organize murine mesenchymal stromal cells in an in situ polymerizing 3-D fibrin hydrogel. The structured hydrogels exhibited enhanced expression of osteogenic markers and cell-cycle-progression-associated genes, suggesting the potential for improved regenerative properties in bone healing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
T. Fintan Moriarty, Willem-Jan Metsemakers, Mario Morgenstern, Marloes Hofstee, Alejandro Vallejo Diaz, James E. Cassat, Britt Wildemann, Melissa Depypere, Edward M. Schwarz, R. Geoff Richards
Summary: Fracture-related infection (FRI) is a common complication of bone fractures, where pathogenic bacteria enter the wound and hinder the healing process. This Primer provides an overview of the epidemiology, pathobiology, diagnosis, and management of these infections, as well as their impact on patient quality of life.
NATURE REVIEWS DISEASE PRIMERS
(2022)
Article
Medicine, General & Internal
Felix Christian Kohler, Philipp Schenk, Theresa Nies, Jakob Hallbauer, Gunther Olaf Hofmann, Uta Biedermann, Heike Kielstein, Britt Wildemann, Roland Ramm, Bernhard Wilhelm Ullrich
Summary: In the treatment of ankle fractures, complications related to wound healing after open reduction and internal fixation are a significant issue. A minimally invasive nail stabilization technique has been proposed as an alternative. This biomechanical study aimed to determine if this technique is comparable to the established method. The results showed that both the nail and plate fixations resulted in reduced stability and rotational stiffness compared to the intact ankle. While the nail fixation had greater rotational stiffness, both methods fell short of the native stability. The study suggests that weight-bearing after nail fixation should be evaluated on a case-by-case basis.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Shibarjun Mandal, Astrid Tannert, Christina Ebert, Rustam R. Guliev, Yvonne Ozegowski, Lina Carvalho, Britt Wildemann, Simone Eiserloh, Sina M. Coldewey, Bettina Loeffler, Luis Bastiao Silva, Verena Hoerr, Lorena Tuchscherr, Ute Neugebauer
Summary: Osteomyelitis, a difficult-to-treat bone infection caused by Staphylococcus aureus, was studied using mouse models. Fluorescence imaging and label-free Raman spectroscopy were used to characterize tissue changes and bacterial localization. The results showed that bacteria formed abscesses in the infected tissue, with lower numbers found in surrounding muscle tissue and trabecular bone tissue. Raman spectroscopic imaging revealed a metabolic state of the bacteria with reduced activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Manuela Thierbach, Michelle Mueller, Richard Stange, Daniel Kronenberg, Matthias Aurich, Britt Wildemann
Summary: Although prophylactic use of vancomycin is routinely performed after ACL reconstruction, it may potentially harm the tissue and cells. A comprehensive study was conducted to investigate its effect on tendon tissue and isolated tenocytes, and the results demonstrated that vancomycin at clinically used concentrations had no negative impact on cell viability, gene expression, histology, and mechanical integrity.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Engineering, Biomedical
Nicole Bormann, Aysha Schmock, Anja Hanke, Volker Eras, Norus Ahmed, Maya S. Kissner, Britt Wildemann, Jan C. Brune
Summary: Bone defects and infections present challenges for treatment, and this study evaluated the efficacy of different bone allografts in antibiotic absorption and release. The fibrous grafts showed the highest absorption capacity, while mineralized cancellous bone had the lowest absorption capacity. The fibrous grafts also exhibited prolonged antibiotic release and activity, making them suitable carrier grafts for antibiotic administration.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Medicine, General & Internal
Jakob Hallbauer, Philipp Schenk, Lea Herrmann, Bernhard Wilhelm Ullrich, Uta Biedermann, Britt Wildemann, Gunther Olaf Hofmann, Felix Christian Kohler
Summary: The hook test is commonly used to assess syndesmosis stability during surgery, but there are no guidelines for the force required or the reliability of the test. In this study, ten experienced surgeons performed hook tests on cadaver bone models, with forces recorded in a blinded manner. Standardized hook tests were also performed on cadaver lower legs, revealing that the force and instability of the syndesmosis significantly influenced syndesmosis diastasis. However, even with maximum instability, only a small percentage of specimens showed significant diastasis (>2 mm). The hook test shows high variability and is not able to detect relevant syndesmosis injuries, even when performed in a standardized manner.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Medicine, General & Internal
Stefan Ferdinand Hertling, David Alexander Back, Britt Wildemann, Ekkehard Schleussner, Mario Kaiser, Isabel Graul
Summary: Mentoring programs have a positive impact on students' career planning, but the participation of surgical specialties is low. Increasing the availability of mentoring programs, with a special focus on women and more surgical content, can help address the shortage of surgical trainees.
FRONTIERS IN MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Runa Kinitz, Estelle Heyne, Lauren G. Koch, Steven L. Britton, Manuela Thierbach, Britt Wildemann
Summary: Old age, adiposity, and metabolic disorders are risk factors for chronic tendinopathy, and aging seems to play a prominent role in molecular and structural alterations of Achilles tendon tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)