Article
Chemistry, Physical
Jugal Suthar, Alberto Alvarez-Fernandez, Esther Osarfo-Mensah, Stefano Angioletti-Uberti, Gareth R. Williams, Stefan Guldin
Summary: Extracellular vesicles (EVs) are released from various cell types and contain biomolecular content for health and disease identification. However, the heterogeneity of EVs and complexity of biofluids pose challenges to current analytical approaches. This study explores nanostructuring the sensor electrode surface to enhance detection capabilities and achieves significant improvement.
NANOSCALE HORIZONS
(2023)
Article
Chemistry, Physical
Umit Celik, Kuebra Celik, Ihsan Kehribar, Suleyman Celik, Mehmet Z. Baykara
Summary: Forming a fundamental understanding of tribological processes on the atomic scale has the potential to revolutionize the control of friction and wear in macroscopic mechanical systems. However, current methods for studying tribology at the nanoscale, such as atomic force microscopy, are limited in scanning speed compared to macroscopic mechanical processes. In this study, a new method combining atomic force microscopy with quartz crystal microbalance measurements is proposed for high-speed nanoscale tribology.
APPLIED SURFACE SCIENCE
(2023)
Article
Materials Science, Multidisciplinary
Diethelm Johannsmann, Judith Petri, Christian Leppin, Arne Langhoff, Hozan Ibrahim
Summary: Fouling at a hot reactor wall during emulsion polymerization was studied in situ using QCM-D. Transient maxima in resonance bandwidth were observed, which cannot be explained by film resonance theory. The presence of particulate nature in the sample explains the observed phenomena. Simulation based on FD-LBM supports this argument and demonstrates the capabilities of FD-LBM.
RESULTS IN PHYSICS
(2023)
Article
Food Science & Technology
Qiang-Wei Zhan, Yan Huang
Summary: Flash nano-precipitation (FNP) efficiently produces phospholipids stabilized nanoparticles with more uniform coverage and different stability depending on the core content. The study reveals that particle size and stability can be precisely predicted using specific models and theories.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Chemistry, Applied
Hiroaki Kaga, Aoi Nakamura, Masanori Orita, Koji Endo, Masaaki Akamatsu, Kenichi Sakai, Hideki Sakai
Summary: In this study, the removal performance of sophorolipids on a beta-glucan film, a model biofilm, was evaluated using QCM-D measurements. The results showed that the removal performance increased with the concentration of sophorolipids, and two processes were involved in the removal mechanism: water adsorption and film removal from the sensor surface. It was suggested that sophorolipids interfere with the hydration of the beta-glucan film and suppress increases in its viscosity, which is expected to be an essential factor for the removal of the beta-glucan film. Sophorolipids demonstrate potential as eco-friendly agents for biofilm removal in cosmetics.
JOURNAL OF OLEO SCIENCE
(2022)
Article
Biophysics
Daniel Pereiraa, Andreas Santamaria, Nisha Pawar, Javier Carrascosa-Tejedor, Mariana Sardo, Luis Mafra, Eduardo Guzman, David J. Owen, Nathan R. Zaccai, Armando Maestro, Ildefonso Marin-Montesinos
Summary: The combination of solid-supported lipid bilayer (SLB) models and surface sensitive techniques provides quantitative information about molecular interactions and lipid distributions. This study designed complex SLBs to mimic cellular plasma membranes, and incorporated phosphatidylinositol 4,5-bisphosphate (PtdIns4,5P2) lipids and synthetic lipo-peptides. Results showed that the adsorption and fusion kinetics of PtdIns4,5P2 were influenced by Mg2+ and higher concentrations of PtdIns4,5P2 led to more homogeneous SLBs. The presence of PtdIns4,5P2 clusters was observed using AFM, while NR provided insights into the structural organization of the SLBs and the role of CD4-derived cargo peptides.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Review
Chemistry, Analytical
Kaimin Wu, Feizhi Kong, Jingjing Zhang, Ying Tang, Yao Chen, Long Chao, Libo Nie, Zhao Huang
Summary: Single-nucleotide polymorphisms (SNPs) are the most common form of genetic variation in the human genome, and are closely related to individual differences. SNP detection with simple preparation and operational procedures, high affinity and specificity, and cost-effectiveness has been a challenge. This review provides an overview on improvements for SNP biosensing based on fluorescent and electrochemical methods, and presents the advantages, disadvantages, and challenges of each method.
