Review
Biotechnology & Applied Microbiology
Bhagyashree Bachhav, Jacopo de Rossi, Carlos D. Llanos, Laura Segatori
Summary: The production of high-quality recombinant proteins is crucial for the continuous supply of biopharmaceuticals, such as therapeutic antibodies. Engineering mammalian cell factories faces limitations due to proteotoxic stress caused by abnormal accumulation of protein folding intermediates, leading to apoptosis. This review discusses advances in cell engineering, focusing on controlling the expression of recombinant proteins at various levels and highlights the opportunities to use synthetic biology tools for designing programmable cell factories for improved biomanufacturing of therapeutic proteins.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Biochemical Research Methods
Yiting Shi, Tianyu Dong, Boxuan Zeng, Mingdong Yao, Ying Wang, Zexiong Xie, Wenhai Xiao, Yingjin Yuan
Summary: This study successfully manipulated the production of parthenolide, a sesquiterpene lactone and precursor for the anti-glioblastoma drug ACT001, in microorganisms through a comprehensive strategy involving P450 source screening, NADPH supply, and endoplasmic reticulum size rewiring. The highest titers of parthenolide and costunolide achieved in microbes were 31.0 mg/L and 648.5 mg/L, respectively, demonstrating the potential for sustainable production of sesquiterpene lactones and highlighting a general strategy for manipulating multiple plant-derived P450s in microbes.
ACS SYNTHETIC BIOLOGY
(2022)
Review
Biology
Chaofeng Li, Jiyang Zheng, Yushuang Wu, Xiaotong Wang, Hui Shao, Dong Yan
Summary: Light-driven synthetic biology utilizes cyanobacteria to convert light energy into chemicals, contributing to carbon neutrality and green production. This technology has made significant progress in fuel and chemical synthesis, but still faces challenges such as selecting suitable chassis cells, metabolic engineering strategies, and methods for utilizing high-value CO2.
Review
Biotechnology & Applied Microbiology
Feng Li, Meng Meng Chen, Hui Min Zhang, Qing Ping Wu, Yun Bin Han
Summary: Ginsenoside compound K (CK) is a major metabolite of ginsenosides that can be absorbed in the body. Traditional methods for producing CK have limitations in terms of production and cost. However, recent advancements in microbial cell factories have enabled the de novo production of CK using synthetic biology-based strategies. By optimizing metabolic engineering strategies, the yield of CK has significantly increased, making this approach commercially feasible. This review summarizes the latest developments in the production of CK using microbial cell factories.
BIOTECHNOLOGY LETTERS
(2023)
Review
Biotechnology & Applied Microbiology
Naghmeh Poorinmohammad, Eduard J. Kerkhoven
Summary: Yarrowia lipolytica is a promising yeast for various biotechnological applications, with metabolic engineering and systems biotechnology enhancing its potential for rational strain design in cell factories. The advancement in molecular and genetic tools have allowed for in-depth understanding of cellular mechanisms, enabling the development of favorable production metrics.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biochemical Research Methods
Jin-Tao Cheng, Jia-Hui Yu, Chen-Fan Sun, Fei Cao, You-Min Ying, Zha-Jun Zhan, Wen-Ju Li, Xin-Ai Chen, Qing-Wei Zhao, Yong-Quan Li, Li-She Gan, Xu-Ming Mao
Summary: Fungal natural products are valuable sources of clinical drugs, but many potential biosynthetic gene clusters remain silent in the laboratory. Researchers have successfully activated endogenous gene clusters in fungi like Calcarisporium arbuscula and identified an efficient expression system, offering a new approach for mining bioactive natural products from fungi.
