Article
Endocrinology & Metabolism
Peng Zhou, Chao Yang, Shan Zhang, Zun-Xiang Ke, Dian-Xi Chen, Yi-Qing Li, Qin Li
Summary: The importance of early diagnosis and treatment of diabetes and its cutaneous complications is increasingly recognized. High glucose affects collagen gene expression in human dermal fibroblasts, leading to an imbalance of matrix metalloproteinases and tissue inhibitors of metalloproteinases. Inhibiting MMP-2 and MMP-9 can alleviate collagen deposition disorder in the skin of diabetic mice.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Kaja Michalczyk, Aneta Cymbaluk-Ploska
Summary: Matrix metalloproteinases (MMPs) play a crucial role in the development and progression of endometrial cancer, with significantly elevated levels in cancer patients compared to normal individuals. Understanding the role of MMPs may have implications for diagnosis and treatment of endometrial cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Arno Kirchhain, Asta Zubriene, Visvaldas Kairys, Federico Vivaldi, Andrea Bonini, Denise Biagini, Delio Santalucia, Daumantas Matulis, Fabio Di Francesco
Summary: Matrix metalloproteinases play a crucial role in cancer progression and metastasis, and molecular probes have been successfully used to study their activity. In this study, a series of MMP-2 and MMP-9 binding probes were synthesized, showing high selectivity against MMP-2 and potential for sensor fabrication. The experimental results were confirmed by molecular modeling, indicating the potential application of these probes for targeted enzyme detection.
BIOORGANIC CHEMISTRY
(2021)
Article
Gastroenterology & Hepatology
Lorand Barabas, Istvan Hritz, Gabor Istvan, Zsolt Tulassay, Laszloo Herszenyi
Summary: The serum levels of MMP-7, MMP-9, TIMP-1, and TIMP-2 were significantly elevated in patients with CRC and adenomas compared to controls, indicating their potential role in CRC invasion and possible prognostic impact. These findings suggest that MMPs and their inhibitors play a crucial role in CRC progression.
DIGESTIVE DISEASES
(2021)
Article
Agriculture, Dairy & Animal Science
Juanjuan Song, Ligang Yuan, Hua Wang, Guojuan Chen, Shaoyu Chen
Summary: This study investigated the distribution and expression of MMP-2 and TIMP-2 in yak testes. The results showed that MMP-2 and TIMP-2 were mainly located in different cell types at different ages. The expression levels of MMP-2 and TIMP-2 varied with age, with a decrease from newborn to adult and an increase in old yaks. This study revealed the possible roles of MMP-2 and TIMP-2 in testicular functionality of yaks at different ages.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Medicine, Research & Experimental
Carolina D'Avila-Mesquita, Ariel E. S. Couto, Ligia C. B. Campos, Tauana F. Vasconcelos, Jessyca Michelon-Barbosa, Carlos A. C. Corsi, Fabiola Mestriner, Bruno C. Petroski-Moraes, Maria J. Garbellini-Diab, Daniel M. S. Couto, Maria C. Jordani, Denise Ferro, Lourenco Sbragia, Edwaldo E. Joviliano, Paulo R. Evora, Rodrigo de Carvalho Santana, Olindo Assis Martins-Filho, Katarzyna Polonis, Mayra G. Menegueti, Mauricio S. Ribeiro, Maria Auxiliadora-Martins, Christiane Becari
Summary: The study revealed that plasma levels of MMP-2 and MMP-9 in COVID-19 patients were associated with clinical outcomes and mortality. MMP-9 levels in COVID-19 patients were influenced by comorbidities such as hypertension and obesity, while MMP-2 levels were affected by hypertension.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Sasha Coates-Park, Carolyn Lazaroff, Sadeechya Gurung, Josh Rich, Alexandra Colladay, Maura O'Neill, Georgina S. Butler, Christopher M. Overall, William G. Stetler-Stevenson, David Peeney
Summary: Extracellular proteolysis and turnover play crucial roles in tissue homeostasis. MMPs, as the main matrix-degrading enzymes, are regulated by TIMPs. The balance between MMP activity and TIMP levels is essential for healthy tissue homeostasis and can be disrupted in pathological conditions.
Article
Biotechnology & Applied Microbiology
Luo Qiong, Jun Yin
Summary: The study shows that the upregulated expression of ORM1 in breast cancer tissues and cell lines enhances cell proliferation and migration. In contrast, down-regulation of ORM1 inhibits the expression of MMP-2 and MMP-9, as well as the activation of the AKT/ERK signaling pathway. These findings suggest that ORM1 may be a potential therapeutic target for breast cancer and play a role in promoting epirubicin resistance.