Article
Nanoscience & Nanotechnology
Nils Luedecke, Marek Bekir, Stephan Eickelmann, Matthias Hartlieb, Helmut Schlaad
Summary: Inspired by mussel proteins, a platform for protein-repellent macromolecules based on poly(2-ethyl-2-oxazoline) carrying catechol and cationic functional groups was developed. The polymers with catechol units showed a strong tendency to form surface-bound layers on different substrates, while the ones with additional cationic units were able to form defined and durable polymer films. These coatings prevented the attachment of model proteins, offering a biomimetic approach to nonfouling surface coatings.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Engineering, Electrical & Electronic
Ilia Chiniforooshan Esfahani, Hongwei Sun
Summary: The micropillar-enhanced QCM viscosity measurement device developed in this work achieves ultra-sensitive viscosity measurement of a sample droplet by utilizing the coupled vibration between micropillars and quartz substrate.
SENSORS AND ACTUATORS A-PHYSICAL
(2023)
Article
Chemistry, Analytical
Philipp Ortner, Maren Umlandt, Nino Lomadze, Svetlana Santer, Marek Bekir
Summary: The quartz crystal microbalance with dissipation (QCM-D) has been widely used for investigating the external stimuli responsiveness at solid-liquid interfaces. However, in light responsive adsorption investigation, the quartz crystal itself reacts to optical stimulation, which poses challenges in data interpretation. This article introduces a simple guideline for correcting the measurement artifacts and minimizing the impact of light induced detuning (LID).
ANALYTICAL CHEMISTRY
(2023)
Article
Cell Biology
Eleni Priglinger, Juergen Strasser, Boris Buchroithner, Florian Weber, Susanne Wolbank, Daniela Auer, Eva Grasmann, Claudia Arzt, Dmitry Sivun, Johannes Grillari, Jaroslaw Jacak, Johannes Preiner, Mario Gimona
Summary: Interest in using mesenchymal stem cell derived extracellular vesicles (MSC-EVs) as therapeutic agents has grown in the past decade. This study established and verified quartz crystal microbalance (QCM) as a highly sensitive label-free immunosensing technique for characterizing clinically approved umbilical cord MSC-EVs. By using QCM along with common characterization methods, specific detection of EVs via EV and MSC markers was achieved, allowing for quantitative determination of the ratio of marker-specific EVs versus non-vesicular particles.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Chemistry, Physical
Jianlong Ye, Yufeng Zhang, Jianqiang Meng
Summary: Hydrophobic charge-induction chromatography (HCIC) is a novel technology used for antibody purification, and the interactions between IgG and HCIC ligands have been studied. The study found that the Au-MBI sensor exhibited excellent performance in IgG adsorption, possibly leading to changes in the adsorbed IgG layer structure due to alterations in protein conformation. The HCIC technology shows promising potential for accurate theoretical guidance in protein adsorption and antibody purification procedures.
APPLIED SURFACE SCIENCE
(2022)
Article
Environmental Sciences
Changyong Wu, Liya Fu, Yue Wang, Chunli Wan
Summary: This study investigated the adsorption characteristics of marine dissolved organic matters (MDOMs) on the solid-liquid interface in coastal waters. It found that the organic macromolecules in MDOMs have viscoelastic properties and the adsorption process is dynamic, with MDOMs concentration being an important driving force. The three macromolecules in MDOMs, including acidic polysaccharides, protein-like, and polycarboxylate-type humic acids, exhibit different adsorption abilities and characteristics at the solid-liquid interface, with acidic polysaccharides showing sustained adsorption ability.
Article
Chemistry, Physical
Wael A. Amer, Ahmed F. Rehab, Mona E. Abdelghafar, Nagy L. Torad, Ahmed S. Atlam, Mohamad M. Ayad
Summary: Carbon quantum dots (CQDs) were synthesized using a green and economical hydrothermal method, and their optical and structural properties were studied. The sensing properties of CQDs, particularly towards volatile formaldehyde, were investigated, showing high sensitivity and potential for formaldehyde vapor detection.
