Article
Endocrinology & Metabolism
Ziyu Guo, Jihong Yang, Guangping Yang, Ting Feng, Xinyue Zhang, Yao Chen, Ruizhi Feng, Yun Qian
Summary: The study found that higher levels of NAM in large follicles are associated with larger follicle development and higher rates of oocyte maturation and fertilization. Adding 5mM NAM during in vitro maturation process can improve mice oocyte quality and reduce damage caused by oxidative stress.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2022)
Article
Obstetrics & Gynecology
Zhao-Cheng Zeng, Jiang Jiang, Xin-Jie Wang, Kang-Na Wei, Hui-Sheng Liang, Li-Xin Zeng, Ying Xu, Shu-Juan Xie, Zhe Meng, Xiang-Jun Yang, Ai -Wei Guo, Hai-Long Wang
Summary: The results of this study suggest that kaempferol may be a potent natural antioxidant and could alleviate postovulatory oocyte ageing. These findings have implications for animal husbandry and may help improve the success rate of IVF and ICSI.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Cell Biology
Yue Xiao, Bao Yuan, Weiyi Hu, Jiajia Qi, Hao Jiang, Boxing Sun, Jiabao Zhang, Shuang Liang
Summary: Exposure to TBTO prevents porcine oocyte maturation by inducing intracellular ROS accumulation, causing mitochondrial dysfunction, and disrupting cellular iron homeostasis, ultimately decreasing quality and impairing subsequent embryonic developmental competence of porcine oocytes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Endocrinology & Metabolism
Chang Liu, Meng Wang, Haixia Yao, Mengge Cui, Xueqi Gong, Lan Wang, Cong Sui, Hanwang Zhang
Summary: The study found that follicular extracellular vesicles (EVs) of polycystic ovary syndrome (PCOS) patients interfere with the quality of oocytes, leading to abnormal development and maturation. PCOS-EVs cause abnormal distribution of mitochondria and spindles in oocytes, inhibiting oocyte maturation. Moreover, oxidative stress induced by PCOS-EVs may be a potential cause.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Article
Biochemistry & Molecular Biology
Paolo Giovanni Artini, Giorgia Scarfo, Ilaria Marzi, Jonathan Fusi, Maria Elena Obino, Ferdinando Franzoni, Elisa Zappelli, Elisa Chelucci, Claudia Martini, Vito Cela, Simona Daniele
Summary: The molecular/metabolic composition of follicular fluid (FF) has a significant impact on oocyte development and fertilization. The antioxidant potential of FF is correlated with the number of retrieved/mature oocytes and fertilized ones. FF from normal fertilized oocytes exhibits elevated antioxidant capability, lower levels of pro-inflammatory molecules, and higher IL-10 concentration. Samples giving rise to top-quality embryos display a unique antioxidant capability and further decrease in pro-inflammatory molecules compared to FF from poor-quality embryos. Successful embryo implantation is associated with lower pro-inflammatory molecules and high antioxidant capability. Antioxidant capability and IL-10 show good predictive ability for fertilization and embryo quality.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Di Zhang, Jia Lv, Rongxin Tang, Ying Feng, Yongfeng Zhao, Xiaoyang Fei, Richeng Chian, Qigui Xie
Summary: The study investigated exosomal microRNAs in human ovarian follicular fluid to explore their potential association with oocyte quality. Analysis revealed that several exosomal miRNAs were critical for maintaining oocyte quality, suggesting they may be potential biomarkers for evaluating oocyte quality, which is important in clinical settings.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Environmental Sciences
Jingyue Chen, Yilong Miao, Qian Gao, Zhaokang Cui, Bo Xiong
Summary: The study reveals that exposure to PFOS affects the quality of oocytes by disrupting meiotic competency, cytoskeleton assembly, and mitochondrial function, leading to oxidative stress-induced apoptosis.
ENVIRONMENTAL POLLUTION
(2021)
Article
Environmental Sciences
Xiaoxia Yu, Fei Meng, Ju Huang, Weidong Li, Jiaming Zhang, Shen Yin, Liangran Zhang, Shunxin Wang
Summary: This study demonstrated that exposure to 1-NP disrupts mouse oocyte maturation by affecting meiotic spindle assembly, chromosome alignment, first polar body extrusion, fertilization capability, mitochondrial function, intracellular redox balance, and gene expression related to antioxidant enzymes.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Environmental Sciences
Yang-Cheng Yao, Chong Liu, Lin-Jing Wu, Xiao-Qiong Yuan, Yao-Yao Du, Ni-Jie Li, Na Guo, Tao-Ran Deng, Xiang Hua, Xue-Mei Teng, Li Yin, Yu-Feng Li
Summary: The study investigated the associations between medication intake and phthalate metabolites in urine and follicular fluid (FF) among women undergoing IVF. Results showed that the intake of traditional Chinese medicine (TCM) was positively associated with certain phthalate metabolite concentrations, while antioxidant intake was negatively associated with urinary phthalate concentration. Overall, the data suggested that medication intake may impact phthalate exposure in women undergoing IVF.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Medicine, General & Internal
Aleksandra Z. Stojanovic Gavrilovic, Jelena M. Cekovic, Aida Z. Parandilovic, Aleksandar B. Nikolov, Predrag S. Sazdanovic, Aleksandra M. Velickovic, Marija V. Andjelkovic, Marija P. Sorak
Summary: The quality of oocytes is influenced by the microenvironment, including the concentration of interleukin 6 in the follicular fluid. This study analyzed follicular fluid samples from 83 patients undergoing IVF and found a correlation between the concentration of interleukin 6 in the follicular fluid and the outcome of IVF.
