Article
Engineering, Biomedical
Jiabin Zhang, Ming Zhang, Rongcai Lin, Yuguang Du, Liming Wang, Qingqiang Yao, Andrew Zannettino, Hu Zhang
Summary: Stem cell therapy using magnetic scaffolds has been developed to treat osteoarthritis. The magnetic scaffolds enhance chondrogenesis of mesenchymal stem cells through mechanical stimulation and magnetic field effects. The fiber topography of the scaffolds can be tuned with different coatings on magnetic nanoparticles, which selectively up-regulate chondrogenesis or osteogenesis-related genes.
Article
Cell & Tissue Engineering
Maiko Sezaki, Subinoy Biswas, Sayuri Nakata, Motohiko Oshima, Shuhei Koide, Nicole Pui Yu Ho, Nobukazu Okamoto, Takeshi Miyamoto, Atsushi Iwama, Hitoshi Takizawa
Summary: This study characterizes the heterogeneity of hMSCs and their ability to efficiently form humanized ossicles in good donors by correlating with the frequency and functionality of chondrocyte progenitors. CD271(+)CD51(+) stromal cell subset was found to possess enhanced hMSC activity, with PALLADIN-negative cells displaying the greatest CFU-F potential in subsets enriched for greater chondrogenic ability. The findings contribute to better understanding and isolation of ossicle-forming hMSCs for optimized engineering of human bone organs.
STEM CELLS AND DEVELOPMENT
(2021)
Review
Biochemistry & Molecular Biology
Charindu K. I. Ranmuthu, Chanuka D. S. Ranmuthu, Chalukya K. Wijewardena, Matthew K. T. Seah, Wasim S. Khan
Summary: This systematic review explores the effect of hypoxia on chondrogenic differentiation of human adult mesenchymal stromal cells (MSCs). Most studies suggest that hypoxia during the expansion or differentiation phase enhances some markers of chondrogenic differentiation in adult MSCs. However, results are inconsistent and not always confirmed at the protein level. Factors such as MSC source, donor variability, hypoxia level, exposure time, and culture methodology contribute to these inconsistent findings.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Shanshan Ma, Jiankang Zhou, Tuanjie Huang, Zhenkun Zhang, Qu Xing, Xinkui Zhou, Kun Zhang, Minghao Yao, Tian Cheng, Xiaofeng Wang, Xuejun Wen, Fangxia Guan
Summary: Stem cell therapy holds promise for treating traumatic brain injuries, but low survival and differentiation rates of transplanted cells are obstacles. A hydrogel containing SDF-1, collagen, and sodium alginate was shown to provide a favorable environment for bone marrow-derived stem cells in vitro and improve motor and cognitive function in a rat TBI model. The hydrogel also reduced brain lesions, cell death, and inflammation, suggesting it may be a potential tool for neural repair in brain injury.
ACTA BIOMATERIALIA
(2021)
Article
Cell & Tissue Engineering
Bing-Yu Zhang, Pu Xu, Qing Luo, Guan-Bin Song
Summary: By adding TGF-beta 1 in a collagen sponge-based 3D culture system, the proliferation and tenogenic differentiation of rat BMSCs can be enhanced. The 3D culture significantly improved the differentiation of BMSCs into tenocytes compared to 2D culture, with TGF-beta 1 playing a crucial role in this process.
WORLD JOURNAL OF STEM CELLS
(2021)
Review
Cell Biology
Stefania Crippa, Ludovica Santi, Margherita Berti, Giada De Ponti, Maria Ester Bernardo
Summary: The human organism requires the production of approximately 1 trillion new blood cells per day through hematopoiesis in the bone marrow, regulated by the BM microenvironment and HSPCs. The BM niche, defined by interactions between HSPCs and non-hematopoietic cells, controls HSPC maintenance and orchestrates hematopoiesis according to the body's needs. Mesenchymal stromal cells (MSCs) play a key role in the BM niche by regulating HSPC homeostasis and impacting the outcome of hematopoietic stem cell transplantation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Erwin Pavel Lamparelli, Maria Camilla Ciardulli, Valentina Giudice, Pasqualina Scala, Rosa Vitolo, Tina Patricia Dale, Carmine Selleri, Nicholas Robert Forsyth, Nicola Maffulli, Giovanna Della Porta
Summary: This study investigated the chondrogenic potentials of 3D high-density cultures of bone marrow and Wharton's Jelly-derived mesenchymal stromal cells (MSCs) supplemented with hTGF-beta 1. The study found that high-density cultures promoted chondrogenesis and that Wharton's Jelly-derived MSCs showed potential for chondrogenic commitment. These findings have important implications for cartilage regeneration and tissue engineering.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell & Tissue Engineering
Rose Ann G. Franco, Eamonn McKenna, Pamela G. Robey, Ross W. Crawford, Michael R. Doran, Kathryn Futrega
Summary: For the use of bone marrow stromal cells (BMSC) in cartilage repair, controlling their hypertrophic differentiation is crucial. TGF-beta 1 stimulation triggers chondrogenic and hypertrophic differentiation in BMSCs, and silencing SP7, a transcription factor associated with hypertrophic differentiation, reduces both hypertrophic and chondrogenic differentiation processes. However, SP7 silencing compromises overall trilineage differentiation of BMSCs and makes it challenging to promote chondrogenesis while reducing hypertrophy in cartilage tissue engineering.