ACS SYNTHETIC BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Dongsoo Yang, Hyunmin Eun, Cindy Pricilia Surya Prabowo
Summary: Polyketides, a type of natural products, have versatile applications such as pharmaceuticals, nutraceuticals, and cosmetics. Aromatic polyketides, including type II and III polyketides, are important chemicals for human health, such as antibiotics and anticancer agents. However, they are mainly produced from challenging sources like soil bacteria or plants. To address this issue, metabolic engineering and synthetic biology have been used to efficiently engineer model microorganisms for enhanced production of aromatic polyketides. This review discusses the recent advancements and future prospects of metabolic engineering and synthetic biology strategies in the production of type II and type III polyketides in model microorganisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biotechnology & Applied Microbiology
H. Pramastya, Y. Song, E. Y. Elfahmi, S. Sukrasno, W. J. Quax
Summary: Bacillus subtilis is being developed into terpenoids cell factories due to its safe status and high isoprene precursor biosynthesis capacity. This review provides insights into the MEP pathway enzymes, isomerization of C5 isoprene precursors, and steps to improve terpenoids production in B. subtilis. The study also discusses genetic tools and strategies for further development of terpenoid cell factories in B. subtilis.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Review
Plant Sciences
Alice Stra, Lamyaa O. Almarwaey, Yagiz Alagoz, Juan C. Moreno, Salim Al-Babili
Summary: Carotenoids are natural pigments produced by plants, algae, bacteria, and microorganisms, playing essential roles in light-harvesting and protecting the photosynthetic apparatus. They are also precursors of important metabolites like vitamin A and phytohormones. Genetic engineering and metabolic engineering have been used to enhance carotenoid content and production, with recent advances including CRISPR technologies and viral vectors. Manipulating carotenoid biosynthesis can improve crop yield and growth, making it an important target for crop improvements. This article provides an overview of carotenoid biosynthesis and discusses the latest advances in synthetic carotenoid metabolism in plants and microorganisms.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Biotechnology & Applied Microbiology
Varsha K. Singh, Sapana Jha, Palak Rana, Amit Gupta, Ashish P. Singh, Neha Kumari, Sonal Mishra, Prashant R. Singh, Jyoti Jaiswal, Rajeshwar P. Sinha
Summary: Currently, the synthetic UV-absorbing compounds used in sunscreens are derived from non-renewable petrochemicals. To address this issue, it is necessary to develop a sustainable manufacturing process for the production of naturally occurring UV-absorbing compounds called mycosporine-like amino acids (MAAs). However, the commercial production of MAAs for sunscreens is currently not feasible due to low yield and the lack of fermentation technology. This review aims to provide insights into the biochemical properties of MAAs, biosynthetic gene clusters, carbon flux processes, and strategies for engineering suitable cyanobacteria for cost-effective production of natural sunscreens.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Yuan Zhu, Jianxiu Li, Longyun Peng, Lijun Meng, Mengxue Diao, Shuiyuan Jiang, Jianbin Li, Nengzhong Xie
Summary: In this study, a highly efficient yeast cell factory was constructed for the production of high-value-added PPD and its derivatives. By modulating gene expression, introducing dynamic control, and utilizing inexpensive substrates, large-scale PPD production was achieved, setting new records.
MICROBIAL CELL FACTORIES
(2022)
Article
Multidisciplinary Sciences
Enrico Orsi, Nico J. Claassens, Pablo Nikel, Steffen N. Lindner
Summary: Synthetic biology has revolutionized the ability to redesign microbial metabolic networks by combining metabolic pathway-modularization with growth-coupled selection schemes. This powerful tool enables deep rewiring of cell factories' biochemistry for rational bioproduction.
NATURE COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Mengting Deng, Xueqin Lv, Long Liu, Jianghua Li, Guocheng Du, Jian Chen, Yanfeng Liu
Summary: The increasing global population and protein demand pose challenges for food supply. Synthetic biology-based microbial cell factories are promising for scalable and cost-effective production of alternative proteins, especially milk proteins.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biotechnology & Applied Microbiology
Pingping Wang, Jiali Wang, Guoping Zhao, Xing Yan, Zhihua Zhou
Summary: Through engineering yeast, the research team successfully achieved high-titer production of CK from glucose, resulting in the highest reported production of CK in microbes. This breakthrough enables a stable supply of CK for further study and medical applications.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2021)