Article
Cell Biology
Mianqun Zhang, Lei Sun, Zihao Zhang, Luyan Shentu, Yiwen Zhang, Ziyi Li, Yongteng Zhang, Yunhai Zhang
Summary: Arsenite exposure can negatively impact the quality of oocytes, but supplementation of alpha-lipoic acid (ALA) can restore their competency and fertilization capacity. ALA improves the maturation of oocytes, enhances sperm binding ability, reduces oxidative stress, and prevents DNA damage and apoptosis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sandor Pentek, Akos Varnagy, Balint Farkas, Peter Mauchart, Krisztina Godony, Timea Varjas, Tamas Koszegi, Peter Kaltenecker, Rita Jakabfi-Csepregi, Kalman Kovacs, Jozsef Bodis, Endre Sulyok
Summary: This study aimed to investigate the relationship between telomere length, telomerase activity, and oxidative DNA damage in IVF patients. The study found that telomere lengths were similar in granulosa cells and follicular fluid, but telomerase activity was significantly reduced and 8-OHdG levels were significantly elevated in follicular fluid. Additionally, high levels of 8-OHdG were associated with negative outcomes in IVF success and clinical pregnancies.
Article
Biology
Ju Wang, Wei Zheng, Shuoping Zhang, Keqiang Yan, Miao Jin, Huiling Hu, Zhen Ma, Fei Gong, Guangxiu Lu, Yan Ren, Liang Lin, Ge Lin, Liang Hu, Siqi Liu
Summary: This study established a model for evaluating embryo or oocyte quality based on metabolites in follicular fluid, with phosphatidylcholines identified as key contributors. The increase of PCs in follicular fluid may lead to a decrease in embryo quality. Pathway analysis suggested that accumulation of arachidonic acid induced by PCs could weaken embryo quality.
Article
Genetics & Heredity
Yue Liu, Zhiheng Yu, Shigang Zhao, Lei Cheng, Yuanyuan Man, Xueying Gao, Han Zhao
Summary: Elevated oxidative stress levels were found in both the serum and follicular fluid of PCOS patients compared to normal controls, with the former showing more severe oxidative stress. The study revealed that oxidative stress in follicular fluid has a stronger impact on embryo quality, highlighting the importance of this factor in future research.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Agriculture, Dairy & Animal Science
Yue Han, Xinglin Qu, Xuan Chen, Yanqiu Lv, Yuyang Zhang, Yi Jin
Summary: This study investigates the effect of porcine follicular fluid exosomes on cumulus expansion and oocyte maturation. The results suggest that porcine FF-Exo play a role in oocyte nuclear maturation but do not significantly affect cumulus expansion or mitochondrial membrane potential.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Alice Luddi, Camilla Marrocco, Laura Governini, Bianca Semplici, Valentina Pavone, Stefano Luisi, Felice Petraglia, Paola Piomboni
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Cell Biology
Alice Luddi, Valentina Pavone, Bianca Semplici, Laura Governini, Mattia Criscuoli, Eugenio Paccagnini, Mariangela Gentile, Giuseppe Morgante, Vincenzo De Leo, Giuseppe Belmonte, Natasa Zarovni, Paola Piomboni
Article
Biochemistry & Molecular Biology
Simone Luti, Tania Fiaschi, Francesca Magherini, Pietro A. Modesti, Paola Piomboni, Laura Governini, Alice Luddi, Angela Amoresano, Anna Illiano, Gabriella Pinto, Alessandra Modesti, Tania Gamberi
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2020)
Article
Biochemistry & Molecular Biology
Alice Luddi, Laura Governini, Angela Capaldo, Giovanna Campanella, Vincenzo De Leo, Paola Piomboni, Giuseppe Morgante
Article
Cell Biology
Bianca Semplici, Francesca Paola Luongo, Sofia Passaponti, Claudia Landi, Laura Governini, Giuseppe Morgante, Vincenzo De Leo, Paola Piomboni, Alice Luddi
Summary: Our study examines the expression and localization of bitter taste receptors in somatic ovarian follicle cells, with TAS2R14 being the most highly expressed subtype in cumulus and granulosa cells. We also observed a slight increase in TAS2R14 and TAS2R43 expression in young women, with a potential negative correlation between TAS2R43 expression and oocyte collection. The study provides evidence for the expression of alpha-gustducin and alpha-transducin in cumulus and granulosa cells, with additional isoforms of alpha-gustducin found in granulosa cells.