JOURNAL OF TISSUE ENGINEERING
(2023)
Article
Cell & Tissue Engineering
K. Futrega, E. Music, P. G. Robey, S. Gronthos, R. Crawford, S. Saifzadeh, T. J. Klein, M. R. Doran
Summary: The study characterized ovine BMSC and evaluated their ability to repair cartilage defects, concluding that micro-pellets manufactured from ovine articular chondrocytes were more effective than those from ovine BMSC. While ovine BMSC could form cartilage-like tissue in vitro, their effective application in cartilage repair will require successful mitigation of hypertrophy and tissue integration issues.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Jean-Claude Imber, Andrea Roccuzzo, Alexandra Staehli, Nikola Saulacic, James Deschner, Anton Sculean, Dieter Daniel Bosshardt
Summary: The study demonstrates a positive effect of VCMX on angiogenesis and suggests an equally high cell turnover in the regenerated and pristine PDL.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Chemistry, Medicinal
Madhan Jeyaraman, Sathish Muthu, Prakash Gangadaran, Rajni Ranjan, Naveen Jeyaraman, Gollahalli Shivashankar Prajwal, Prabhu Chandra Mishra, Ramya Lakshmi Rajendran, Byeong-Cheol Ahn
Summary: The periosteum, rich in pluripotent stem cells, plays a crucial role in fracture healing and remodeling. While its potential in tissue engineering is recognized, further research is needed to explore the bone-forming capabilities of its stem cells.
Article
Biology
Victor Sevastianov, Yulia B. Basok, Ludmila A. Kirsanova, Alexey M. Grigoriev, Alexandra D. Kirillova, Evgeniy A. Nemets, Anastasia M. Subbot, Sergey Gautier
Summary: The study compared the ability of MSCs from human adipose tissue to differentiate into chondrocytes in vitro and stimulate articular cartilage regeneration in a rabbit knee osteoarthrosis model. The findings demonstrated that MCH is more conducive to stimulating cartilage repair by MSCs, while DPC acts as an inducer for MSCs to form cartilage-like tissue in vitro.
Review
Cell & Tissue Engineering
Shanshan Du, Dimitrios Zeugolis, Timothy O'Brien
Summary: Foot ulceration is a serious complication of diabetes mellitus, causing significant burden on individuals and healthcare systems. Impaired wound healing in diabetic patients can be attributed to various factors, including high blood sugar, poor blood vessel formation, and nerve damage. The use of mesenchymal stromal cells (MSCs) has shown promise in improving delayed wound healing, but the direct injection method has limitations.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Polymer Science
Elena Gabusi, Enrico Lenzi, Cristina Manferdini, Paolo Dolzani, Marta Columbaro, Yasmin Saleh, Gina Lisignoli
Summary: Autophagy is a cellular process that maintains cell homeostasis by activating a specific pathway and providing necessary factors in stressful and physiological situations. In a 3D hydrogel, adipose-derived mesenchymal stromal cells (ASCs) showed increased expression of autophagic markers and features. Autophagy plays a crucial role in ASCs chondrogenic differentiation by providing energy and necessary factors.
Article
Cell Biology
Mary Mohrin, Justin Liu, Jose Zavala-Solorio, Sakshi Bhargava, John Maxwell Trumble, Alyssa Brito, Dorothy Hu, Daniel Brooks, Georgios Koukos, Lama Alabdulaaly, Jonathan S. Paw, Kayley Hake, Ganesh Kolumam, Mary L. Bouxsein, Roland Baron, Yuliya Kutskova, Adam Freund
Summary: PAPP-A deficiency modulates bone marrow homeostasis by regulating the number and activity of mesenchymal stromal cells (MSCs), which are not only the major source of extracellular matrix (ECM) but also play essential roles in the development of adult bone, regulation of marrow adiposity, and formation of the hematopoietic niche. This mechanism may contribute to the extended longevity and healthspan associated with PAPP-A deletion.
