Article
Cardiac & Cardiovascular Systems
Maria Raquel Moita, Marta M. Silva, Claudia Diniz, Margarida Serra, Rene M. Hoet, Ana Barbas, Daniel Simao
Summary: This study investigates the role of cardiac fibroblasts in cardiac fibrosis and identifies potential targets for anti-fibrotic therapies. The researchers also demonstrate the suitability of induced pluripotent stem cells as a cell source for preclinical research.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Immunology
Yuan You, Patrick Stelzl, Dana N. Joseph, Paulomi B. Aldo, Anthony J. Maxwell, Nava Dekel, Aihua Liao, Shannon Whirledge, Gil Mor
Summary: In this study, a 3D in vitro model was used to investigate the interaction between trophoblasts and human endometrial stroma cells, demonstrating how stromal factors promote trophoblast invasion in an inflammatory environment modulated by TNF-alpha. Additionally, stromal-derived IL-17 was found to play a role in promoting trophoblast migration by inducing essential genes for invasive capacity. These findings provide new insights into the complex process of implantation and highlight the importance of inflammation in embryo implantation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemical Research Methods
Maria Ryaboshapkina, Kevin Saitoski, Ghaith M. Hamza, Andrew F. Jarnuczak, Severine Pechberty, Claire Berthault, Kaushik Sengupta, Christina Rye Underwood, Shalini Andersson, Raphael Scharfmann
Summary: In this study, the representativeness of EndoC-beta H1 cells as a translational human beta cell model was verified through omics analysis. The secretome of EndoC-beta H1 cells was characterized, revealing similarities and differences compared to human beta cells and insulinomas. The study also identified a high proportion of extracellular vesicle proteins in the secretome of EndoC-beta H1 cells. These findings provide valuable insights into the secretion regulation of human beta cells and warrant further investigation.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Cell Biology
Wayne Carver, Ethan Fix, Charity Fix, Daping Fan, Mrinmay Chakrabarti, Mohamad Azhar
Summary: Studies have shown that emodin can attenuate TGF-beta 1-induced cardiac fibroblast activation and collagen accumulation, and it may function as an effective therapeutic agent for fibrosis through specific TGF-beta signaling pathways.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Genetics & Heredity
Friederike Kirschner, Danielle Arnold-Schild, Christian Leps, Mateusz Krzysztof Lacki, Matthias Klein, Yannic Chen, Annekathrin Ludt, Federico Marini, Can Kucuk, Lara Stein, Ute Distler, Malte Sielaff, Thomas Michna, Kristina Riegel, Krishnaraj Rajalingam, Tobias Bopp, Stefan Tenzer, Hansjoerg Schild
Summary: The analysis of secretome provides important information on intercellular communication and cell behavior in specific tissues. Especially in tumors, secretome data can aid in diagnosis and therapy decisions. Mass spectrometry-based analysis of cell-conditioned media is commonly used for unbiased characterization of cancer secretomes in vitro. Metabolic labeling using azide-containing amino acid analogs facilitates this analysis in the presence of serum. However, these modified analogs may affect protein folding and incorporation into newly synthesized proteins. Combining transcriptome and proteome analysis, we investigate the effects of azidohomoalanine labeling on gene and protein expression. Our findings indicate that AHA labeling induces changes in transcript and protein expression, leading to cellular stress, apoptosis-related pathways, and alteration of secretome composition on a global scale.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2023)
Article
Medicine, Research & Experimental
Shih-Yi Lee, Yueh-Hsiung Kuo, Chen-Xuan Du, Cheng-Wei Huang, Hui-Chun Ku
Summary: Differentiation of cardiac fibroblasts into myofibroblasts is a critical event in the progression of cardiac fibrosis. The study investigated the effect of caffeic acid ethanolamide (CAEA) on cardiac remodeling induced by Angiotensin (Ang) II. CAEA inhibited fibroblast differentiation, protected against cardiac fibrosis and dysfunction, and regulated the TGF-beta/SMAD/NOX4 signaling pathway. The study supports CAEA administration as a viable method to prevent Ang II-induced cardiac remodeling.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Biotechnology & Applied Microbiology
Jiri Vorel, Krystyna Cwiklinski, Pavel Roudnicky, Jana Ilgova, Lucie Jedlickova, John P. Dalton, Libor Mikes, Milan Gelnar, Martin Kasny
Summary: This study presented a comprehensive transcriptomic/secretome description of Eudiplozoon nipponicum, shedding light on the physiological pathways and protein molecules involved in host-parasite interactions. By integrating high-throughput sequencing techniques, mass spectrometry analysis, and bioinformatics, the study provided a better understanding of the molecular biology and biochemistry of these often neglected organisms. The research identified numerous transcripts and proteins responsible for immunomodulation, feeding, and development, offering valuable insights into the biology of Diplozoidae ectoparasites.
