Journal
RSC ADVANCES
Volume 6, Issue 101, Pages 99540-99545Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6ra18430h
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Funding
- National Natural Science Foundation of China [31271860]
- Program for New Century Excellent Talents in University [NCET-12-0878]
- Fundamental Research Funds for the Central Universities [JUSRP51402A]
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Graphene oxide (GO) was introduced in the process of systematic evolution of ligands by exponential enrichment (SELEX) to screen ofloxacin-specific single-stranded DNA aptamers. Four sequences with high homology, ap1, ap3, ap4 and ap5 were picked out. Their secondary structures were simulated, and the dissociation constants (K-d) were measured, which were 251.3, 130.1, 159.1 and 304.4 nM, respectively. The specificity of the aptamers was also evaluated.
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