Techniques for quantitative LC–MS/MS analysis of protein therapeutics: advances in enzyme digestion and immunocapture

A rapid approach for characterization of thiol-conjugated antibody–drug conjugates and calculation of drug–antibody ratio by liquid chromatography mass spectrometry

(2015) David Firth et al.   ANALYTICAL BIOCHEMISTRY

Absolute Quantification of the Total and Antidrug Antibody-Bound Concentrations of Recombinant Human α-Glucosidase in Human Plasma Using Protein G Extraction and LC-MS/MS

(2015) Kees J. Bronsema et al.   ANALYTICAL CHEMISTRY

Surfactant-Aided Precipitation/on-Pellet-Digestion (SOD) Procedure Provides Robust and Rapid Sample Preparation for Reproducible, Accurate and Sensitive LC/MS Quantification of Therapeutic Protein in Plasma and Tissues

(2015) Bo An et al.   ANALYTICAL CHEMISTRY

New Mass-Spectrometry-Compatible Degradable Surfactant for Tissue Proteomics

(2015) Ying-Hua Chang et al.   JOURNAL OF PROTEOME RESEARCH

Antibody-Coupled Magnetic Beads Can Be Reused in Immuno-MRM Assays To Reduce Cost and Extend Antibody Supply

(2015) Lei Zhao et al.   JOURNAL OF PROTEOME RESEARCH

An integrated multiplatform bioanalytical strategy for antibody–drug conjugates: a novel case study

(2015) Heather Myler et al.   Bioanalysis

ELISA microplate: a viable immunocapture platform over magnetic beads for immunoaffinity-LC–MS/MS quantitation of protein therapeutics?

(2015) Wenchu Yang et al.   Bioanalysis

Generic Automated Method for Liquid Chromatography–Multiple Reaction Monitoring Mass Spectrometry Based Monoclonal Antibody Quantitation for Preclinical Pharmacokinetic Studies

(2014) Qian Zhang et al.   ANALYTICAL CHEMISTRY

A Novel and Cost Effective Method of Removing Excess Albumin from Plasma/Serum Samples and Its Impacts on LC-MS/MS Bioanalysis of Therapeutic Proteins

(2014) Guowen Liu et al.   ANALYTICAL CHEMISTRY

Multiplexing Determination of Small Cell Lung Cancer Biomarkers and Their Isovariants in Serum by Immunocapture LC-MS/MS

(2014) Silje B. Torsetnes et al.   ANALYTICAL CHEMISTRY

Aspartic Protease Nepenthesin-1 as a Tool for Digestion in Hydrogen/Deuterium Exchange Mass Spectrometry

(2014) Alan Kadek et al.   ANALYTICAL CHEMISTRY

A versatile method for protein-based antigen bioanalysis in non-clinical pharmacokinetics studies of a human monoclonal antibody drug by an immunoaffinity liquid chromatography–tandem mass spectrometry

(2014) Ichio Onami et al.   JOURNAL OF CHROMATOGRAPHY A

LC–MS analysis of polyclonal IgGs using IdeS enzymatic proteolysis for oxidation monitoring

(2014) Yann Leblanc et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

AFFIRM—A Multiplexed Immunoaffinity Platform That Combines Recombinant Antibody Fragments and LC-SRM Analysis

(2014) Anna Säll et al.   JOURNAL OF PROTEOME RESEARCH

High-Affinity Recombinant Antibody Fragments (Fabs) Can Be Applied in Peptide Enrichment Immuno-MRM Assays

(2014) Jeffrey R. Whiteaker et al.   JOURNAL OF PROTEOME RESEARCH

Multiplexed LC–MS/MS Assay for Urine Albumin

(2014) Ashley Beasley-Green et al.   JOURNAL OF PROTEOME RESEARCH

Investigating the Applicability of Antibodies Generated within the Human Protein Atlas as Capture Agents in Immunoenrichment Coupled to Mass Spectrometry

(2014) Tove Boström et al.   JOURNAL OF PROTEOME RESEARCH

Evaluation of the Effect of Trypsin Digestion Buffers on Artificial Deamidation

(2014) Piliang Hao et al.   JOURNAL OF PROTEOME RESEARCH

Influence of the Digestion Technique, Protease, and Missed Cleavage Peptides in Protein Quantitation

(2014) Cristina Chiva et al.   JOURNAL OF PROTEOME RESEARCH

Targeting an acid labile aspartyl–prolyl amide bond as a viable alternative to trypsin digestion to generate a surrogate peptide for LC–MS/MS analysis

(2014) Eliza N Fung et al.   Bioanalysis

High-Sensitivity LC-MS/MS Quantification of Peptides and Proteins in Complex Biological Samples: The Impact of Enzymatic Digestion and Internal Standard Selection on Method Performance