Article
Computer Science, Interdisciplinary Applications
Tao Liu, Nadr Jomha, Samer Adeeb, Marwan El-Rich, Lindsey Westover
Summary: This study used finite element modeling to evaluate the contact characteristics of three types of cobalt-chrome talus implants in different postures and compared them with a reference biological model. The results showed that biological models had the largest contact areas and smallest peak contact pressures, while implant models had smaller contact areas and relatively larger peak contact pressures. Customized-scale models had a larger total contact area compared to the other two models.
MEDICAL & BIOLOGICAL ENGINEERING & COMPUTING
(2022)
Review
Cell Biology
Zhiyao Ma, Margaret J. Vyhlidal, David Xinzheyang Li, Adetola B. Adesida
Summary: This article reviews various mechanical stimuli applied in meniscus bioengineering over the past 10 years. By evaluating the effects of mechanical stimulation on bioengineered meniscus at both molecular and tissue levels, it provides an important foundation for meniscus tissue engineering and osteoarthritis modeling.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Orthopedics
Kezhou Wu, Kar Wey Yong, Maha Ead, Mark Sommerfeldt, Tamara D. Skene-Arnold, Lindsey Westover, Kajsa Duke, Leila Laouar, Janet A. W. Elliott, Nadr M. Jomha
Summary: This study aimed to investigate whether the repair effect of vitrified particulated cartilage would be equivalent to or better than that of fresh cartilage. The experimental results showed that both fresh and vitrified particulated cartilage had equivalent regeneration within cartilage defects when evaluated at 6 months after surgery.
AMERICAN JOURNAL OF SPORTS MEDICINE
(2022)
Review
Engineering, Biomedical
Xiaoyi Lan, Adetola Adesida, Yaman Boluk
Summary: This article discusses the impact of rheological properties of collagen hydrogels on micro-extrusion and layer-stacking stages of bioprinting. It emphasizes the importance of steady shear flow measurements and large amplitude oscillatory shear tests in evaluating the performance of collagen networks during bioprinting. The article also introduces the significance of non-Newtonian material functions such as first normal stress difference and extensional viscosity. The discussions on collagen networks can also apply to the bioprinting of other hydrogels.
BIOMEDICAL MATERIALS
(2022)
Article
Biology
Kar Wey Yong, Kezhou Wu, Janet A. W. Elliott, Nadr M. Jomha
Summary: This study investigated the improvement of a vitrification protocol for articular cartilage using sucrose as a cryoprotective agent and clinical grade chondroitin sulfate and ascorbic acid as antioxidants. The results showed that sucrose supplementation significantly improved chondrocyte viability after warming, and clinical grade chondroitin sulfate and ascorbic acid showed similar results to their research grade counterparts. The findings suggest that the sucrose-supplemented protocol could be suitable for clinical applications.
Article
Biophysics
Junran Sun, Lindsey Westover, Kezhou Wu, Leila Laouar, Nadr M. Jomha, Samer Adeeb, Gail M. Thornton
Summary: This study compared the mechanical properties of fresh, frozen, and vitrified porcine lateral menisci. The results showed that freezing had detrimental effects on the mechanical properties of menisci, while vitrification preserved mechanical properties comparable to fresh menisci.
JOURNAL OF BIOMECHANICS
(2022)
Article
Cell Biology
Junran Sun, Lindsey Westover, Kezhou Wu, Leila Laouar, Nadr M. Jomha, Samer Adeeb, Gail M. Thornton
Summary: The common practice of freezing meniscal allograft tissue is limited due to the formation of damaging ice crystals. Vitrification, on the other hand, eliminates the formation of ice crystals and allows the mechanical properties of meniscal allograft tissue to be maintained. This study aimed to compare the compressive mechanical properties of fresh, frozen, and vitrified porcine lateral menisci. The results showed that frozen menisci had inferior mechanical properties compared to fresh and vitrified menisci. However, fresh and vitrified menisci exhibited similar compressive mechanical properties. These findings highlight the importance of vitrification for preserving meniscal allograft tissue and suggest that freezing should be used with caution in future investigations.
CELL AND TISSUE BANKING
(2023)
Article
Multidisciplinary Sciences
Zhiyao Ma, David Xinzheyang Li, Ryan K. W. Chee, Melanie Kunze, Aillette Mulet-Sierra, Mark Sommerfeldt, Lindsey Westover, Daniel Graf, Adetola B. Adesida
Summary: Osteoarthritis primarily affects mechanical load-bearing joints, with the knee being the most common. Knee osteoarthritis (KOA) is more prevalent, burdensome, and severe in females, and hormonal differences do not fully explain this. Mechanical unloading in spaceflight microgravity has been implicated in the development of arthritis, but the mechanisms and sex differences are unclear. In this study, engineered meniscus constructs were generated and compared between male and female donors under normal gravity and simulated microgravity conditions to investigate the molecular basis and sex differences in the development of KOA.