Article
Chemistry, Multidisciplinary
Ester Canepa, Davide Bochicchio, Giorgia Brosio, Paulo Henrique Jacob Silva, Francesco Stellacci, Silvia Dante, Giulia Rossi, Annalisa Relini
Summary: Membrane fusion is essential for eukaryotic cell functionality and is regulated by specialized proteins, lipid composition, and ion environment. Liposomes decorated with amphiphilic Au nanoparticles (AuLips) have been shown to act as tunable fusion machinery, triggered by divalent ions and tunable by liposome cholesterol content. This research provides new mechanistic details on the fusogenic activity of AuNPs and demonstrates their potential as artificial fusogenic agents for biomedical applications.
Article
Engineering, Environmental
Miaolu He, Lei Wang, Yongtao Lv, Xudong Wang, Jiani Zhu, Yan Zhang, Tingting Liu
CHEMICAL ENGINEERING JOURNAL
(2020)
Article
Multidisciplinary Sciences
Jin Wang, Zhijie Zhang, Jiani Zhu, Mengtao Tian, Shuchang Zheng, Fudi Wang, Xudong Wang, Lei Wang
NATURE COMMUNICATIONS
(2020)
Article
Engineering, Chemical
Rui Miao, Youya Zhou, Pei Wang, Wei Lu, Pu Li, Xiaoyan Li, Lei Wang
Summary: The research discovered that pre-chlorination and pre-ozonation have different effects on membrane fouling behavior, with variations in the interaction forces between BSA molecules at the membrane surface. Chlorination breaks the disulfide bonds between BSA molecules, while ozonation promotes their formation, resulting in different protein deposition behaviors on the membrane surface and corresponding fouling layer structures.
JOURNAL OF MEMBRANE SCIENCE
(2021)
Article
Engineering, Chemical
Feixiang Gong, Zhitao Fan, Wenke Xia, Meng Zhang, Lei Wang, Xudong Wang, Xinbing Chen
Summary: By adjusting the chemical structures of functional groups, the properties of CardoPES-C-2-R(Cl-) membranes can be readily adjusted, and these membranes exhibit ultrahigh selectivity and stability in DD.
JOURNAL OF MEMBRANE SCIENCE
(2021)
Article
Engineering, Environmental
Xuerui Bai, Maiqian Nie, Zhenjun Diwu, Lei Wang, Hongyun Nie, Yan Wang, Qiuyue Yin, Bo Zhang
Summary: This study successfully produced high purity 2-HMS product by extracting 2-HMS from high concentration phenolic wastes. Under optimized conditions, a high conversion rate and good product quality were achieved.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Engineering, Chemical
Xiaorong Meng, Jing Ren, Jiazhi Chen, Jinfeng Song, Huiqi Zheng, Lei Wang, DanXi Huang
Summary: Ag3PO4 and AgBr-Ag3PO4 were deposited on the surface of nanofiltration membrane with tannic acid (TA) and toluene-2,4 diisocyanate (TDI) as functional layers to obtain composite NF membranes (FTA and FTAB) with visible catalytic activity. FTA and FTAB showed good photochemical properties, stable degradation rates for pollutants like SMZ, and anti-pollution self-cleaning performances.
JOURNAL OF MEMBRANE SCIENCE
(2021)
Article
Engineering, Chemical
Rui Miao, Bingpeng Ma, Pu Li, Pei Wang, Lei Wang, Xiao-yan Li
Summary: Preozonation of casein solutions in dairy wastewater can effectively mitigate membrane fouling in ultrafiltration. The process disrupts interactions between casein molecules, leading to the formation of a more open-and-loose fouling layer on the membrane, ultimately reducing fouling levels.
JOURNAL OF MEMBRANE SCIENCE
(2021)
Article
Engineering, Chemical
Xiaochen Zhao, Xudong Wang, Yonghao Dong, Hongmin Zhang, Wei Zhao, Jin Wang, Lei Wang
Summary: By intercalating and introducing adhesive molecules, the interlayer spacing of graphitic carbon nitride nanosheets is expanded, resulting in larger interlayer channels and improved separation performance and water flux of the composite membrane.
