Article
Multidisciplinary Sciences
Zhuhao Wu, Lingyu Sun, Hanxu Chen, Yuanjin Zhao
Summary: This paper presents a novel acoustic approach for fabricating biomimetic surfaces that can manipulate droplets. The approach utilizes capillary waves induced by surface acoustic waves to deform polymer films into pre-designed structures, enabling impressive droplet transportation and water collection abilities. Additionally, the tunability of acoustics allows for dynamic controllability, such as programmable wettability, mitigating droplet evaporation, and accelerating chemical reactions. This approach simplifies the fabrication process and eliminates the need for additional chemical modifications, providing unique perspectives for various applications including microreactor integration, biochemical reaction control, and tissue engineering.
Article
Developmental Biology
Simon Berger, Silvan Spiri, Andrew DeMello, Alex Hajnal
Summary: A novel microfluidic imaging method has been introduced to allow parallel live-imaging across multiple larval stages of Caenorhabditis elegans while maintaining worm orientation and identity. The method ensures stable worm orientation and viability, showcasing capabilities such as observing hypodermal seam and P-cell divisions, as well as the entire process of vulval development, and demonstrating feasibility of on-chip RNAi.
Article
Chemistry, Multidisciplinary
William Sean Harley, Kirill Kolesnik, Mingxin Xu, Daniel Edward Heath, David John Collins
Summary: This work demonstrates a method to generate designed and highly localized acoustic fields using 3D resonant mass-spring microstructures, enabling rapid and spatially defined controllable micromanipulation. This sub-wavelength, 3D acoustofluidic approach has potential applications in sample preparation, cell analysis, and diagnostics.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Analytical
Vittorio Viri, Mael Arveiler, Thomas Lehnert, Martin Gijs
Summary: The study utilized PDMS microfluidic device to investigate bacteria in the gut of C. elegans, revealing the digestion process of bacteria in the worm's intestine through time-lapse imaging and high-resolution fluorescence imaging. The results suggest the potential for performing infection assays with this platform.
Article
Acoustics
Jing Dong, Dongfang Liang, Xin Yang, Chao Sun
Summary: The study focuses on the acoustophoresis of microparticles in a compressible liquid, with emphasis on the efficiency of particle accumulation depending on microchannel height, and an extensive parametric study was conducted to identify the optimum microchannel height. The findings provide insights into the design of acoustophoretic devices.
Article
Chemistry, Analytical
Sungryul Park, Sangwook Lee, Hyun Soo Kim, Hong Jin Choi, Ok Chan Jeong, Ruixian Lin, Younghak Cho, Min-Ho Lee
Summary: The article introduces a simple on-chip acoustofluidic method to align ellipsoidal cells and focus them into a single stream. By generating acoustic standing waves in a microchannel, the method successfully aligns and focuses a population of Euglena gracilis cells. This method is important for the application of flow cytometry.
Article
Acoustics
Yuwen Li, Wei Tan, Zhifang Liu, Shuoshuo Mu, Guorui Zhu
Summary: This paper presents experimental investigations on acoustic streaming in viscoelastic fluids for the first time. The addition of polyethylene oxide (PEO) polymer significantly alters the flow characteristics in the microchannel, resulting in two modes of acousto-elastic flow: positive mode and negative mode. The viscoelastic fluids exhibit mixing hysteresis features at low flow rates and flow pattern degeneration at high flow rates. The degeneration of flow pattern is further summarized as time fluctuation and spatial disturbance range reduction. The positive mode in acousto-elastic flow can enhance the mixing of viscoelastic fluids in micromixers, while the negative mode provides a potential method for particle/cell manipulation in viscoelastic body fluids such as saliva by suppressing unstable flow.
ULTRASONICS SONOCHEMISTRY
(2023)
Review
Biochemical Research Methods
Jakub Novotny, Andreas Lenshof, Thomas Laurell
Summary: Acoustofluidics, the field that involves the interaction of ultrasonic standing waves with fluids and microparticles, has gained increasing interest in microfluidic applications. Integrated systems have been developed for contactless manipulation of cells and particles based on acoustic standing waves, with studies showing the beneficial effects of acoustic forces on cells in biomaterial manipulation.