Article
Biochemistry & Molecular Biology
Joana Mota, Rosa Direito, Joao Rocha, Joao Fernandes, Bruno Sepodes, Maria Eduardo Figueira, Anabela Raymundo, Ana Lima, Ricardo Boavida Ferreira
Summary: The study isolated and characterized an MMP-2 and -9 inhibitor from Lupinus albus, showing its efficacy in inhibiting MMP-9 activity in colon cancer cells and significantly reducing colitis symptoms in a mouse model. This novel type of MMP inhibitor in lupine, edible and proteinaceous in nature, holds high potential for application as a nutraceutical or functional food targeting pathologies related to high MMP-9 activity in the digestive system.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jacek Kudelski, Anna Tokarzewicz, Monika Gudowska-Sawczuk, Barbara Mroczko, Piotr Chlosta, Marta Bruczko-Goralewska, Przemyslaw Mitura, Grzegorz Mlynarczyk
Summary: This study aimed to evaluate the expression and activity of matrix metalloproteinase 2 and 9 (MMP-2 and MMP-9) in urinary bladder cancer at different stages. The results showed that the content and activity of MMP-9 were higher than MMP-2, especially in high-grade cancer tissue. MMP-9 may have higher clinical utility as a potential therapeutic option and a diagnostic biomarker for urinary bladder cancer.
Article
Infectious Diseases
Bastian Lubis, Aznan Lelo, Putri Amelia, Agus Prima
Summary: This study was a randomized controlled trial examining the effects of vitamin C and thiamine in septic patients. The study found that thiamine alone provided better advantages than either vitamin C alone or their combination.
INFECTION AND DRUG RESISTANCE
(2022)
Review
Oncology
Chenglu Huang, Suqin Xu, Zhilin Luo, Dong Li, Rui Wang, Tianhu Wang
Summary: This meta-analysis evaluated the association between variants in MMP-2, MMP-7, and MMP-9 genes and the risk of various cancers. The findings suggest a significant association between these variants and cancer risk, especially in Asians.
FRONTIERS IN ONCOLOGY
(2022)
Review
Pharmacology & Pharmacy
Kang Fu, Xueyao Zheng, Yuhan Chen, Liuying Wu, Zhiming Yang, Xu Chen, Wei Song
Summary: Diabetic foot ulcers (DFUs) are serious complications of diabetes mellitus, characterized by tissue destruction in the foot. Matrix metalloproteinases (MMPs) play crucial roles in the pathogenesis and wound healing process of DFUs. Overexpression of MMPs in DFUs can result in excessive tissue degradation and impaired wound healing. Regulation of MMP levels may facilitate wound healing in DFUs.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Zainab Ahmed Rashid, Sanaa K. Bardaweel
Summary: Matrix metalloproteinases (MMPs), especially MMP-9, play a significant role in cancer pathogenesis and progression. However, no effective MMP-9 inhibitor has been approved by the FDA. This review focuses on exploring the strategies for designing MMP-9 inhibitors and their anticancer effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Barbara Maria Piskor, Andrzej Przylipiak, Emilia Dabrowska, Iwona Sidorkiewicz, Marek Niczyporuk, Maciej Szmitkowski, Slawomir Lawicki
Summary: The study evaluated the plasma levels of MMP-7, MMP-26, and CA 15-3 in breast cancer patients and found that a combination of these markers showed potential for improving diagnostic accuracy. MMP-7, MMP-26, and CA 15-3 levels were associated with disease progression in breast cancer patients, with the combination of markers showing high sensitivity and specificity, particularly in advanced stages of the disease. Further research is needed to confirm the potential of matrilysins as markers for breast cancer diagnosis.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Medicine, Research & Experimental
Marzena Garley, Dorota Dziemianczyk-Pakiela, Wioletta Ratajczak-Wrona, Anna Pryczynicz, Karolina Nowak, Bogdan Lazarczyk, Ewa Jablonska
Summary: The study aimed to determine the clinical usefulness of NETs biomarkers in saliva as a potential prognostic and therapeutic target in patients with oral squamous cell carcinoma (OSCC). Results showed significantly lower concentrations of NOX1, NCF2, and DNASE1 in the saliva samples of cancer patients, suggesting an impairment of immunological homeostasis within the oral cavity.