Article
Multidisciplinary Sciences
A. K. Aissiou, S. Jha, K. Dhunnoo, Z. Ma, D. X. Li, R. Ravin, M. Kunze, K. Wong, A. B. Adesida
Summary: Spaceflight or simulated spaceflight microgravity can induce osteoarthritic-like alterations in the articular and meniscal cartilages of rodents, but its effect on tissue-engineered cartilage developed from human cells is not well understood. This study investigates the response of tissue-engineered cartilage to simulated microgravity facilitated by parabolic flights and reveals that the transcriptome profile and secretome of the engineered cartilage tissues showed sex-dependent differences in their modulation. These findings provide a foundation for further exploration of microgravity's underlying mechanisms.
Article
Engineering, Biomedical
Xiaoyi Lan, Zhiyao Ma, Melanie Kunze, Aillette Mulet-Sierra, Martin Osswald, Khalid Ansari, Hadi Seikaly, Yaman Boluk, Adetola B. Adesida
Summary: Hydrogels with different crosslinking densities were used to investigate their effects on chondrocyte phenotype and cellular interaction. The results showed that the storage modulus of the hydrogel did not significantly alter the cartilaginous gene expression of chondrocytes. However, the gene expression of PPAR-gamma was positively correlated with crosslinking density. RNA-seq analysis revealed numerous genes that were significantly correlated to crosslinking density, which warrants further investigation.
ANNALS OF BIOMEDICAL ENGINEERING
(2023)
Article
Engineering, Biomedical
Xiaoyi Lan, Yaman Boluk, Adetola B. Adesida
Summary: Due to the limited self-repair capacity of hyaline cartilage, tissue engineering strategy with 3D bioprinting technique has emerged as a new solution for hyaline cartilage repair and regeneration. This review summarizes the intrinsic property of hyaline cartilage and discusses the selection of bioprinters, bioink materials, and cell sources for successful bioprinting. The aim of this review is to identify current challenges and future directions for clinical development of bioprinted hyaline cartilage.
ANNALS OF BIOMEDICAL ENGINEERING
(2023)
Article
Engineering, Biomedical
Maha Ead, Kezhou Wu, Chester Jar, Kajsa Duke, Nadr Jomha, Lindsey Westover
Summary: Osteochondral allograft transplantations are commonly used for treating focal articular cartilage injuries. Vitrification, a cryopreservation method, can extend the storage time of cartilage. This study found that vitrified cartilage had similar or better mechanical properties compared to fresh and frozen cartilage, suggesting it can be an alternative to fresh allografts and potentially improve clinical outcomes.
ANNALS OF BIOMEDICAL ENGINEERING
(2023)
Article
Orthopedics
Mary Crisol, Kezhou Wu, Barry Congdon, Tamara D. Skene-Arnold, Leila Laouar, Janet A. W. Elliott, Nadr M. Jomha
Summary: This study evaluated the chondrocyte viability of post-vitrified particulated articular cartilage during a 7-day tissue storage period at 4 degrees C. The results showed that the cell viability of the cryopreserved cartilage remained above 80%, which is acceptable for clinical translation.
Article
Cell Biology
Noor Abdelwahab, Mohammadhamed Shahsavari, Kezhou Wu, Leila Laouar, Tamara D. Skene-Arnold, Janet A. W. Elliott, Nadr M. Jomha
Summary: Osteochondral allograft transplantation is a successful method for repairing articular cartilage defects and preventing osteoarthritis. This study compared three tissue/cell media for storing cartilage tissue and found that all three were equally effective in maintaining cell viability. The use of lower cost cell media (DMEM/F12 and RPMI-1640) is therefore recommended for hypothermic storage of articular cartilage tissue.
CELL AND TISSUE BANKING
(2023)
Article
Cell & Tissue Engineering
David Xinzheyang Li, Zhiyao Ma, Alexander R. A. Szojka, Xiaoyi Lan, Melanie Kunze, Aillette Mulet-Sierra, Lindsey Westover, Adetola B. Adesida
Summary: This study introduced a novel protocol to differentiate hBM-MSC into stable, cartilage-forming cells. By combining mechano-hypoxia conditioning with TGF-beta 3 growth factor withdrawal, non-hypertrophic chondrogenesis of hBM-MSC can be promoted.
JOURNAL OF TISSUE ENGINEERING
(2023)