Article
Chemistry, Multidisciplinary
Reza Rasouli, Maryam Tabrizian
Summary: 3D cell spheroid culture provides a faithful recreation of cell growth environment compared to 2D culture, with a new boundary-driven acoustic microstreaming tool that accelerates spheroid production, showing versatility in producing different types of spheroids.
Article
Biology
Taslim Anupom, Siva A. Vanapalli
Summary: This study introduces a 3D printed compact imaging platform (CIP) integrated with a smart-device camera for whole-organism phenotyping of C. elegans. The CIP simplifies the optical configuration and can be easily operated and controlled. It shows good compatibility with different C. elegans substrates and accurately measures phenotypic data. The compactness, portability, and versatility of the CIP make it desirable for research and educational outreach applications on Earth and in space.
Article
Chemistry, Analytical
Tingting Zhu, Lanxin Zhu, Yi Li, Xiaopeng Chen, Mingyang He, Guo Li, Hao Zhang, Shangbang Gao, Peng Fei
Summary: The novel fusion of microfluidics and light-field microscopy allows for high-speed 4D imaging of moving C. elegans, providing a new approach for studying neural activities.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biotechnology & Applied Microbiology
Zhuhao Wu, Zheng Ao, Hongwei Cai, Xiang Li, Bin Chen, Honglei Tu, Yijie Wang, Rongze Olivia Lu, Mingxia Gu, Liang Cheng, Xin Lu, Feng Guo
Summary: Cancer immunotherapy has potential for treating breast cancer, but predicting individual patient's response is challenging. Researchers developed a new method using acoustofluidic technology to assemble cell clusters from patient primary breast tumor dissociation within 2 minutes, allowing rapid and reliable evaluation of immunotherapy. This method offers a new approach for personalized cancer therapy.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Wan-Hsuan Lin, Chien-Wei Chen, Sheng-Hang Wang, Bor-Ran Li
Summary: This paper presents a simple and fast superhydrophobic modification and patterning approach for manipulating droplets to achieve transport, mixing, concentration, and rebounding control. The method significantly improves sensitivity and reduces reaction time for bioassays, while also demonstrating potential for expanding applications of open microfluidics. Control of droplet impacting and rebounding phenomena is also discussed for further applications of surface wettability.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Nadia Vertti-Quintero, Simon Berger, Xavier Casadevall i Solvas, Cyril Statzer, Jillian Annis, Peter Ruppen, Stavros Stavrakis, Collin Y. Ewald, Rudiyanto Gunawan, Andrew J. DeMello
Summary: The study demonstrates that protein homeostasis maintenance is a major factor influencing the heterogeneity in HSR dynamics, with individuals with enhanced protein homeostasis fidelity living longer in early adulthood. Age-related decline in protein homeostasis shows a stochastic-onset in day-1 and day-2 adult C. elegans, increasing the heterogeneity in HSR capacity. Additionally, C. elegans embryos exhibit higher HSR and proteostasis capacity compared to young adults, showing a transgenerational contribution to HSR heterogeneity dependent on maternal age.