Article
Pharmacology & Pharmacy
Xiaokang Luo, Shiyang Song, Lei Qi, Chih-Liang Tien, Hui Li, Weiyi Xu, Theodore Lemuel Mathuram, Thomas Burris, Yuanbiao Zhao, Zheng Sun, Lilei Zhang
Summary: This study characterized the functional difference of REV-ERB in mouse embryonic fibroblasts and cardiac fibroblasts. The results demonstrated the essential cell-autonomous role of REV-ERB alpha/beta in maintaining cardiac fibroblasts in a healthy, quiescent state.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Jin-xing Zhu, Wang Ling, Chao Xue, Zhen Zhou, Yi-shuai Zhang, Chen Yan, Mei-ping Wu
Summary: The study aimed to investigate the impact of Higenamine (HG) on chronic cardiac remodeling, particularly cardiac fibrosis. The results showed that HG could alleviate chronic cardiac hypertrophy, fibrosis, and dysfunction by suppressing TGF-β1/Smad signaling and the activation of cardiac fibroblasts (CFs).
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Review
Cell Biology
Laxmansa C. Katwa, Chelsea Mendoza, Madison Clements
Summary: Cardiovascular disease is the leading cause of death worldwide, and patients with cardiovascular diseases are more susceptible to severe complications of COVID-19. The role of cardiac myofibroblasts in both COVID-19-induced cardiac injury and healing process is crucial.
Review
Biochemistry & Molecular Biology
Thanachai Methatham, Ryozo Nagai, Kenichi Aizawa
Summary: The accumulation of fibrosis in cardiac tissues is a major cause of heart failure, with activated fibroblasts and myofibroblasts playing a key role. TGF-beta signaling pathways and the control of TGF-beta by KLF5 have been found to be important in cardiac fibrosis. Understanding metabolic alterations, particularly in glycolysis, is essential in explaining fibrosis. However, the interplay among TGF-beta, KLF5, and glycolysis in fibrosis pathogenesis is still unclear.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Christophe Magaud, Thomas Harnois, Stephane Sebille, Aurelien Chatelier, Jean-Francois Faivre, Patrick Bois, Guylene Page, Barnabas Gellen
Summary: This study investigates the internalization of TTR aggregates in cardiac fibroblasts and their effects on the secretion of inflammatory factors. The results show that TTR aggregates can induce the secretion of Brain Natriuretic Peptide (BNP), N-terminal pro B-type Natriuretic Peptide (NT-proBNP), interleukin-6 (IL-6), and interleukin-8 (IL-8) by cardiac fibroblasts. However, the levels of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) remain unchanged. These findings suggest that IL-6 and IL-8 play important roles in the development of ATTR-wt and could be potential therapeutic targets.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biotechnology & Applied Microbiology
Larken Root, Aurora Campo, Leah MacNiven, Pazit Con, Avner Cnaani, Dietmar Kultz
Summary: This study generated a DIA assay library for targeted quantitation of thousands of Oreochromis niloticus gill proteins, comparing protein and mRNA abundances in freshwater and brackish water environments. The results highlighted the non-linearity in the regulation of specific functional groups by salinity, suggesting post-transcriptional mechanisms play a role in the salinity-dependent regulation of gill function in O. niloticus.