Article
Biochemistry & Molecular Biology
Rosetta Ponchia, Annunziata Bruno, Asia Renzi, Claudia Landi, Enxhi Shaba, Francesca Paola Luongo, Alesandro Haxhiu, Paolo Giovanni Artini, Alice Luddi, Laura Governini, Paola Piomboni
Summary: Despite the widespread use of sperm cryopreservation, it can cause detrimental alterations in sperm function, including decreased viability, motility, and DNA fragmentation. Oxidative stress appears to play a prominent role in the mechanisms of human sperm cryodamage. Antioxidants such as myo-inositol have been shown to minimize the detrimental effects of reactive oxygen species (ROS) and improve sperm quality and motility. This study demonstrated that post-thaw addition of myo-inositol significantly improved sperm parameters, increased oxygen consumption, and decreased carbonyl groups, indicating reduced oxidative stress. These findings suggest that the developed sperm cryopreservation procedure may improve current in vitro techniques used for sperm cryostorage in assisted reproductive technology (ART) laboratories.
Article
Medicine, General & Internal
Vincenzo De Leo, Claudia Tosti, Giuseppe Morgante, Rosetta Ponchia, Alice Luddi, Laura Governini, Paola Piomboni
Summary: The study showed that a formulation containing antioxidants and energy supply substances was effective in treating sperm alterations and led to a significant recovery of fertilizing capacity.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Cell Biology
Francesca P. Luongo, Filippo Dragoni, Adele Boccuto, Eugenio Paccagnini, Mariangela Gentile, Tamara Canosi, Giuseppe Morgante, Alice Luddi, Maurizio Zazzi, Ilaria Vicenti, Paola Piomboni
Summary: This study investigates the potential of SARS-CoV-2 to infect follicular ovarian cells and finds that these cells are susceptible to the virus. This suggests that COVID-19 infection may have a detrimental effect on female fertility.
Article
Microbiology
Claudia Gandolfo, Chiara Terrosi, Shibily Prathyumnan, Gabriele Anichini, Gianni Gori Savellini, Giuseppe Morgante, Maria Grazia Cusi
Summary: This study utilized HUVECs as an in vitro model to investigate ZIKV infection, revealing that HUVECs are an optimal reservoir for viral replication and can sustain infection for up to two weeks without causing cytopathic effects. The presence of immune system components such as PMNs was found to play a crucial role in altering endothelial cell junctions, suggesting that these components may promote the spread of ZIKV in vivo in the bloodstream.
Review
Medicine, General & Internal
Giuseppe Morgante, Ilenia Darino, Amelia Spano, Stefano Luisi, Alice Luddi, Paola Piomboni, Laura Governini, Vincenzo De Leo
Summary: Recent literature highlights the importance of vitamin D (VD) in polycystic ovary syndrome (PCOS). VD deficiency is common in women with PCOS, especially those with a higher weight. Hypovitaminosis is a risk factor for glucose intolerance, insulin resistance, and increased diabetes risk. VD deficiency may also be related to hyperandrogenism in PCOS patients. VD plays a crucial role in various physiological functions and has potential effects on glucose homeostasis, hormonal status, lipid concentrations, and inflammation and oxidative stress. VD supplementation has been shown to improve insulin resistance, lipid metabolism, and response to ovulation induction in PCOS women.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Cell Biology
Alice Luddi, Francesca Paola Luongo, Filippo Dragoni, Lia Fiaschi, Ilaria Vicenti, Pietro Lupetti, Mariangela Gentile, Eugenio Paccagnini, Alesandro Haxhiu, Rosetta Ponchia, Laura Governini, Maurizio Zazzi, Paola Piomboni
Summary: Research has shown that the SARS-CoV-2 virus is highly expressed in the male reproductive system and can readily infect sperm. This suggests that the virus may have detrimental effects on sperm, highlighting the need for safety measures and guidelines in reproductive medicine.
Article
Biochemistry & Molecular Biology
Francesca Paola Luongo, Sofia Passaponti, Alesandro Haxhiu, Maryam Raeispour, Giuseppe Belmonte, Laura Governini, Livio Casarini, Paola Piomboni, Alice Luddi
Summary: This study investigates the effects of selected EDCs on hGL5, an immortalized cell line representing primary follicular granulosa cells. The results demonstrate that compounds such as BCA and caffeine significantly impact mitochondrial footprint and intracellular lipid content, suggesting a potential mechanism by which bitter taste receptors affect steroidogenesis in the female reproductive system.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)