JOURNAL OF MEMBRANE SCIENCE
(2022)
Review
Chemistry, Multidisciplinary
Jin Wang, Huijiao Zhou, Shangzhen Li, Lei Wang
Summary: This review discusses the construction of nanosheet-based membranes and their effects on ion transport behavior. The applications of these membranes in desalination, ion recovery, and energy conversion are also presented. Finally, the challenges and future research directions in this field are discussed.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Engineering, Chemical
Jin Wang, Sensen Liu, Shuchang Zheng, Shangzhen Li, Di Wang, Miaolu He, Lei Wang, Xudong Wang
Summary: The exceptional ion release and storage ability of biological ion channels have attracted significant attention for various life activities. This study presented the ion transport behavior in a vertically-oriented three-sectional Ti3C2Tx membrane with asymmetric charge distribution. By grafting silane molecules onto the surface, the surface charge polarity of the Ti3C2Tx nanosheet was changed, leading to a diode-like ion transport property in the heterogenous membrane. Numerical simulations based on PNP equations showed that the directional ion diffusion was attributed to ion accumulation and depletion in the nanochannels under different potential biases. The heterogenous membrane also showed potential in desalination and ion storage applications.
JOURNAL OF MEMBRANE SCIENCE
(2023)
Article
Engineering, Chemical
Miaolu He, Ze Liu, Lei Wang, Jiani Zhu, Jin Wang, Rui Miao, Yongtao Lv, Xudong Wang
Summary: In this study, a modified Ti3C2Tx membrane (C@TM) was prepared by mixing Ti3C2Tx with CMC and cross-linking the CMC, which effectively reduced the swelling of the membrane and improved its mechanical strength. The C@TM exhibited efficient ion sieving performance and excellent long-term stability during operation.
JOURNAL OF MEMBRANE SCIENCE
(2023)
Review
Chemistry, Multidisciplinary
Shangzhen Li, Jin Wang, Yongtao Lv, Zheng Cui, Lei Wang
Summary: This review provides a comprehensive summary of the key factors, including the physicochemical properties of nanomaterials, membrane structural properties, and external environmental factors, that significantly impact the conversion performance of osmotic energy through nanochannel membranes. The aim is to offer crucial guidance for the design of high-performance nanochannel membranes.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Engineering, Chemical
Miao Rui, Yupeng Wang, Haoxue Ran, Yanfei Li, Chengshu Yang, Lei Wang
Summary: The influencing mechanism of pre-ozonation on membrane fouling by secondary effluent organic matter (EfOM) was analyzed based on drag force, adhesion forces, and the properties of EfOM and membranes. It was found that pre-ozonation could either exacerbate or mitigate EfOM fouling, depending on the relative magnitudes of the drag force and the adhesion forces. For membranes with a low molecular weight cutoff, pre-ozonation exacerbated fouling due to increased adhesion forces. However, a higher molecular weight cutoff led to increased drag force, reducing fouling but increasing irreversible fouling. Membrane structure-related steric effects could weaken the influence of drag force on fouling.
JOURNAL OF MEMBRANE SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Jin Wang, Di Wang, Zeyuan Song, Na Jiang, Shangzhen Li, Yufei Zhang, Bo Huang, Huijiao Zhou, Zheng Cui, Lei Wang
Summary: This research demonstrates light-controlled ion transport in a two-dimensional Ti3C2Tx/g-C3N4 heterogenous nanochannel, driven by a photo-electric/thermal synergistic effect. Under illumination, the separation of photoexcited charge carriers prompts spontaneous ion migration, further reinforced by the light-to-heat conversion of Ti3C2Tx channels. Effective modulation of ionic diffusion is achieved even under natural sunlight, enabling efficient harvesting of osmotic power and successful conversion of ionic energy into electricity. This study opens up new possibilities for the design of intelligent light-triggered nanochannels for solar energy conversion and storage.
ENERGY & ENVIRONMENTAL SCIENCE
(2023)
Review
Engineering, Environmental
Jiajin Hao, Lei Wang, Xudong Wang, Jin Wang, Miaolu He, Xinyue Zhang, Jiaqi Wang, LuJie Nie, JingXian Li
Summary: This study provides a detailed overview of the preparation and doping of polyaniline conductive membranes, evaluating their effects on membrane properties. Additionally, it summarizes the research on their applications in water treatment and antifouling.