Article
Engineering, Biomedical
Tom C. L. Bracco Gartner, Ye Wang, Laurynas Leiteris, Iris van Adrichem, Judith Marsman, Marie Jose Goumans, Carlijn V. C. Bouten, Joost P. G. Sluijter, Jaap M. J. den Toonder, Willem J. L. Suyker, Jesper Hjortnaes
Summary: Cardiac fibroblasts in the ever-beating human heart remain quiescent due to the antifibrotic effect of cyclic strain conditions revealed by a novel platform for studying cardiac fibrosis-on-a-chip. This study provides insights into the mechanosensitive pathways and genes involved in the fibrogenic process, which can contribute to the development of new therapies against cardiac fibrosis.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2023)
Article
Medicine, Research & Experimental
Jian-Chun Li, Jian Jia, Li Dong, Zhong-Jing Hu, Xiao-Ru Huang, Hong-Lian Wang, Li Wang, Si-Jin Yang, Hui-Yao Lan
Summary: This study investigated the role of Erbb4-IR in hypertensive heart disease using ultrasound-microbubble-mediated silencing. It was found that Erbb4-IR mediates cardiac fibrosis induced by Ang II, and silencing Erbb4-IR effectively improves cardiac dysfunction and fibrosis. Therefore, targeting Erbb4-IR may be a novel therapy for hypertensive cardiovascular diseases.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Article
Cardiac & Cardiovascular Systems
Kishore Surendra, Sylvia Nuernberg, Jan P. Bremer, Marius S. Knorr, Frank Ueckert, Jan Per Wenzel, Ramona Bei Der Kellen, Dirk Westermann, Renate B. Schnabel, Raphael Twerenbold, Christina Magnussen, Paulus Kirchhof, Stefan Blankenberg, Johannes Neumann, Benedikt Schrage
Summary: The study aims to develop a practical screening tool for heart failure at the general population level. By using a convolutional neural network (CNN) built on digital electrocardiograms (ECGs), the researchers achieved comparable predictive accuracy for heart failure as the logistic regression model and the random forest classifier. The study suggests that this screening tool could be integrated into the clinical routine of general practitioners, alleviating the underdiagnosis of heart failure.
Article
Cardiac & Cardiovascular Systems
Shinwan Kany, Christoph Al-Taie, Carolina Roselli, James P. Pirruccello, Katrin Borof, Carla Reinbold, Anna Suling, Linda Krause, Bruno Reissmann, Renate B. Schnabel, Tanja Zeller, Antonia Zapf, Karl Wegscheider, Larissa Fabritz, Patrick T. Ellinor, Paulus Kirchhof
Summary: The Early Treatment of Atrial Fibrillation for Stroke Prevention Trial has shown that early rhythm control is effective in reducing cardiovascular events in patients with atrial fibrillation (AF). However, the interaction between genetic predisposition to AF and stroke and early rhythm control therapy is still unknown.
CARDIOVASCULAR RESEARCH
(2023)
Article
Cardiac & Cardiovascular Systems
Carl Schulz, Marc D. Lemoine, Giulia Mearini, Jussi Koivumaeki, Jascha Sani, Edzard Schwedhelm, Paulus Kirchhof, Amer Ghalawinji, Monika Stoll, Arne Hansen, Thomas Eschenhagen, Torsten Christ
Summary: This study found that deletion of the PITX2 gene in human induced pluripotent stem cell-derived atrial cardiomyocytes results in some abnormalities similar to electrical remodeling in atrial fibrillation. These findings suggest that the PITX2 gene plays an important role in the occurrence of atrial fibrillation.
