Article
Chemistry, Multidisciplinary
Tianwen Li, Liansheng Zhang, Tao Lu, Tongming Zhu, Canbin Feng, Ni Gao, Fei Liu, Jingyu Yu, Kezhu Chen, Junjie Zhong, Qisheng Tang, Quan Zhang, Xiangyang Deng, Junwei Ren, Jun Zeng, Haibo Zhou, Jianhong Zhu
Summary: This study developed an optimized signal peptide and engineered extracellular vesicles for delivering RNA editing tools, and demonstrated their efficacy in inhibiting gene expression and treating acute inflammatory diseases in vitro and in vivo.
Review
Microbiology
Lixin Zhao, Minyue Qiu, Xiaojia Li, Juanzhen Yang, Jintao Li
Summary: The CRISPR/Cas13a system, as a natural adaptive immune system, has been widely studied for its applications in molecular diagnostics, gene therapy, gene editing, and RNA imaging. It has great potential in the field of molecular diagnostics, with applications in pathogen detection, biomarker detection, and non-nucleic acid target detection.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hong Mei, Qian Gu, Wei Wang, Yu Meng, Lichun Jiang, Jia Liu
Summary: CRISPR-Surfaceome is an online tool and database for designing highly efficient sgRNA to knockout human cell surface proteins. The sgRNAs designed by this tool can be used for functional genomics studies, such as identification of pathogen receptors and discovery of drug targets.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Review
Chemistry, Multidisciplinary
Vanja Perculija, Jinying Lin, Bo Zhang, Songying Ouyang
Summary: CRISPR-Cas systems are a form of prokaryotic adaptive immunity that use RNA-guided endonucleases to cleave foreign genetic elements, with great potential for application in research, biotechnology, and therapeutics. While DNA-targeting Cas effectors have been widely used in genome editing, the recent discovery of RNA-targeting CRISPR-Cas systems has shown promise for RNA manipulation as well.
Article
Immunology
Xiaotong Qiu, Shuai Xu, Xueping Liu, Hongtao Ren, Lichao Han, Zhenjun Li
Summary: Under the COVID-19 pandemic background, a nucleic acid detection platform called CRISPR-CLA was developed, which combines CRISPR/Cas12a-based detection with loop-mediated isothermal amplification (LAMP). This platform offers rapid, low-cost, and reliable nucleic acid detection, and has been successfully applied for molecular diagnosis of Nocardia farcinica infection with high specificity compared to real-time qPCR method.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Chunjing Li, Yu Cao, Li Zhang, Jierong Li, Jianfeng Wang, Yanfen Zhou, Huiling Wei, Mingjuan Guo, Liang Liu, Chunxiao Liu, Shilin Zhang, Guoqing Liu
Summary: The study revealed that LINC00341 is overexpressed in bladder cancer tissues compared to healthy tissues, with higher levels in high-grade tumors. Inhibition of LINC00341 resulted in reduced cell proliferation and migration, and increased apoptosis. Additionally, silencing LINC00341 upregulated the expressions of p21, Bax, and E-cadherin.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Cell Biology
Xabier Osteikoetxea, Andreia Silva, Elisa Lazaro-Ibanez, Nikki Salmond, Olga Shatnyeva, Josia Stein, Jan Schick, Stephen Wren, Julia Lindgren, Mike Firth, Alexandra Madsen, Lorenz M. Mayr, Ross Overman, Rick Davies, Niek Dekker
Summary: This study achieves efficient gene editing by loading CRISPR/Cas9 into EVs through reversible heterodimerization, demonstrating the great potential of EVs in gene delivery.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Article
Multidisciplinary Sciences
Jianhai Chen, Silue Zeng, Qiang Xue, Yuchuan Hong, Liangjian Liu, Liang Song, Chihua Fang, Hai Zhang, Benguo Wang, Adam C. Sedgwick, Peng Zhang, Jonathan L. Sessler, Chengbo Liu, Jingqin Chen
Summary: This study presents a new strategy for the diagnosis, treatment, and monitoring of rheumatoid arthritis (RA) by using macrophage biomimetic nanoparticles and small interfering RNA (siRNA) to regulate the RA microenvironment. The siRNA is able to silence proinflammatory cytokine expression, while the nanoparticles scavenge reactive oxygen species (ROS). Near-infrared photoacoustic (PA) imaging allows real-time monitoring and evaluation of the treatment. Overall, this approach shows potential for improving the management of RA.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Ophthalmology
Satheesh Kumar, Lewis E. Fry, Jiang-Hui Wang, Keith R. Martin, Alex W. Hewitt, Fred K. Chen, Guei-Sheung Liu
Summary: Genetic medicine provides hope for previously untreatable diseases, and the eye is at the forefront of these advances. Luxturna(R) is an example of a gene therapy that gained US FDA approval for the treatment of inherited blindness, and other gene therapy clinical trials for eye diseases are ongoing. RNA-targeting systems, such as antisense oligonucleotides (ASO) and RNA interference (RNAi), as well as CRISPR-Cas systems, are emerging as promising strategies for developing therapeutics.