ENVIRONMENTAL SCIENCE-WATER RESEARCH & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Rong-Gui Zhu, Chang-Gui Pan, Feng-Jiao Peng, Chao-Yang Zhou, Jun-Jie Hu, Kefu Yu
Summary: This comprehensive survey investigated the occurrence, bioaccumulation, and trophic magnification of parabens and their metabolite 4-HB in a marine food web. Results showed that parabens were the predominant pollutants in marine organisms, with significant bioaccumulation from sediments. The estimated trophic magnification factor indicated biomagnification for MeP and trophic dilution for 4-HB. Overall, the risks for humans consuming marine organisms were found to be low.
Article
Engineering, Environmental
Andres F. Torres-Franco, Deborah Leroy-Freitas, Cristina Martinez-Fraile, Elisa Rodriguez, Pedro A. Garcia-Encina, Raul Munoz
Summary: Anaerobic and microalgae-based technologies have emerged as sustainable alternatives for municipal wastewater treatment. However, the presence of viruses in the treated wastewater is a major concern for reuse applications. This study assessed the ability of these technologies to reduce viruses during secondary wastewater treatment. The results showed that all technologies were effective in reducing the concentration of viruses, with microalgae-based treatment exhibiting the highest potential for reducing the disinfection requirements of treated wastewater.
Article
Engineering, Environmental
Young Gwang Kim, Sae Yun Kwon, Spencer J. Washburn, Scott C. Brooks, Ji Won Yoon, Lucien Besnard
Summary: The study uses Hg isotope ratios to identify the sources and exposure pathways of mercury in bivalves, finding that dissolved Hg phases in the water column are the primary source and exposure pathway to bivalves. This provides new insights into using bivalves as bioindicators for sediment quality monitoring.
Article
Engineering, Environmental
Hui Geng, Ying Xu, Rui Liu, Dianhai Yang, Xiaohu Dai
Summary: This study investigates the effect of cation exchange resin (CER) on the sequential recovery of hydrogen and methane from anaerobic digestion (AD) and the corresponding mechanisms. The results show that CER can simultaneously enhance the production of hydrogen and methane by promoting the solubilisation, hydrolysis, and acidification of organic matter. Additionally, CER facilitates effective contact between bacteria and organic particulates and reduces the energy barrier for mass transfer during methane production. The study also reveals changes in the microbial community structure and metagenomics during the AD process.
Article
Engineering, Environmental
Xiaojing Lin, Zhan Jin, Shunfeng Jiang, Zhiquan Wang, Suqing Wu, Ke Bei, Min Zhao, Xiangyong Zheng
Summary: Dehumidification combined with addition of absorbent resin supplement (ARS) was used to achieve rapid evaporation of non-pretreated urine, resulting in high water evaporation efficiency and nutrient recovery.
Article
Engineering, Environmental
Yangli Che, Chaoran Lin, Shen Li, Jiao Liu, Longhai Zhu, Shilei Yu, Nan Wang, Haoshuai Li, Mutai Bao, Yang Zhou, Tonghao Si, Rui Bao
Summary: Hydrodynamic processes play a crucial role in the transmission of sediments, microbial assembly, and organic carbon redistribution in the ocean. Through experiments and analysis, we found that hydrodynamics shape the assembly of microbial communities and control the redistribution of different sourced organic carbon, thereby influencing microbial-mediated biogeochemical transformation.
Article
Engineering, Environmental
Chao Chen, Yu Yang, Nigel J. D. Graham, Zhenyu Li, Xingtao Yang, Zhining Wang, Nadia Farhat, Johannes S. Vrouwenvelder, Li -an Hou
Summary: The fouling of seawater reverse osmosis membranes is a persistent challenge in desalination. This study monitored the operational performance of a desalination plant for 7 years and the fouling development in different areas of membrane modules. The findings showed that operational performance declined over time and fouling mainly occurred at the feed side of the modules, with the highest microbial diversity. Keystone species like Chloroflexi and Planctomycetes played an important role in maintaining community structure and biofilm maturation. Polysaccharides, soluble microbial products, marine humic acid-like substances, and inorganic substances contributed to fouling. Overall, biofouling had a significant impact on membrane fouling after 7 years of operation.