CIRCULATION-ARRHYTHMIA AND ELECTROPHYSIOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Jannis Dickow, Shinwan Kany, Victor Roth Cardoso, Patrick T. Ellinor, Georgios V. Gkoutos, Holly K. Van Houten, Paulus Kirchhof, Andreas Metzner, Peter A. Noseworthy, Xiaoxi Yao, Andreas Rillig
Summary: A recent study suggests that early rhythm control is more beneficial for patients with atrial fibrillation and a high comorbidity burden. This study analyzed a large database and found that early rhythm control can reduce the risk of death, stroke, heart failure, or myocardial infarction in patients with a high comorbidity burden.
CIRCULATION-ARRHYTHMIA AND ELECTROPHYSIOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Sophie Gottschalk, Shinwan Kany, Hans-Helmut Koenig, Harry J. G. M. Crijns, Panos Vardas, A. John Camm, Karl Wegscheider, Andreas Metzner, Andreas Rillig, Paulus Kirchhof, Judith Dams
Summary: The EAST-AFNET 4 trial found that early rhythm control (ERC) reduces the rate of a composite primary outcome by around 20%. This study investigated the cost-effectiveness of ERC compared to usual care and concluded that, from a German healthcare payer's perspective, health benefits of ERC may come at reasonable costs.
Editorial Material
Cardiac & Cardiovascular Systems
Andreas Metzner, Paulus Kirchhof
EUROPEAN HEART JOURNAL
(2023)
Article
Cell Biology
Theresa Holst, Johannes Petersen, Sabine Ameling, Lisa Mueller, Torsten Christ, Naomi Gedeon, Thomas Eschenhagen, Hermann Reichenspurner, Elke Hammer, Evaldas Girdauskas
Summary: This study identified specific left ventricular (LV) myocardial protein signatures that contribute to the differential disease progression in patients with chronic aortic regurgitation (AR) and aortic stenosis (AS) after aortic valve (AV) surgery.
Article
Cardiac & Cardiovascular Systems
Renate B. Schnabel, Pietro Ameri, Jolanta M. Siller-Matula, Igor Diemberger, Marianne Gwechenberger, Ladislav Pecen, Marius Constantin Manu, Jose Souza, Raffaele De Caterina, Paulus Kirchhof
Summary: Heart failure is a risk factor for adverse events in patients with atrial fibrillation. This study investigates the relationship between heart failure and outcomes in atrial fibrillation patients treated with edoxaban. The results show that patients with heart failure have higher rates of major bleeding and all-cause death, indicating the need for optimized management and novel preventive strategies.
Article
Biochemistry & Molecular Biology
Simone Venz, Viola von Bohlen Und Halbach, Christian Hentschker, Heike Junker, Andreas Walter Kuss, Thomas Sura, Elke Krueger, Uwe Voelker, Oliver von Bohlen Und Halbach, Lars Riff Jensen, Elke Hammer
Summary: A workflow for tandem mass spectrometry-based protein profiling of immunohistochemistry specimens was established and applied to tissue sections. The CA1 region of the hippocampus was investigated using protein extraction and digestion methods followed by mass spectrometry measurement and data analysis. Literature searches and Gene Ontology annotation verified the applicability of this method for global protein profiling using formalin-fixed and embedded material and previously used immunohistochemistry slides.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Alexander Reder, Christian Hentschker, Leif Steil, Manuela Gesell Salazar, Elke Hammer, Vishnu M. Dhople, Thomas Sura, Ulrike Lissner, Hannes Wolfgramm, Denise Dittmar, Marco Harms, Kristin Surmann, Uwe Voelker, Stephan Michalik
Summary: Fast, efficient, and reproducible sample preparation is crucial in proteomics research. A web application called MassSpecPreppy, operating on the OT-2 liquid handling robot, has been developed to address this need. This platform allows for the automated and flexible preparation of up to 96 samples, including tasks such as protein concentration determination, sample digestion, and peptide elution, producing injection-ready samples.
