Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 961, Issue -, Pages 1-4Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2014.04.053
Keywords
IdeS; Mass spectrometry; IVIG; Oxidation; IgG
Funding
- Catherine Groseil
- Maxime Jube
- Melanie Guilleminault
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Susceptibility of IgGs to oxidation is a significant issue for intravenous immunoglobulin preparations (IVIG) in liquid solution and raises both safety and efficacy concerns. Here we present an optimized chromatography method coupled to mass spectrometry (MS) to determine the oxidation of Fc/2 fragments derived from polyclonal IgGs after IdeS treatment. Separation of the four IgG subclasses was achieved using a diphenyl column and UV/MS detections were used for quantification and characterization. Several oxidized Fc/2 fragments generated by stress conditions were resolved and oxidized methionines were identified. This procedure can be used to monitor the oxidative status of IVIG preparations during formulation or stability studies. (c) 2014. Published by Elsevier B.V.
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