Article
Environmental Sciences
Valentin Romanovski, Xintai Su, Lijuan Zhang, Andrei Paspelau, Andrey Smorokov, Ali Akbari Sehat, Abayomi Adewale Akinwande, Natalia Korob, Maksim Kamarou
Summary: A green approach was developed for waste recycling and industrial wastewater treatment using the filtrate of synthetic gypsum production. By synthesizing magnetic sorbents and photocatalysts from the iron sulfate in the concentrated filtrate, efficient oil sorption and photodegradation of dye were achieved. The filtrate containing sulfur, calcium, magnesium, and sodium also showed potential as a complex fertilizer.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Wen-Fang Wang, Han Xiao, Jian-Jiang Zhong
BIOTECHNOLOGY AND BIOENGINEERING
(2018)
Article
Biotechnology & Applied Microbiology
Zhi-Qiang Du, Jian-Jiang Zhong
BIOTECHNOLOGY AND BIOENGINEERING
(2018)
Review
Biochemical Research Methods
Yang-Chun Yong, Yan-Zhai Wang, Jian-Jiang Zhong
CURRENT OPINION IN BIOTECHNOLOGY
(2018)
Correction
Biotechnology & Applied Microbiology
Zhi-Qiang Du, Yuan Zhang, Zhi-Gang Qian, Han Xiao, Jian-Jiang Zhong
BIOTECHNOLOGY AND BIOENGINEERING
(2018)
Article
Oncology
Yi Luo, Ya-Wei Cheng, Chang-Yin Yu, Ru-Ming Liu, Yu-Jie Zhao, Dai-Xiong Chen, Jian-Jiang Zhong, Jian-Hui Xiao
EXPERIMENTAL CELL RESEARCH
(2019)
Article
Biotechnology & Applied Microbiology
Ping-An Wang, Han Xiao, Jian-Jiang Zhong
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Zheng-Yan Lu, Jian-Jiang Zhong
PROCESS BIOCHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Han Li, Jian-Jiang Zhong
PROCESS BIOCHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Siqin Cai, Han Xiao, Xiaozheng Wang, Shuangjun Lin, Jian-Jiang Zhong
PROCESS BIOCHEMISTRY
(2020)
Review
Engineering, Chemical
Ting-Ting Liu, Han Xiao, Jian-Hui Xiao, Jian-Jiang Zhong
Summary: Terpenoids are high value-added natural products with various biological functions, and genetically engineered microorganisms have advantages in their production. Oxygen is crucial for microbial metabolism and growth, and proper oxygen supply is essential for efficient terpenoid production by engineered microorganisms.
CHINESE JOURNAL OF CHEMICAL ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Ting-Ting Liu, Jian-Jiang Zhong
Summary: The study demonstrated that oxygen supply has a positive effect on DHLDOA production by yeast fermentation, leading to a significant increase in DHLDOA titer. Under specific initial KLa conditions, DHLDOA production is influenced by pyruvate accumulation, citrate levels, and the activities of CYP5150L8 and CYP5139G1.
PROCESS BIOCHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Wen-Fang Wang, Han Xiao, Jian-Jiang Zhong
Summary: In this study, a new GA, DHLDOA, was successfully synthesized in engineered yeast by oxidation of HLDOA using CYP5139G1 from Ganoderma lucidum. Enzymatic experiments confirmed the activity of CYP5139G1, and strategies to increase DHLDOA production were also explored.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jing Zhang, Ling-Xiao Gao, Wei Chen, Jian-Jiang Zhong, Chao Qian, Wen-Wen Zhou
Summary: Doxorubicin, a widely used antitumor drug, is currently produced through a chemical conversion method, which is costly and environmentally polluting. In this study, we expressed and optimized the key enzyme, cytochrome daunorubicin C-14 hydroxylase, for doxorubicin production. Through rational design, we obtained a mutant enzyme, DoxA(P88Y), which showed a 56% increase in bioconversion efficiency compared to the wild-type enzyme. Molecular dynamics simulation revealed the enhanced binding stability between the mutant enzyme and the substrate, daunorubicin, contributing to the improved catalytic activity. This work provides a foundation for further development of doxorubicin and facilitates its industrial production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Xihong Liang, Wenya Hu, Jian-Jiang Zhong
Summary: This study compared the properties of bacterial cellulose (BC) produced by two different strains and found that BC from the mutated strain CGMCC 21569 had higher molecular weight, crystallinity, fiber diameter, and thermal stability. The composite film prepared from this strain showed higher Young's modulus without a reduction in tensile strength and elongation at break. Comparative genome analysis suggested that genes bcsA_1, acsAB_1, epsJ_1, and bglB_1 may affect the biosynthesis of BC, specifically its chain length. This study provides interesting information for genetically modifying strains to produce customized BC for manufacturing excellent BC composite films.
PROCESS BIOCHEMISTRY
(2023)
Article
Chemistry, Physical
Yuhuan Luo, Zeqian Du, Chenjian Jiang, Ziqian Yu, Jian-Jiang Zhong, Ting Shi, Han Xiao
Summary: A new type of CYP512W2 cytochrome P450 enzyme was discovered in this study, which can efficiently catalyze the production of important GAs. Further engineering can increase the yield and reveal the catalytic mechanism.