Article
Cardiac & Cardiovascular Systems
Moritz Seiffert, Reinhard Vonthein, Helmut Baumgartner, Michael A. Borger, Yeong-Hoon Choi, Volkmar Falk, Norbert Frey, Andreas Hagendorff, Christian Hagl, Christian Hamm, Inke R. Koenig, Ulf Landmesser, Steffen Massberg, Hermann Reichenspurner, Holger Thiele, Raphael Twerenbold, Maren Vens, Thomas Walther, Andreas Ziegler, Jochen Cremer, Stefan Blankenberg
Summary: TAVI has become the preferred treatment option for severe aortic stenosis, but the optimal treatment strategy for younger, low-risk patients remains controversial. The DEDICATE-DZHK6 Trial aims to investigate the efficacy and safety of TAVI compared to SAVR in low-to intermediate-risk patients aged 65 years or older, and provide important information for clinical practice.
Article
Cardiac & Cardiovascular Systems
Constanze Volgmann, Markus J. Barten, Yousuf Al Assar, Hanno Grahn, Andreas Metzner, Gerold Soeffker, Leonie Schulte-Uentrop, Christina Magnussen, Paulus Kirchhof, Stefan Kluge, Susanne Doll, Nicolas Doll, Hermann Reichenspurner, Alexander M. Bernhardt
Summary: This article presents a case where the surgically implanted Impella 5.5 was used for multiple indications and treatment strategies in one patient. The patient underwent urgent coronary bypass grafting followed by unsuccessful heart recovery and recurrent ventricular tachycardia. After evaluation, the patient received a heart transplant with successful surgical placement of an Impella 5.5.
EUROPEAN HEART JOURNAL-CASE REPORTS
(2023)
Article
Clinical Neurology
Maerit Jensen, Rustam Al-Shahi Salman, G. Andre Ng, H. Bart van der Worp, Peter Loh, Bruce C. V. Campbell, Jonathan M. Kalman, Michael D. Hill, Luciano A. Sposato, Jason G. Andrade, Andreas Metzner, Paulus Kirchhof, Goetz Thomalla
Summary: Despite the potential benefit of early rhythm control in patients with acute ischemic stroke and AF, it appears that only a small percentage of these patients receive rhythm control treatment, possibly due to concerns about potential complications. A randomized clinical trial is needed to assess the efficacy of early rhythm control in this patient population.
NEUROLOGICAL RESEARCH AND PRACTICE
(2023)
Article
Clinical Neurology
Thies Ingwersen, Manuel C. Olma, Eckhard Schlemm, Carola Mayer, Bastian Cheng, Serdar Tuetuencue, Paulus Kirchhof, Roland Veltkamp, Joachim Roether, Ulrich Laufs, Darius G. Nabavi, George Ntaios, Matthias Endres, Karl Georg Haeusler, Goetz Thomalla
Summary: This study externally validated the ESUS recurrence score and supported a threshold of >= 7 for identifying ESUS patients at high-risk of stroke recurrence. However, further adjustments may be needed to improve the model's performance in independent cohorts. The use of risk scores may be helpful in guiding extended diagnostics and further trials on secondary prevention in patients with ESUS.
NEUROLOGICAL RESEARCH AND PRACTICE
(2023)
Review
Cardiac & Cardiovascular Systems
Hanna Braeuninger, Saskia Krueger, Lucas Bacmeister, Alexander Nystroem, Kilian Eyerich, Dirk Westermann, Diana Lindner
Summary: Cardiovascular diseases (CVDs) continue to be the leading cause of death worldwide, with ischaemic heart diseases like myocardial infarction (MI) accounting for most deaths. Atherosclerosis in the coronary arteries often precedes the manifestation of the disease. Regulation of matrix metalloproteinases (MMPs) is crucial in the development and progression of atherosclerosis and the outcome of MI. This review aims to provide an overview of MMP expression in coronary artery and left ventricular tissue, as well as the impact of MMP gene polymorphisms on CVD development and outcome.
BASIC RESEARCH IN CARDIOLOGY
(2023)