Article
Chemistry, Analytical
Hanlin Wang, Joe Boardman, Xiaoyan Zhang, Chao Sun, Meng Cai, Jun Wei, Zhiqiang Dong, Mingqian Feng, Dongfang Liang, Sheng Hu, Yu Qian, Shuang Dong, Yongqing Fu, Hamdi Torun, Aled Clayton, Zhenlin Wu, Zhihua Xie, Xin Yang
Summary: An enhanced tilted-angle (ETA) acoustofluidic device is developed for cell mechanophenotyping, which can distinguish and identify different cell types based on their acoustic contrast factors. The device uses an interdigital transducer and introduces a 5-degree inclination angle to manipulate cells according to their mechanical properties. The methodology is demonstrated by distinguishing lung and breast cancer cells from leukocytes.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biophysics
Nanjing Hao, Zeyu Wang, Pengzhan Liu, Ryan Becker, Shujie Yang, Kaichun Yang, Zhichao Pei, Peiran Zhang, Jianping Xia, Liang Shen, Lin Wang, Kathleen A. Welsh-Bohmer, Laurie Sanders, Luke P. Lee, Tony Jun Huang
Summary: Alzheimer's disease is a severe neurodegenerative disorder with early diagnosis being a challenge. Researchers have developed an integrated diagnostic system combining acoustics, microfluidics, and biosensors for accurate and rapid detection of AD biomarkers, showing promising results for early diagnosis.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Optics
Zijie Zhao, Jianping Xia, Tony Jun Huang, Jun Zou
Summary: This Letter presents a ring-shaped photoacoustic tweezers capable of manipulating micron-sized particles. By illuminating an absorptive liquid medium with a pulsed laser beam, acoustic radiation force and vaporization repulsion are generated, enabling continuous and versatile movement of single or multiple particles. The tweezers demonstrate interactions between particles, including separation, attachment, and grouping, combining the advantages of optical and acoustic tweezers.
Article
Chemistry, Physical
Shujie Yang, Zhenhua Tian, Zeyu Wang, Joseph Rufo, Peng Li, John Mai, Jianping Xia, Hunter Bachman, Po-Hsun Huang, Mengxi Wu, Chuyi Chen, Luke P. Lee, Tony Jun Huang
Summary: The authors introduce a harmonic acoustics platform for non-contact and dynamic particle manipulation. This method enables precise assembly of colloidal crystals or cells and measurement of intercellular adhesion forces, with subwavelength resolution and high throughput.
Article
Multidisciplinary Sciences
Pengzhan Liu, Zhenhua Tian, Kaichun Yang, Ty Downing Naquin, Nanjing Hao, Huiyu Huang, Jinyan Chen, Qiuxia Ma, Hunter Bachman, Peiran Zhang, Xiahong Xu, Junhui Hu, Tony Jun Huang
Summary: Acoustofluidic black holes (AFBHs) are a technology that can slow down and capture acoustic waves, enabling versatile particle manipulation functionalities. This study fills the gap between acoustofluidics and acoustic black holes, leading to potential applications in various fields.
Article
Nanoscience & Nanotechnology
Zeyu Wang, Joseph Rich, Nanjing Hao, Yuyang Gu, Chuyi Chen, Shujie Yang, Peiran Zhang, Tony Jun Huang
Summary: Researchers have developed an acoustofluidic device that enables simultaneous drug loading and exosome encapsulation. This device utilizes acoustic forces to concentrate and fuse drugs and nanoparticles, resulting in drug-loaded silica nanocarriers encased within an exosomal membrane. The drug loading efficiency is significantly improved, and the process can be completed within minutes, without the need for complex chemical modification.
MICROSYSTEMS & NANOENGINEERING
(2022)
Article
Multidisciplinary Sciences
Joseph Rufo, Peiran Zhang, Ruoyu Zhong, Luke P. Lee, Tony Jun Huang
Summary: Newly developed acoustic technologies have the potential to address critical challenges in biomedicine, such as activation of ion channels and development of biofabrication protocols.
NATURE COMMUNICATIONS
(2022)
Article
Robotics
Murat Kaynak, Amit Dolev, Mahmut Selman Sakar
Summary: This study demonstrates that programmed commands can be contained on 3D nanoprinted polymer systems by introducing selectively excited air bubbles and rationally designed compliant mechanisms. The dynamics of bubble oscillators reveal rich acoustofluidic interactions that can be programmed in space and time.