Article
Engineering, Environmental
Dan Li, Jieyi Sun, Yibo Fu, Wentao Hong, Heli Wang, Qian Yang, Junhong Wu, Sen Yang, Jianhui Xu, Yunfei Zhang, Yirong Deng, Yin Zhong, Ping'an Peng
Summary: Sulfidation-oxidation treatment of magnetite (Fe3O4) enhances the production of dark center dot OH, which can efficiently degrade dissolved organic matter (DOM) and accelerate carbon cycling.
Article
Engineering, Environmental
Cheng Yu, Kaijun Wang, Kaiyuan Zhang, Ruiyang Liu, Pingping Zheng
Summary: This study implemented a microaerobic-aerobic configuration in a full-scale municipal wastewater treatment facility and investigated the effects on sludge characteristics, pollutant removal, microbial community, and granulation mechanisms. The results showed successful transition from flocculent-activated sludge to well-defined AGS after two months of operation. The primary pathways for pollutant removal were simultaneous nitrification, denitrification, and phosphorus removal. Moreover, the incorporation of internal separators induced shifts in the flow pattern, which promoted granulation.
Article
Engineering, Environmental
Zhe Zhang, Shaoyang Hu, Guangrong Sun, Wei Wang
Summary: Halogenated aromatic disinfection byproducts (DBPs), such as halogenated phenols, have garnered widespread attention due to their high toxicity and prevalence. This study reports on the analysis, occurrence, and cytotoxicity of a group of emerging halogenated aromatic DBPs, known as halogenated polyhydroxyphenols (HPPs), in drinking water.
Article
Engineering, Environmental
Shengyue Chen, Jinliang Huang, Peng Wang, Xi Tang, Zhenyu Zhang
Summary: Accurate prediction of river water quality is crucial for sustainable water management. This study introduces wavelet analysis and transfer learning techniques to assist LSTM modeling, proposing a newly coupled modeling approach that improves short-term prediction of river water quality.
Article
Engineering, Environmental
Bang Du, Xinmin Zhan, Piet N. L. Lens, Yifeng Zhang, Guangxue Wu
Summary: Efficient anaerobic digestion relies on the cooperation of different microorganisms with different metabolic pathways. This study investigated the effects of different operational modes and the addition of powdered activated carbon (PAC) on ethanol metabolic pathways. The results showed that the SBR mode and the presence of CO2 facilitated ethanol metabolism towards propionate production, while the CFR mode with extended solids retention time enriched Geobacter. Adjusting operational modes and PAC addition can modulate anaerobic ethanol metabolism and enrich Geobacter.
Article
Engineering, Environmental
Wanfa Wang, Si-Liang Li, Jun Zhong, Yuanbi Yi, Fujun Yue, Zenglei Han, Qixin Wu, Ding He, Cong-Qiang Liu
Summary: This study compares the carbon biogeochemical processes in karst and non-karst regions within large thermal stratified river-reservoir systems. The results demonstrate that karst reservoirs have a reduced potential for carbon emissions and highlight the importance of considering geologic settings to improve accuracy in regional and global CO2 emission estimates.
Article
Engineering, Environmental
Chunxia Jiang, Zelong Zhao, Dong Zhu, Xiong Pan, Yuyi Yang
Summary: This study analyzed the occurrence and distribution of antibiotic resistance genes (ARGs) in different environmental media of the Yangtze River using metagenomics. Core resistome dominated by multidrug resistance genes was found in all samples, while rare resistome dominated by various resistance genes was more prevalent in plasmids. Specific bacteria were identified as hosts for both core and rare resistomes, with high clinical concern ARGs found in the rare resistome. Particle-associated environment provided the most ideal conditions for resistome hosts. This study provided insights into the genetic locations of ARGs and the community assembly mechanisms of ARG hosts in freshwater environments.
Article
Engineering, Environmental
Yu Zhang, Yongtao He, Linchun Jia, Lei Xu, Zheng Wang, Yueling He, Ling Xiong, Xumeng Lin, Hong Chen, Gang Xue
Summary: By synergizing organic carbon source, thiosulfate, and zero-valent iron, efficient mixotrophic denitrification of oligotrophic secondary effluent can be achieved. Thiosulfate plays a vital role in promoting TN removal efficiency, while corrosion of Fe0 releases OH- to neutralize H+ from thiosulfate-driven denitrification, creating a suitable environment for denitrification. The coordination of thiosulfate and Fe0 maintains the dominance of Thiobacillus for denitrification.