ADVANCES IN MEDICAL SCIENCES
(2022)
Article
Environmental Sciences
Justyna Wasielica-Berger, Pawel Rogalski, Agnieszka Swidnicka-Siergiejko, Anna Pryczynicz, Joanna Kisluk, Jaroslaw Daniluk, Stefania Antonowicz, Dominik Maslach, Michalina Krzyzak, Andrzej Dabrowski
Summary: This study found a correlation between the expression of growth factors and endoscopic findings, formation of endoscopic minimal changes, and histologic lesions in patients with GERD.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Medicine, General & Internal
Aleksandra Czajkowska, Katarzyna Guzinska-Ustymowicz, Anna Pryczynicz, Dariusz Lebensztejn, Urszula Daniluk
Summary: This study aimed to investigate the relationship between MMP-9 and TIMP-1 and UC phenotypes, disease activity index, and inflammatory markers. The results showed that children with UC had significantly higher levels of these markers in their serum and feces compared to the control group. Additionally, serum MMP-9 correlated with the severity of the disease and other markers, while serum TIMP-1 correlated with disease activity, inflammatory markers, and erythrocyte sedimentation rate. Serum and fecal levels of MMP-9 and TIMP-1 can be useful in distinguishing UC patients and evaluating disease phenotypes.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Diana Sawicka, Agnieszka Hryniewicka, Sylwia Gohal, Anna Sadowska, Anna Pryczynicz, Katarzyna Guzinska-Ustymowicz, Emilia Sokolowska, Jacek W. Morzycki, Halina Car
Summary: This research evaluated a series of novel imidazolium salts substituted with lithocholic acid and alkyl chains of various lengths against colon cancer cells. The results showed that these salts have anticancer activity, which is related to the length of the alkyl chain. The study also demonstrated the inhibitory effect of one compound on tumor growth in a mouse model.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Anna Sadowska, Diana Sawicka, Katarzyna Godlewska, Katarzyna Guzinska-Ustymowicz, Ewa Zapora, Emilia Sokolowska, Halina Car
Summary: Fungal extract from Heterobasidion annosum (HA) has been evaluated for its potential anticancer activity in colorectal cancer. In an in vivo model, mice with implanted DLD-1 human cancer cells were treated with HA extract, the drug 5-fluorouracil (5FU), or a combination of both. Results showed that the combination treatment led to the smallest tumor volumes, with increased concentrations of apoptotic proteins caspase-8 and p53, and decreased levels of survivin and Bcl-2. The Ki-67 protein was found to be expressed in all tested groups. These findings provide innovative insights into the antitumor activity of HA extract and its interaction with chemotherapeutic agents.
Article
Multidisciplinary Sciences
Adam R. Markowski, Anna J. Markowska, Wiktoria Ustymowicz, Anna Pryczynicz, Katarzyna Guzinska-Ustymowicz
Summary: This study conducted a simultaneous analysis of tumor-infiltrating immune cell density, presence of lymphoid follicles, and budding status in colorectal cancer tissue for the first time. The results showed that tumor budding, lymphocyte infiltration, and lymphoid follicles could serve as reliable indicators of colorectal cancer progression.
SCIENTIFIC REPORTS
(2022)
Article
Medicine, Research & Experimental
Mariusz Gryko, Marta Lukaszewicz-Zajac, Katarzyna Guzinska-Ustymowicz, Mariola Kucharewicz, Barbara Mroczko, Utkus Algirdas
Summary: The aim of this study was to assess the expression of caspase-8 and procaspase-3 proteins in gastric cancer cells and non-cancerous mucosa, and its relationship with clinical and morphological characteristics of the tumor, postoperative survival, and other apoptosis-related proteins. The results showed that procaspase-3 was expressed in all gastric cancer cells, with a significant difference in expression compared to non-cancerous mucosa. Caspase-8 expression was more common in certain types of gastric cancer and was associated with longer survival and better prognosis.
ADVANCES IN MEDICAL SCIENCES
(2023)
Article
Oncology
Adam R. Markowski, Konstancja Ustymowicz, Anna J. Markowska, Wiktoria Romanczyk, Katarzyna Guzinska-Ustymowicz
Summary: Different distributions of E-cadherin expression were observed within colorectal cancer, with the highest percentage of positive expression in the invasive front and tumor center. The expression pattern varied depending on cancer progression and other clinical and histopathological features. Low E-cadherin expression appears to be a reliable indicator of higher cancer staging and progression.
Article
Gastroenterology & Hepatology
Justyna Wasielica-Berger, Pawel Rogalski, Anna Pryczynicz, Agnieszka Swidnicka-Siergiejko, Andrzej Dabrowski
Summary: Magnification endoscopy and methylene blue chromoendoscopy can effectively detect intestinal metaplasia (IM). Methylene blue staining is more accurate in indicating IM compared to pit pattern and vessel evaluation. Methylene blue chromoendoscopy can serve as a screening method for detecting advanced OLGIM stages.
CLINICAL ENDOSCOPY
(2023)
Article
Medicine, General & Internal
Justyna Dorf, Konrad Zareba, Anna Pryczynicz, Joanna Matowicka-Karna, Boguslaw Kedra, Piotr Zukowski, Anna Zalewska, Mateusz Maciejczyk
Summary: Gastric cancer is associated with redox imbalance and increased oxidative damage to proteins, lipids, and DNA. Histopathological parameters of the tumor are associated with the level of antioxidants and oxidative damage products. Determination of redox parameters may be useful in the assessment of the tumor progression.
ANNALS OF MEDICINE
(2023)
Article
Immunology
Justyna Dorf, Konrad Zareba, Joanna Matowicka-Karna, Anna Pryczynicz, Katarzyna Guzinska-Ustymowicz, Anna Zalewska, Mateusz Maciejczyk
Summary: The study evaluated the differences in nitrosative stress, protein and DNA oxidation/glycoxidation, as well as pro- and anti-inflammatory cytokines between CRC patients and healthy controls. The results showed that oxidation and glycoxidation products in CRC patients were positively correlated with pro-inflammatory and anti-inflammatory cytokines, indicating that redox damages may promote inflammation in CRC.
JOURNAL OF INFLAMMATION RESEARCH
(2022)