Article
Nanoscience & Nanotechnology
Geonsoo Jin, Joseph Rich, Jianping Xia, Albert J. He, Chenglong Zhao, Tony Jun Huang
Summary: Combining nanoscale optical resolution with a large field of view is crucial in many research and industry areas. To overcome limitations of existing scanning microscopes, a dual-camera acoustofluidic nanoscope has been developed, allowing for precise imaging of samples and microspheres simultaneously. This innovation reduces the number of images needed for capturing the entire field of view by 55-fold compared to previous designs, while greatly improving image quality through an alpha-blending imaging technique.
MICROSYSTEMS & NANOENGINEERING
(2022)
Article
Materials Science, Multidisciplinary
Xu Zhang, Ye He, Xuan Li, Chuanchuan Lin, Zhang Yuan, Liangliang Dai, Feng Ma, Yi Lv, Tony Jun Huang, Mudan Ren, Kaiyong Cai, Shuixiang He
Summary: This study reports a hybrid hydrogel for the treatment of gastric ulcers induced by endoscopic submucosal dissection (ESD). The hydrogel, loaded with drug-carrying nanoparticles, showed unique stability in gastric tissue and released the drug slowly, inducing polarization of macrophages and promoting gastric ulcer healing in an in vivo model.
APPLIED MATERIALS TODAY
(2022)
Article
Engineering, Mechanical
Adem Ozcelik
Summary: The study demonstrates the feasibility of using 3D printing to fabricate microfluidic devices for lab-on-a-chip applications. The printed devices achieved continuous and efficient separation of target cells with high purity and cell recovery rates.
Article
Nanoscience & Nanotechnology
Zirui Liu, Tieyi Li, Zeyu Wang, Jun Liu, Shan Huang, Byoung Hoon Min, Ji Young An, Kyoung Mee Kim, Sung Kim, Yiqing Chen, Huinan Liu, Yong Kim, David T. W. Wong, Tony Jun Huang, Ya-Hong Xie
Summary: This study reports the potential of using surface-enhanced Raman spectroscopy (SERS) to detect gastric cancer through the composition information of Raman-active bonds inside small extracellular vesicles (sEVs). A machine learning-based spectral feature analysis algorithm was developed to objectively distinguish cancer-derived sEVs from non-cancer sub-population sEVs.
ACS APPLIED NANO MATERIALS
(2022)
Article
Chemistry, Analytical
Fatih Akkoyun, Adem Ozcelik, Ibrahim Arpaci, Ali Ercetin, Sinan Gucluer
Summary: Egg classification can be achieved using image processing technology, which enables fast and cost-effective classification by obtaining and analyzing real-time images. This method has been successfully applied in an industrial production line.
Article
Chemistry, Analytical
Adem Ozcelik, Burcin Irem Abas, Omer Erdogan, Evrim Cevik, Ozge Cevik
Summary: In this study, simple and low-cost microfluidic devices were designed and fabricated to develop an organoid model for drug testing for renal cancers. The results showed that the standard renal cancer organoids made on a lab-on-a-chip system can be used to measure drug effects and tumor microenvironment responses.
Article
Chemistry, Multidisciplinary
Cornel Dillinger, Justin Knipper, Nitesh Nama, Daniel Ahmed
Summary: The manipulation of soft polymeric microrobots in aquatic environments is challenging, but wireless manipulation can be achieved using external field-guided technologies. This study presents the fabrication and manipulation of a magnetized soft polymeric microrobot, which achieves stable manipulation through acoustic propulsion and magnetic field-guided navigation.
Article
Physics, Fluids & Plasmas
Khemraj Gautam Kshetri, Nitesh Nama
Summary: Acoustofluidic systems utilize prefabricated acoustic scatterers to perturb the imposed acoustic field, which enables the acoustophoresis of immersed microparticles. This numerical study investigates the streaming and radiation force fields around a scatterer and identifies a critical transition size at which the motion of microparticles shifts from being dominated by streaming to being dominated by radiation. The study also reveals that the critical transition size decreases with increasing acoustic frequency, and it is affected by the scatterer material and fluid properties.