(2013) Kees J. Bronsema et al.   ANALYTICAL CHEMISTRY

Differential Enzyme-Linked Immunosorbent Assay and Ligand-Binding Mass Spectrometry for Analysis of Biotransformation of Protein Therapeutics: Application to Various FGF21 Modalities

(2013) Todd Hager et al.   ANALYTICAL CHEMISTRY

Highly Specific and Sensitive Measurements of Human and Monkey Interleukin 21 Using Sequential Protein and Tryptic Peptide Immunoaffinity LC-MS/MS

(2013) Joe Palandra et al.   ANALYTICAL CHEMISTRY

Immunocapture and LC–MS/MS for selective quantification and differentiation of the isozymes of the biomarker neuron-specific enolase in serum

(2013) Silje Bøen Torsetnes et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Cutting Edge Proteomics: Benchmarking of Six Commercial Trypsins

(2013) Jakob Bunkenborg et al.   JOURNAL OF PROTEOME RESEARCH

PK assays for antibody–drug conjugates: case study with ado-trastuzumab emtansine

(2013) Randall Dere et al.   Bioanalysis

Bioanalytical assay strategies for the development of antibody–drug conjugate biotherapeutics

(2013) Surinder Kaur et al.   Bioanalysis

General LC-MS/MS Method Approach to Quantify Therapeutic Monoclonal Antibodies Using a Common Whole Antibody Internal Standard with Application to Preclinical Studies

(2012) Hongyan Li et al.   ANALYTICAL CHEMISTRY

Quantification of Circulating D-dimer by Peptide Immunoaffinity Enrichment and Tandem Mass Spectrometry

(2012) Weixun Wang et al.   ANALYTICAL CHEMISTRY

Sequential Protein and Peptide Immunoaffinity Capture for Mass Spectrometry-Based Quantification of Total Human β-Nerve Growth Factor

(2012) Hendrik Neubert et al.   ANALYTICAL CHEMISTRY

Differentiation and quantification of endogenous and recombinant-methionyl human leptin in clinical plasma samples by immunocapture/mass spectrometry

(2012) Yan Wang et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Increasing phosphoproteomic coverage through sequential digestion by complementary proteases

(2011) Jason M. Gilmore et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Sensitive Detection of Bacillus anthracis Spores by Immunocapture and Liquid Chromatography–Tandem Mass Spectrometry

(2011) Jérôme Chenau et al.   ANALYTICAL CHEMISTRY

High efficiency and quantitatively reproducible protein digestion by trypsin-immobilized magnetic microspheres

(2011) Liangliang Sun et al.   JOURNAL OF CHROMATOGRAPHY A

Systematic and quantitative comparison of digest efficiency and specificity reveals the impact of trypsin quality on MS-based proteomics

(2011) Julia Maria Burkhart et al.   Journal of Proteomics

The importance of the digest: Proteolysis and absolute quantification in proteomics

(2011) Philip Brownridge et al.   METHODS

Biotherapeutic bioanalysis: a multi-indication case study review

(2011) Heather A Myler et al.   Bioanalysis

Pellet digestion: a simple and efficient sample preparation technique for LC–MS/MS quantification of large therapeutic proteins in plasma

(2011) Zheng Ouyang et al.   Bioanalysis

Immunoaffinity Purification Using Anti-PEG Antibody Followed by Two-Dimensional Liquid Chromatography/Tandem Mass Spectrometry for the Quantification of a PEGylated Therapeutic Peptide in Human Plasma

(2010) Yang Xu et al.   ANALYTICAL CHEMISTRY

Online High-Flow Peptide Immunoaffinity Enrichment and Nanoflow LC-MS/MS: Assay Development for Total Salivary Pepsin/Pepsinogen

(2010) H. Neubert et al.   CLINICAL CHEMISTRY

A novel method for quantitative measurement of a therapeutic monoclonal antibody in the presence of its target protein using enzymatic digestion

(2010) Julie Doucet et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Reproducible microwave-assisted acid hydrolysis of proteins using a household microwave oven and its combination with LC-ESI MS/MS for mapping protein sequences and modifications

(2010) Nan Wang et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

Pressurized Pepsin Digestion in Proteomics

(2010) Daniel López-Ferrer et al.   MOLECULAR & CELLULAR PROTEOMICS

A strategy for liquid chromatography/tandem mass spectrometry based quantitation of pegylated protein drugs in plasma using plasma protein precipitation with water-miscible organic solvents and subsequent trypsin digestion to generate surrogate peptides f

(2010) Steven T. Wu et al.   RAPID COMMUNICATIONS IN MASS SPECTROMETRY

Total on-line analysis of a target protein from plasma by immunoextraction, digestion and liquid chromatography–mass spectrometry

(2009) A. Cingöz et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Magnetic Proteinase K Reactor as a New Tool for Reproducible Limited Protein Digestion

(2008) Marcela Slováková et al.   BIOCONJUGATE CHEMISTRY