4.6 Article

Graphene oxide-based selection and identification of ofloxacin-specific single-stranded DNA aptamers

Journal

RSC ADVANCES
Volume 6, Issue 101, Pages 99540-99545

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c6ra18430h

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Funding

  1. National Natural Science Foundation of China [31271860]
  2. Program for New Century Excellent Talents in University [NCET-12-0878]
  3. Fundamental Research Funds for the Central Universities [JUSRP51402A]

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Graphene oxide (GO) was introduced in the process of systematic evolution of ligands by exponential enrichment (SELEX) to screen ofloxacin-specific single-stranded DNA aptamers. Four sequences with high homology, ap1, ap3, ap4 and ap5 were picked out. Their secondary structures were simulated, and the dissociation constants (K-d) were measured, which were 251.3, 130.1, 159.1 and 304.4 nM, respectively. The specificity of the aptamers was also evaluated.

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