PROGRESS IN RETINAL AND EYE RESEARCH
(2023)
Article
Genetics & Heredity
Mingxing Lu, Zuowei Wang, Yixiu Wang, Bingbing Ren
Summary: This article summarizes the latest advances in CRISPR-guided proximity labeling in studying RNA-protein interactions, and discusses the possibility of applying engineered proximity-labeling enzymes to study RNA-centric interactions in the future.
Article
Biochemistry & Molecular Biology
Sandra Gawlitt, Chunyu Liao, Tatjana Achmedov, Chase L. Beisel
Summary: CRISPR technologies can utilize multiple gRNAs to enact multi-site editing or regulation. Researchers have found that CRISPR arrays utilized by the Cas9 nuclease can be shortened without compromising and sometimes even enhancing targeting activity. Shortened CRISPR-Cas9 arrays can facilitate multiplexed editing and gene regulation from a smaller DNA footprint across many bacterial applications of CRISPR technologies.
Article
Medicine, Research & Experimental
Lin Wang, Junhu Zhou, Qixue Wang, Yunfei Wang, Chunsheng Kang
Summary: This study proposes a potential anti-SARS-CoV-2 strategy based on the CRISPR-Cas13a system, identifying a specific RNA segment of the spike protein and designing an efficient crRNA sequence for cleavage. It provides a rapid design pipeline for an antiviral tool against SARS-CoV-2 and introduces a novel approach for anti-virus study.
Article
Biochemistry & Molecular Biology
Andras Talas, Krisztina Huszar, Peter Istvan Kulcsar, Julia K. Varga, Eva Varga, Eszter Toth, Zsombor Welker, Gergely Erdos, Peter Ferenc Pach, Agnes Welker, Zoltan Gyorgypal, Gabor E. Tusnady, Ervin Welker
Summary: Detailed target-selectivity information and experiment-based efficacy prediction tools are primarily available for Streptococcus pyogenes Cas9 (SpCas9). One obstacle to develop such tools is the rarity of accurate data. The researchers have developed a method called 'Self-targeting sgRNA Library Screen' (SLS) to analyze the activity of Cas9 nucleases in bacteria, providing a more affordable and efficient alternative to current approaches. The algorithm created for predicting the activity of SpCas9-HF1 matches the accuracy of existing tools.
NUCLEIC ACIDS RESEARCH
(2021)
Review
Cell Biology
Martyna Kordys, Raneet Sen, Zbigniew Warkocki
Summary: CRISPR-Cas13 is a highly versatile prokaryotic defense system that has been widely used in killing antibiotic-resistant bacteria, developing highly sensitive detection tools, and regulating gene expression. Additionally, Cas13 can be applied to in vivo RNA modifications and the detection of RNA-interacting proteins among other applications.
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2022)
Article
Biochemistry & Molecular Biology
Seak Hee Oh, Hye-Jin Lee, Mi Kyoung Ahn, Mi Yeon Jeon, Jeong-Soo Yoon, Yeon Ju Jung, Gyeong-Nam Kim, In-Jeoung Baek, Inki Kim, Kyung Mo Kim, Young Hoon Sung
Summary: CRISPR-Cas systems, such as Cas9 and Cpf1, are effective tools for generating gene knockout mouse models. A hybrid guide RNA system combining Cas9 and Cpf1 has been developed for multiplex gene editing, showing successful results in targeting multiple genes in mouse embryos.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)